Fermentation of elephant grass inoculated with lactic acid bacteria from the digestive tract of cattle
DOI:
https://doi.org/10.33448/rsd-v10i10.18813Keywords:
Cameroon; Enterobacteriaceae; Inoculation; Lactobacillus plantarum; Probiotics.Abstract
The objective of this work was to evaluate the effects of inoculation of a strain of lactic bacteria from the digestive tract of cattle on the fermentation of elephant grass (Pennisetum purpureum cv. Cameroon). The experimental design was completely randomized with six replications of the of the following experimental groups: (CONT) Control without additive, (LACT5) Lactobacillus plantarum at Colony Forming Units per gram (CFU/g) of forage, (LACT9) L. plantarum at 1.0 × 109 CFU / g of forage. Losses of dry matter and effluent and pH after 31 days of fermentation at 37 °C were not influenced by the inclusion of the bacteria. The experimental groups did not vary their populations of lacti bacteria, yeast and mycelial fungi. However, the inoculation of this lactic bacterium reduces the population of enterobacteria and does not allow the growth of those non-fermenters of lactose. These results point to a promising potential for using this strain to reduce these pathogens in elephant grass silage.
References
Alvares, C. A., Stape, J. L., Sentelhas, P. C., Gonc¸ Alves, J. L. M., & Sparovek, G. (2013). Köppen’s climate classification map for Brazil. Meteorol. Z 22, 711-728.
Borreani, G., Tabacco, E., Schmidt, R. J., Holmes, B. J., & Muck, R. E. (2018). Silage review: Factors affecting dry matter and quality losses in silages. Journal of Dairy Science, 101(5), 3952-3979. https://doi.org/10.3168/jds.2017-13837
Bumbieris, J. V. H., Oliveira, M. R., Barbosa, M. A. A. F., & Jobim, C.C. (2011). Use of winter cultures for forage conservation. International Syposium on Forage Quality and Conservation. 2, 65-84.
Daniel, J. L. P., Bernardes, T. F., Jobim, C. C, Schmidt, P., & Nussio, L. G. (2019). Production and utilization of silages in tropical areas with focus on Brazil. Grass and Forage Science, 74(2), 188-200. https://doi.org/10.1111/gfs.12417
Driehuis, F. (2011). Ocorrência de micotoxinas na silagem. In: II Simpósio Internacional of Forage Quality and Conservation, São Pedro, Anais. São Pedro: Simpósio Internacional of Forage Quality and Conservation, 85-104.
Dugan, F. M. (2006). The identification of fungi: an illustrated introduction with keys, glossary, and guide to literature. St. Paul, Minn.: American Phytopathological Society, 176.
Evangelista, A. R., Siqueira, G. R., Lima, J. Á., Lopes, J., & Rezende, A. V. (2009). Alterações bromatológicas e fermentativas durante o armazenamento de silagens de cana-de-açúcar com e sem milho desintegrado com palha e sabugo. Revista Brasileira de Zootecnia, 38, 20-26. doi:10.1590/S1516-3598200900010000
Ferreira, D. J., Lana, R. P., Zanine, A. M, Santos, E. M., Veloso, C. M., & Ribeiro, G. A. (2013). Silage fermentation and chemical composition of elephant grass inoculated with rumen strains of Streptococcus bovis. Animal Feed Science and Technology, 183, 22-28. https://doi.org/10.1016/j.anifeedsci.2013.04.020
Ferreira, D. J., Zanine, A. M., Lana, R. P., Ribeiro, M. D., Alves, G. R., & Mantovani, H. C. (2014). Chemical composition and nutrient degradability in elephant grass silage inoculated with Streptococcus bovisis olated from the rumen. In: Anais da Academia Brasileira de Ciências, 86, 465-474. https://doi.org/10.1590/0001-37652014112312
Guimarães, C. G., Bonfá, C. S., Evangelista, A. R., Santos, A. S. D., Pantoja, L. D. A., & Castro, G. H. D. F. (2018). Fermentation characteristics of elephant grass silages with macaúba cake. Acta Scientiarum. Animal Sciences, on-line, 40. doi:10.4025/actascianimsci.v40i1.42523
Lane D. J. (1991). 16S/23S rRNA sequencing In: E Stackebrandt and M Good fellow, ed Nucleic Acid Techniques in Bacterial Systematics. Chichester: Wiley. 115-175.
