Humic acids: The dynamics of mineralization during bioremediation by vermicomposting of soils contaminated by diesel
DOI:
https://doi.org/10.33448/rsd-v8i8.1190Keywords:
Contaminated soils; petroleum compounds; humification.Abstract
This study had as a primordium to investigate humic acids extracted from soils contaminated by compounds derived from petroleum and that have undergone bioremediation applying vermicomposting. The development of this research aims to answer the following questions: During the bioremediation was the mineralization of the substrate? The humic acids extracted from the soils showed marked differences in the chemical composition after 90 days of the bioremediation process? The justification of the research is that, to date, there have been few studies about the chemical characterization of humic acids extracted from soils that have undergone bioremediation using vermicomposting. In the dynamics of bioremediation of contaminated soils using Eisenia foetida species there is the passage of the active organic matter to its stabilized form, thus producing the humic substances. One way of monitoring the dynamics of degradation is by studying the variation of organic matter, carbon, nitrogen, and others. In order to reach the objectives, several analytical techniques were used to verify the differences in the chemical composition of the humic acids, among them to the ultraviolet/visible absorption spectroscopy and the spectroscopy in the IV. At the end, the main results show qualitative and quantitative changes in the composition of humic acids. The results show a decrease in the C / N ratio. The functional groups were the most sensitive to the changes caused by the process, evidenced by the increase in total acidity. By the infrared spectra it was possible to visualize a clear decrease in the intensity of the bands in the region 3923-2850 cm-1, corresponding to the aliphatic stretches, evidencing the dynamics of humification during bioremediation.
References
Atiyeh, R.M., Edwards, C.A., Subler, S. & Metzger, J.D. (2001). Pig manure vermicompost as a component of a horticultural bedding plant medium: effects on physicochemical proprieties and plant growth. Bioresource Technology, 78(1):11-20. https://doi.org/10.1016/S0960-8524(00)00172-3
Atiyeh, R.M., Lee, S., Edwards, C.A., Arancon, N.Q., Metzger, J.D. (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology, 84(1):7-14. https://doi.org/10.1016/S0960-8524(02)00017-2
Cotta, J. A. O., Landgraf, M.D., & Rezende, M.O.O. (2007). Validação do método para determinação de nitrogênio kjeldahl total. Revista Analytica, dezembro 2006/janeiro 2007, (26): 88-75.
Domeizel, M., Khalil, A., Prudent, P. (2004). UV spectroscopy: a tool for monitoring humification and for proposing an index of the maturity of compost. Bioresource Technology, 94(1): 177-184. https://doi.org/10.1016/j.biortech.2003.11.026
Dores-Silva, P.R., Cotta, J.A.O., Landgraf, M.D., Rezende, M.O.O. (2018). Soils impacted by PAHs: would the stabilized organic matter be a green tool for the immobilization of these noxious compounds? Journal of Environmental Science and Health, Part B. 53(5): 313-318. https://doi.org/10.1080/03601234.2018.1431461.
Dores-Silva, P.R., Cotta, J.A.O., Landgraf, M.D., Rezende, M.O.O. (2019).The application of the vermicomposting process in the bioremediation of diesel contaminated soils. Journal of Environmental Science and Health, Part B. (No prelo) Disponível em: https://doi.org/10.1080/03601234.2019.1611303
Hervas, L., Mazuelos, C., Senesi, N., Saiz-Jimenez, C. (1989). Chemical and physico-chemical characterization of vermicomposts and their humic acid fractions. Science Total Environmental, 81(1): 543-550. https://doi.org/10.1016/0048-9697(89)90162-9
Kayhanian, M. & Tchobanoglous G. (1993). Innovative two-stage process for the recovery of energy and compost from the organic fraction of municipal solid waste (MSW), Water Science and Technology, 27(1):133-143.
Kiehl, E.J. (1985). Fertilizantes orgânicos. São Paulo: Editora agronômica Ceres. 492p.
Kononova, M. M. (1966). Soil organic matter: its nature, its role in soil formation and in soil fertility. 2.ed. Oxford: Pergamon Press. 544p.
Landgraf, M.D., Silva, S.C., Rezende, M.O.O. (1998). Mechanism of metribuzin herbicide sorption by humic acid samples from peat and vermicompost. Analytica Chimica Acta, 368(1): 155-164. https://doi.org/10.1016/S0003-2670(98)00049-X
Landgraf, M.D., Alves, M.R., Silva, S.C., Rezende, M.O.O. (1999). Caracterização de ácidos húmicos de vermicomposto de esterco bovino compostado durante 3 e 6 meses. Química Nova, 22(1): 483-486.
Messias, R.A. (2004). Avaliação estrutural de ácidos húmicos de vermicomposto e turfa por diferentes técnicas de caracterização. 134f. Tese (Doutorado) - Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos/SP.
Mitchell, A. (1997). Production of Eisenia fetida and vermicompost from feed-lot cattle manure. Soil Biology Biochemistry, 29(1): 763-6. https://doi.org/10.1016/S0038-0717(96)00022-3
Pereira, A.S., Shitsuka, D.M., Parreira, F.J. & Shitsuka, R. (2018). Metodologia da pesquisa cientifica. [e-book]. Ed. UAB/NTE/UFSM. Santa Maria/RS. Disponível em:
Poppi, N.R. & Talamoni, J. (1992). Estudo dos ácidos húmico e fúlvico, extraídos de solos, por espectroscopia de infravermelho. Quimica Nova, 15(1):281-285.
Prudent, P., Domeizel, M., Massiani, C., Thomas, O. (1995). Gel chromatography separation and U.V. spectroscopic characterization of humic-like substances in urban composts. Science of The Total Environment, 172(1): 229-235. https://doi.org/10.1016/0048-9697(95)04817-0
Sanchez-Monedero, M. A., Cegarra, J., García, D., Roig, A. (2002). Chemical and structural evolution of humic acids during organic waste composting. Biodegradation, 13(1): 361–371. https://doi.org/10.1016/0048-9697(95)04817-0
Schnitzer, M. & Gupta, U.C. (1965). Determination of acidity in soil organic matter. Soil Science Society, 27(1): 274-277. doi:10.2136/sssaj1965.03615995002900030016x
Schnitzer, M. & Khan, S. U. (1972). Humic substances in the environment. New York: Marcel Dekker. p.127
Skoog, D. A., Holler, F. J., Timothy, A. N. (2002). Princípios da análise instrumental, 5. ed., Porto Alegre: Bookman. 836p.
Sposito, G. & Weber, J. H. (1986). Sorption of trace metals by humic materials in soils and natural waters. Journal Critical Reviews in Environmental Control, 16(2): 195-222. https://doi.org/10.1080/10643388609381745
Stevenson, F.J. (1994). Humus chemistry: genesis, composition, reactions. New York: John Wiley. 496p.
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