Morphoanatomical study, seasonal variation, and larvicidal activity of volatile oils from the leaves of Campomanesia pubescens (DC.) O. Berg (Myrtaceae)

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13412

Keywords:

Plant anatomy; Cerrado; Essential oil; Medicinal plants; Larvicidal activity.

Abstract

The aim of this study was to perform from the morpho-anatomical study, physicochemical characterization, chemical composition, seasonal variability and larvicidal activity of the volatile oils of Campomanesia pubescens (DC.) O. Berg leaves. The botanical material was collected in Hidrolândia, Goiás. Morpho-anatomical characterization and phytochemical screening were performed by conventional methods. Leaf powders were submitted to hydrodistillation in the Clevenger apparatus and the identification of the chemical components of the volatile oils obtained was done by GC-MS. The larvicidal activity was carried out with third-stage larvae of the Aedes aegypti. The leaf blade, the main vein, and the young stem have a uni-stratified epidermis covered by a cuticle. The mesophyll and cortical parenchyma of the main vein and young stem have secretory cavities and idioblasts with crystals. The main rib contains a bicolateral bundle in an open arch. The young stem has a discontinuous band of sclerenchyma external to the phloem cells. Most volatile oil compounds are spathulenol, caryophyllene oxide, α-macrocarpene, and z-caryophylleneIn phytochemical analysis, tannins, digitalis, flavonoids, and total phenols were detected. The content of volatile compounds was 7.36%, that of total ash was 1.77%, and that of mucilage was 3.52 ml. The volatile oil at the concentration used was inactive against Ae aegypti larvae. The present study contributes to the taxonomic knowledge of the species and provides parameters for quality control of the plant raw material. This work represents the first description of the chemical compounds and seasonal variability of volatile oils from C. pubescens leaves collected in Goiás state.

References

Abe, S. Y., Mendes Silva, S., Possamai, J. C. & Nakashima, T. (2014). Prospecção fitoquímica, teor de flavonoides totais e capacidade antioxidante de Campomanesia xanthocarpa Mart. ex O. Berg (Myrtaceae). Revista Eletrônica de Farmácia, 11 (2), 1-14.

Adams, R. P. (2007). Identification of essential oil components by Gas Chromatography/Mass Spectroscopy. (4th ed.), Carol Stream, IL: Allured Publ. Corp.

Almeida, S. P., Proença, C. E. B., Sano, S. M. & Ribeiro, J. F. (1998). Cerrado: espécies vegetais úteis. Planaltina: EMBRAPA-CPAC – DF.

Amaral, É. V. E. J., Reis, E. F., Ressel, K., & Pinto F. N. (2016). Descrição morfológica de duas espécies de Campomanesia ruiz & pavon (Myrtaceae). Revista Agrotecnologia, 7(1), 42-52.

Amer, A. & Mehlhorn, H. (2006). Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitology Research, 99 (4), 466-472. https://doi.org/10.1007/s00436-006-0182-3

An, N. T. G, Huong, L. T., Satyal, P., Tai, T. A., Dai, D. N., Hung, N. H., Ngoc, N. T. B. & Setzer, W. N. (2020). Mosquito larvicidal activity, antimicrobial activity, and chemical compositions of essential oils from four species of Myrtaceae from Central Vietnam. Plants, 22, 9(4), 544. https://doi: 10.3390/plants9040544.

Appezzato-da-Glória, B. & Carmello-Guerreiro, S. M. (2009). Anatomia vegetal. Editora UFV.

APG IV (2016). Angiosperm Phylogeny Website. Version 14. Missouri Botanical Garden Retrieved from http://www.mobot.org/MOBOT/research/APweb/

Brasil (1988). Farmacopéia Brasileira. (4th ed.), Atheneu.

Brasil. Ministério da Saúde (2010). ANVISA. RDCNº10, de 9 de março de 2010. Dispõe sobre a notificação de drogas vegetais junto à ANVISA e dá outras providências. http://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2010/res0010_09_03_2010.html.