Maijala R., Ranta J., & Seuna E. (2005)., The efficiency of the Finnish Salmonella Control Programme. Food Control, 16(8), 669-675. Disponível em: https://doi.org/10.1016/j.foodcont.2004.06.003
Mcdonald, P., Henderson, A. R., & Heron. S. J. E. (1991). The biochemistry of silage. New York: JohnWiley, 226.
Murray, P., Baron, E. J., Jorgensen, J. H., Pfaller, M. A., & Yolken, R. H. (2007). Manual of Clinical Microbiology. 9th ed. American Society for Microbiology, Washington, DC, 2488.
Nussio, L. G. (2005). Silage production from tropical forages. In R. S. Park, M. D. Stronge (Eds.), Proceedings of the XIV International Silage Conference, Belfast, UK, Anais. Belfast, UK: Proceedings of the XIV International Silage Conference, 97-107.
Renhe, I. R. T., Volp, A., Vidigal, J., & Stringheta, P. (2006). Monascus na nutrição e saúde. Brazilian Jornal of Food and Nutrition, 17(3), 337-343.
Ribeiro, L., Pires, A. J. V., Carvalho, G. G. P. De, Pereira, M., Santos, A. B. dos, & Rocha, L. C. (2014). Características fermentativas, composição química e fracionamento de carboidratos e proteínas de silagem de capim-elefante emurchecido ou com adição de torta de mamona. Semina: Ciências Agrárias, 35, 1447-1462. doi:10.5433/1679-0359.2014v35n3p1447
Silva, D. J., & Queiroz, A. C. (2002). Análise de Alimentos: métodos químicos e biológicos. 3.ed. Viçosa: UFV, 235. Revista Ciência Agronômica, 41, 482-489. doi:10.1590/S1806-66902010000300023
Silva, S., & Xará, S. (2013). Levedura de arroz vermelho no tratamento da hipercolesterolemia. Revista Nutrícias, (19), 24-27.
Tomaz, P. K., Araujo, L. C. De, Sanches, L. A., Araujo, S. N. S. Dos, De Lima, T. O., Lino, A. D. A., & Ferreira, E. M. (2018). Effect of sward height on the fermentability coefficient and chemical composition of Guinea grass silage. Grass and Forage Science, 73, 588-598. https://doi.org/10.1111/gfs.12349
Veloso, V. A. (2016). Bactérias láticas com potencial probiótico provenientes de bezerros leiteiros desmamados criados no semiárido. Monografia, Instituto de Ciências Agrárias da UFMG.
Zhang, Y. C., LI, D. X., Wang, X. K., Lin, Y. L., Zhang, Q., Chen, X. Y., & Yang, F. Y. (2019). Fermentation quality and aerobic stability of mulberry silage prepared with lactic acid bacteria and propionic acid. Animal Science Journal. 90(4), 513-522. https://doi.org/10.1111/asj.13181
Yuan, X., Guo, G., Wen, A., Desta, S. T., Wang, J., Wang, Y., & Shao, T. (2015). The effect of different additives on the fermentation quality, in vitro digestibility and aerobic stability of a total mixed ration silage. Animal Feed Science and Technology, 207, 41-50. https://doi.org/10.1016/j.anifeedsci.2015.06.001
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Copyright (c) 2021 Aniele de Cássia Rodrigues Veloso; Thiago Alves Xavier dos Santos; Lavínia Francine Xavier Santos; Valdo Soares Martins Júnior; Suze Adriane Fonseca; Abigair Duarte Matias ; Rafael Bolina Silva; Victor Alugustus Vasconcelos de Oliveira; Thiago Gomes dos Santos Braz; Eduardo Robson Duarte
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