Effects of tobacco smoke inhalation on mast cell density and collagenation process in rat gingival tissue

Authors

DOI:

https://doi.org/10.33448/rsd-v10i8.17208

Keywords:

Collagen; Gingiva; Mast cells; Rat; Smoking tobacco.

Abstract

Tobacco is one of the main risk factors for periodontal diseases, although this relationship is not fully understood. The aim of the study was to perform a histopathological evaluation of the gingiva of rats exposed to tobacco smoke inhalation. Thirty Wistar rats were grouped into three groups: rats not exposed to tobacco smoke (n=10); rats exposed to tobacco smoke inhalation for 30 days (n=10); rats exposed to tobacco smoke inhalation for 60 days (n=10). After euthanasia, plasma and gingiva were collected. In the 60-day group, a higher frequency of apoptosis, exocytosis, necrosis, hyperkeratosis, higher density of mast cells and lower percentage of collagen were observed (p>0,05). A weak, inversely proportional correlation was observed between the percentage of collagen and the density of mast cells (p>0,05). The inhalation of tobacco smoke negatively affected body mass gain besides provoking reduction in collagen percentage and increased mast cell density in the gingival tissue of rats. Thus, the findings play an important role in alerting health authorities about the deleterious effects of exposure to tobacco smoke, especially on gingival tissue.

References

Alexandridi, F., Tsantila, S., & Pepelassi, E. (2018). Smoking cessation and response to periodontal treatment. Aust Dent J, 63 (2), 140-149.

Almeida, A. F & Mussi, F. C. (2006). Smoking: knowledge, attitudes, habits and degree of dependence of young adults in Salvador. Rev Esc Enferm USP, 40 (4), 456-463.

Amaral, E. P., Rosa, R. C., Etchebehere, R. M., Nogueira, R. D., Volpon, J. B., Rodrigues, D. B. R & De Lima Pereira, S. A. (2020). Overexpression of HIF-1α and Morphological Alterations in the Tongue of Rats Exposed To Secondhand Smoke. Braz Dent J, 31 (3), 281-289.

Andreollo, N. A., Santos, E. F., Araújo, M. R., & Lopes, L. R. (2012). Rat's age versus human's age: what is the relationship?. ABCD. Arquivos Brasileiros de Cirurgia Digestiva, 25 (1), 49-51.

Balatsouka, D., Gotfredsen, K., Sorensen, L., Lindh, C. H & Berglundh, T. (2006). Effect of systemic administration of nicotine on healing in osseous defects. An experimental study in rabbits. Part II. Clin Oral Implants Res, 17 (5), 488-494.

Barnoya, J., & Glantz, S. A. (2004). Secondhand smoke: the evidence of danger keeps growing. Am J Med, 116 (3), 201–202.

Batista, A. C., Rodini, C. O., & Lara V. S. (2005). Quantification of mast cells in different stages of human periodontal disease. Oral Dis, 11 (4), 249-254.

Bertoldo, B. B., Etchebehere, R. M., Furtado, T. C. S., Faria, J. B., Silva, C. B., Araújo, M. F., Rodrigues, D. B. R & De Lima Pereira, S. A. (2019). Lingual salivary gland hypertrophy and decreased acinar density in chagasic patients without megaesophagus. Rev Inst Med Trop, 61, 1-6.

Bergström, J. (2004). Tobacco smoking and chronic destructive periodontal disease. Odontology, 92 (1), 1-8.

Bosco, A. F., Okamoto, t., Nagata, M. J. H & Machado, G. J. R. (2004). Efeitos da nicotina no processo de reparo de feridas cutâneas associadas à aplicação tópica de clorexedine. Rev Odonto Cienc, 19 (45), 207-213.

César Neto, J. B., Rosa, E. F., Pannuti, C. M & Romito, G. A. (2012). Smoking and periodontal tissues: a review. Braz Oral Res, 26(1), 25-31.

