Effect of hydroalcoholic neem (Azadirachta indica) extract and oil on the fungus Fusarium oxysporum f. sp. vasinfectum and in the induced resistance of okra to fusariosis

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12357

Keywords:

Abelmoschus esculentus; ROS; Wilt; SAR.

Abstract

The objective of this work was to verify the effect of hydroalcoholic extract and neem oil on mycelial growth, sporulation, germination, and conidia adhesion of the fungus F. oxysporum f. sp. vasinfectum. Also, to evaluate the effect of the extract and oil mentioned in the induction of resistance in treated okra trees and, later, inoculated with the fungus, through the activity of antioxidant enzymes related to plant defense processes. The results showed that both the extract and the neem oil were efficient in inhibiting mycelial growth and sporulation of the fungus. In addition, there was inhibition of germination and fixation of conidia produced in the presence of the products. Neem extract and oil reduced the severity of the disease in okra, as measured by the disease’s infection rate. In addition, significant increases in the enzymes guaiacol-peroxidase, polyphenoloxidase, superoxide dismutase and catalase were observed in the okra treated with the products, these increases being directly proportional to the increases in the concentrations used. Thus, both the extract and the neem oil have direct effects on the development of the fungus F. oxysporum f. sp. vasinfectum and induce resistance in okra from the formation of reactive oxygen species (ROS).

Author Biography

Thiago Anchieta de Melo, Universidade Estadual do Maranhão

Agricultural Engineer, Degree in Biology, Doctor of Science (Phytopathology), from the University of São Paulo (ESALQ / USP); Professor of Microbiology in the courses of Biological Sciences, Agronomic Engineering and Zootechnics and of the disciplines Biology of Fungi, Diversity of Microorganisms, Environmental Microbiology and Fundamentals in Biotechnology offered to the Biological Sciences course at the State University of Maranhão (UEMA), Campus São Luís. Has experience in the field of Agronomy / Biology / Microbiology / Phytopathology with emphasis on Physiology of Parasitism of the pathogen-host interaction and Induction of Resistance of Vegetables, especially fruits and vegetables in the post-harvest phase.

References

Akhila, A., & Rani, K. (1999). Chemistry of the neem tree (Azadirachta indica A. Juss.). Fortschritte der Chemie organischer Naturstoffe/Progress in the Chemistry of Organic Natural Products, 47-149.

Asada, K., Takahashi, M. A., & Nagate, M. (1974). Assay and inhibitors of spinach superoxide dismutase. Agricultural and biological chemistry, 38(2), 471-473.

Baker, C. J., & Orlandi, E. W. (1995). Active oxygen in plant pathogenesis. Annual review of phytopathology, 33(1), 299-321.

Bedendo, I. P. (2011). Murchas vasculares. In: Amorim L., Rezende J. A. M., Bergamin Filho A. (Eds.) Manual de Fitopatologia: princípios e conceitos. São Paulo: Ceres.

Beers, R. F., & Sizer, I. W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol chem, 195(1), 133-140.

Beltrame, A. B. (2010). Interação Phytophthora nicotianae-porta-enxerto de citros (tangerina Sunki e citrumelo Swingle): efeito no sistema radicular, aspectos fisiológicos e bioquímicos (Doctoral dissertation, Universidade de São Paulo).

Bhuvaneswari, V., Srivastava, A. K., & Paul, P. K. (2012). Aqueous fruit extracts of Azadirachta indica induce systemic acquired resistance in barley against Drechslera graminea. Archives of Phytopathology and Plant Protection, 45(8), 898-908.

Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U (2002) Biological activities and medicinal properties of neem (Azadirachta indica). Current Science 82(11):1336-1345.

Brahmachari G (2004) Neem – an omnipotent plant: a retrospection. Chembiochem 5(4):408-421.

Carvalho, J. R., Setubal, J. W., de Sousa, R. L., Mota, P. R. D. A., Alves, T. N., da Silva, V. B., ... & de Assis, H. J. C. (2020). Cultivares de quiabeiro sob diferentes polinizações e seus efeitos na qualidade física e fisiológica das sementes. Brazilian Journal of Development, 6(12), 94728-94738.