Cardoso C. A. L. & R-Poppi, N. (2009). Identification of the volatile compounds of flower oil of Campomanesia pubescens (Myrtaceae). Journal of Essential Oil Research, 21 (5), 433-434. https://doi.org/10.1080/10412905.2009.9700210

Cardoso, A. C. L., Salmazzo, G. R., Honda, N. K., Prates C. B., Vieira, M. C. & Coelho, R. G. (2010). Antimicrobial activity of the extracts and fractions of hexanic fruits of Campomanesia Species (Myrtaceae). Journal of Medical Food, 13 (5), 1273–1276. https://doi.org/10.1089/jmf.2009.0047

Carr, D. J. & Carr, S. M. G. (1970). Oil glands and ducts in Eucalyptus L´Hérit. II. Development and structure of oil glands in the embryo. Australian Journal of Botany, 18 (2), 191-212. https://doi.org/10.1071/BT9700191

Castro, M. M. & Machado, S. R. (2003). Células e tecidos secretores. In: Appezzato-da-Glória B, Carmello-Guerreiro SM (eds). Anatomia Vegetal. Viçosa: Universidade Federal de Viçosa.

Chang, R., Morais, S. A. L., Nascimento, E. A., Cunha, L. C. S., Rocha, E.O., Aquino, F. J. T., Souza, M. G. M., Cunha W. R. & Martins C. H. G. (2011). Essential oil composition and antioxidant and antimicrobial properties of Campomanesia pubescens O. Berg, native of brazilian Cerrado. Latin American Journal of Pharmacy, 30 (9): 1843-1848.

Consoli, R. A. G. B. & Oliveira, R. L. (1994). Principais mosquitos de importância sanitária no Brasil. Editora Fiocruz.

Conti, E, Litt, A., Wilson, P. G., Graham, S. A., Briggs, B. G., Johnson, L. A. S. & Sytsma, K. J. (1997). Interfamilial relationships in Myrtales: molecular phylogeny and patterns of morphological evolution. Systematic Botany, 22 (4), 629-647. https://doi.org/10.2307/2419432

Costa, A. F. (2001). Farmacognosia. Fundação Calouste Gulbenkian.

Costa, J. G. M., Rodrigues, F. F. G., Angélico, E. C., Silva, M. R., Mota, M. L., Santos, N. K. A., Cardoso, A. L. H. & Lemos, T. L. G. (2005). Chemical biological study of the essential oils of Hyptis martiusii, Lippia sidoides and Syzigium aromaticum against larvae of Aedes aegypti and Culex quinquefasciatus. Brazilian Journal of Pharmacognosy, 15, 304–309. https://doi.org/10.1590/S0102-695X2005000400008

Cunha, A. P. (2005). Farmacognosia e fitoquímica. Fundação Caloustre Gulbenkian.

Dias, C. N. & Moraes, D. F. C. (2014). Essential oils and their compounds as Aedes aegypti L. (Diptera: Culicidae) larvicides: review. Parasitology Research, 113, 565–592. https://doi.org/10.1007/s00436-013-3687-6.

Fahn, A. (1979). Secretory tissues in plants. Academic Press.

Fidalgo, A. O. & Kleinert, A. M. P. (2009). Reproductive biology of six Brazilian Myrtaceae: is there a syndrome associated with buzz-pollination? New Zealand Journal of Botany, 47 (4), 355–365. https://doi.org/10.1080/0028825x.2009.9672712

Forzza, R. C. (2010). Catálogo de plantas e fungos do Brasil. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro.

Gentry, A. H. (1992). Bignoniaceae Part II: Tribe Tecomeae. Flora Neotropica, 25 (2), 51-105.

Gobbo-Neto, L. & Lopes, N. P. (2007). Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Química Nova, 30 (2), 374-381. http://dx.doi.org/10.1590/S0100-40422007000200026

Gomes, S. M., Somavilla, N. S. D. N., Gomes-Bezerra, K. M., Miranda, S. C., Carvalho, P. S. & Graciano-Ribeiro, D. (2009). Anatomia foliar de espécies de Myrtaceae: contribuições à taxonomia e filogenia. Acta Botanica Brasilica, 23, 224–238. https://doi.org/10.1590/S0102-33062009000100024

Guerrero, F. M. G., Zimmerman, L. R., Cardoso, E. V., Lima, C. A., Cardoso de Perdomo, R. T., Alva, R. & Carollo, C. A. (2010). Investigação da toxicidade crônica das folhas de guavira (Campomanesia pubescens) em ratos machos. Revista Fitos, 5 (2), 64-72.

INMET (2020). Instituto Nacional de Meteorologia. Ministério da agricultura, pecuária e abastecimento. http://www.inmet.gov.br/portal/ (acessado em novembro de 2020).

