Aspergillus spp. isolates from bovine rumen can increase the digestibility and degradability of forages
DOI:
https://doi.org/10.33448/rsd-v10i8.17340Keywords:
Cattle; Ruminant nutrition; Fungi of rumen; Tropical pasture; Fungi colonization.Abstract
The bovine production on pastures is a sustainable activity, responsible to generating income and employments in many tropical regions. However, in this system a major obstacle occurs during long dry periods, when the forages show low nutritional quality. In this research we evaluated the in vitro digestibility and degradability of Urochloa decumbens and Cynodon dactylons var Tiffton 85 inoculated with isolates of Aspergillus spp. from the gastrointestinal tract cattle. Four cows with rumen fistula were used as rumen contents donors to determine the in vitro digestibility of dry matter (IVDMD). The treatments were, as follows: control - No fungal inoculum, AT13 – (with Aspergillus terreus isolate), AF69 – (with Asperillus fumigates isolate, MIX (with mix of fungi AT13 and AF69). The analyses were performed in rumen simulator with four replications. The gas production was determined by semi-automatic method for both gas and the mechanics ability to fiber colonization of these fungi isolates were evaluated by scanning electron microscopy. The inclusion of AF69 and MIX promoted significant increase of IVDMD of U. decumbens (41.4 - 42.1%). The dry matter degradability were not influenced by the inoculums types using the by semi-automatic gas production method However, they linearly increased with fermentation time. The two isolates of Aspergillus spp. were able to colonize the U. decumbens fiber showing production of mycelium and reproductive structures in electronic microscopic analyses. The addition these Aspergillus isolates promotes significant increase of IVDMD to U. decumbens, indicating promising potential for development of microbial or probiotic additive to cattle raised on lignified tropical pasture.
References
Abrão, F. O., Duarte, E. R., Pessoa, M. S., Santos, V. L., Freitas Júnior, L. F, Barros K. O., Hughes A. F. S., Silva, T. D. & Rodriguez N. M. (2017). Notable fibrolytic enzyme production by Aspergillus spp. isolates from the gastrointestinal tract of beef cattle fed in lignied pastures. PLoS ONE, 128, 1-9. https://doi.org/10.1371/journal.pone.0183628
Abrão, F. O., Duarte, E. R., Rosa, C. A., Freitas, C. E. S., Vieira, E. A.& Huhges, A. F.S. (2014). Characterization of fungi from ruminal fluid of beef cattle with different ages and raised in tropical lignified pastures. Current Microbiology. 695, 649-659. https://doi: 10.1007/s00284-014-0633-5
Abrão, F. O., Duarte, E. R., Pessoa, M. S., Santos, L. V.& Rodrigues, N. M. (2018). Mycotoxicological safety and viability of Aspergillus spp.with probiotic potential from the bovine digestive tract. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 70, 833-1839. https://doi.org/10.1590/1678-4162-9853
Almeida P.N.M., Freitas C.E.S., Abrão F.O., Ribeiro I.C.O., Vieira E.A, Geraseev L.C.& Duarte E.R. (2014). Atividade celulolítica de fungos aerobios isolados do rúmen de bovinos leiteiros alimentados com forragens tropicais. Revista Caatinga, 274, 202-207.
Ankom, Technology (2014). Method 3: In vitro true digestibility using the DAISYII Incubator. Available in: (http://www.ankom.com/media/documents/ IVDMD_0805_D200.pdf.) [Accessed November 05,2014].
Chiou, P. W. S., Chen, C. R., Bi & Yu, B. (2002). Effects of Aspergillus oryzae fermentation extract on performance of lactating cows in the summer and winter in Taiwan. Asian-Austral. Journal Animal Science,15:382-389. https://doi.org/10.5713/ajas.2002.382
Curvelo, C. R. S., Rodrigues, R. A., Berger, P. G.& Rezende D.C. (2010). Microscopia eletrônica de varredura do processo infeccioso de Ramularia areola em folhas de algodoeiro. Tropical Plant Pathology, 352,108-113. https://doi.org/10.1590/S1982-56762010000200006
Dashtban, M., Schraft, H., Syed, T. A. & Qin, W. (2010). Fungal biodegradation and enzymatic modification of lignin. International Journal of Biochemistry and Molecular Biology.1:36-50. https://doi.org/10.1590/S1982-56762010000200006
Facchini, F. D. A., Vici, A. C., Reis, V. R. A., Jorge, J. A., Terenzi, H. F., Reis, R. A. & Polizeli, M.L.T.M. (2011). Production of fibrolytic enzymes by Aspergillus japonicas C03 using agro-industrial residues with potential application as additives in animal feed. Bioprocess and BiosysteDM Engineeringi, 34,347-355. . https://doi:10.1007/s00449-010-0477-8
Hartinger, T. & Zebeli, Q. (2021). The Present Role and New Potentials of Anaerobic Fungi in Ruminant Nutritionm. Journal Fungi, 3, 200. https://doi.org/10.3390/jof7030200
Holden, L.A. (1999). Comparison of methods of in vitro dry matter digestibility for ten feeds. Journal Dairy Science, 828,1791-1794. https://doi: 10.3168/jds.S0022-0302(99)75409-3.
