Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse
DOI:
https://doi.org/10.33448/rsd-v10i3.13248Keywords:
Cattle slaughter; Brucella spp.; Butcher; Zoonosis.Abstract
Brucellosis is anthropozoonosis caused by Brucella spp. Among the zoonotic species, B. abortus is the main species affecting cattle and can easily be transmitted to humans. The purpose of this study was to investigate, through epidemiological inquiry and serological analysis, animal and human health as related to smooth strain Brucella spp. in a slaughterhouse located in the southern region of the state of Bahia. For this purpose, blood samples were collected from workers and animals at a slaughterhouse together with the State Inspection Service. Then, the Buffered Acidified Plate Antigen test was performed for animals and humans, the Slow Agglutination Test was performed for humans only; and the Complement Fixation Test and the 2-Mercaptoethanol Brucella Agglutination test (2ME) were performed for animals only. In addition, an epidemiological inquiry was applied to workers in order to assess risk factors for the disease. After data analysis, it was concluded that infection by smooth strains of Brucella spp. was detected in 14.0% of the cattle. Additionally, one worker out 41 tested reactive to the disease.
References
Alves, A. J. S., Gonçalves, V. S. P., Figueiredo, V. C. F. D., Lôbo, J. R., Bahiense, L., Amaku, M., ... & Dias, R. A. (2009). Situação epidemiológica da brucelose bovina no Estado da Bahia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61, 6-13. doi: https://doi.org/10.1590/S0102-09352009000700002
Antoniassi, N. A. B., Juffo, G. D., Pescadora, C. A., Corbellini, L. G., Sonne, L., Gomes, M. J. P., ... & Driemeier, D. (2016). Ocurrence and caracterization of bovine abortion caused by Brucella abortus infection in southern Brazil. Archivos de Medicina Veterinaria, 48(1), 43-49. doi: 10.4067/S0301-732X2016000100006
Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017a) Programa Nacional de Controle e Erradicação da Brucelose e da Tuberculose Animal (PNCEBT).
Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017b) IN 34, de 9 de setembro de 2017 Requisitos para diagnóstico de brucelose por laboratórios da Rede Mapa.
Brasil. Ministério da agricultura pecuária e abastecimento. (2017c) Decreto No 9.069, de 31 de março de 2017 dispõe sobre o regulamento da inspeção industrial e sanitária de produtos de origem animal (RIISPOA).
Brasil. Ministério da Agricultura Pecuária e Abastecimento. (2017d) Instrução Normativa SDA 10, de 3 de Março De 2017. Capítulo III que dispões acerca da Vacinação Contra a Brucelose.
Brasil. Ministério da Saúde. (2019) Secretaria de Vigilância em Saúde. Protocolo Estadual de Brucelose Humana: Manejo clinico e vigilância em saúde. Núcleo de Comunicação DIVE/SC.
Corbel, M. J. (2006). Brucellosis in humans and animals. World Health Organization. https://apps.who.int/iris/handle/10665/43597
de Macedo, A. A., Galvão, N. R., Sá, J. C., da Silva, A. P. D. C., da Silva Mol, J. P., Dos Santos, L. S., ... & de Carvalho Neta, A. V. (2019). Brucella-associated cervical bursitis in cattle. Tropical animal health and production, 51(3), 697-702. doi: https://doi.org/10.1007/s11250-018-1745-x
Emy, K. S., Misaco, W., Chusniati, S. & Maslachah, L. (2018) Isolation and identification of Brucella suis in pigs as zoonotic disease in endemic areas of east Java, Indonesia. African Journal of Infectious Diseases;12(1),148-51. doi: 10.2101/Ajid.12v1S.22
Foster, G., Osterman, B. S., Godfroid, J., Jacques, I., & Cloeckaert, A. (2007). Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. International journal of systematic and evolutionary microbiology, 57(11), 2688-2693. doi: 0.1099/ijs.0.65269-0
Hyeda, A., & Sbardellotto, F. Exposição acidental à vacina da brucelose. https://cdn.publisher.gn1.link/rbmt.org.br/pdf/v9n2a02.pdf
Jahans, K. L., Foster, G., & Broughton, E. S. (1997). The characterisation of Brucella strains isolated from marine mammals. Veterinary microbiology, 57(4), 373-382. doi: https://doi.org/10.1016/S0378-1135(97)00118-1
Kang, Y. S., Brown, D. A., & Kirby, J. E. (2019). Brucella neotomae recapitulates attributes of zoonotic human disease in a murine infection model. Infection and immunity, 87(1). doi: https://doi.org/10.1128/IAI.00255-18
Meirelles-Bartoli, R. B., & Mathias, L. A. (2010). Estudo comparativo entre os testes adotados pelo PNCEBT para o diagnóstico sorológico da brucelose em bovinos. Arquivos do Instituto Biológico, 77(1), 11-17. doi: https://doi.org/10.1590/1808-1657v77p0112010.
