Análise de proteínas salivares de corredores de rua após evento esportivo real

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.18183

Keywords:

Mass Spectrometry; Exercise; Proteomics; Saliva.

Abstract

The aim was to characterize acute exercise-induced changes in salivary proteins of street runners. Saliva samples from 12 adult male athletes were collected before and immediately after a street race. Two groups were formed based on the distance covered, 5 km (n=4) and 10 km (n=8). Samples were subjected to depletion of amylase, albumin and immunoglobulin G. Then they were concentrated, digested and analyzed by nano-UPLC-tandem nano-ESI-MSE. A total of 69 proteins were identified. Significant changes were observed in the expression of 15 proteins in the 5 km group and 13 proteins in the 10 km group. Among the proteins with altered expression, only 7 had already been described in the literature in similar models (Alpha-Amylase 1, Lactoperoxidase, Alpha Skeletal Muscle Actin, Cystatin-B, Cystatin- SN, Cystatin- SA and Androgen-regulated Protein 3B in the submaxillary gland). This study has shown that street running induces acute changes in the salivary proteome. The results obtained add to the limited data available in the literature in the search for a better understanding of the acute effects of exercise.

Author Biographies

Cícero Matheus Lima Amaral, Universidade Estadual do Ceará

Bachelor of nutrition. State University of Ceará, Health Sciences Center, Nutrition Course. Fortaleza, Ceará, Brazil

Iago Almeida da Ponte, Universidade Estadual do Ceará

Graduating in Nutrition. State University of Ceará, Health Sciences Center, Nutrition Course. Fortaleza, Ceará, Brazil.

Géssica de Souza Martins, Universidade Estadual do Ceará

Graduating in Nutrition. Fametro University Center, Nutrition Course. Fortaleza, Ceará, Brazil.

Daniel Freire Lima, Universidade Estadual do Ceará

Bachelor of Nutrition. State University of Ceará, Health Sciences Center, Nutrition Course. Fortaleza, Ceará, Brazil

Abelardo Barbosa Moreira Lima Neto, Universidade Estadual do Ceará

PhD in Biotechnology. Maurício de Nassau University Center, Health Sciences Center, Nutrition Course. Fortaleza, Ceará, Brazil

Ana Cristina Monteiro Moreira, Universidade Estadual do Ceará

PhD in Biochemistry. University of Fortaleza, Health Sciences Center, Pharmacy Course. Fortaleza, Ceará, Brazil.

Maria Izabel Florindo Guedes, Universidade Estadual do Ceará

PhD in Biochemistry. State University of Ceará, Health Sciences Center, Nutrition Course. Fortaleza, Ceará, Brazil

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06/08/2021

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AMARAL, C. M. L.; PONTE, I. A. da .; MARTINS, G. de S.; LIMA, D. F.; LIMA NETO, A. B. M.; MONTEIRO MOREIRA, A. C. .; GUEDES, M. I. F. Análise de proteínas salivares de corredores de rua após evento esportivo real . Research, Society and Development, [S. l.], v. 10, n. 10, p. e114101018183, 2021. DOI: 10.33448/rsd-v10i10.18183. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18183. Acesso em: 18 oct. 2021.

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Health Sciences