Antimicrobial activity of methanol extracts from different species of the genus Lippia
DOI:
https://doi.org/10.33448/rsd-v10i9.18051Keywords:
Lippia; Antimicrobials; Extracts.Abstract
The species of the genus Lippia (Verbenaceae) have several bioactive molecules from their secondary metabolism, which have antimicrobial, analgesic, antifungal and immunomodulatory activities. This study aimed to evaluate the in vitro antimicrobial activity of methanol extract of L. alnifolia, L. origanoides, L. insignis and L. thymoides leaves, against clinically important microorganisms. A phytochemical screening of plant extracts was carried out and the antimicrobial activity was evaluated by the disk diffusion method, in addition to the determination of the minimum inhibitory concentration (MIC) against the bacteria Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus Salmonella choleraesuis, and the fungi Candida albicans and Candida glabrata. The presence of saponins, terpenes, steroids, coumarins, phenolic acids and flavonoids was observed in the phytochemical evaluation. Methanolic extracts from the leaves of L. alnifolia, L. origanoides and L. insignis showed antimicrobial activity against all tested microorganisms. The methanol extract of L. thymoides showed antimicrobial activity against the microorganisms M. luteus, P. aeruginosa, S. aureus, C. albicans and C. glabrata. Most extracts showed bacteriostatic activity and all extracts showed fungistatic activity for C. albicans and fungicide for C. glabrata. The use of Lippia extracts can be a viable alternative in the treatment of bacterial and fungal infectious diseases.
References
Barreto, H. M., Lima, I. S., Coelho, K. M. R. N., Osório, L. Â. R., Mourão, R. A., Santos, B. H. C., Coutinho, H. D. M., Abreu, A. P. L., Medeiros, M. G. F., Citó, A. M. G. L., & Lopes, J. A. D. (2014). Effect of Lippia origanoides H.B.K. essential oil in the resistance to aminoglycosides in methicillin resistant Staphylococcus aureus. Eur J Integr Med, 6, 560-564.
Botelho, M. A., Barros, G., Queiroz, D. B., Carvalho, C. F., Gouvea, J., Patrus, L., Bannet, M., Patrus, D., Rego, A., Silva, I., Campus, G., & Araújo-Filho, I. (2016). Nanotechnology in phytotherapy: antiinflammatory effect of a nanostructured thymol gel from Lippia sidoides in acute periodontitis in rats. Phytother Res, 30, 152-159.
Botelho, M. A., Nogueira, N. A. P., Bastos, G. M., Fonseca, S. G. C., Lemos, T. L.G., Matos, F. J. A., Montenegro, D., Heukelbach, J., Rao, V. S., & Brito, G. A. C. (2007). Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol Res, 40, 349-356.
Botelho, M. A., Rao, V. S., Montenegro, D., Bandeira, M. A. M., Fonseca, S. G. C., Nogueira, N. A. P., Ribeiro, R. A., & Brito, G. A. C. (2008). Effects of a herbal gel containing carvacrol and chalcones on alveolar bone resorption in rats on experimental periodontitis. Phytother Res, 22, 442-449.
Brasil. (2009). Relação nacional de plantas medicinais de interesse ao SUS. Ministério da Saúde.
Clinical and Laboratory Standards Institute. (2005). Performance standards for antimicrobial susceptibility testing, fifteenth informational supplement. CLSI document M100-S15. Wayne, Pensylvania: CLSI.
Costa, J. G. M., Rodrigues, F. F. G., Angélico, E. C., Silva, M. R., Mota, M. L., Santos, N. K. A., & Lemos, T. L. G. (2005). Estudo químico-biológico dos óleos essenciais de Hyptis martiusii, Lippia sidoides e Syzigium aromaticum frente às larvas do Aedes aegypti. Rev Bras Farmacogn, 15(4), 304-309.
Costa, P. S., Souza, E. B., Brito, E. H. S., & Fontenelle, R. O. S. (2017). Atividade antimicrobiana e potencial terapêutico do gênero Lippia sensu lato (Verbenaceae). Hoehnea, 44(2), 158-171.
Gobbo-Neto, L., & Lopes, N. P. (2007). Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Quim Nova, 30(2), 374-381.
Hirota, T., Lee, J. W., Lewis, W. G., Zhang, E. E., Breton, G., Liu, X., Garcia, M., Peters, E. C., Etchegaray, J. P., Traver, D., Schultz, P. G., & Kay, S. A. (2010). High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIα as a clock regulatory kinase. PLoS Biol, 8(12), 1-14.