Cia E., Grid-Papp, L. L., Soave, J., Ferraz, C. A. M. (1977). Resistência de Novos Cultivares de Algodoeiro a Fusarium oxysporum f. sp. vasinfectum e a Xanthomonas malvaceaum. Summa Phytopathologica, 3, 260-270.

Cilo, B. D., Al-Hatmi, A. M., Seyedmousavi, S., Rijs, A. J., Verweij, P. E., Ener, B., ... & van Diepeningen, A. D. (2015). Emergence of fusarioses in a university hospital in Turkey during a 20-year period. European Journal of Clinical Microbiology & Infectious Diseases, 34(8), 1683-1691.

Dean, R., Van Kan, J. A., Pretorius, Z. A., Hammond‐Kosack, K. E., Di Pietro, A., Spanu, P. D., ... & Foster, G. D. (2012). The Top 10 fungal pathogens in molecular plant pathology. Molecular plant pathology, 13(4), 414-430.

Dheeba, B., Niranjana, R., Sampathkumar, P., Kannan, K., & Kannan, M. (2015). Efficacy of neem (Azadirachta indica) and tulsi (Ocimum sanctum) leaf extracts against early blight of tomato. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 85(1), 327-336.

Doshi, P., Nisha, N., Yousif, A. I. A., Körösi, K., Bán, R., & Turóczi, G. (2020). Preliminary Investigation of Effect of Neem-Derived Pesticides on Plasmopara halstedii Pathotype 704 in Sunflower under In Vitro and In Vivo Conditions. Plants, 9(4), 535.

Duangmal, K., & Apenten, R. K. O. (1999). A comparative study of polyphenoloxidases from taro (Colocasia esculenta) and potato (Solanum tuberosum var. Romano). Food Chemistry, 64(3), 351-359.

Duong, D. H., Ngo, X. Q., Do, D. G., Le, T. A. H., Nguyen, V. T., & Nic, S. (2015). Effective control of neem (Azadirachta indica A. Juss) cake to plant parasitic nematodes and fungi in black pepper diseases in vitro. Journal of Vietnamese Environment, 6(3), 233-238.

El-Ghany, A., Roushdy, M. M., & Mohamed, A. A. (2015). Efficacy of certain plant extracts as safe fungicides against phytopathogenic and mycotoxigenic fungi. Agric. Biol. Sci. J, 1(3), 71-75.

FILGUEIRA, F. (2008). Novo Manual de Olericultura: agrotecnologia moderna na produção e comercialização de hortaliças, 3ª edição. Viçosa: UFV.

Goel, N., Anukrati, K., & Paul, P. K. (2016). Anti-phytopathogenic and SAR inducing properties of neem: A review. Journal of Chemical and Pharmaceutical Sciences, 9(4), 2547-2555.

Guleria, S., & Kumar, A. (2006). Azadirachta indica leaf extract induces resistance in sesame against Alternaria leaf spot disease. Journal of Cell and Molecular Biology, 5(2), 81-86.

Gupta, A. S., Webb, R. P., Holaday, A. S., & Allen, R. D. (1993). Overexpression of superoxide dismutase protects plants from oxidative stress (induction of ascorbate peroxidase in superoxide dismutase-overexpressing plants). Plant Physiology, 103(4), 1067-1073.

Gupta, S. C., Prasad, S., Tyagi, A. K., Kunnumakkara, A. B., & Aggarwal, B. B. (2017). Neem (Azadirachta indica): An indian traditional panacea with modern molecular basis. Phytomedicine, 34, 14-20.

Hanaa, R. F., Abdou, Z. A., Salama, D. A., Ibrahim, M. A., & Sror, H. A. M. (2011). Effect of neem and willow aqueous extracts on Fusarium wilt disease in tomato seedlings: Induction of antioxidant defensive enzymes. Annals of Agricultural Sciences, 56(1), 1-7.

Hao, F., Kumar, S., Yadav, N., & Chandra, D. (2014). Neem components as potential agents for cancer prevention and treatment. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1846(1), 247-257.

Hof, H. (2020). The Medical Relevance of Fusarium spp. Journal of Fungi, 6(3), 117.

Imtiaj, A., Rahman, S. A., Alam, S., Parvin, R., Farhana, K. M., Kim, S. B., & Lee, T. S. (2005). Effect of fungicides and plant extracts on the conidial germination of Colletotrichum gloeosporioides causing mango anthracnose. Mycobiology, 33(4), 200-205.

