Identification of alleles for sweet phenotype in local corn varieties in southern Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v10i4.14310

Keywords:

Zea mays; On farm conservation; Sugary1; Shrunken2; Germplasm characterization; Xenia phenomenon.

Abstract

Sweet corn (Zea mays L.) has high levels of sugar in the endosperm, being used for self-consumption by families in the far west of the state of Santa Catarina (FWSC), southern Brazil. The present work aimed to identify the genes responsible for the sweet phenotype and to characterize morphologically nine local corn varieties conserved in this region. The allelic tests proved the presence of two known recessive alleles (sugary1 mutant/sweet and shrunken2 mutant/super sweet) in eight of the nine varieties studied, and a third gene of unidentified genetic origin. The morphological characterization of ear and grain showed a similarity between the varieties for qualitative characters and a greater variation between and within the varieties for quantitative characters. The cluster analysis divided the materials into four groups and one isolated variety whose gene encoding the sweet phenotype can be a rare gene or still unknown. The results point to the possibility of a new allele having been selected under the specific conditions of the FWSC region, which presents environmental and social factors that influence the diversification of the Zea genus. The continuation of genetic studies on the sweet phenotype of this variety and the development of integrated strategies of in situ on farm and ex situ conservation and participatory breeding in FWSC can contribute to the expansion of the genetic variability of sweet corn and encourage conservation and use of this local germplasm.

Author Biographies

Rosenilda de Souza , Federal University of Santa Catarina

Agronomist, PhD. in Plant Genetic Resources at Federal University of Santa Catarina, Crop Science Department.

Part of the PhD. thesis of first author at Federal University of Santa Catarina.

 

Juliana Bernardi Ogliari, Federal University of Santa Catarina

Agronomist, PhD in Genetics and Plant Breeding, Professor at Federal University of Santa Catarina, Crop Science Department.

Corresponding author: juliana.bernardi@ufsc.br,  phone +55 (48) 3721-5328, fax: +55 (48) 3721-5335

Rose Mari Seledes, Federal University of Santa Catarina

Agronomist, MSc. in Plant Genetic Resources at Federal University of Santa Catarina, Crop Science Department. 

 

Wagner Bastos dos Santos Oliveira, Federal University of Santa Catarina

Biologist, PhD in Plant Genetic Resources at Federal University of Santa Catarina, Crop Science Department.

 

Tassiane Terezinha Pinto, Federal University of Santa Catarina

Biologist, PhD in Plant Genetic Resources at Federal University of Santa Catarina, Crop Science Department.

 

References

Almeida, C., Amorin, E. P., Barbosa Neto, J. F., Cardoso Filho, J. A. & Sereno, M. J. C. M. (2011). Genetic variability in populations of sweet corn, common corn and teosinte. Crop Breeding and Applied Biotechnology, 11 (1), 64-69. doi.org/10.1590/S1984-70332011000100009.

Almekinders, C. J. M. & Elings, A. (2001). Collaboration of farmers and breeders: participatory crop improvement in perspective. Euphytica, 122 (3), 425-438.

Boyer, C. D. & Shannon, J. C. (1984). The use of endosperm genes for sweet corn improvement. Plant Breeding Review, 1, 139-161.

Cardoso, E. T., Sereno, M. J. C, M. & Barbosa, Neto J. F. B. (2002). Heritability estimates for quality and ear traits in sweet corn. Crop Breeding and Applied Biotechnolgy, 2 (4). doi.org/10.12702/1984-7033.v02n04a01.

Chambers, J. M. (2008). Software for data analysis: programming with R. Springer, New York, NY.

Coe, J. R. E. H. (1985). Phenotypes in corn: control of pathways by alleles, time and place. In Freeling, M. (ed). Plant genetics. New Series, New York 35, 509–521.

Coradin, L. (2006). Parentes silvestres das espécies de plantas cultivadas. MMA-SBF, Brasília.

Costa, F. M., Silva, N. C. A. & Ogliari, J. B. (2016). Maize diversity in southern Brazil: indication of a microcenter of Zea mays L. Genetic Resources and Crop Evolution, 64 (4), 681-700. doi.org/10.1007/s10722-016-0391-2.

Denney, J. O. (1992). Xenia includes metaxenia. HortScience, 27 (7), 722-728.

Goldman, I. L. & Tracy, W. F. (1994). Kernel protein concentration in sugary-1 and shrunken-2 sweet corn. HortScience, 29 (3), 209-210.

Hammer. Ø., Harper, D. A. T. & Ryan, P. D. (2001). PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron, 4 (1), 1–9.

IBPGR, C. (1991). Descriptors for maize. International Board for Plant Genetic Resources, Rome and International Maize and Wheat Improvement Center, Mexico City, Rome.

Jha, S. K., Singh, N. K. & Agrawal, P. K. (2016). Complementation of sweet corn mutants: a method for grouping sweet corn genotypes. Journal of Genetics, 95 (1), 183-187. doi.org/10.1007/s12041-015-0608-8.

Kiesselbach, T. A. (1926). The immediate effect of gametic relationship and of parental type upon the kernel weight of corn. Research Bulletin: Bulletin of the Agricultural Experiment Station of Nebraska, 33, 1-69.

Machado, A. T. (2014). Construção histórica do melhoramento genético de plantas: do convencional ao participativo. Revista Brasileira de Agroecologia, 9 (1), 35-50.