Juiz, P. J. L., Alves, R. J. C., & Barros, T. F. (2010). Uso de produtos naturais como coadjuvante no tratamento da doença periodontal. Rev Bras Farmacogn, 20(1), 134-139.
Juiz, P. J. L., Lucchese, A. M., Gambari, R., Piva, R., Penolazzi, L., Di Ciano, M., Uetanabaro, A. P. T., Silva, F., & Avila-Campos, M. J. (2015). Essential oils and isolated compounds from Lippia alba leaves and flowers: antimicrobial activity and osteoclast apoptosis. Int J Mol Med, 35(1), 211-217.
Leal, A. L. A. B., Braga, A. L., Barbosa, B. A., Rodrigues, A. M. X., Teixeira, F. A. F. C., Silva, K. M. R., Júnio, E. C., Leite, T. R. S., & Amorim, M. S. (2019). Antimicrobial action of essential oil of Lippia origanoides H.B.K. J Clin Microbiol Biochem Technol, 5(1), 07-12.
Leyva-Jiménez, F. J., Lozano-Sánchez, J., Cádiz-Gurrea, M. L., Arráez-Román, D., & Segura-Carretero, A. (2019). Functional ingredients based on nutritional phenolics. A case study against inflammation: Lippia genus. Nutrients, 11(1646), 1-24.
Marques, G. S., Silva, C. C. A. R., Vilela, W. T., Figueirêdo, C. B. M., Silva, A. C. A. F., Silva, R. M. F., & Neto, P. J. R. (2015). Plantas medicinais como alternativa terapêutica para aumento da resistência imunológica. Rev Ciênc Farm Básica Apl, 36(1), 27-33.
Martinelli, G., & Moraes, M. A. (2013). Livro vermelho da flora do Brasil.: Andrea Jakobsson: Instituto de Pesquisas Jardim Botânico do Rio de Janeiro.
Matos, F. J. A. (1997). Introdução à Fitoquímica Experimental. Edições UFC.
Meira, A. L. T., Todescan, S. M. C., Azoubel, E., Bittencourt, S., & Azoubel, M. C. F. (2007). Uso de antimicrobianos locais em periodontia: uma abordagem crítica. Periodontia, 17(1), 92-98.
National Committee For Clinical Laboratory Standards. (2003). Performance standards for antimicrobial disk susceptibility tests, approved standard - eighth edition. NCCLS document M2-NCCLS. Wayne, Pennsylvania: NCCLS.
Nunes-Pinheiro, D. C. S., Leite, A. K. R. M., Farias, V. M., Braga, L. T., & Lopes, C. A. P. (2003). Atividade imunomoduladora das plantas medicinais: perspectivas em medicina veterinária. Ciência Anim, 13(1), 23-32.
Okhale, S. E., Nwanosike, E. M., Fatokun, O. T., & Kunle, O. F. (2016). Phytochemistry and ethnopharmacology of Lippia genus with a statement on chemotaxonomy and essential oil chemotypes. Int J Pharmacogn, 3(5), 201-211.
Oliveira, A. R. M. F., Oliveira, L. M., Carneiro, J. S., Silva, T. R. S., & Costa, L. C. B. (2018). Leaf anatomy and essential oil production of Lippia native species. Brazilian J Agric, 93(3), 324-335.
Oliveira, D. R., Leitão, G. G., Fernandes, P. D., & Leitão, S. G. (2014). Ethnopharmacological studies of Lippia origanoides. Rev Bras Farmacogn, 24, 206-214.
Palaska, I., Papathanasiou, E., & Theoharides, T. C. (2013). Use of polyphenols in periodontal inflammation. Eur J Pharmacol, 720, 77-83.
Pascual, M. E., Slowing, K., Carretero, E., Sánchez Mata, D., & Villar, A. (2001). Lippia: traditional uses, chemistry and pharmacology: a review. J Ethnopharmacol, 76(3), 201-214.
Perera, W. H., Bizzo, H. R., Gama, P. E., Alviano, C. S., Salimena, F. R. G., Alviano, D. S., & Leitão, S. G. (2017). Essential oil constituents from high altitude Brazilian species with antimicrobial activity: Baccharis parvidentata Malag., Hyptis monticola Mart. ex Benth. and Lippia origanoides Kunth. JEOR, 29(2), 109-116.
Pinto, C. P., Rodrigues, V. D., Pinto, F. P., Pinto, R. P., Uetanabaro, A. P. T., Pinheiro, C. S. R., Gadea, S. F. M., Silva, T. R. S., & Lucchese, A. M. (2013). Antimicrobial activity of Lippia species from the Brazilian semiarid region traditionally used as antiseptic and anti-infective agents. Evid Based Complement Alternat Med, 2013, 1-5.