Kamra, D.N. (2005). Rumen Microbial Ecosystem. Current Sience 89,125-135. https://doi:10.15406/BIJ.2017.01.00016
Lee, S. S., Ha, J. K. & Cheng, K. J. (2000). Influence of an anaerobic fungal culture administration on in vivo ruminal fermentation and nutrient digestion. Animal Feed Science and Technology, 88,201-217. https://doi10.1016/S0377-8401(00)00216-9
Liu, Y. S. , Baker, J. O., Zeng, Y., Himmel, M. E., Haas, T., Ding, S.Y. (2011).Cellobiohydrolase hydrolyzes crystalline cellulose on hydrophobic faces. Journal Biological Chemistry 286(13), 11195-11201. https://doi.org/ 10.1074/jbc.M110.216556.
Liu, K., Zhang, Y., Xu, Q., Zheng, N., Zhao, S., Huang, G & Wang, J. (2020). Ruminal microbiota-host interaction and its effect on nutrient metabolism. Animal Nutrition, 7, 49-55. https://doi.org/10.1016/j.aninu.2020.12.001
Moore, K. J. & Jung, H. J. ,G. (2001). Lignin and fiber digestion. Journal of Range Management, 54, 420-430. https://doi:10.2307/4003113
Paul, S. S., Kamra, D. N., Sastry, V. R. B., Sahu, N. P. & Agarwal, N. (2004). Effect of anaerobic fungi on in vitro feed digestion by mixed rumen microflora of buffalo. Reproduction, Nutrition Development. 44,313-319. https//doi.10.1051/rnd:2004036.
Russell, J.B. & Rychlik J.L. (2001). Factors that alter rumen microbial ecology. Science 11,1119-1122. . https//doi.10.1126/science.1058830.
Sobhy, M. A., Sallam, Mina, L. R., Abdelmalek, A. E., Kholif Soliman, M. Z., Mohamed, H. A., Hassan, S. Z., Marwa, F.A.A., Osama H.M & Olurotimi, A. O. (2020). The effect of Saccharomyces cerevisiae live cells and Aspergillus oryzae fermentation extract on the lactational performance of dairy cows. Animal Biotechnology, 31,6, 491-497. https//doi.10.1080/10495398.2019.1625783
Sirohi, S. K., Choudhury, P. K., Dagar, S. S., Puniy, A. K.& Singh D. (2012). Isolation, characterization and fibre degradation potential of anaerobic rumen fungi from cattle. Annals Microbiology. 1,1-10. https://doi.org/10.1007/s13213-012-0577-6
Tilley, J. M. A. & Terry R.A. (1963). A two-stage technique for in vitro digestion of forage crops. Journal of the British Grassland Society. 18, 104-111. https://doi:10.1111/j.1365-2494.1963.tb00335.x
Yang, W. Z., Beauchemin, K. A. & Rode, L. M. (2000). A comparison of methods of adding fibrolytic enzymes to lactating cow diets. Journal Dairy Sciense. 83, 2512-2520. https://doi.org/10.3168/jds.S0022-0302(00)75143-5
Zilio, E. M. C, Del Valle, T. A., Ghizzi, L. G., Takiya, C. S., Dias, M. S.,S, Nunes, A., Silva, G .G. & Rennó F.P. (2019). Effects of exogenous fibrolytic and amylolytic enzymes on ruminal fermentation and performance of mid-lactation dairy cows. Journal Dairy Sciense. 1025, 4179-4189. :https://doi.org/10.3168/jds.2018-14949
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Flávia Oliveira Abrão; Eduardo Robson Duarte; Moisés Sena Pessoa; Otaviano de Souza Pires Neto; Anna Carolynne Alvim Duque; Vera Lúcia dos Santos; Luciana Castro Geraseev; Eloísa de Oliveira Simões Simões; Norberto Mário Rodriguez
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.