Itako, A. T., Schwan-Estrada, K. R. F., Stangarlin, J. R., Tolentino Júnior, J. B., & Cruz, M. E. S. (2009). Controle de Cladosporium fulvum em tomateiro por extratos de plantas medicinais. Arquivos do Instituto Biológico, 76(1), 75-83.

James, S. P., Lond, M. B. (1903) The basil and the neem. British Medical Journal, 667.

Kagale, S., Marimuthu, T., Thayumanavan, B., Nandakumar, R., & Samiyappan, R. (2004). Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiological and Molecular Plant Pathology, 65(2), 91-100.

Kamali, N., Pourjam, E., & Sahebani, N. (2015). Elicitation of defense responses in tomato against Meloidogyne javanica and Fusarium oxysporum f. sp. Lycopersici wilt complex. Journal of Crop Protection, 4(1), 29-38.

Lamb, C. J., Lawton, M. A., Dron, M., & Dixon, R. A. (1989). Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell, 56(2), 215-224.

Locke, J. E. (1995). Fungi. In: Schmutterer, H. (Ed.) The Neem Tree, source of Unique National Products for Integrated pest Management, Medicine, Industry and Other proposes. Germany: VCH Weinheim.

Lorencetti, G. A. T., Mazaro, S. M., Potrich, M., Lozano, E. R., Barbosa, L. R., Luckmann, D., & Dallacort, S. (2015). Alternative Products for Thaumastocoris peregrinus Control and Resistance Induction in Plants. Floresta e Ambiente, 22(4), 541-548.

Matiello, J., Raasch-Fernandes, L. D., Berber, G. D. C. M., Trento, R. A., & Bonaldo, S. M. (2016). Síntese de Fitoalexinas em Soja e Sorgo por Extratos e Tinturas Pertencentes a Três Espécies Florestais. Revista em Agronegócio e Meio Ambiente, 9(3), 617-633.

McKinney, H. H., & Davis, R. J. (1925). Influence of soil temperature and moisture on infection of young wheat plants by Ophiobolus graminis. Journal of Agricultural Research, 31(9), 827-840.

Melo, T. A. D. (2017). Efeito do extrato da alga marinha Ascophyllum nodosum e do fosfito de potássio na morfofisiologia do fungo Colletotrichum gloeosporioides, na indução de resistência em mangas “Tommy Atkins” contra a antracnose e em características físicas e químicas desses frutos (Doctoral dissertation, Universidade de São Paulo).

Melo, T. A., Araújo, M. U. P., Serra, I. M. R. D. S., & Pascholati, S. F. (2017). Commercially available natural products induce phytoalexin accumulation in soybean cotyledons and sorghum mesocotyls. Summa Phytopathologica, 43(3), 205-211.

Menezes, M., & Assis, S. M. P. (2004). Guia prático para fungos fitopatogênicos. 2ª edição, Recife: UFRPE Imprensa Universitária.

Mielniczuk, E., & Skwaryło-Bednarz, B. (2020). Fusarium Head Blight, mycotoxins and strategies for their reduction. Agronomy, 10(4), 509.

Miya, M. D., & Shamsi, S. (2017). In Vitro Evaluation of Selected Plant extracts and Chemicals against Pathogenic Fungi isolated from Momordica Charantia L. Journal of Bangladesh Academy of Sciences, 41(1), 11-16.

Mota, W. F., Oliveira, G. B., Nobre, S. A., & Silva, H. R. (2017). Agronomic performance of okra under the effect of seed microbiolization with rhizobacteria. Horticultura Brasileira, 35(3), 410-414.

Moura, A. P., & Guimaraes, J. (2014). Manejo de pragas na cultura do quiabeiro. Embrapa Hortaliças-Circular Técnica (INFOTECA-E).

Nisha, S., Revathi, K., Chandrasekaran, R., Kirubakaran, S. A., Sathish-Narayanan, S., Stout, M. J., & Senthil-Nathan, S. (2012). Effect of plant compounds on induced activities of defense-related enzymes and pathogenesis related protein in bacterial blight disease susceptible rice plant. Physiological and molecular plant pathology, 80, 1-9.

