Immunohistochemical expression of macrophages in chronic periapical lesions
DOI:
https://doi.org/10.33448/rsd-v10i7.16622Keywords:
Periapical granuloma; Radicular cyst; Immunohistochemistry; CD68 macrophage.Abstract
Objective: To analyze the expression of macrophages in periapical granulomas (PGs) and radicular cysts (RCs). Methodology: We selected 264 cases of chronic periapical lesions stored at the Laboratory of Pathology, Dental School of Pernambuco (FOP)/UPE, including 89 PGs and 175 RCs. Seventy-nine cases, 23 PGs and 56 RCs, were submitted to immunohistochemical analysis by the streptavidin-biotin method using anti-CD68 antibody. The chi-square and Fisher’s exact tests were used to determine the existence of associations with categorical and clinical variables, adopting a 95% confidence level (p≤0.05). Results: We observed immunoexpression of CD68 in 83% of cases. The number of CD68+ cells (score 3 or 4) was higher in PGs, with predominantly intense staining. Significant differences in the number of CD68+ cells and staining intensity were observed between the chronic periapical lesions analyzed. Conclusion: The presence of CD68+ cells in periapical tissues may indicate the development, maintenance and/or severity of the inflammation-mediated immune response, which is more intense in PG.
References
Álvares, P. R., Arruda, J. A. A., Silva, L. P., Nascimento, G. J. F., Silveira, M. M. F., & Sobral, A. P. V. (2017). Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions. Brazilian Oral Research, 3, 31:e 51.
Alvares, P. R., Arruda, J. A. A., Silva, V. O., Silva, L. P., Nascimento, G. J. F., Silveira, M. M. F., & Sobral, A. P. V. (2018). Immunohistochemical Analysis of Cyclooxygenase-2 and Tumor Necrosis Factor Alpha in Periapical Lesions. Journal of Endodontics, 44(12), 1783-87.
Azeredo, S. V., Brasil, S. C., Antunes, H., Marques, F. V., Pires, F. R., & Armada, L. (2017). Distribution of macrophages and plasma cells in apical periodontitis and their relationship with clinical and image data. Journal of Clinical and Experimental Dentistry, 9(9), 1060-e1065.
Bănică, A. C., Popescu, S. M., Mercuţ, V., Busuioc, J. C., Gheorghe, A. G., Traşcă, D. M., Brăila, A. D., & Moraru, A. I. (2018). Histological and immunohistochemical study on the apical granuloma. Romanian Journal of Morphology and Embryology, 59(3), 811–817.
Bracks, I. V., Armada, L., Goncalves, L. S., & Pires, F. R. (2014). Distribution of mast cells and macrophages and expression of interleukin-6 in periapical cysts. Journal of Endodontics, 40(1), 63–8.
Cassanta, L. T. C., Rodrigues, V., Violatti-Filho, J. R., Teixeira Neto, B. A., Tavares, V. M., Bernal, E. C. B. A., Souza, D. M., Araujo. M. S., Lima, P. S. A. de, & Rodrigues, D. B. R. (2017). Modulation of Matrix Metalloproteinase 14, Tissue Inhibitor of Metalloproteinase 3, Tissue Inhibitor of Metalloproteinase 4, and Inducible Nitric Oxide Synthase in the Development of Periapical Lesions. Journal of Endodontics, 43(7), 1122-1129.
Cavalla, F., Letra, A., Silva, R. M. & Garlet, G. P. (2021). Determinants of Periodontal/Periapical Lesion Stability and Progression. Journal of Dental Research, 100(1):29-36.
Galler, K. M., Weber, M., Korkmaz, Y., Widbiller, M. & Feuerer, M. (2021). Inflammatory Response Mechanisms of the Dentine-Pulp Complex and the Periapical Tissues. International Journal of Molecular Sciences, 2; 22(3): 1480
Graunaite, I., Lodiene, G., Maciulskiene, V. (2012). Pathogenesis of Apical Periodontitis: A Literature Review. Journal of oral e maxillofacial research, 2(4), 1011-23.