Isman, M. B. (2020). Botanical insecticides in the twenty-first century-fulfilling their promise? Annual Review of Entomology, 65, 233–249. https://doi.org/10.1146/annurev-ento-011019-025010

Katsube, N., Iwashita, K., Tsushida, T., Yamaki, K. & Kobori, M. (2003). Induction of apoptosis in cancer cells by bilberry (Vaccinium mirtillus) and the anthocyanins. Journal of Agricultural and Food Chemistry, 51, 68-75. https://doi.org/10.1021/jf025781x

Kraus, J. E. & Arduin, M. (1997). Manual básico de métodos em morfologia vegetal. Ed. Universidade Rural.

Kuster, V. C. & Vale, F. H. A. (2016). Leaf histochemistry analysis of four medicinal species from Cerrado. Brazilian Journal of Pharmacognosy, 26, 673–678. http://dx.doi.org/10.1016/j.bjp.2016.05.015

Landrum, L. R. (1986). Campomanesia, Pimenta, Blepharocalyx, Legrandia, Acca, Myrrhinium, and Luma (Myrtaceae). Flora Neotropica.

Landrum, L. R. & Kawasaki, M. L. (1997). The genera of Myrtaceae in Brazil: an illustrated synoptic treatment and identification keys. Brittonia, 49, 508-536. https://doi.org/10.2307/2807742

Leyva, M., Marquetti, M. C., Tacoronte, J. E., Scull, R., Tiomno, O., Mesa, A. & Montada, D. (2009). Actividad larvicida de aceites esenciales de plantas contra Aedes aegypti (L.) (Diptera: Culicidae). Revista Biomed, 20, 5–13.

Lima, D. F., Goldenberg, R. & Sobral, M. (2011a). O gênero Campomanesia (Myrtaceae) no estado do Paraná, Rodriguésia, 62, 683-693. https://doi.org/10.1590/2175-7860201162313

Lima, M. A. A., Oliveira, F. F. M., Gomes, G. A. et al. (2011b). Evaluation of larvicidal activity of the essential oils of plants species from Brazil against Aedes aegypti (Diptera: Culicidae). African Journal of Biotechnology, 10, 11716–11720. https://doi.org/10.5897/AJB11.1102

Lorenzi, H., Souza, H. M., Torres, M. A. V. & Bacher, L. B. (2003). Árvores exóticas do Brasil: madeireiras, ornamentais e aromaticas. Nova Odessa: Instituto Plantarium de Estudos da Flora.

Lucia, A., Licastro, S., Zerba, E. & Masuh, H. (2008). Yield, chemical composition, and bioactivity of essential oils from 12 species of Eucalyptus on Aedes aegypti larvae. The Netherlands Entomological Society, 129, 107–114. https://doi.org/10.1111/j.1570-7458.2008.00757.x

Martius, C. F. P. (1897). Flora Brasiliensis, enumeratio plantarum in Brasilia hactenus detectarum: quas suis aliorumque botanicorum studiis descriptas et methodo naturali digestas partim icone illustratas. Flora Brasiliensis, 8(2), 383.

Metcalfe, C. R. & Chalk, L. (1950). Anatomy of the dicotyledons. Oxford University Press.

Muturi, E. J., Selling, G. W., Doll, K. M., Hay, W. T. & Ramirez, J. L. (2020). Leptospermum scoparium essential oil is a promising source of mosquito larvicide and its toxicity is enhanced by a biobased emulsifier. PLoS ONE, 15(2), 1-14. https://doi.org/10.1371/journal.pone.0229076

Ness, A. R. & Powles, J. W. (1997). Fruit and vegetables, and cardiovascular disease: a review. International Journal of Epidemiology, 26 (1), 1-13. https://doi.org/10.1093/ije/26.1.1

Nucci, M. & Alves-Junior, V. V. (2017). Biologia floral e sistema reprodutivo de Campomanesia adamantium (Cambess.) O. Berg- Myrtaceae em área de Cerrado no sul do Mato Grosso do Sul, Brasil. Interciencia, 42 (2), 127-131.

Oliveira, J. D., Alves, C. C. F., Miranda, M. L. D., Martins, C. H. G., Silva, T. S., Ambrosio M. A. L. V., Alves, J. M. & Silva, J. P. (2016). Rendimento, composição química e atividades antimicrobiana e antioxidante do óleo essencial de folhas de Campomanesia adamantium submetidas a diferentes métodos de secagem. Revista Brasileira de Plantas Medicinais, 18, 502- 510. https://doi.org/10.1590/1983-084X/15_206

Oliveira, M. I. U., Rebouças, D. A., Leite, K. R. B., Oliveira, R. P.& Funch, L. S. (2018). Can leaf morphology and anatomy contribute to species delimitation? A case in the Campomanesia xanthocarpa complex (Myrtaceae), Flora, 249, 111-123. https://doi.org/10.1016/j.flora.2018.10.004

Rodrigues, V. E. G. & Carvalho, D. A.(2001). Plantas medicinais no domínio dos cerrados. UFLA.

