Aspiration cytology of popliteal lymph nodes as a diagnostic method of canine visceral leishmaniasis (CVL) in areas of socioeconomic vulnerability
DOI:
https://doi.org/10.33448/rsd-v10i5.14457Keywords:
Amastigotes; Leishmania spp; Macrophages; Onychogryphosis.Abstract
Leishmaniasis is an endemic disease in Goiás and has high lethality owing to its high zoonotic potential. In dogs, the disease manifests based on the parasitic load, with clinical presentation as the starting for diagnosis. Diagnostic methods are used to confirm the disease, with emphasis on lymph node aspiration cytology, which is a high-standard technique owing to its low cost, ease of performance, and high specificity. The aim of this study was to demonstrate aspiration lymph node cytology as a diagnostic method for canine visceral leishmaniasis (CVL) in areas containing low-income populations with poor access to molecular tests. Three dogs with suspected CVL were studied at Veterinary Hospital of the Federal University of Jataí, in which popliteal lymph node cytology was performed, owing to easy access, and screening and confirmatory examinations were undertaken by the Central Public Health Laboratory. The results showed three positive animals in the cytology and only one positive in the other tests; therefore, there was the possibility of the occurrence of false negatives in serological tests given the time required for seroconversion. Thus, cytology is an important diagnostic technique in areas where molecular tests are not available.
References
Abbiati, T. C., Freitas, D. M., Alves, L. C., Freitas, B. G., Rezende, R. S., Barbosa, S. G., Teixeira, A. L., Jorge, A., Santos, S. M. & Lopes M. C. (2019) Leishmaniose visceral canina: Relato de caso. PUBVET Medicina Veterinária,13(34), 1-8.
Andrade-Filho, J. D., Scholte, R. G. C., Amaral, A. L. G., Shimabukuro, P. H. F., Carvalho, O. S. & Caldeira, R. (2017). Occurrence and probability maps of lutzomyia longipalpis and lutzomyia cruzi (díptera: psychodidae: phlebotominae) in brazil. Journal of Medical Entomology, 54(5): 1430-1434.
Azevedo, E. M. R., Duarte, S. C., Costa, H. X., Alves, C. E. F., Neto, O. J. S., Jayme, V. S. & Linhares, G. F. C. (2011) Estudo da leishmaniose visceral canina no município de Goiânia, Goiás, Brasil. Revista de Patologia Tropical,40(2).
Barros, A. L. C., Benites, A. P., Mateus, N. L. F. & Fernandes, C.E. (2019). Histopathological alterations in the reproductive tract of male dogs with canine visceral leishmaniasis. Semina: ciências agrárias, 40(2), 665-676.
Benassi, J. C., Benvenga, G. U., Ferreira, H. L., Soares, R. M., Silva, D. T., Pereira, V. F., Ruiz, V. L. A. & Oliveira, T. M. F. S. (2018). Molecular and serological detection of leishmania spp. in horses from an endemic área for canine visceral leishmaniasis in southeastern brazil. Pesquisa Brasileira Veterinária, 38(6), 1058-1063.
Conti, R., Lane, V. M., Montebello, L. & Junior, V. P. (2016). Visceral leishmaniasis epidemiologic evolution in timeframes, based on demographic chages and scientific achievements in Brazil. Jornal of Vector Borne Diseases, 53(2), 99-104.
Dias, R. C. F., Pasquali, A. K. S., Thomaz-Soccol, V., Pozzolo, E. M., Chiyo, L., Alban, S. M., Fendrich, R. C., Almeida, R. A. A., Ferreira, F. P., Caldart, E. T., Freire, R. L., Mitsuka-Braganó, R. & Júnior, A. B. (2019). Autochthonous canine visceral leishmaniasis cases occur in paraná state since 2012: isolation and identification of Leishmania infantum. Brazilian Journal of Veterinary Parasitology, 29(1).
Escobar, T. A., Dowich, G., Cantele, L.C., Zuravski, L., Ferrareze, P. A. G., Duarte, C. A., Silva, L. K. R., Lübeck, I. & Manfredini, V. (2019). Molecular detection of leishmania spp. in Brazilian cross-border south region mammalian hosts. Transboundary and Emerging Diseases, 67(2), 476-480.
Goiás, 2017. Marques, S. F. F, Jesus, A. K. & Sousa, F. A. Leishmaniose Visceral em Goiás – Aspectos epidemiológicos. Boletim Epidemiológico [online]. 2017 http://www.sgc.goias.gov.br/upload/arquivos/2017-10/leishmaniose-visceral-em-goias---aspectos-epidemiologicos.pdf.
Goiás, 2019. Reis L. A. L., Marques S. F. F. Leishmaniose visceral em Goiás o ano de 2018. Boletim Epidemiológico. https://www.saude.go.gov.br/images/imagens_migradas/upload/arquivos/2019-10/boletim-epidemiologico---leishmaniose-visceral-em-goias-no-ano-de-2018-1.pdf.
Limeira, C. H., Alves, C. J., Azevedo, S. S., Santos, C. S. A. B., Melo, M. A., Soares, R. R., Barnabé, N. N. C. & Rodrigues, G. Q. (2019). Clinical aspects and diagnosis of leishmaniasis in equids: a systematic review and meta-analysis. Brazilian Journal of Veterinary Parasitology, 28(4), 574-581.
Liu, D. & Uzonna, J. E. (2012). The early interaction of Leishmania with macrophages and dendritic cells and its influence on the host immune response. Frontiers in Cellular and Infection Microbiology, 2(83), 1-8.
