In vitro antitumoral activity of Prosopis juliflora in front of melanoma type cancerogen cells
DOI:
https://doi.org/10.33448/rsd-v10i4.14095Keywords:
Antitumor activity; Melanoma; Natural products; Prosopis juliflora.Abstract
The aim of this study was to investigate the antitumor activity of Prosopis juliflora against human tumor cells of the B16-F10 lineage (melanoma) to search for new drugs derived from natural sources. In vitro tests were conducted with the crude extracts of leaves and stem bark against the tumor cell lines B16-F10 through the cytotoxicity test using MTT (3- (4,5-dimethiazol-2yl) 2,5-diphenyl tetrazolium of bromide) by monitoring after 24 and 48 h of exposure of tumor and non-tumor cells to different concentrations of the extracts (100; 50; 25; 12.5 μg/mL). The cytotoxicity tests were performed using non-tumor cells (macrophages lineage J744). The stem bark extract and the leaf extract of P. juliflora inhibited the proliferation of B16-F10 cells from the lowest tested concentration 12.5 Mg / ml and did not show cytotoxicity against non-tumor cells. However, P. juliflora leaf extract showed a considerable decrease in antineoplastic activity from 50 mg / ml. It is suggested the chemical study for the extraction and isolation of active compounds from the extract of the leaves and stem bark of P. juliflora, and through testing these compounds in vitro and in vivo to investigate their possible antitumor activity.
References
Amaria, N. R., Menzie, A. M., Burton, E. M., Scolyer, R. A., Tetzlaff, M. T., Antdbacka, R., Ariyan, C., Bassett, R., Carter, B., Daud, A., Faries, M., Fecher, L. A., Flaherty, K. T., Gershenwald, J. E., Hamid, O., Hong, A., Kirkwood, J. M., Lo, S., Margolin, K., Messina, J., Michael, A. P., M. A., Rizos, H., Ross, M. I., Saw, E. A. R. R. P.M., Sondak, V., Sullivan, R. J., Taube, J. M., Thompson, J. F., Wiel, B. A. V. W. & Eggermont, A. M. (2019). Neoadjuvant systemic therapy in melanoma:recommendations of the International Neoadjuvant Melanoma Consortium. Lancet Oncology. 20, 7, 378-389.
Aumeeruddy, M. Z., & Mahomoodally, M. F. (2021). Global documentation of traditionally used medicinal plants in cancer management: A systematic review. South African Journal of Botany, 138, 5, 424-494.
Brasil. Ministério Da Saúde. Instituto Nacional De Câncer José Alencar Gomes Da Silva (2020). Coordenação de Prevenção e Vigilância. Estimativa 2020: incidência de câncer no Brasil. INCA.
Carvalho, N. C. (2013). Avaliação da atividade antineolpásica do extrato etanólico da própolis G6 baiana. Dissertação de Mestrado, Centro de Pesquisas Gonçalo Moniz, Fiocruz, Salvador, BA, Brasil.
Costa, A. C. F. & Cavalcante, G. M. (2018). Atividade antitumoral in vitro de Prosopis juliflora frente a células tumorais de câncer de mama e câncer de ovário. Acta Biomedica Basiliensia, 9,1, 131-135.
Dent, M. & Matoba, N. (2020). Cancer biologics made in plants. Current Opinion in Biotechnology, 61, 2, 82-88.
Henciya, S., Seturaman, P., James, A. R., Tsai, Y. H., Nikam, R., Wu, Y. C., Dahms, H. U. & Chang, F. R. (2016). Biopharmaceutical potentials of Prosopis spp. (Mimosaceae,Legume). Journal of Food and Drug Analysis, 30, 1, 1-10.
Ibrahim, M., Nadir, M. & Ali, A. (2013). Phytochemical analyses of Prosopis juliflora Swartz D.C. Pakistan Journal of Botany, 45, 2, 20101-2104.
Joung, J. H., Hwang, J., Kim, J. H., Sim, Deok, Y. Si., Im, E., Parque, J. E, Parque, W. Y., Shim, B. S., Kim, B. & Kim, S. H. (2021). Phyotochemical candidates repurposing for câncer therapy and their molecular. Seminars in Cancer Biology, 68, 1, 164-174.
Kuruppu, A. L., Paranagama, P. & Goonasekara, C. (2019). Medicinal plants commonly used against cancer in traditional medicine formula in Sri Lanka. Saudi Pharmaceutical Jornal. 27, 4, 565-573.
Malik, S. K., Ahmed, M. & Khan, F. (2018). Identification of new anticancer terpenoids from Prosopis juliflora (Sw) DC pods (Leguminosae). Tropical Journal of Pharmaceutical Research, 17, 4, 661-668.
Martins, C. C. & Joung, J. H., Hwang, J., Kim, J. H., Sim, Deok, Y. Si., Im, E., Parque, J. E, Parque, W. Y., Shim, B. S., Kim, B. & Kim, S. H. (2021). Phyotochemical candidates repurposing for câncer therapy and their molecular. Seminars in Cancer Biology, 68, 1, 164-174.
Costa, M. M. (2020). Fatores de risco de melanoma em uma população latino-americana. Anais Brasileiros de Dermatologia, 95, 4, 529-531.
Nascimento, M. P. M. (2017). Potencial acaricida do extrato aquoso de algarobeira Prosopis juliflora no controle do ácaro Tetranychus bastosi na cultura do pinhão-manso. Dissertação de Mestrado, Serra Talhada, PE, Brasil.
Pretti, K., Sharma, R. A. & Acarwal, M. (2017). Isolation and identification of flavonoids from Prosopis juliflora. Mintage Journal of Pharmaceutical and Medical Sciences, 6, 1, 1-3.
Ricón, F., Muñoz, J. & Ramirez, P. (2014). Physicochemical and rheological characterization of Prosopis juliflora seed gum aqueous dispersions. Food Hydrocoll¸ 35, 1, 357-357.
Safe, S. & Kasiappan, R. (2016). Natural products as Mechanism-based anticancer agents: Sp. transcription factors as targets. Phytotherapy Research, 30, 11, 1723-1732.
Souza, B. C., Silva, D. H. M., Miyashiro, D., Kakizaki, P. & Valente, N. Y. S., (2020). Clinical-pathological analysis of acral melanoma in a single center: study of 45 cases. Revista da Associação Médica Brasileira,6,10, 1391-1395.
Urban, K., Mehrmal, S., Uppal P., Giesey, R. L. & Delost, G. R. (2021). The global burden of skin cancer: a longitudinal analysis of the Global Burden of Disease Study, 1990-2017. JAAD International, 2,3, 98-108.
Vedak S. & Raut S.V. (2014). Estudo de compostos antibacterianos do extrato metanólico da casca de Prosopis juliflora (Vilayati babhul). International Journal Pharmaceutical Science Business Management 2014; 2: 1-14.
Viana, A. R., Marzari, J., Wergutz, J. & Krause, L. M. F. (2017). Produtos bioativos na prevenção e no tratamento do Câncer, em especial o melanona. Disciplinarum Scientia, 18, 3, 511-528.
Yip, K. T., Zhong, Y. Z., Putz, N. S. S., Autzen, J., Gasper, R., Hofmann, E., Scherkenbeck, J. & Stoll, R. (2016). Small molecules antagonize the MIA-Fibronectin interaction in malignant melanoma. Scientific Reports, 6, 25119, 1-12.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Giani Maria Cavalcante; Patrícia Lira Barbosa ; Adrielle Antônia da Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
