Phytochemical, physicochemical, microbiological study and anticholinesterase activity of Ginkgo biloba L. and Bacopa monnieri L. used in phytotherapy

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13480

Keywords:

Quality control; Phytochemical analysis; Anticholinesterase activity; Ginkgo biloba L.; Bacopa monnieri L.

Abstract

Herbal medicines based on Ginkgo biloba L. and Bacopa monnieri L. are used to improve memory and cognitive function. The quality control and anticholinesterase activity of the herbal medicines prepared from G. biloba and B. monnieri were evaluated which are commercialized in handling pharmacies. Samples of herbal medicines based on G. biloba and B. monnieri were obtained from handling pharmacies in Teresina-PI, submitted to labeling analysis and anticholinesterase activity. The phytochemical study was performed by preliminary prospecting, TLC and HPLC. The tests physicochemical and microbiological analyses were made according to the Pharmacopoeia 2010. It was observed that the labeling, foreign material, disintegration, and microbiological parameters were in accordance with ANVISA standards. The colorimetric tests were uniform in the samples of B. monnieri and variable of G. biloba. The average weight evaluation shows that the G1 and B3 samples have capsular content above that described in the labeling and presented pH values ​​different. Among the thermogravimetric profiles, the mass losses of samples G1 and B3 showed values ​​with high ash content, suggesting adulterations. Phytochemical prospecting showed flavonoids as common secondary metabolites in both species, corroborating with TLC and HPLC analysis, which identified the compounds chlorogenic acid, rutin, myricetin and quercetin. The samples G4, B2 and B5 present metabolites capable of inhibiting the enzyme acetylcholinesterase with IC50 of 0.8540 mg/mL, 0.9650 mg/mL and 1.8221×10-5 mg/mL, respectively. The samples G1 and B3 of G. biloba and B. monnieri, did not obey some parameters of quality control for herbal medicines according to the criteria of the Brazilian Pharmacopeia.

Author Biographies

Tatiana de Oliveira Lopes, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Jaqueline Fernanda de Sousa Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Rudielson dos Santos Silva, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renata da Silva Carneiro, Piaui State University

Departamento de Química, PPGQ -GERATEC- Universidade Estadual do Piauí, Teresina, PI, CEP-64002-150, Brasil.

Antônio Rafael de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Yáscara Lopes de Oliveira, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Renato Pinto de Sousa, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil

Clara Andrezza Crisóstomo Bezerra Costa, Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

 

Iron Jonhson de Araújo Veras, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil

Ronaldo dos Santos Sousa Júnior, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Johnnatan Duarte de Freitas , Alagoas Federal Institute

Chemistry Department - Alagoas Federal Institute, Maceió, AL, CEP-57035-350, Brazil

Mahendra Rai, SGB Amravati University

Department of Biotechnology, SGB Amravati University, Amravati, 444 602, Maharashtra State, India

Laécio Santos Cavalcante, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Francisca Lucia de Lima, Piaui State University

Biology Department, LABMICRO, Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

Mariana Helena Chaves, Piaui Federal Universit

Chemistry Department, LPN, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Chistiane Mendes Feitosa, Piaui Federal Universit

Pharmacy Department, LPNNEX, Piaui Federal Universit, CEP - 64049-550 Teresina, PI, Brazil.

Valdiléia Teixeira Uchôa, Piaui State University

Chemistry Department, PPGQ -GERATEC- Piaui State University, Teresina, PI, CEP-64002-150, Brazil.

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20/03/2021

How to Cite

LOPES, T. de O. .; SILVA, J. F. de S. .; SILVA, R. dos S. .; CARNEIRO, R. da S. .; OLIVEIRA, A. R. de .; OLIVEIRA, Y. L. de .; SOUSA, R. P. de .; COSTA, C. A. C. B. .; VERAS, I. J. de A. .; SOUSA JÚNIOR, R. dos S. .; FREITAS , J. D. de .; RAI, M.; CAVALCANTE, L. S. .; LIMA, F. L. de .; CHAVES, M. H. .; FEITOSA, C. M. .; UCHÔA, V. T. . Phytochemical, physicochemical, microbiological study and anticholinesterase activity of Ginkgo biloba L. and Bacopa monnieri L. used in phytotherapy. Research, Society and Development, [S. l.], v. 10, n. 3, p. e39010313480, 2021. DOI: 10.33448/rsd-v10i3.13480. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13480. Acesso em: 12 apr. 2021.

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Health Sciences