Chiu, H. T., Wu, H. D. I., & Kuo H. W. (2008). The relationship between self-reported tobacco exposure and cotinines in urine and blood for pregnant women. Sci Total Environ, 406, 331-336.

Colombo, G., Clerici M., Giustarini, D., Portinaro, N.M., Aldini, G., Rossi, R., Milzani, A., & Dalle-Donne, I. (2014). Pathophysiology of tobacco smoke exposure: recent insights from comparative and redox proteomics. Mass Spectrom Rev, 33(3), 183-218.

Dhall, S., Alama, T. R., Castro, A., Sarker, A. H., Mao, J. H., Chan, A., Hang, B., & Martins-Green, M. (2016). Tobacco toxins deposited on surfaces (third hand smoke) impair wound healing. Clin Sci (Lond), 130 (14), 1269-1284.

Duygulu, F., Karaoglu, S., Zeybek, N. D., Kaymaz, F. F., & Günes, T. (2006). The effect of subcutaneously injected nicotine on achilles tendon healing in rabbits. Knee Surg Sports Traumatol Arthrosc, 14 (8), 756-761.

Erdemir, E. O., Sönmez, I. S., Oba, A. A., Bergstrom, J., & Caglayan, O. (2010). Periodontal health in children exposed to passive smoking. J Clin Periodontol, 37 (2), 160-164.

Filippis, D., D‘Amico, A., & Iuvone, T. (2008). Cannabinomimetic control of mast cell mediator release: New perspective in chronic inflammation. J Neuroendocrinol, 20 (1), 20-25.

Gentner, N. J., & Weber, L. P. (2012). Secondhand tobacco smoke, arterial stiffness, and altered circadian blood pressure patterns are associated with lung inflammation and oxidative stress in rats. Am J Physiol Heart Circ Physiol, 302 (3), 818-825.

Golijanin, R., Kujundžić, B., Milosavljević, Z., Milovanović, D. R., Andjelković, Z., Obrenović, M., & Nikolić R. (2015). Morphometric analysis of collagen and inflammatory cells in periodontal disease. Vojnosanit Pregl, 72 (3), 219-224.

Hafez, S. M. N. A. (2019). Age related changes in the dermal mast cells and the associated changes in the dermal collagen and cells: A histological and electron microscopy study. Acta Histochem, 121 (5), 619-627.

Hay, E. D. (1999). Biogenesis and organization of extracellular matrix. FASEB J, 13 (2), 281-283.

Ijomone, O. M., Olaibi, O. K., & Nwoha, P. U. (2014). Effects of chronic nicotine administration on body weight, food intake and nitric oxide concentration in female and male rats. Pathophysiology, 21 (3), 185-190.

Johnson, G. K., & Guthmiller, J. M. (2007). The impact of cigarette smoking on periodontal disease and treatment. Periodontol, 44, 178-194.

Leite, F. R. M., Nascimento, G. G., Scheutz, F., & López R. (2018). Effect of Smoking on Periodontitis: A Systematic Review and Meta-regression. Am J Prev Med, 54 (6), 831-841.

Martinez, A. E. T., Silvério, K. G., & Rossa Júnior C. (2002). Efeito da nicotina na viabilidade e morfologia de fibroblastos - estudo in vitro. Pesq Odontol Bras, 16 (3), 234-238.

Influence of aging on the skin quality of white-skinned women: the role of collagen, elastic material density, and vascularization. Rev Bras Cir Plást, 28 (1), 41-48.

Oshi S. N., Oshi, D. C., Weaver, S., Agu, C. F., Smith, P. W., Ricketts Roomes, T., Meka, I. A., Rae, T., & Abel, W. D. (2018). A School-Based Study of the Influence of Students’ Relationship with Teachers on Their Cigarette Smoking Behaviour in Jamaican. Asian Pac J Cancer Prev, 19 (1), 7-12.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Ribeiro, F. A. Q., Carvalho, M. F. P., Pereira, C. S. B., & Tateno, D. A. (2015). Análise da concentração de colágeno tipo I e III presente no reparo de feridas tratadas com Mitomicina C em ratos. Arq Med Hosp Fac Cienc Med Santa Casa São Paulo, 60, 22-26.

