Effects of combined fungicide in stingless bees Scaptotrigona bipunctata

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.12029

Keywords:

Histology; Scanning electron microscopy; Critical electrolyte concentration; Benzimidazole; Triazole; Strobilurin.

Abstract

Locker is a fungicide used in different crops for biological control, affecting both insect pests and pollinators, such as bees. This study aimed to evaluate toxicity and establish the lethal concentration (LC50) of the fungicide Locker in stingless bees Scaptotrigona bipunctata, investigating changes in brain cells chromatin structure and in midgut morphology. This agrochemical is a combined fungicide that contains three active ingredients: benzimidazole, triazole and strobilurin. Adult forage bees were orally exposed to the following concentrations: 1.7 mg a.i./L; 2.55 mg a.i./L; 3.4 mg a.i./L and 4.25 mg a.i./L of fungicide, and submitted to histochemical and morphological analysis 24, 48 and 72 hours after ingestion. The analysis of S. bipunctata brain cells showed changes in chromatin condensation in all treatments. There were also several morphological changes in the midgut at all concentrations and exposure times. Thus, the fungicide Locker significantly affected bee survival, inducing changes in their internal morphology, which can lead to alterations in their activities, directly interfering in their foraging behavior and survival.

Author Biographies

Tamiris de Oliveira Diniz, Universidade Estadual de Maringá

Biology degree at Universidade Paranaense (Unipar). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). Specialist in Biotechnology at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Naiara Climas Pereira, Universidade Estadual de Maringá

Biology degree at the Universidade Estadual de Maringá (UEM). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Breno Gabriel da Silva, Universidade de São Paulo

Mathematics degree at Universidade Estadual de Maringá (UEM). Master in Biostatistics at Universidade Estadual de Maringá (UEM). PhD student in Statistics and Agricultural Experimentation at Escola Superior de Agricultura "Luiz de Queiroz" - Universidade de São Paulo (ESALQ / USP).

William Cristian da Silva Pizzaia, Universidade Estadual de Maringá

Biology degree at Faculdade Global de Umuarama (FGU). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Fernanda Giovana Martins de Oliveira, Universidade Estadual de Maringá

Biology degree at Faculdade de Jandaia do Sul (FAFIJAN). Master's student in Genetics and Breeding at at Universidade Estadual de Maringá (UEM).

Adriana Aparecida Sinópolis-Gigliolli, Universidade Estadual de Maringá

PhD in Biological Sciences at Universidade Estadual de Maringá (UEM). Adjunct professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

Vagner de Alencar Arnaut de Toledo, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP). Full professor at the Zootechnics Department at Universidade Estadual de Maringá (UEM).

Maria Claudia Colla Ruvolo-Takasusuki, Universidade Estadual de Maringá

PhD in Genetics and Evolution at Universidade Federal de São Carlos (UFSCAR). Associate professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

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Published

30/01/2021

How to Cite

DINIZ, T. de O.; PEREIRA, N. C.; SILVA, B. G. da; PIZZAIA, W. C. da S.; OLIVEIRA, F. G. M. de .; SINÓPOLIS-GIGLIOLLI, A. A. .; TOLEDO, V. de A. A. de .; RUVOLO-TAKASUSUKI, M. C. C. . Effects of combined fungicide in stingless bees Scaptotrigona bipunctata . Research, Society and Development, [S. l.], v. 10, n. 1, p. e53710112029, 2021. DOI: 10.33448/rsd-v10i1.12029. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12029. Acesso em: 28 feb. 2021.

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Section

Agrarian and Biological Sciences