Milhocin as protein source for bacteriocin production by Enterococcus faecium

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12206

Keywords:

Enterocin; Maceration of corn; Food preservation; Enterococcus sp.

Abstract

The production of bacteriocin by Lactic Acid Bacteria has attracted great attention because of its GRAS (Generally Recognized as Safe) status, and its potential use as an additive for food preservation. The use of substrate with characteristics that provide necessary nutrients, but at a low cost, is among the essential items for the production and commercial use of bacteriocins. Therefore, the objective of this work was to evaluate the viability of the production of enterokines, using whey and corn maceration water (millhocin) as substrate in 18 and 24 hours of cultivation. Enterococcus faecium (Efm20, Efm22, Efm24, Efm25) and Enterococcus faecalis (Efs27) were characterized for the antimicrobial activity of enterocins present in the cell-free supernatant (CFS) against Listeria innocua CLIP 12612. The protein character of the antimicrobial substance was confirmed , its inhibitory activity being lost after the treatment of CFS with proteases. In contrast, the CFS antimicrobial activity remained thermostable after heat treatment (80 ° C and 100 ° C) and also when treated with catalase, which confirms that this CFS activity is not related to the action of H2O2 and lactic acid (CFS neutralized with NaOH). Counting bacterial cells (CFU / mL) of isolates grown in different media, we  observed increase of CFU in MRS with substrates. The antimicrobial activity of CFS obtained from MRS media with substrate compared to MRS was similar when diffusing in agar, however, when quantifying this activity, it observed higher values of arbitrary unit for CFS obtained in MRS medium with millhocin at both times evaluated 18 and 24 hours. In conclusion, it was found that the tested substrates have great potential to be applied in the manufacture of bioconservation of food products.

References

Amartey, A., & Leung, J. (2000). Corn steep liquor as a source of nutrients for ethanologic fermentation by Bacillus stearothermophilus t-13. Bulletin of the Chemists and Technologists, 19.

Ammor, S., Tauveron, G., Dufour, E. & Chevallier, I. (2006). Antibacterial Activity of Lactic Acid Bacteria against Spoilage and Pathogenic Bacteria Isolated from the Same Meat Small-Scale Facility. 1 - Screening and Characterization of the Antibacterial Compounds. Food Control, 17(6), 454–461.

Ananou, S., Muñoz, A., Gálvez, A., Martínez-Bueno, M. & Maqueda, M. (2008). Optimization of Enterocin AS-48 Production on a Whey-Based Substrate. International Dairy Journal, 18(9), 923–927.

Bromberg, R., Moreno, I., Delboni, R. R & Cintra, H. C. (2006). Características da bacteriocina produzida por Lactococcus lactis ssp. hordniae CTC 484 e seu feito sobre Listeria monocytogenes em carne bovina. Ciência Tecnologia Alimentos, 26(1), 135-144.

Cleveland, J., Montville, T. J.., Nes, I. F. & Chikindas, M. L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. Int J Food Microbiol, 71(1):1–20.

Dalié, D. K. D., Deschamps, A. M. & Richard-Forget, F. (2010). Bactérias de ácido láctico - Potencial para controle do crescimento de fungos e micotoxinas: Uma revisão. Food Control, 21, 370-380.

Du, L., Liu, F., Zhao, P., Zhao, T. & Doyle, M. P. (2017). Characterization of Enterococcus durans 152 bacteriocins and their inhibition of Listeria monocytogenes in ham. Food Microbiology, 68, 97–103.

Ferreira, A., Canal, N., Morales, D., Bopp, D. & Corção, G. (2007). Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces. Braz Arch Biol Technol, 2(50), 249–258.

Fouquiér-Moreno, M. R., Callewaert, R., Devreese, B., Van Beeumen, J. & De Vuyst, L. (2003). Isolation and biochemical characterisation of enterocins produced by enterococci from different sources. Journal of Applied Microbiology, 94, 214–229.

Furlaneto-Maia, L., Ramalho, R., Rocha, K. R. & Furlaneto, M. C. (2020). Antimicrobial activity of enterocins against Listeria sp. and other food spoilage bacteria. Biotechnol Lett. doi.org/10.1007/s10529-020-02810-7.

Garriga, M., Hugas, M., Aymerich, T. & Monfort, J. M. (1993). Bacteriocinogenic activity of lactobacilli from fermented sausages. Journal of Applied Microbiology, 75, 142-148.

Ghrairi, T., Frere, J. J., Berjeaud, M. & Manai, M. (2008). Purification and Characterisation of Bacteriocins Produced by Enterococcus Faecium from Tunisian Rigouta Cheese. Food Control, 19(2), p. 162–169.

Hu, Y., Liu, X., Shan, C., Xia, X., Wang, Y., Dong, M. & Zhou, J. (2017). Novel bacteriocin produced by Lactobacillus alimentarius FM -MM 4 from a traditional Chinese fermented meat Nanx Wudl: Purification, identification and antimicrobial characteristics, Food Control, 77, 290 -297.