Patel, S. M., Venkata, K. C. N., Bhattacharyya, P., Sethi, G., & Bishayee, A. (2016, October). Potential of neem (Azadirachta indica L.) for prevention and treatment of oncologic diseases. In Seminars in cancer biology (Vol. 40, pp. 100-115). Academic Press.

Paul, P. K., & Sharma, P. D. (2002). Azadirachta indica leaf extract induces resistance in barley against leaf stripe disease. Physiological and molecular plant pathology, 61(1), 3-13.

Santos, R., Pires, T. P., Mesquita, M. L. R., Correa, M. J. P., & Silva, M. R. M. (2020). Weed interference in okra crop in the organic system during the dry season. Planta Daninha, 38.

Roncatto, M. C., & Pascholati, S. F. (1998). Changes in activity and electrophoretic pattern of peroxidase in maize (Zea mays) and sorghum (Sorghum bicolor) leaves treated with yeast (Saccharomyces cerevisae). Scientia Agricola, 55, 395-402.

Sampaio, A. M., Araújo, S. D. S., Rubiales, D., & Vaz Patto, M. C. (2020). Fusarium wilt management in legume crops. Agronomy, 10(8), 1073.

Schiwek, S., Beule, L., Vinas, M., Pfordt, A., von Tiedemann, A., & Karlovsky, P. (2020). High-Resolution Melting (HRM) Curve Assay for the Identification of Eight Fusarium Species Causing Ear Rot in Maize. Pathogens, 9(4), 270.

Sengupta, P., Raman, S., Chowdhury, R., Lohitesh, K., Saini, H., Mukherjee, S., & Paul, A. (2017). Evaluation of apoptosis and autophagy inducing potential of Berberis aristata, Azadirachta indica, and their synergistic combinations in parental and resistant human osteosarcoma cells. Frontiers in oncology, 7, 296.

Siah, A., Magnin-Robert, M., Randoux, B., Choma, C., Rivière, C., Halama, P., & Reignault, P. (2018). Natural agents inducing plant resistance against pests and diseases. In Natural antimicrobial agents (pp. 121-159). Springer, Cham.

Siddiqui, S., Mahmood, T., Siddiqui, B. S., & Faizi, S. (1986). Two new tetranortriterpenoids from Azadirachta indica. Journal of Natural Products, 49(6), 1068-1073.

Subapriya, R., & Nagini, S. (2005). Medicinal properties of neem leaves: a review. Current Medicinal Chemistry-Anti-Cancer Agents, 5(2), 149-156.

Tahir, H. A., Gu, Q., Wu, H., Raza, W., Hanif, A., Wu, L., ... & Gao, X. (2017). Plant growth promotion by volatile organic compounds produced by Bacillus subtilis SYST2. Frontiers in Microbiology, 8, 171.

Terra, W. C., Campos, V. P., Pedroso, M. P., da Costa, A. L., Freire, E. S., de Pinto, I. P., ... & Santos, T. C. N. (2017). Volatile molecules of Fusarium oxysporum strain 21 are retained in water and control Meloidogyne incognita. Biological Control, 112, 34-40.

Toledo, G. L., & Ovalle, I. I. (2008). Estatística Básica. São Paulo. Atlas.

Tumen, I., Eller, F. J., Clausen, C. A., & Teel, J. A. (2013). Antifungal activity of heartwood extracts from three Juniperus species. BioResources, 8(1), 12-20.

van Diepeningen, A. D., & de Hoog, G. S. (2016). Challenges in Fusarium, a trans-kingdom pathogen. Mycopathologia, 181(3-4), 161-163.

Wang, J., Li, J., Cao, J., & Jiang, W. (2010). Antifungal activities of neem (Azadirachta indica) seed kernel extracts on postharvest diseases in fruits. African Journal of Microbiology Research, 4(11), 1100-1104.

Published

04/02/2021

How to Cite

MELO, T. A. de; SERRA, I. M. R. de S.; NASCIMENTO, I. T. V. da S. do. Effect of hydroalcoholic neem (Azadirachta indica) extract and oil on the fungus Fusarium oxysporum f. sp. vasinfectum and in the induced resistance of okra to fusariosis. Research, Society and Development, [S. l.], v. 10, n. 2, p. e7110212357, 2021. DOI: 10.33448/rsd-v10i2.12357. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12357. Acesso em: 17 apr. 2024.

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Section

Agrarian and Biological Sciences