The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation
DOI:
https://doi.org/10.33448/rsd-v10i5.14765Keywords:
Enterococcus faecalis; Apical foramen; Disinfection; Root canal therapy.Abstract
To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation.
References
Alves, F. R., Almeida, B. M., Neves, M. A., Moreno, J. O., Rocas, I. N., & Siqueira, J. F. (2011) Disinfecting oval-shaped root canals: effectiveness of different supplementary approaches. J Endod.37, 496-501.
Brandao, P. M., de Figueiredo, J. A. P., Morgental, R. D., Scarparo, R. K., Hartmann, R. C., Waltrick, S. B. G., et al (2019). Influence of foraminal enlargement on the healing of periapical lesions in rat molars. Clin Oral Investig, 23, 1985-1991.
Borlina, S. C., de Souza, V., Holland, R., Murata, S. S., Gomes-Filho, J. E., Dezan Junior, E., et al (2010). Influence of apical foramen widening and sealer on the healing of chronic periapical lesions induced in dogs' teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod.109, 932-940.
Brito, P. R., Souza, L. C., Machado de Oliveira, J. C., Alves, F. R., De-Deus, G., Lopes, H. P., et al. (2009). Comparison of the effectiveness of three irrigation techniques in reducing intracanal Enterococcus faecalis populations: an in vitro study. J Endod, 35, 1422-1477.
Card, S. J., Sigurdsson, A., Orstavik, D., & Trope, M. (2002). The effectiveness of increased apical enlargement in reducing intracanal bacteria. J Endod, 28, 779-783.
Carvalho, M. C., Zuolo, M. L., Arruda-Vasconcelos, R., Marinho, A. C. S., Louzada, L. M., Francisco, P. A., et al (2019). Effectiveness of XP-Endo Finisher in the reduction of bacterial load in oval-shaped root canals. Braz Oral Res.33:e021.
Coldero, L. G., McHugh, S., MacKenzie, D., & Saunders, W. P. (2002). Reduction in intracanal bacteria during root canal preparation with and without apical enlargement. Int Endod J,35, 437-446.
Cruz Junior, J. A., Coelho, M. S., Kato, A. S., Vivacqua-Gomes, N., Fontana, C. E., Rocha, D. G., et al (2016). The Effect of Foraminal Enlargement of Necrotic Teeth with the Reciproc System on Postoperative Pain: A Prospective and Randomized Clinical Trial. J Endod, 42, 8-11.
De Deus, Q. D. (1975). Frequency, location, and direction of the lateral, secondary, and accessory canals. J Endod, 1, 361-366.
de Souza Filho, F. J., Benatti, O., & de Almeida, O. P. (1987). Influence of the enlargement of the apical foramen in periapical repair of contaminated teeth of dog. Oral Surg Oral Med Oral Pathol, 64, 480-484.
Frota, M. M. A., Bernardes, R. A., Vivan, R. R., Vivacqua-Gomes, N., Duarte, M. A. H., & Vasconcelos, B. C. (2018). Debris extrusion and foraminal deformation produced by reciprocating instruments made of thermally treated NiTi wires. J Appl Oral Sci, 26:e20170215.
Guivarc'h, M., Ordioni, U., Ahmed, H. M., Cohen, S., Catherine, J. H., & Bukiet, F. (2017). Sodium Hypochlorite Accident: A Systematic Review. J Endod, 43, 16-24.
Lin, L. M., & Rosenberg, P. A. (2011). Repair and regeneration in endodontics. Int Endod J, 44, 889-906.
Machado, M. E., Nabeshima, C. K., Leonardo, M. F., Reis, F. A., Britto, M. L., & Cai, S. (2013). Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J, 46, 1083-1087.
Mickel, A. K., Chogle, S., Liddle, J., Huffaker, K., & Jones, J. J. (2007). The role of apical size determination and enlargement in the reduction of intracanal bacteria. J Endod, 33,21-23.
Nabeshima, C. K., Caballero-Flores, H., Cai, S., Aranguren, J., Borges Britto, M. L., & Machado, M. E. (2014). Bacterial removal promoted by 2 single-file systems: Wave One and One Shape. J Endod, 40, 1995-1998.
Nair, P. N., Henry, S., Cano, V., & Vera, J. (2005). Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 99, 231-252.
Ricucci D, & Siqueira JF, Jr. (2008). Anatomic and microbiologic challenges to achieving success with endodontic treatment: a case report. J Endod,34, 1249-1254.
Ricucci, D., Siqueira, J. F., Jr., Bate, A. L., & Pitt Ford, T. R. (2009). Histologic investigation of root canal-treated teeth with apical periodontitis: a retrospective study from twenty-four patients. J Endod, 35, 493-502.
Ricucci, D., & Siqueira, J. F. (2010). Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod, 36, 1277-1288.
Schneider, S. W. (1971). A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol, 32, 271-275.
Sedgley, C. M., Lennan, S. L., & Appelbe, O. K. (2005). Survival of Enterococcus faecalis in root canals ex vivo. Int Endod J, 38, 735-742.
Shuping, G. B., Orstavik, D., Sigurdsson, A., & Trope, M. (2000). Reduction of intracanal bacteria using nickel-titanium rotary instrumentation and various medications. J Endod, 26, 751-755.
Silva, E. J., Menaged, K., Ajuz, N., Monteiro, M. R., & Coutinho-Filho, T. de S. (2013). Postoperative pain after foraminal enlargement in anterior teeth with necrosis and apical periodontitis: a prospective and randomized clinical trial. J Endod, 39, 173-176.
Silva Santos, A. M., Portela, F., Coelho, M. S., Fontana, C. E., & De Martin, A. S. (2018). Foraminal Deformation after Foraminal Enlargement with Rotary and Reciprocating Kinematics: A Scanning Electronic Microscopy Study. J Endod, 44, 145-148.
Siqueira, J. F., Jr., Rocas, I. N., Paiva, S. S., Guimaraes-Pinto, T., Magalhaes, K. M., & Lima, K. C. (2007). Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 104, 122-130.
Valverde, M. E., Baca, P., Ceballos, L., Fuentes, M. V., Ruiz-Linares, M., & Ferrer-Luque, C. M. (2017). Antibacterial efficacy of several intracanal medicaments for endodontic therapy. Dent Mater J, 36, 319-324.
Versiani, M. A., Leoni, G. B., Steier, L., De-Deus, G., Tassani, S., Pecora, J. D., et al (2013). Micro-computed tomography study of oval-shaped canals prepared with the self-adjusting file, Reciproc, WaveOne, and ProTaper universal systems. J Endod, 39, 1060-1066.
Yadav, S. S., Shah, N., Naseem, A., Roy, T. S., & Sood, S. (2014). Effect of "apical clearing" and "apical foramen widening" on apical ramifications and bacterial load in root canals. Bull Tokyo Dent Coll, 55, 67-75.
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