Biophototic in azuki bean seeds treated with ultrahigh dilutions

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12462

Keywords:

Homeopathy; Gas discharge visualization; Biospeckle laser; Vigna angularis.

Abstract

Seed analysis is usually performed by destructive samples with time-consuming methods. Treatments that improve seed vigor are suggested based on bioassays and protocols for most plants. This includes the use of images to assess quality and pro- vide information that supports decisions. However, instrumental cost has not made these technologies widely used. The objective of this research was to study biophotonic images generated by low cost technologies - cold plasma scanner or gas discharge visualization (GDV) and speckle laser (BSL) - to evaluate the germination of adzuki beans seeds treated with ultrahigh dilutions (UHD). The research was carried out at the EPAGRI Homeopathy and Plant Health Laboratory, with a post-graduate support at the State University of Santa Catarina (UDESC). The images of the seeds were diagnosed mathematically, before and after germination, using “Local Connected Fractal Dimension Analysis” (LCFD) and “Time History Speckle Pattern” (THSP). The results show that the images identify the effects of high dilution - Bryonia alba and Silicea terra, at 6, 12 and 30 CH (centesimal hahnemannian dilution order) - on the seeds of azuki beans. There were statistically differences (p < 1% and p < 5%) of the treatments in relation to the control: water. Only Silicea terra 30CH inhibited seed germination. The signals found in the BSL images of the seeds were superior to the GDV signals, showing an average of 89% correct responses (BSL) against 87% (GDV).

References

Arizaga, R., Trivi, M. & Rabal. (1999). Speckle time evolution characterization by the co-occurrence matrix analysis. Optics & Laser Technology 31, 163–9. https://doi.org/10.1016/S0030-3992(99)00033-X

Bajpai, R., Bajpai, P. & Roy, D. (1991). Ultraweak photon emission in germinating seeds: a signal of biological order. Journal of bioluminescence and chemiluminescence 6, 227–30. https://doi.org/10.1002/bio.1170060403

Betti, L., Trebbi, G., Kokornaczyk, M.O., Nani, D., Peruzzi, M., Dinelli, G., Bellavite, P. & Brizzi, M. (2017). Number of succussion strokes affects effectiveness of ultra-high- diluted arsenic on in vitro wheat germination and polycrystalline structures obtained by droplet evaporation method. Homeopathy 26, 47–54. https://doi.org/10.1016/j.homp.2016.12.001

Bonfim, F., Casali, V., Mendonca, E. & Martins, E. (2012). Estresse hı́drico em feijoeiro (Phaseolus vulgares L.) tratado com os preparados homeopáticos de Arnica montana. Enciclopédia biosfera 8, 530–8.

Braga, R. A., Enes, A. M., Miron, D., Rabelo, G. F., J. Barreto Filho, B. & Horgan, G. W. (2007). Biological feature isolation by wavelets in biospeckle laser images. Computers and electronics in agriculture 58, 123–32. https://doi.org/10.1016/j.compag.2007.03.009

Braga, R. A., Rivera, F.P. & Moreira, J. (2016). A practical guide to Biospecle Laser analysis: theory and Software. Ed. UFLA.

Deekshitulu, B. P. (2019). Impact of Homeopathy in Agriculture. Global Journal of Energy and Environment 1, 2. Review Article.

Elias, S. G., Copeland, L. O., McDonald, M. B. & Baalbaki, R. Z. (2012). Seed testing: principles and practices. Michigan State University Press.

Fujii, H., Nohira, K., Yamamoto, Y., Ikawa, H. & Ohura, T. (1987). Evaluation of blood flow by laser speckle image sensing. Part 1. Applied Optics 26, 5321–5. https://doi.org/10.1364/AO.26.005321

Hammer, Ø., Harper, D. & Ryan, P. (2019). PAST: paleontological statistics. Version 3.25. National History Museum, University of Oslo.

Karperien, A., Jelinek, H., Milosevic, N. & Cracow, P. (2011, June). Reviewing lacunarity analysis and classification of microglia in neuroscience. Proceedings of the 8th European Conference on Mathematical and Theoretical Biology European Society for Mathematical and Theoretical Biology (ESMTB) 2011 MS#88. DOI: 10.13140/2.1.3576.6082.

Kokornaczyk, M. O., Trebbi, G., Dinelli, G., Marotti, I., Bregola, V., Nani, D., Borghini, F. & Betti, L. (2014). Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultrahigh dilutions. Complementary therapies in medicine 22, 333–40. https://doi.org/10.1016/j.ctim.2014.02.005

Kononenko, I., Bevk, M., Sadikov, S. & Sajn, L. (2004). Classification of different types of coronas using parametrization of images and machine learning. In: Konstantin G. Korotkov (Ed.). Measuring Energy Fields: Current Research. – Backbone Publishing Co. Fair Lawn, USA, pp. 193-208.

Korotkov, K. Measuring Energy Fields: State of the Science, Backbone Publishing, Fair Lawn, USA. pp. 278, 2004.

Korotkov, K. G., Matravers, P., Orlov, D. V. & Williams, B. O. (2010). Application of electrophoton capture (EPC) analysis based on gas discharge visualization (GDV) technique in medicine: a systematic review. The Journal of Alternative and Complementary Medicine 16, 13–25.

Li, H., Li, Z. & Qiu, Y. (2006). Fractal analysis of laser speckle for measuring roughness. ICO20: Optical Information Processing, edited by Yunlong Sheng, Songlin Zhuang, Yimo Zhang, Proc. of SPIE Vol. 6027, 60271S, 0277-786X/06/$15. https://doi.org/10.1117/12.668182

Marques, R. M., Bonato, C. M. & Mourão, K. S. M. (2013). Effect of homeopathic drug Bryonia alba on oxygen uptake by alternative oxidase pathway in soybean. in II International Conference on Homeopathy in Agriculture. pp 4.