Khan, H., Flint, S. & Yu, P.L. (2010). Enterocins in food preservation. International Journal of Food Microbiology, 141, 1-10.

López, R. S., García, M. T., Abriouel H., Omar, N. B., Martínez-Cañamero, M. & Gálvez, A. (2007). Semi-Preparative Scale Purification of Enterococcal Bacteriocin Enterocin EJ97, and Evaluation of Substrates for Its Production. Journal of Industrial Microbiology and Biotechnology, 34(12),779–785.

Lü, X., Yi, L., Dang, J., Dang, Y e Liu, B. (2014). Purification of novel bacteriocin produced by Lactobacillus coryniformis MXJ 32 for inhibiting bacterial foodborne pathogens including antibiotic -resistant microorganisms, Food Control, 46, 264 -271.

Luna, J. M., Rufino, R. D., Sarubbo, L. A. & Campos, G. M. (2008). Produção de Biossurfactante em meio de baixo custo formulado com água do mar. Exacta, 6(2), 209 – 215.

Mayr-Harting, A., Hedges, A. J. & Berkeley, R. C. W. (1972). Methods for studying bacteriocins. Methods in Microbiology, 7A(4), 315-422.

Motta, A. S. & Brandelli, A. (2008). Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34. World Journal of Microbiology and Biotechnology, 24(5), 641–646.

Oliveira, C. P. J., Siqueira-Júnior, P. & Silva, J. A. (2012). Bacteriocinas Como Alternativa Na Conservação De Alimentos. Revista Verde de Agroecologia e Desenvolvimento Sustentável, 7(1), 09–15.

Ogaki, M. B., Rocha, K. R., Terra, M. R., Furlaneto, M. C. & Furlaneto- Maia, L. (2016). Screening of the enterocin-encoding genes and antimicrobial activity in Enterococcus species. J Microbiol Biotechnol, 26:1026–1034.

Ozdemir, G. B., Oryasin, E., Biyik, H. H., Ozteber, M. & Bozdogan, B. (2011). Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian J Microbiol, 51(2):182–7.

Rivas, B., Moldes, A. B.., Domínguez, J. M. & Parajó, J. C. (2004). Development of Culture Media Containing Spent Yeast Cells of Debaryomyces Hansenii and Corn Steep Liquor for Lactic Acid Production with Lactobacillus Rhamnosus. International Journal of Food Microbiology, 97(1), 93–98.

Rocha, K. R., Perini, H. F., Souza, C. M., Schueler, J., Tosoni, N. F., Furlaneto, M. C. & Furlaneto‑Maia, L. (2019). Inhibitory effect of bacteriocins from enterococci on developing and preformed biofilms of Listeria monocytogenes, Listeria ivanovii and Listeria innocua. World Journal of Microbiology and Biotechnology 35:96.

Salvucci, E., Rossiet, M., Colombo, A., Pérez, G., Borneo, R. & Aguirre, A. (2019). Triticale flour films added with bacteriocin-like substance (BLIS) for active food packaging applications. Food Packaging and Shelf Life, 19, 193–199.

Tosoni, N. F., Perini, H. F., Terra, M. R., Katsuda, M. S., Furlaneto, M. C. & Furlaneto-Maia, L. (2019) Antimicrobial activity of enterocin obtained from Enterococcus durans on Shiga-like toxin-producing Escherichia coli. Ciência Rural, Santa Maria, 49(9), e20190297.

Vázquez, J. A., González, M. P. & Murado, M. A. (2006). Preliminary Tests on Nisin and Pediocin Production Using Waste Protein Sources: Factorial and Kinetic Studies. Bioresource Technology , 97(4), 605–613.

Yang, E., Lihua, F., Jiang, Y., Doucette, C. & Fillmore, , S. (2012). Atividade antimicrobiana de bactérias lácticas produtoras de bacteriocinas isoladas de queijos e iogurtes. AMB Express, v.2,

Zendo, T., Koga, S., Shigeri, Y., Nakayama, J. & Sonomoto, K. (2006). Lactococcin Q, a novel two-peptide bacteriocin produced by Lactococcus lactis QU 4. Appl Environ Microbiol, 72(5):3383–3389.

Published

02/02/2021

How to Cite

SCHUELER, J. .; SOUZA, C. M. de .; KATSUDA, M. S. .; FURLANETO, M. C. .; FURLANETO-MAIA, L. Milhocin as protein source for bacteriocin production by Enterococcus faecium. Research, Society and Development, [S. l.], v. 10, n. 2, p. e1110212206, 2021. DOI: 10.33448/rsd-v10i2.12206. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12206. Acesso em: 26 nov. 2024.

Issue

Section

Agrarian and Biological Sciences