Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13248

Keywords:

Cattle slaughter; Brucella spp.; Butcher; Zoonosis.

Abstract

Brucellosis is anthropozoonosis caused by Brucella spp. Among the zoonotic species, B. abortus is the main species affecting cattle and can easily be transmitted to humans. The purpose of this study was to investigate, through epidemiological inquiry and serological analysis,  animal and human health as related to smooth strain Brucella spp. in a slaughterhouse located in the southern region of the state of Bahia. For this purpose, blood samples were collected from workers and animals at a slaughterhouse together with the State Inspection Service. Then, the Buffered Acidified Plate Antigen test was performed for animals and humans, the Slow Agglutination Test was performed for humans only; and the Complement Fixation Test and the 2-Mercaptoethanol Brucella Agglutination test (2ME) were performed for animals only. In addition, an epidemiological inquiry was applied to workers in order to assess risk factors for the disease. After data analysis, it was concluded that infection by smooth strains of Brucella spp. was detected in 14.0% of the cattle. Additionally, one worker out 41 tested reactive to the disease.

Author Biographies

Lilia Marcia Paulin Silva, Instituto Biológico de São Paulo.

 

 

Nathana Kyolla Santos de Carvalho, Universidade Estadual de Santa Cruz

 

 

References

Alves, A. J. S., Gonçalves, V. S. P., Figueiredo, V. C. F. D., Lôbo, J. R., Bahiense, L., Amaku, M., ... & Dias, R. A. (2009). Situação epidemiológica da brucelose bovina no Estado da Bahia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61, 6-13. doi: https://doi.org/10.1590/S0102-09352009000700002

Antoniassi, N. A. B., Juffo, G. D., Pescadora, C. A., Corbellini, L. G., Sonne, L., Gomes, M. J. P., ... & Driemeier, D. (2016). Ocurrence and caracterization of bovine abortion caused by Brucella abortus infection in southern Brazil. Archivos de Medicina Veterinaria, 48(1), 43-49. doi: 10.4067/S0301-732X2016000100006

Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017a) Programa Nacional de Controle e Erradicação da Brucelose e da Tuberculose Animal (PNCEBT).

Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017b) IN 34, de 9 de setembro de 2017 Requisitos para diagnóstico de brucelose por laboratórios da Rede Mapa.

Brasil. Ministério da agricultura pecuária e abastecimento. (2017c) Decreto No 9.069, de 31 de março de 2017 dispõe sobre o regulamento da inspeção industrial e sanitária de produtos de origem animal (RIISPOA).

Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017d) Instrução Normativa SDA 10, de 3 de Março De 2017. Capítulo III que dispões acerca da Vacinação Contra a Brucelose.

Brasil. Ministério da Saúde. (2019) Secretaria de Vigilância em Saúde. Protocolo Estadual de Brucelose Humana: Manejo clinico e vigilância em saúde. Núcleo de Comunicação DIVE/SC.

Corbel, M. J. (2006). Brucellosis in humans and animals. World Health Organization. https://apps.who.int/iris/handle/10665/43597

de Macedo, A. A., Galvão, N. R., Sá, J. C., da Silva, A. P. D. C., da Silva Mol, J. P., Dos Santos, L. S., ... & de Carvalho Neta, A. V. (2019). Brucella-associated cervical bursitis in cattle. Tropical animal health and production, 51(3), 697-702. doi: https://doi.org/10.1007/s11250-018-1745-x

Emy, K. S., Misaco, W., Chusniati, S. & Maslachah, L. (2018) Isolation and identification of Brucella suis in pigs as zoonotic disease in endemic areas of east Java, Indonesia. African Journal of Infectious Diseases;12(1),148-51. doi: 10.2101/Ajid.12v1S.22

Foster, G., Osterman, B. S., Godfroid, J., Jacques, I., & Cloeckaert, A. (2007). Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. International journal of systematic and evolutionary microbiology, 57(11), 2688-2693. doi: 0.1099/ijs.0.65269-0

Hyeda, A., & Sbardellotto, F. Exposição acidental à vacina da brucelose. https://cdn.publisher.gn1.link/rbmt.org.br/pdf/v9n2a02.pdf

Jahans, K. L., Foster, G., & Broughton, E. S. (1997). The characterisation of Brucella strains isolated from marine mammals. Veterinary microbiology, 57(4), 373-382. doi: https://doi.org/10.1016/S0378-1135(97)00118-1

Kang, Y. S., Brown, D. A., & Kirby, J. E. (2019). Brucella neotomae recapitulates attributes of zoonotic human disease in a murine infection model. Infection and immunity, 87(1). doi: https://doi.org/10.1128/IAI.00255-18

Meirelles-Bartoli, R. B., & Mathias, L. A. (2010). Estudo comparativo entre os testes adotados pelo PNCEBT para o diagnóstico sorológico da brucelose em bovinos. Arquivos do Instituto Biológico, 77(1), 11-17. doi: https://doi.org/10.1590/1808-1657v77p0112010.