Koivisto, T., Bowles, W. R., & Roherer, M. (2012). Frequency and distribution of radiolucent jaw lesions: a retrospective analysis of 9,723 cases. Journal of Endodontics, 38(6), 729-32.
Leonardi, R., Caltabiano, R., & Loreto, C. (2005). Collagenase-3 (MMP-13) is expressed in periapical lesions: an immunohistochemical study. International Endodontic Journal, 38(5), 297–301.
Liapatas, S., Nakou, M., & Rontogianni, D. (2003). Inflamatory infiltrate of chronic periradicular lesions: an immunohistochemical study. International Endodontic Jounal, 36(7), 464-71.
Li, J., Hsu, H. C., & Mountz, J. D. (2013). The dynamic duo-inflammatory M1 macrophages and Th17 cells in rheumatic diseases. Journal of Rheumatology and Orthopedics, 1(1), 4.
Lin, S. K., Hong, C. Y., Chang, H. H., Chiang, C. P., Chen, C. S., Jeng, J.H., & Kuo, M. Y. (2000). Immunolocalization of macrophages and transforming growth factor-beta 1 in induced rat periapical lesions. Journal of Endodontics, 26(6), 335-40.
Locati, M., Mantovani, A., & Sica A. (2013). Macrophage activation and polarization as an adaptive component of innate immunity. Advances in Immunology, 120, 163–84.
Maia, L.M., Espaladori, M.C., Diniz, J.M.B., Tavares, W.L.F., de Brito, L.C.N., Vieira, L.Q. & Sobrinho, A.P.R. (2020). Clinical endodontic procedures modulate periapical cytokine and chemokine gene expressions. Clinical Oral Investigation, 24(10):3691-3697.
Mantovani, A., Biswas, S. K., Galdiero, M. R., Sica, A., & Locati, M. (2013). Macrophage plasticity and polarization in tissue repair and remodelling. The Journal of Pathology, 229(2), 176–85.
Mantovani, A., Sica, A., & Locati, M. (2007). New vistas on macrophage differentiation and activation. European Journal of Immunology, 37(1), 14–6.
Marton, I. J., & Kiss, C. (2000). Protective and destructive immune reactions in apical periodontitis. Oral Microbiology and Immunology, 15(3), 139–50.
Metzger, Z. (2000). Macrophages in periapical lesions. Endodontic & Dental Traumatology, 16(1), 1-8.
Rodini, C. O.; Batista, A. C.; & Lara, V. S. (2004). Comparative immunohistochemical study of the presence of mast cells in apical granulomas and periapical cysts:Possible role of mast cells in the course of human periapical lesions. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 97(1), 59-63.
Rodini, C. O., & Lara, V. S. (2001). Study of the expression of CD68+ macrophages and CD8+ T cells in human granulomas and periapical cysts. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 92(2), 221–7.
Suzuki, T., Kumamoto, H., Ooya, K., & Motegi, K. (2001). Immunohistochemical analysis of CD1a-labeled Langerhans cells in human dental periapical inflammatory lesions--correlation with inflammatory cells and epithelial cells. Oral Diseases, 7(6), 336-43.
Weber, M., Schlittenbauer, T., Moebius, P., Büttner-Herold, M., Ries, J., Preidl, R., Geppert, C. I., Neukam, F.W., & Wehrhan, F. (2018). Macrophage polarization differs between apical granulomas, radicular cysts, and dentigerous cysts. Clinical Oral Investigations, 22(1), 385-94.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Zilda Betânia Barbosa Medeiros de Farias; Jade Souza Cavalcante; Anne Caroline de Lima; Emanuel Savio de Souza Andrade; Márcia Maria Fonseca da Silveira; Ana Paula Veras Sobral
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