Matzrafi, M., Herrmann, I., Nansen, C., Kliper1, T., Zait, Y., Ignat, T., Siso, D., Rubin, B., Karnieli, A. & Eizenberg, H. (2017). Hyperspectral technologies for assessing seed germination and trifloxysulfuron-methyl response in Amaranthus palmeri (Palmer Amaranth). Frontiers in plant science 8, 474. https://doi.org/10.3389/fpls.2017.00474

Ministério da Agricultura, Pecuária e Abastecimento. (2009). Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Brasília. Mapa/ACS, 2009. 399 p.

Ministério da Agricultura, Pecuária e Abastecimento. (2014). Instrução Normativa Nº 17, de 18 de junho de 2014. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Http://www.agricultura.gov.br/assuntos/sustentabilidade/organicos/legislacao/portugues/instrucao- normativa-no-17-de-18-de-junho-de-2014.pdf. [Online].

Minkin, V. A. & Nikolaenko, N. N. (2008). Application of Vibraimage technology and system for analysis of motor activity and study of functional state of the human body. Biomedical Engineering. St Petersburg, 42(4), 1296-200. https://doi.org/10.1007/s10527-008-9045-9

Orjales, I., López-Alonso, M., Rodríguez-Bermúdez, R., Rey-Crespo, F., Villar, A. & Miranda, M. (2016). Use of homeopathy in organic dairy farming in Spain. Homeopathy 105, 102–8. https://doi.org/10.1016/j.homp.2015.08.005

Panda, S. S., Mohanty, S. S. & Dhal, N. K. (2013). Effects of potentized homeopathic medicines on the germination, growth and photosynthetic activity of Pisum sativum L. Recent research in Science and Technology 5, 11-14.

Pieczywek, P., Cybulska, J., Szymańska-Chargot, M., Siedliska, A., Zdunek. A., Nosalewicz, A., Baranowski, P. & Kurenda, A. (2018). Early detection of fungal infection of stored apple fruit with optical sensors–Comparison of biospeckle, hyperspectral imaging and chlorophyll fluorescence. Food control 85, 327–38. https://doi.org/10.1016/j.foodcont.2017.10.013

Rabal, H., Grumel, E., Cap, N., Buffarini, L. & Trivi, M. (2018). A descriptor of speckle textures using box fractal dimension curve. Optics and Lasers in Engineering 106, 47–55. https://doi.org/10.1016/j.optlaseng.2018.02.006

Rabelo, G. F., Enes, A. M., Junior, R. A. B. & Fabbro, I. M. Dall. (2011). Frequency response of biospeckle laser images of bean seeds contaminated by fungi. Biosystems engineering 110, 297–301. https://doi.org/10.1016/j.biosystemseng.2011.09.002

Riva, N. da, Donato, G., Kagami, F. L., Marques, R. M., Bonato, C. M. & Mourão, K. S. M. (2013, September). Effect of homeopathic drug Bryonia alba on respiration of embryo and root apices in Glycine max L. Merrill. cv. BRS 232. Annals of International II Conference on Homeopathy in Agriculture, Maringá, PR, Brazil.

Sahoo, P. K. & Arora, G. (2004). A thresholding method based on two-dimensional Renyi’s entropy. Pattern Recognition 37, 1149–61. https://doi.org/10.1016/j.patcog.2003.10.008

Schulte, J. & Endler, P. C. (2015). Update on preliminary elements of a theory of ultra high dilutions. Homeopathy 104, 337–42. https://doi.org/10.1016/j.homp.2015.09.010

Shtam, A. I., Minkin, V. A. & Korotkov, K. G. (1997). Dispositivo para visualização de imagem por descarga de gás. Patente BR9714982A.1997.

Silva, H. A da, Parizotto, A. V., Moreira, F. C., Marques, R. A., Reis, B., & Bonato, C. M. (2012). The effect of high dilutions of Pulsatilla nigricans on the vigour of soybean seeds subjected to accelerated aging. Acta Scientiarum. Agronomy, 34(2), 201-206. https://doi.org/10.4025/actasciagron.v34i2.13043

Singhania, A., & Singhania, P. K. (2014). Homoeopathy in agriculture. Building Organic Bridges 2, 667-70. https://doi.org/10.3220/REP_20_1_2014

Sutton, D. B. & Punja, Z. K. (2017). Investigating biospeckle laser analysis as a diagnostic method to assess sprouting damage in wheat seeds. Computers and Electronics in Agriculture 141, 238–47. https://doi.org/10.1016/j.compag.2017.07.027

Thomas, Y. (2015). From high dilutions to digital biology: the physical nature of the biological signal. Homeopathy 104, 295–300. https://doi.org/10.1016/j.homp.2015.06.008

Waisse, S., & Bonamin, L. B. (2019). Explanatory models for homeopathy part 2: state of art. Transdisciplinarity and Translationality in High Dilution Re- search. Signals and Images GIRI Series, Ed Leoni Bonamin & S. Waisse. Cambridge Scholars Publishing, 26-43.

Downloads

Published

14/02/2021

How to Cite

ZANCO, J. J. .; BOFF, P. .; WERNER, S. S. .; BOFF, M. I. C. . Biophototic in azuki bean seeds treated with ultrahigh dilutions . Research, Society and Development, [S. l.], v. 10, n. 2, p. e26110212462, 2021. DOI: 10.33448/rsd-v10i2.12462. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12462. Acesso em: 23 nov. 2024.

Issue

Section

Agrarian and Biological Sciences