Mühldorfer, K., Wibbelt, G., Szentiks, C. A., Fischer, D., Scholz, H. C., Zschöck, M., & Eisenberg, T. (2017). The role of ‘atypical’Brucella in amphibians: are we facing novel emerging pathogens?. Journal of applied microbiology, 122(1), 40-53. doi: 10.1111/jam.13326

Osterman, B., & Moriyon, I. (2006). International committee on systematics of prokaryotes; subcommittee on the taxonomy of Brucella: minutes of the meeting, 17 September 2003, Pamplona, Spain. International journal of systematic and evolutionary microbiology, 56(5), 1173-1175. doi: https://doi.org/10.1099/ijs.0.64349-0

Paula, C. L. D., Mioni, M. D. S. R., Appolinário, C. M., Katayama, E. R., Allendorf, S. D., & Megid, J. (2015). Detection of Brucella spp. in unpasteurized cow milk by polymerase chain reaction (PCR). Arquivos do Instituto Biológico, 82. doi: http://dx.doi.org/10.1590/1808-1657000252013

Paulin, L. M., Prado, G. E. S., Federsoni, I. S. P., Teixeira, A. C., Castro, V., & Genovez, M. E. (2002). Estudo comparativo dos testes 2-mercaptoetanol e reação de fixação do complemento no sorodiagnóstico da brucelose bovina. Arquivos do Instituto Biológico, 69(4), 41-47. http://www.biologico.sp.gov.br/uploads/docs/arq/V69_4/paulin.pdf

Petry, A. C., Castro, B. G., & Freitas, F. (2019). Research of antibodies anti-Brucella abortus e anti-Brucella canis in dogs domiciled in dairy farms in the medium-north region of Mato Grosso. Scientific Electronic Archives, 12(2), 114-117. doi: https://doi.org/10.36560/1222019636

Ribeiro, A. C., McAllister, A. J., & Queiroz, S. A. D. (2003). Effect of culling reasons on lifetime profitability measures of dairy cows in Kentucky. Revista Brasileira de Zootecnia, 32(6), 1737-1746. doi: https://doi.org/10.1590/S1516-35982003000700025

Ribeiro, C. M., de Carvalho, J. L. B., de Santis Bastos, P. A., Mendes, R. G., Katagiri, S., & da Costa, V. M. (2020). Spatial and temporal trend analysis of bovine brucellosis in Brazil, 2014 to 2018. Semina: Ciências Agrárias, 41(4), 1279-1290. doi: http://dx.doi.org/10.5433/1679-0359.2020v41n4p1279

Rónai, Z., Kreizinger, Z., Dán, Á., Drees, K., Foster, J. T., Bányai, K., ... & Gyuranecz, M. (2015). First isolation and characterization of Brucella microti from wild boar. BMC veterinary research, 11(1), 1-6. doi: https://doi.org/10.1186/s12917-015-0456-z

Schneider, R. C., Santos, M. D., Lunardi, M., Benetti, A. H., Camargo, L. M., Freitas, S. H., ... & Costa, D. S. (2013). Prevalence of brucellosis and risk factors associated with its transmission to slaughterhouse employees in the Cuiaba metropolitan area in the state of Mato Grosso. Semina: Ciências Agrárias, 34(5), 2367-2373. doi: 10.5433/1679-0359.2013v34n5p2367

Scholz, H. C., Hofer, E., Vergnaud, G., Fleche, P. L., Whatmore, A. M., Dahouk, S. A., ... & Tomaso, H. (2009). Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Vector-borne and zoonotic diseases, 9(2), 153-156. doi: 10.1089/vbz.2008.0036

Scholz, H. C., Hubalek, Z., Sedláček, I., Vergnaud, G., Tomaso, H., Al Dahouk, S., ...& Nöckler, K. (2008). Brucella microti sp. nov., isolated from the common vole Microtus arvalis. International journal of systematic and evolutionary microbiology, 58(2), 375-382. doi: 10.1099/ijs.0.65356-0

Scholz, H. C., Nöckler, K., Göllner, C., Bahn, P., Vergnaud, G., Tomaso, H., ... & De, B. K. (2010). Brucella inopinata sp. nov., isolated from a breast implant infection. International journal of systematic and evolutionary microbiology, 60(4), 801-808. doi: 10.1099/ijs.0.011148-0

Skendros, P., & Boura, P. J. R. S. T. (2013). Immunity to brucellosis. Revue scientifique et technique (International Office of Epizootics), 32(1), 137-147. doi: 10.20506/rst.32.1.2190

Soares, C. D. P. O. C., Teles, J. A. A., Santos, A. F. D., Silva, S. O. F., Cruz, M. V. R. A., & Silva-Júnior, F. F. D. (2015). Prevalencia de la Brucella spp en humanos. Revista latino-americana de enfermagem, 23(5), 919-926. doi https://doi.org/10.1590/0104-1169.0350.2632.

Tortora, G. J., Funke, B. R., case, C.L. (2012). Microbiologia. 10ed. Porto alegre: artmed,. 934p.

Whatmore, A. M., Davison, N., Cloeckaert, A., Al Dahouk, S., Zygmunt, M. S., Brew, S. D., ... & Schlabritz-Loutsevitch, N. E. (2014). Brucella papionis sp. nov., isolated from baboons (Papio spp.). International journal of systematic and evolutionary microbiology, 64(Pt 12), 4120. doi: 10.1099/ijs.0.065482-0

Zygmunt, M. S., Jacques, I., Bernardet, N., & Cloeckaert, A. (2012). Lipopolysaccharide heterogeneity in the atypical group of novel emerging Brucella species. Clinical and Vaccine Immunology, 19(9), 1370-1373. doi: 10.1128/CVI.00300-12

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Published

20/03/2021

How to Cite

ALVES, T. M. .; MELO, P. de C. .; SILVA, L. M. P. .; CARVALHO, N. K. S. de .; RIOS , A. F. M. .; BARTOLI, R. B. M. .; OLIVEIRA JÚNIOR, C. A. de .; SANTOS , H. . F. . Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse. Research, Society and Development, [S. l.], v. 10, n. 3, p. e39710313248, 2021. DOI: 10.33448/rsd-v10i3.13248. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13248. Acesso em: 12 apr. 2021.

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Section

Agrarian and Biological Sciences