Sangwan, N. S., Farooqi, A. H. A., Shabih, F. & Sangwan, R. S. (2001). Regulation of essential oil production in plant. Plant Growth Regulation, 34, 3-21. https://doi.org/10.1023/A:1013386921596

Sano, S. M. & Almeida, S. P. (1998). Cerrado: Ambiente e Flora. Mendonça RC et al. Flora vascular do Cerrado. Embrapa CPAC.

Schmid, R. (1972). A resolution of the Eugenia-Syzygium controversy (Myrtaceae). American Journal of Botany, 59, 423-436. https://doi.org/10.2307/2441553

Silva, E. P., Vilas Boas, E. V. B., Rodrigues, L. J. & Siqueira, H. H. (2009). Physical, chemical and physiological characterization of gabiroba fruit. (Campomanesia pubescens) during its development. Ciência e Tecnologia de Alimentos, 29, 4, 803-809. http://dx.doi.org/10.1590/S0101-20612009000400016.

Silva, I. G., Silva, H. H. G. & Lima, C. G. (2003). Ovipositional behavior of Aedes aegypti (Diptera, Culicidae) in different strata and biological cycle. Acta Biológica Paranaense, 32:1-8.

Silvério, M. R. S., Espindola, L. S., Lopes, N. P. & Vieira, P. C. (2020). Plant natural products for the control of Aedes aegypti: The main vector of important arboviruses. Molecules, 25(15), 3484:1-42. https://doi.org/10.3390/molecules25153484

Sobral, M., Proença, C., Souza, M., Mazine, F. & Lucas, E. (2015). Myrtaceae in lista de espécies da flora do Brasil. Jardim Botânico do Rio de Janeiro. Retrieved from http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB171

Stoclet, J. C., Chataigneau, T., Ndiaye, M., Oak, M., Bedoui, J. E., Chataigneau, M. & Schini-Kerth, V. B. (2004). Vascular protection by dietary polyphenols. European Journal of Pharmacology, 500, 299-313. https://doi.org/10.1016/j.ejphar.2004.07.034

Van Den Dool, H. & Kratz, P. D. (1963). A generalization of the Retention Index system including linear temperature programmed gas – liquid partition chromatography. Journal of Chromatography, 11, 463–471. https://doi.org/10.1016/S0021-9673(01)80947-X

Viana, G. A., Sampaio, C. G. & Martins, V. E. P. (2018). Produtos naturais de origem vegetal como ferramentas alternativas para o controle larvário de Aedes aegypti e Aedes albopictus. Journal of Health & Biological Sciences, 6(4), 449-462. https://doi.org/10.12662/2317-3076jhbs.v6i4.2079.

Wang, J. & Mazza, G. (2002). Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor alpha in LPS/IFN-gamma-activated RAW 264.7 macrophages. Journal of Agricultural and Food Chemistry, 50, 4183-4189. https://doi.org/10.1021/jf011613d

Ward, J. H. (1963). Hierarchical grouping to optimize an objective function. Journal of the American Statistical Association, 58, 236-244. https://doi.org/10.1080/01621459.1963.10500845

World Health Organization (2005). Guidelines for laboratory and field testing of mosquito larvicides. World Health Organization. Retrieved from http://apps.who.int/iris/bitstream/10665/69101/1/WHO_CDS_WHOPES_GCDPP_2005.13.pdf.

Zara, A. L. S. A., Santos, S. M., Fernandes-Oliveira, E. S, Carvalho, R. G. & Coelho, G. E. (2016). Estratégias de controle do Aedes aegypti: uma revisão. Epidemiologia e Serviços de Saúde, 25(2), 391–404. https://doi.org/10.5123/S1679-4974201600020001

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18/03/2021

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COSTA, H. M. da .; FIUZA, T. de S.; FERREIRA, H. D. .; ABRAO, F. Y. .; ROMANO, C. A. .; BORGES, L. L. .; PAULA, J. R. de . Morphoanatomical study, seasonal variation, and larvicidal activity of volatile oils from the leaves of Campomanesia pubescens (DC.) O. Berg (Myrtaceae). Research, Society and Development, [S. l.], v. 10, n. 3, p. e35610313412, 2021. DOI: 10.33448/rsd-v10i3.13412. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13412. Acesso em: 22 nov. 2024.

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Health Sciences