Mühldorfer, K., Wibbelt, G., Szentiks, C. A., Fischer, D., Scholz, H. C., Zschöck, M., & Eisenberg, T. (2017). The role of ‘atypical’Brucella in amphibians: are we facing novel emerging pathogens?. Journal of applied microbiology, 122(1), 40-53. doi: 10.1111/jam.13326
Osterman, B., & Moriyon, I. (2006). International committee on systematics of prokaryotes; subcommittee on the taxonomy of Brucella: minutes of the meeting, 17 September 2003, Pamplona, Spain. International journal of systematic and evolutionary microbiology, 56(5), 1173-1175. doi: https://doi.org/10.1099/ijs.0.64349-0
Paula, C. L. D., Mioni, M. D. S. R., Appolinário, C. M., Katayama, E. R., Allendorf, S. D., & Megid, J. (2015). Detection of Brucella spp. in unpasteurized cow milk by polymerase chain reaction (PCR). Arquivos do Instituto Biológico, 82. doi: http://dx.doi.org/10.1590/1808-1657000252013
Paulin, L. M., Prado, G. E. S., Federsoni, I. S. P., Teixeira, A. C., Castro, V., & Genovez, M. E. (2002). Estudo comparativo dos testes 2-mercaptoetanol e reação de fixação do complemento no sorodiagnóstico da brucelose bovina. Arquivos do Instituto Biológico, 69(4), 41-47. http://www.biologico.sp.gov.br/uploads/docs/arq/V69_4/paulin.pdf
Petry, A. C., Castro, B. G., & Freitas, F. (2019). Research of antibodies anti-Brucella abortus e anti-Brucella canis in dogs domiciled in dairy farms in the medium-north region of Mato Grosso. Scientific Electronic Archives, 12(2), 114-117. doi: https://doi.org/10.36560/1222019636
Ribeiro, A. C., McAllister, A. J., & Queiroz, S. A. D. (2003). Effect of culling reasons on lifetime profitability measures of dairy cows in Kentucky. Revista Brasileira de Zootecnia, 32(6), 1737-1746. doi: https://doi.org/10.1590/S1516-35982003000700025
Ribeiro, C. M., de Carvalho, J. L. B., de Santis Bastos, P. A., Mendes, R. G., Katagiri, S., & da Costa, V. M. (2020). Spatial and temporal trend analysis of bovine brucellosis in Brazil, 2014 to 2018. Semina: Ciências Agrárias, 41(4), 1279-1290. doi: http://dx.doi.org/10.5433/1679-0359.2020v41n4p1279
Rónai, Z., Kreizinger, Z., Dán, Á., Drees, K., Foster, J. T., Bányai, K., ... & Gyuranecz, M. (2015). First isolation and characterization of Brucella microti from wild boar. BMC veterinary research, 11(1), 1-6. doi: https://doi.org/10.1186/s12917-015-0456-z
Schneider, R. C., Santos, M. D., Lunardi, M., Benetti, A. H., Camargo, L. M., Freitas, S. H., ... & Costa, D. S. (2013). Prevalence of brucellosis and risk factors associated with its transmission to slaughterhouse employees in the Cuiaba metropolitan area in the state of Mato Grosso. Semina: Ciências Agrárias, 34(5), 2367-2373. doi: 10.5433/1679-0359.2013v34n5p2367
Scholz, H. C., Hofer, E., Vergnaud, G., Fleche, P. L., Whatmore, A. M., Dahouk, S. A., ... & Tomaso, H. (2009). Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Vector-borne and zoonotic diseases, 9(2), 153-156. doi: 10.1089/vbz.2008.0036
Scholz, H. C., Hubalek, Z., Sedláček, I., Vergnaud, G., Tomaso, H., Al Dahouk, S., ...& Nöckler, K. (2008). Brucella microti sp. nov., isolated from the common vole Microtus arvalis. International journal of systematic and evolutionary microbiology, 58(2), 375-382. doi: 10.1099/ijs.0.65356-0
Scholz, H. C., Nöckler, K., Göllner, C., Bahn, P., Vergnaud, G., Tomaso, H., ... & De, B. K. (2010). Brucella inopinata sp. nov., isolated from a breast implant infection. International journal of systematic and evolutionary microbiology, 60(4), 801-808. doi: 10.1099/ijs.0.011148-0
Skendros, P., & Boura, P. J. R. S. T. (2013). Immunity to brucellosis. Revue scientifique et technique (International Office of Epizootics), 32(1), 137-147. doi: 10.20506/rst.32.1.2190
Soares, C. D. P. O. C., Teles, J. A. A., Santos, A. F. D., Silva, S. O. F., Cruz, M. V. R. A., & Silva-Júnior, F. F. D. (2015). Prevalencia de la Brucella spp en humanos. Revista latino-americana de enfermagem, 23(5), 919-926. doi https://doi.org/10.1590/0104-1169.0350.2632.
Tortora, G. J., Funke, B. R., case, C.L. (2012). Microbiologia. 10ed. Porto alegre: artmed,. 934p.
Whatmore, A. M., Davison, N., Cloeckaert, A., Al Dahouk, S., Zygmunt, M. S., Brew, S. D., ... & Schlabritz-Loutsevitch, N. E. (2014). Brucella papionis sp. nov., isolated from baboons (Papio spp.). International journal of systematic and evolutionary microbiology, 64(Pt 12), 4120. doi: 10.1099/ijs.0.065482-0
Zygmunt, M. S., Jacques, I., Bernardet, N., & Cloeckaert, A. (2012). Lipopolysaccharide heterogeneity in the atypical group of novel emerging Brucella species. Clinical and Vaccine Immunology, 19(9), 1370-1373. doi: 10.1128/CVI.00300-12
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Thaise Marques Alves; Poliana de Castro Melo; Lilia Marcia Paulin Silva; Nathana Kyolla Santos de Carvalho; Amora Ferreira Menezes Rios ; Raphaella Barbosa Meirelles Bartoli; Carlos Augusto de Oliveira Júnior; Henrique Fernando Santos
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
