Evaluation of genetic diversity of Cryptococcus neoformans samples isolated in different geographical regions
DOI:
https://doi.org/10.33448/rsd-v10i9.18333Keywords:
Cryptococcus neoformans; Phylogeny; Recombination genetic.Abstract
Cryptococcus neoformans is the etiological agent of cryptococcosis, an opportunistic fungal infection and frequently occurs in immunocompromised patients, with an underlying disease, mainly affecting the central nervous system and lungs. Analysis of genomic sequences of microorganisms through phylogenies has demonstrated a wide spectrum of applications in scientific research and medicine. In the health area, the use of this tool contributes to the diagnosis and consequently to the development of programs to improve the treatment and control of diseases. Furthermore, the phylogenetic analysis is important to characterize new species that can development possible genetic recombinations, which in most cases is responsible for the resistance of pathogens to the treatment. Thus, this study aimed to analyze the genetic variability of 18S and 28S genes of C. neoformans in different geographic regions. Were selected 191 sequences of the 18S gene and 132 sequences of the 28S gene to carry out phylogenetic and split analyses. The results demonstrated that the 28S gene has more genetic variability when compared to the 18S gene, which has been shown to be more conserved. This variability can be a result from genetic recombination and migration of different strains from one geographic region to another through the tourism of individuals with the fungal. Additionally, in the future, this genetic recombination can result in a new species capable of causing diseases in humans.
References
Aguiar, P. A. D. F. D., Pedroso, R. D. S., Borges, A. S., Moreira, T. D. A., Araújo, L. B. D., & Röder, D. V. D. D. B. (2017). The epidemiology of cryptococcosis and the characterization of Cryptococcus neoformans isolated in a Brazilian University Hospital. Revista do Instituto de Medicina Tropical de São Paulo, 59.
Ajawatanawong, P. (2016). Molecular phylogenetics: Concepts for a newcomer. Network Biology, 185-196.
Araújo, E. C., Táparo, C. V., Uchida, C. Y., & Marinho, M. (2015). Cryptococcus: isolamento ambiental e caracterização bioquímica. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 67, 1003-1008.
Arechavala, A., Negroni, R., Messina, F., Romero, M., Marín, E., Depardo, R., ... & Santiso, G. (2018). Criptococose em Hospital de Doenças Infecciosas de Buenos Aires, Argentina. Revisão de 2.041 casos: Diagnóstico, aspectos clínicos e terapêutica. Revista iberoamericana de micologia , 35 (1), 1-10.
Ashton, P. M., Thanh, L. T., Trieu, P. H., Van Anh, D., Trinh, N. M., Beardsley, J., ... & Day, J. N. (2019). Three phylogenetic groups have driven the recent population expansion of Cryptococcus neoformans. Nature communications, 10(1), 1-10.
Caldart, E. T., Mata, H., Canal, C. W., & Ravazzolo, A. P. (2016). Análise filogenética: conceitos básicos e suas utilizações como ferramenta para virologia e epidemiologia molecular. Acta Scientiae Veterinariae, 44, 1-20.
Costa, A. K., Sidrim, J. J., Cordeiro, R. A., Brilhante, R. S., Monteiro, A. J., & Rocha, M. F. (2010). Urban pigeons (Columba livia) as a potential source of pathogenic yeasts: a focus on antifungal susceptibility of Cryptococcus strains in Northeast Brazil. Mycopathologia, 169(3), 207-213.
Desnos-Ollivier, M., Patel, S., Spaulding, AR, Charlier, C., Garcia-Hermoso, D., Nielsen, K., & Dromer, F. (2010). Infecções mistas e evolução in vivo no fungo patógeno humano Cryptococcus neoformans. MBio, 1 (1), e00091-10.
Faganello, J. (2008). Estudo da variabilidade e diferenças morfológicas entre as espécies Cryptococcus neoformans e Cryptococcus gatii por análise de diferença representacional e microscopia eletrônica de varredura (Tese de Doutorado). Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil.
Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. evolution, 39(4), 783-791.
Ferreira-Paim, K., Ferreira, T. B., Andrade-Silva, L., Mora, D. J., Springer, D. J., Heitman, J., ... & Silva-Vergara, M. L. (2014). Phylogenetic analysis of phenotypically characterized Cryptococcus laurentii isolates reveals high frequency of cryptic species. PLoS One, 9(9), e108633.
Ferreira-Paim, K., Andrade-Silva, L., Fonseca, F. M., Ferreira, T. B., Mora, D. J., Andrade-Silva, J., ... & Silva-Vergara, M. L. (2017). MLST-based population genetic analysis in a global context reveals clonality amongst Cryptococcus neoformans var. grubii VNI isolates from HIV patients in Southeastern Brazil. PLoS neglected tropical diseases, 11(1), e0005223.
