Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients

Authors

DOI:

https://doi.org/10.33448/rsd-v10i4.13842

Keywords:

Micronuclei chromossome-defective; Pathology; Oral mucosa; Tobacco use disorder.

Abstract

Exfoliative cytology screening for genotoxicity is an important strategy for mitigating tobacco-induced carcinogenesis. The aim of this study was to determine the frequency of micronuclei in oral epithelial cells of smoking and non-smoking patients. Sixteen volunteers, of both sexes, 40-60 years old, with healthy oral mucosa were divided into smoking or non-smoking group. Cytological smears of buccal mucosa, lateral border of the tongue and floor of the mouth stained by Pap stain allowed evaluation of 4800 microscopic images. Frequencies of micronucleated cells (MNC) and micronuclei/cell (MN/C) were compared by group, anatomical region, sex and age range, using the two-way ANOVA test followed by Tukey (p<0.05). There was a greater presence of MNC in the smoker than in the non-smoker group, with significant differences for floor of mouth, female gender and age range of 50-60 years. The amount of 1 or 2 MN/C was greater than 3 or ≥4 MN/C, especially in the smoking group. There were differences in the frequency of MN/C between smokers and non-smokers, for the three oral regions, for both sexes although more accentuated in women, and for the age range of 50-60 years beyond the age range of 40-50 years for the non-smoking group. Differences in MNC and MN/C according to oral regions, sex and age between smokers and non-smokers contribute to understand the genotoxicity mediated by tobacco.

Author Biographies

Haydee Gina Quispe Jiménez, Universidade Federal do Ceará

Mestra pelo Programa de Pós-graduação em Biotecnologia da Universidade Federal do Ceará (Campus de Sobral).

Rômulo Melo Mesquita, Universidade Federal do Ceará

Mestrando pelo Programa de Pós-graduação em Biotecnologia da Universidade Federal do Ceará (Campus de Sobral).

Victor Bento Oliveira, Universidade Federal do Ceará

Mestrando pelo Programa de Pós-graduação em Odontologia da Universidade Federal do Ceará (Campus de Fortaleza).

Ana Larissa de Queiroz França, Universidade Federal do Ceará

Mestra pelo Programa de Pós-graduação em Ciências da Saúde da Universidade Federal do Ceará (Campus de Sobral).

Marcelo Miranda de Melo, Universidade Federal do Ceará

Professor Colaborador do Programa de Pós-graduação em Biotecnologia da Universidade Federal do Ceará (Campus de Sobral).

Ana Liza Paz Sousa Batista, Universidade Federal do Ceará

Professora Visitante do Programa de Pós-graduação em Biotecnologia da Universidade Federal do Ceará (Campus de Sobral).

Igor Iuco Castro-Silva, Universidade Federal do Ceará

Professor Permanente do Programa de Pós-graduação em Biotecnologia e do Curso de Bacharelado em Odontologia, Coordenador do Laboratório de Biomateriais da Universidade Federal do Ceará (Campus de Sobral).

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Published

01/04/2021

How to Cite

QUISPE JIMÉNEZ, H. G. .; MESQUITA, R. M. .; OLIVEIRA, V. B. .; FRANÇA, A. L. de Q. .; MELO, M. M. de .; BATISTA, A. L. P. S. .; CASTRO-SILVA, I. I. Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients. Research, Society and Development, [S. l.], v. 10, n. 4, p. e8810413842, 2021. DOI: 10.33448/rsd-v10i4.13842. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13842. Acesso em: 20 apr. 2021.

Issue

Section

Health Sciences