MAPA (1997). Descritores mínimos de milho (Zea mays L.): sistema de proteção de cultivares. Diário Oficial da União, Brasília.

Mingoti, A. S. (2005). Análise de dados através de métodos de estatística multivariada: uma abordagem aplicada. UFMG, Belo Horizonte.

Ogliari, J. B. & Alves, A. C. (2007). Manejo e uso de variedades de milho como estratégia de conservação em Anchieta. In: Boef, W. S., Thijssen, M. H., Ogliari, J. B. & Sthapit, B. (Eds.), Biodiversidade e Agricultores: fortalecendo o manejo comunitário. Porto Alegre: L&PM, 226-234.

Ogliari, J. B. (2018). Presença do milho geneticamente modificado em um microcentro de diversidade, no Sul do Brasil. In: Delgado FDC (Ed.), Cuadernos de la Biored Iberoamérica CYTED, Cuaderno 6, Merida: Universidad Politécnica Territorial de Merida, 6, 31 -45.

Ogliari, J. B., Kist, V. & Canci, A. (2013). The participatory genetic enhancement of a local maize variety in Brazil. In: Boef, W. S., Subedi, A., Peroni, N., Thijssen, M. & O’Keeffe, E. (Eds.), Community biodiversity management, promoting resilience and the conservation of plant genetic resources.1 ed. Routledge, Oxon, 265-271.

Oliveira Jr., L. F. G, Pereira, M. G. & Bressan-Smith, R. (2006). Caracterização e avaliação agronômica de híbridos e linhagens de milho doce (su1). Horticultura Brasileira, 24 (3), 283-288. doi.org/10.1590/S0102-05362006000300003.

Reichert Jr., F. W., Souza, R., Pinto, T. T., Maghelly, O., Seledes, R. M., Avila, L. N. V. & Ogliari, J. B. (2020). Diversity of crops conserved by family farmers in the extreme west of Santa Catarina, southern Brazil. In: Mossi, A. J., Petry, C. & Reichert Jr., F. W. (Eds.), Agroecology insights, experiences and perspectives. 1 ed. Nova Publishers, Nova York, 141-161.

Reifschneider, F. J. B., Gama, E. E. G. & Reis, N. V. B. (1984). Milhos-doces: Superdoce (BR 400), Doce-de-ouro (BR 401) e Doce cristal (BR 402). Horticultura Brasileira 2 (2), 53-54.

Silva, N. C. A., Vidal, R. & Ogliari, J. B. (2017). New popcorn races in a diversity microcenter of Zea mays L. in the far west of Santa Catarina, southern Brazil. Genetic Resources and Crop Evolution, 64 (6), 1191-1204. doi.org/10.1007/s10722-016-0429-5.

Silva, N. C. A., Vidal, R., Costa, F. M., Vaio, M. & Ogliari, J. B. (2015). Presence of Zea luxurians (Durieu and Ascherson) Bird in southern Brazil: implications for the conservation of wild relatives of maize. PloS ONE, 10 (10), e0139034. doi.org/10.1371/journal.pone.0139034.

Silva, N. C. A., Vidal, R., Ogliari, J. B., Costich, D. E. & Chen, J. (2020). Relationships among American popcorn and their links with landraces conserved in a microcenter of diversity. Genetic Resources and Crop Evolution, 67 (7), 1733-1753. doi.org/10.1007/s10722-020-00935-2.

Souza, R., Ogliari, J. B. & Pinto. T. T. (2020). Analysis of on farm conservation of sweet corn in a diversity microcenter of Zea mays L. in southern. Maydica, 65 (9).

Strickberger, M. W. (1968). Genetics. Macmillan, New York.

Teixeira, F. F., Guimarães, C., Pinto, M. D. O., Pereira Filho, I. A., Coelho, R., Araújo, G. D. R. & Pontello, I. D. O. (2019). Catálogo de acessos do Banco Ativo de Germoplasma de Milho com grãos do tipo doce. Embrapa Milho e Sorgo-Documentos (INFOTECA-E), Sete Lagoas.

Teixeira, F. F., Paes, M. C. D., Gama, E. G., Pereira Filho, I. A., Miranda, R. A., Guimarães, P. E. O., Parentoni, S. N., Cotta, L. V., Meirelles, W. F., Silva, A. R. & Machado, J. R. A. (2014). BRS Vivi: single-cross super sweet corn hybrid. Crop Breeding and Applied Biotechnology, 14 (2), 124-127. doi.org/10.1590/1984-70332014v14n2c21.

Tracy, W. F. (2001). Sweet corn. In: Hallauer, A. R. (Ed), Specialty corn. CRC Press, B. Raton, 155-198.

Zeven, A. C. (1998). Landraces: a review of definitions and classifications. Euphytica, 104 (2), 127-139.

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Published

18/04/2021

How to Cite

SOUZA , R. de; OGLIARI, J. B.; SELEDES, R. M.; OLIVEIRA, W. B. dos S.; PINTO, T. T. Identification of alleles for sweet phenotype in local corn varieties in southern Brazil . Research, Society and Development, [S. l.], v. 10, n. 4, p. e46510414310, 2021. DOI: 10.33448/rsd-v10i4.14310. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/14310. Acesso em: 7 may. 2021.

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Section

Agrarian and Biological Sciences