Galiza, G. J., Silva, T. M., Caprioli, R. A., Tochetto, C., Rosa, F. B., Fighera, R. A., & Kommers, G. D. (2014). Determinação das características histomorfológicas e histoquímicas no diagnóstico da criptococose em animais de companhia. Pesquisa Veterinária Brasileira , 34 (3), 261-269.
Hagen, F., Khayhan, K., Theelen, B., Kolecka, A., Polacheck, I., Sionov, E., ... & Boekhout, T. (2015). Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genetics and Biology, 78, 16-48.
Meyer, W., Aanensen, D. M., Boekhout, T., Cogliati, M., Diaz, M. R., Esposto, M. C., ... & Kwon-Chung, J. (2009). Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Medical mycology, 47(6), 561-570.
Moreira, T. D. A., Ferreira, M. S., Ribas, R. M., & Borges, A. S. (2006). Criptococose: estudo clínico-epidemiológico, laboratorial e das variedades do fungo em 96 pacientes. Revista da Sociedade Brasileira de Medicina Tropical, 39, 255-258.
Nascimento, M. A., Santos, E. C. M., de Carvalho, V. M., Borges, M. S., Ederli, J. P. B., de Albuquerque, C. A. N., ... & Moris, D. V. (2020). Perfil de pacientes com criptococose em hospital regional do interior de São Paulo. Research, Society and Development, 9(9), e598997642-e598997642.
O'Meara, T. R., Xu, W., Selvig, K. M., O'Meara, M. J., Mitchell, A. P., & Alspaugh, J. A. (2014). The Cryptococcus neoformans Rim101 transcription factor directly regulates genes required for adaptation to the host. Molecular and Cellular Biology, 34(4), 673-684.
Pinheiro, M. C., Dos Reis, D. S. T., de Brito, M. T. F. M., & Quaresma, J. A. S. (2019). Criptococose na Amazônia: uma visão geral atual e perspectivas futuras. Acta tropica , 197 , 105023.
Pizani, A. T., & dos Santos, M. O. (2017). Criptococose em pacientes HIV positivos: revisão sistemática da literatura. Revista Saúde UniToledo, 1(1).
Reolon, A., Perez, L. R. R., & Mezzari, A. (2004). Prevalência de Cryptococcus neoformans nos pombos urbanos da cidade de Porto Alegre, Rio Grande do Sul. Jornal Brasileiro de Patologia e Medicina Laboratorial, 40, 293-298.
Rocha, J. É. L., Sousa, R. D. S., & Fonseca, F. M. (2016). DIVERSIDADE GENÉTICA DE ISOLADOS DE Sporothrix schenckii EM PAÍSES DO CONTINENTE AMERICANO. REVISTA UNINGÁ REVIEW, 27(3).
Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.
Santos, G. D. R., Dias, I. C. D. R., Ventura, C. A., & Fonseca, F. M. (2016). VARIABILIDADE GENÉTICA DE ISOLADOS BRASILEIROS DE Histoplasma capsulatum. REVISTA UNINGÁ REVIEW, 28(2).
Severo, C. B., Gazzoni, A. F., & Severo, L. C. (2009). Capítulo 3: criptococose pulmonar. Jornal Brasileiro de Pneumologia, 35(11), 1136-1144.
Silva, S. P., Costa, C. B. L., Silva, J. D. F., Alves, R. R. V., Silva, G. A. S., Freitas, A. F. S, ... & Napoleão, T. H. (2021). Mecanismos de resistência de Cryptococcus spp. e compostos de plantas como ferramentas para combatê-los. Research, Society and Development , 10 (2), e57810212819-e57810212819.
Sloan, D. J., & Parris, V. (2014). Cryptococcal meningitis: epidemiology and therapeutic options. Clinical epidemiology, 6, 169.
Srikanta, D., Santiago‐Tirado, F. H., & Doering, T. L. (2014). Cryptococcus neoformans: historical curiosity to modern pathogen. Yeast, 31(2), 47-60.
Souza, J. A. M. D. O. (2018). Variabilidade genética de Cryptococcus ambientais na cidade do Salvador–BA (Dissertação de Mestrado). Universidade Federal da Bahia, Salvador, Bahia, Brasil.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology and evolution, 28(10), 2731-2739.
Trilles, L., Lazéra, M. D. S., Wanke, B., Oliveira, R. V., Barbosa, G. G., Nishikawa, M. M., ... & Meyer, W. (2008). Regional pattern of the molecular types of Cryptococcus neoformans and Cryptococcus gattii in Brazil. Memórias do Instituto Oswaldo Cruz, 103, 455-462.
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Copyright (c) 2021 Dakson Douglas Araújo; Raí Emanuel da Silva; Higinalice da Silva Pereira; Dacylla Sampaio Costa; Rodrigo Elísio de Sá; Antonio Rodrigues da Silva Neto; Nathanael dos Santos Alves; Fernanda Machado Fonseca
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