Evaluation of antioxidant activity and toxicity in Artemia salina of the ether extract and fractions from Tecoma stans seeds

Authors

DOI:

https://doi.org/10.33448/rsd-v10i9.17829

Keywords:

Tecoma stans; Secondary metabolites; Antioxidant activity; Brine shrimp.

Abstract

Tecoma stans (L.) Juss. Ex Kunth is a species belonging to the Bignoniaceae family, popularly known as yellowing, yellow bell, and garden little ipe. Studies with T. stans seeds are scarce and it have potential in the search for natural compounds with biological activities. This study aimed to evaluate the toxicity and antioxidant activity of the ether extract (EE) and fractions from T. stans seeds. The EE was obtained in Soxhlet apparatus with petroleum ether and fractions were obtained by hydrolysis and esterification reactions. Phytochemical screening evaluated the presence of steroids, triterpenoids, alkaloids, coumarins and anthraquinones in EE. The toxicity was evaluated by the A. salina lethality test and antioxidant activity by DPPH method. It was observed the presence of steroids, triterpenoids and alkaloids in EE, which had no toxicity to A. salina (LC50 > 1000 µg/mL). The fractions of seeds exhibited toxicity on A. salina. All samples showed antioxidant activity with EC50 between 0.9 and 1.1 µg/mL. These results indicated potential applications for T. stans seeds.

References

Alonso-Castro, A. J., Zapata-Bustos, R., Romo-Yañez, J., Camarillo-Ledesma, P., Gómez-Sánchez, M. & Salazar-Olivo, L. A. (2010). The antidiabetic plants Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae) and Teucrium cubense Jacq (Lamiaceae) induce the incorporation of glucose in insulin-sensitive and insulin-resistant murine and human adipocytes. Journal of Ethnopharmacology, 127(1), 1-6. https://doi.org/10.1016/j.jep.2009.09.060

Amado, P. A., Ferraz, V., da Silva, D. B., Carollo, C. A., Castro, A. H. F. & Lima, L. A. R. S. (2018). Chemical composition, antioxidant and cytotoxic activities of extracts from the leaves of Smilax brasiliensis Sprengel (Smilacaceae). Natural Product Research, 32(5), 610-615. https://doi.org/10.1080/14786419.2017.1327861

Araújo, S. G., Pinto, M. E. A., Silva, N. L.; dos Santos, F. J. L., Castro, A. H. F. & Lima, L. A. R. S. (2013). Antioxidant and allelopathic activities of extract and fractions from Rosmarinus officinalis. Biochemistry and Biotechnology Reports, 2(1), 35-43. https://doi.org/10.5433/2316-5200.2013v2n1p35

Brand-Williams, W., Cuvelier, M. E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Cerqueira, F. M., de Medeiros, M. H. G. & Augusto, O. (2007). Dietetic antioxidants: controversies and perspectives. Química Nova, 30(2), 441-449. https://doi.org/10.1590/S0100-40422007000200036

Costantino, L., Raimondi, L., Pirisino, R., Brunetti, T., Pessotto, P., Giannessi, F., Lins, A. P., Barlocco, D., Antolini, L. & El-Abady, S. A. (2003). Isolation and pharmacological activities of the Tecoma stans alkaloids. Farmaco, 58(9), 781-785. https://doi.org/10.1016/S0014-827X(03)00133-2

Favilla, M., Macchia, L., Gallo, A. & Altomare, C. (2006). Toxicity assessment of metabolites of fungal biocontrol agent using two different (Artemia salina and Daphnia magna) invertebrate bioassays. Food and Chemical Toxicology, 44(11), 1922-1931. https://doi.org/10.1016/j.fct.2006.06.024

Finney, D. J. (1980). Probit analysis, a statistical treatment of the sigmoid response curve. University Press.

Gonçalves, T. P. R., Parreira, A. G. & Lima, L. A. R. S. (2020). Study of the antimicrobial activity of Tecoma stans (L.) ex Kunth (Bignoniaceae). Tchê Química, 18(35), 1037-1043.

Gonçalves, T. P. R. (2020). Estudo fitoquímico e avaliação do potencial antimicrobiano, sinérgico e antioxidante das flores de Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae). Master Dissertation, Federal University of São João del-Rei, Divinópolis, MG, Brazil.

Kranz, W. M. & Passini, T. (1997). Amarelinho: biologia e controle. Instituto Agronômico do Paraná.