Queiroz, M. R. A., Almeida, A. C., Andrade, V. A., Lima, T. S., Martins, E. R., Figueiredo, L. S., & Careli, R. T. (2014). Avaliação da atividade antibacteriana do óleo essencial de Lippia origanoides frente à Staphylococcus sp. isolados de alimentos de origem animal. Rev Bras Pl Med, 16(3), 737-743.
Rodrigues, Í. S. C., Tavares, V. N., Pereira, S. L. S., & Costa, F. N. (2009). Antiplaque and antigingivitis effect of Lippia sidoides. A double-blind clinical study in humans. J Appl Oral Sci, 17(5), 404-407.
Rozza, A. L., Moraes, T. M., Kushima, H., Tanimoto, A., Marques, M. O. M., Bauab, T. M., Hiruma-Lima, C. A., & Pellizzon, C. H. (2011). Gastroprotective mechanisms of Citrus lemon (Rutaceae) essential oil and its majority compounds limonene and β-pinene: involvement of heat-shock protein-70, vasoactive intestinal peptide, glutathione, sulfhydryl compounds, nitric oxide and prostaglandin E2. Chemico-Biological Interactions, 189, 82-89.
Santos, N. O., Pascon, R. C., Vallim, M. A., Figueiredo, C. R., Soares, M. G., Lago, J. H. G., & Sartorelli, P. (2016). Cytotoxic and antimicrobial constituents from the essential oil of Lippia alba (Verbenaceae). Medicines, 3(22), 1-9.
Sarrazin, S. L. F., Silva, L. A., Oliveira, R. B., Raposo, J. D. A., Silva, J. K. R., Salimena, F. R. G., Maia, J. G. S., & Mourão, R. H. V. (2015). Antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from Lippia origanoides Kunth. Lipids Health Dis, 14(145), 1-8.
Silva, F. S., Menezes, P. M. N., Sá, P. G. S., Oliveira, A. L. S., Souza, E. A. A., Bamberg, V. M., Oliveira, H. R., Oliveira, S. A., & Araújo, R. E., Uetanabaro, A. P. T., Silva, T. R. S., Almeida, J. R. G. S. & Lucchese, A. M. (2015). Pharmacological basis for traditional use of the Lippia thymoides. Evid Based Complement Alternat Med, 2015, 1-10.
Silva, F. S., Menezes, P. M. N., Sá, P. G. S., Oliveira, A. L. S., Souza, E. A. A., Almeida, J. R. G. S., Lima, J. T., Uetanabaro, A. P. T., Silva, T. R. S., Peralta, E. D., & Lucchese, A. M. (2016). Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. Pharm Biol, 54(1), 25-34.
Soares, B. V., & Tavares-Dias, M. (2013). Espécies de Lippia (Verbenaceae), seu potencial bioativo e importância na Medicina Veterinária e Aquicultura. Biota Amaz, 3(1), 109-123.
Tavares, L. S., Souza, V. C., Nunes, V. S., Silva, O. N., Souza, G. T., Marques, L. F., Goliatt, P. V. Z. C., Viccini, L. F., Franco, O. L., & Santos, M. O. (2020). Antimicrobial peptide selection from Lippia spp leaf transcriptomes. Peptides, 129, 1-8.
Vilela, D. A. D., Silva, B. A. O., Brito, M. C., Menezes, P. M. N., Bomfim, H. F., Duarte-Filho, L. A. M. S., Silva, T. R. S., Ribeiro, L. A. A., Lucchese, A. M., & Silva, F. S. (2020). Lippia alnifolia essential oil induces relaxation on Guinea-pig trachea by multiple pathways. J Ethnopharmacol, 246, 1-10.
Wagner, H., & Bladt, S. (1995). Plant Drug Analysis: a thin layer chromatography atlas. Springer Verlag.
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Copyright (c) 2021 Soraya Castro Trindade; Jurandi Nery Pereira-Filho; Laerte Oliveira Barreto Neto; Thais Brito de Oliveira Moura; Antonio Pedro Fróes de Farias; José Tadeu Raynal Rocha Filho; Rebeca Pereira Bulhosa Santos; Acsa Oliveira Magalhães; Ellen Karla Nobre dos Santos-Lima; Paulo Cirino Carvalho-Filho; Vera Lúcia Costa Vale; Angélica Maria Lucchese
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