Rosa, R. C., Pereira, S. C., Cardoso, F. A. G., Caetano, A. G., Santiago, H. A. R., & Volpon, J. B. (2017). Second hand tobacco smoke adversely affects the bone of immature rats. Clinics (São Paulo), 72 (12), 785-789.

Sampaio, N. D. de S., Santos, M. F. A. dos & Paz, F. A. do N. (2020). Complications caused by nicotine during the pregnancy period. Research, Society and Development, v.9 (7), e64897450. DOI: 10.33448/rsd-v9i7.4506.

Salum, A. W., Neto, J. B. C., & Sallum, E. J. (2007). Tabagismo e a doença periodontal. Revista Periodontia, 17 (2), 45-53.

Santiago, H. A., Zamarioli, A., Sousa Neto, M. D., & Volpon, J. B. (2017). Exposure to Secondhand Smoke Impairs Fracture Healing in Rats. Clin Orthop Relat Res, 475 (3), 894-902.

Skinovsky, J., Malafaia, O., Chibata, M., Tsumanuma, F., Panegalli Filho, F., & Martins, M.V . D. C. (2016). The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts. Rev Col Bras Cir, 43 (2), 087-092.

Souza, A. P & Line, S. R. P. (2002). The biology of matrix metalloproteinases. Rev Fac Odontol Bauru, 10 (1), 1-6.

Souza, R. S., & Pinhal, M. A. S. (2011). Interações em processos fisiológicos: a importância da dinâmica entre matriz extracelular e proteoglicanos. Arquivos Brasileiros de Ciências da Saúde, 36 (1), 48-54.

Sutton, J. D., Salas Martinez, M. L., & Gerkovich, M. M. (2017). Environmental Tobacco Smoke and Periodontitis in United States Non-Smokers, 2009 to 2012. J Periodontol, 88 (6), 565-574.

Vardavas, C. I & Nikitara, K. (2020). COVID-19 and smoking: A systematic review of the evidence. Tob Induc Dis, Essen. 18: 20.

Wang, X. D., Liu, C., Bronson, R. T., Smith, D. E., Krinsky, N. I., & Russel, M. (1999). Retinoid signaling and activator protein-1 expression in ferrets given beta-carotene supplements and exposed to tobacco smoke. J Natl Cancer Inst, 91 (1), 60-66.

Who - World Health Organization. (2015). Tobacco: relatório n° 339 de julho de 2015. http://www.who.int/mediacentre/factsheets/fs339/en/

Zia, S., Ndoye, A., Lee, T. X., Webber, R. J., & Grando, S. A. (2000). Receptor-mediated inhibition of keratinocyte migration by nicotine involves modulations of calcium influx and intracellular concentration. J Pharmacol Exp Ther, 293 (3), 973-981.

Downloads

Published

12/07/2021

How to Cite

FURTADO, T. C. de S. .; CHAVES, L. S. .; ESPINDULA, A. P. .; CARDOSO , F. A. G. .; TSUKIDE, J. C. .; LÉO, J. A. .; PEREIRA, S. A. de L. .; ROSA, R. C. . Effects of tobacco smoke inhalation on mast cell density and collagenation process in rat gingival tissue. Research, Society and Development, [S. l.], v. 10, n. 8, p. e29810817208, 2021. DOI: 10.33448/rsd-v10i8.17208. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17208. Acesso em: 22 nov. 2024.

Issue

Vol. 10 No. 8

Section

Health Sciences

License

Copyright (c) 2021 Taíssa Cássia de Souza Furtado; Liliane Salgado Chaves; Ana Paula Espindula; Fabrizio Antonio Gomide Cardoso ; Júlio César Tsukide; Jorge Alfredo Léo; Sanívia Aparecida de Lima Pereira; Rodrigo César Rosa

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.