Larbie, C., Nyarkoh, C. O. & Adjei, C.O. (2019). Phytochemical and safety evaluation of hydroethanolic leaf extract of Tecoma stans (L.) Juss. ex Kunth. Evidence-Based Complementary and Alternative Medicine, 2019, 1-12. https://doi.org/10.1155/2019/7417624

Lima, L. A. R. S. (2006). Estudo químico e avaliação biológica das sementes de Annona cornifolia A. St. -Hil. (Annonaceae). Doctoral Thesis, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

Lima, L. A. R. S., Alves, T. M. A., Zani, C. L., Pimenta, L. P. S. & Boaventura, M. A. D. (2012). Antioxidant and cytotoxic potential of fatty acid methyl esters from the seeds of Annona cornifolia A. St. -Hil. (Annonaceae). Food Research International, 48(2), 873-875. https://doi.org/10.1016/j.foodres.2012.07.016

Martin, C. A., de Almeida, V. V., Ruiz, M. R., Visentainer, J. E. L., Matshushita, M., de Souza, N. E. & Visentainer, J. V. (2006). Omega-3 and omega-6 polyunsaturated fatty acids: importance and occurrence in foods. Revista de Nutrição, 19(6), 761-770. https://doi.org/10.1590/S1415-52732006000600011

Marzouk, M. S. A., Gamal-Eldeen, A. M., Mohamed, M. A. & El-Sayed, M. M. (2006). Antioxidant and anti-proliferative active constituents of Tecoma stans against tumor cell lines. Natural Product Communications, 1(9), 735-743. https://doi.org/10.1177/1934578X0600100908

Matos, F. J. A. (2009). Introdução a fitoquímica experimental, Edições UFC.

Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. & McLaughlin, J. L. (1982). Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, 45(5), 31-34. https://doi.org/10.1055/s-2007-971236

Neha, K., Haider, M. R., Pathak, A. & Yar, M. S. (2019). Medicinal prospects of antioxidants: A review. European Journal of Medicinal Chemistry, 178, 687-704. https://doi.org/10.1016/j.ejmech.2019.06.010

Pereira, A. S., Shitsuka, D. M., Parreira, F. J. & Shitsuka, R. (2018). Metodologia da pesquisa científica[e-book]. UAB/NTE/UFSM. Avaliable: https://repositorio. ufsm. br/bitstream/handle.

Pimenta, L. P. S., Pinto, G. B., Takahashi, J. A., Silva, L. G. F. E. & Boaventura, M. A. D. (2003). Biological screening of Annonaceous Brazilian medicinal plants using Artemia salina (Brine Shrimp Test). Phytomedicine, 10(2-3), 209-212. https://doi.org/10.1078/094471103321659960

Pinto, M. E. A., Araújo, S. G., Morais, M. I., Sá, N. P., Lima, C. M., Rosa, C. A., Siqueira, E. P., Johann, S. & Lima, L. A. R. S. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciências, 89(3), 1671-1681. https://doi.org/10.1590/0001-3765201720160908

Pohanka, M. (2018). Oxidative stress in Alzheimer disease as a target for therapy. Bratislava Lek Listy, 119(9), 535-543. https://doi.org/10.4149/BLL_2018_097

Prasanna, V. L., Lakshman, K., Hegde, M. M. & Bhat, V. (2013). Antinociceptive and anti-inflammatory activity of Tecoma stans leaf extracts. Indian Journal of Research in Pharmacy and Biotechnology, 1(2), 156-160.

Salem, M. Z. M., Gohar, Y. M., Camacho, L. M., El-Shanhorey, N. A. & Salem, A. Z. M. (2013). Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. ex Kunth against nine species of pathogenic bacteria. African Journal of Microbiology Research, 7(5), 418-426. https://doi.org/10.5897/ajmr12.2274

Silva, V. C. B., Ribeiro-Neto, J. A., Alves, S. N. & Lima, L. A. R. S. (2015). Larvicidal activity of oils, fatty acids, and methyl esters from ripe and unripe fruit of Solanum lycocarpum (Solanaceae) against the vector Culex quinquefasciatus (Diptera: Culicidae). Revista da Sociedade Brasileira de Medicina Tropical, 48(5), 610-613. https://doi.org/10.1590/0037-8682-0049-2015

Simões, C. M. O., Schenkel, E. P., Gosmann, G., De Melo, J. C. P., Mentz, L. A. & Petrovick, P. R. (2007). Farmacognosia da planta ao medicamento, Editora da UFRGS.

Simopoulos, A. P. (2002). Omega-3 fatty acids in wild plants, nuts and seeds. Asia Pacific Journal of Clinical Nutrition, 11(S6), S163-S173. https://doi.org/10.1046/j.1440-6047.11.s.6.5.x

Thein, A. M. & Oo, K. C. C. (2019). Phytochemical investigation of Tecoma stans (L.) H. B. K. leaves and its brine shrimp lethality test. 2nd Myanmar Korea Conference Research Journal, 1, 1-11.

Downloads

Published

22/07/2021

How to Cite

AZEVEDO, L. S.; GONÇALVES, T. P. R.; AMADO, P. A.; LIMA, L. A. R. dos S. Evaluation of antioxidant activity and toxicity in Artemia salina of the ether extract and fractions from Tecoma stans seeds . Research, Society and Development, [S. l.], v. 10, n. 9, p. e12510917829, 2021. DOI: 10.33448/rsd-v10i9.17829. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17829. Acesso em: 25 sep. 2021.

Issue

Section

Agrarian and Biological Sciences