Lopes, E. G., Sevá, A. P., Ferreira, F., Nunes, C. M., Keid, L. B., Hiramoto, R. M., Ferreira, H. L., Oliveira, M. F. S., Bigotto, M. F. D., Galvis-Ovallos, F., Galati, E. A. B. & Soares, R. M. (2017). Serological and molecular dianostic tests for canine visceral leishmaniasis in Brazilian endemic área: one out of live seronegative dogs are infected. Epidemiology and Infection, 145 (12), 2436-2644.
Maia, C. & Campino, L. (2008). Methods for disgnosis of canine leishmaniasis and immune response to infection. Veterinary Parasitology, 158(4), 274-287.
Noli, C. & Saridomichelakis, M. N. (2014). An update on the diagnosis and treatment of canine leishmaniosis caused by Leishmania infantum (syn. l. chagasi). Veterinary Journal ,202(3), 425-435.
Oliveira, T. S., Miranda, F. G., Ribeiro, V. M. & Santos, R. L. (2011). Análise de métodos diagnósticos para leishmaniose visceral canina a partir de um levantamento de casos atendidos em uma clínica veterinária na cidade de Belo Horizonte, MG. Medvep – Revista Cientifica de Medicina Veterinária – Pequenos Animais e Animais de Estimação, 9(31): 692- 696.
OMS - ORGANIZAÇÃO MUNDIAL DA SAÚDE. Global leishmaniasis update, 2006–2015: a turning point in leishmaniasis surveillance. 2017 http://apps.who.int/iris/bitstream/10665/258973/1/ WER9238.pdf? Ua = 1.
Pal, S. & Biswas, B. (2018). Fine-needle aspiration cytology of leishmanial lymphadenitis in an HIV-reactive patient: Report of a rare case. Tropical Parasitology ,8(1), 50-52.
Paltrinieri, S., Gradoni, L., Roura, X., Zatelli, A. & Zini, E. (2016). Laboratory tests for diagnosing and monitoring canine leishmaniasis. Veterinary Clinical Pathology, 45(4), 552-578.
Paltrinieri, S., Solano-Gallego, L., Fondati, A., Lubas, G., Gradoni, L., Castagnaro, M., Crotti, A., Maroli, M., Oliva, G., Roura, X., Zatelli, A. & Zini, E. (2010). Guidelines for diagnosis and clinical classification of leishmaniasis in dogs. Journal of the American Veterinary Medical Association, 236(11), 1184-1191.
Ramos, R. A. N., Ramos, C. A. N., Santos, E. M. S., Araújo, F. R., Carvalho, G. A., Faustino, M. A. G. & Alves, L. C. (2013). Quantification of Leishmania infantum DNA in the bone marrow, lymph node and spleen of dogs. Brazilian Journal of Veterinay Parasitology, 22(3), 346-350.
Ribeiro, R. R., Michalick, M. S. M., Silva, M. E., Santos, C. C. P., Frézard, F. J. G. & Silva, S. M. (2018). Canine leishmaniasis: an overview of the current status and strategies for control. Biomed Research International, 2018(6), 1-12.
Santos, R. L. & Oliveira, A. R. (2019). Leishmaniasis in non-human primates: Clinical and pathological manifestations and potential as reservoirs. Journal of Medical Primatology, 49(1), 34-39.
Saridomichelakis, M. N., Mylonakis, M. E., Leontides, L. S., Koutinas, A. F., Billinis, C. & Kontos, V. I. (2005). Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic dogs. The American Journal of Tropical Medicina and Hygiene, 73(1), 82-86.
Silva, D. A., Madeira, M. F., Abrantes, T. R., Filho, J. L. B. & Figueiredo, F. B. (2013). Assessment of serological tests for the diagnosis of canine visceral leishmaniasis. Veterinary Journal, 195(2), 252-253.
Silva, D. T., Alves, M. L., Spada, J. C. P., Silveira, R. C. V, Oliveira, T. M. F. S. & Starke-Buzzeti, W. A. (2018). Neutrophils, eosinophils, and mast cells in the intestinal wall of dogs naturally infected with leishmania infantum. Brazilian Journal of Veterinary Parasitology, 27(4), 430-438.
Silva, J. A. O., Silva, F. J., Macedo, L. O., Santos, C. V. B., Alves, L.C., Ramos, R. A. N., Faustino, M. A. G. & Carvalho, G. A. C. (2019). Sandflies in an endemic área for visceral leishmaniasis in northeastern brazil. Brazilian Journal of Veterinary Parasitology, 28(4), 569-573.
Teles, N. M. M., Agostini, M. A. P., Bigeli, J. G., Noleto, R.V., Oliveira, J. D. & Oliveira-Junior, M. P. (2012). Molecular and parasitological detection of Leishmania spp. in dogs caught in Palmas, TO, Brazil. Revista Brasileira de Parasitologia Veterinária ,21(3), 278-282.
Tilley, L. P. & Smith Jr, F. W. K. (2008). Consulta veterinária em cinco minutos. Espécies canina e felina. Manole.
Torrecilha, R. B. P., Utsunomiya, Y. T., Bosco, A.M., Almeida, B. F., Pereira, P. P., Narciso, L. G., Pereira, D. C. M., Baptistiolli, L., Calvo-Baldo, L. & Courtnenay, O. (2016). Correlations between peripheral parasite load and common clinical and laboratory alterations in dogs with visceral leishmaniasis. Preventive Veterinary Medicine, 132, 83-87.
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Copyright (c) 2021 Antônio Carlos Severino Neto; Gabriel Lopes Germano; Thâmara Rossi Martins da Silva; Luiz Antônio de Jesus Sousa; Priscila Gomes de Oliveira; Reiner Silveira de Moraes; Dirceu Guilherme de Sousa Ramos
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