Detection of Campylobacter jejuni, Campylobacter coli, and virulence genes in poultry products marketed in Northeastern Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.19224

Keywords:

Campylobacter; Broiler chickens; Microbiological control; PCR.

Abstract

In this study, we evaluated the prevalence of Campylobacter jejuni, Campylobcater coli, and virulence genes in fresh, chilled, and frozen chicken carcasses with livers and gizzards sold in public markets and supermarkets. Of the 90 samples analyzed, C. jejuni was the most prevalent, with 28.8% of positive samples, whereas C. coli was positive in 15.6% of samples. In public market samples, C. coli had a higher prevalence than C. jejuni, with 16.7% positive samples detected, whereas in supermarket samples, C. jejuni was more prevalent (36.7% positivity). C. jejuni was detected in all forms of commercialized carcasses; however, there was a higher prevalence (43.3%) in chilled samples than C. coli, which was not detected in frozen samples but showed a higher prevalence (16.7%) in fresh samples. Both species were detected in different poultry products, with C. jejuni being more prevalent (53.3%) in liver samples. C. coli showed a higher prevalence in samples of meat pieces (10%). The presence of five virulence genes related to adherence (Peb1, JlpA, CadF, and CapA) and invasion (CiaB) was also observed in both species.

Author Biographies

Felipe Pereira de Melo, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

Priscila Oliveira da Silva, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

Saruanna Millena dos Santos Clemente, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil

Renata Pimentel Bandeira de Melo, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

José Givanildo da Silva, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

José Wilton Pinheiro Júnior, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

Belchiolina Beatriz Fonseca, Federal University of Uberlandia

Federal University of Uberlândia (UFU), Av. João Naves de Ávila, 2121 - Santa Mônica, Uberlândia - MG, 38408-100, Brazil.

Marcelo Mendonça, Federal University of Agreste of Pernambuco

Laboratorial Research Support Center of the Academic Unit of Garanhuns - (CENLAG), Federal University of Agreste de Pernambuco (UFAPE), Avenida Bom Pastor, no., Bairro Boa Vista, Garanhuns, PE 55292-270, Brazil.

Mércia Rodrigues Barros, Rural Federal University of Pernambuco

Department of  Veterinary Medicine, Federal Rural University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, no number, Dois Irmãos, Recife, PE 52171-900, Brazil.

References

Alves, J. M. C. S. & Oliveira, T. C. R. M. (2013). Presença de Campylobacter spp. em cortes refrigerados de frango. Semina: Ciências Agrárias, Londrina, v. 34(6), pp. 2829-2836. DOI: 10.5433/1679-0359.2013v34n6p2829.

Aquino, M. H. C., Pacheco, A. P. G., Ferreira, M. C. S. & Tibana. A. (2002). Frequency of isolation and identification of thermophilic Campylobacters from animals in Brazil. The Veterinary Journal, v.164(2), pp.159-165. DOI:10.1053/tvjl.2001.0698.

Azeredo, L. I.; Luchese, R. H. & Lauria-Filgueira, A. L. (2010). Campylobacter spp em carne de ave crua: avaliação da etapa de resfriamento. Revista Instituto Adolfo Lutz., v. 69(4), pp.518-524.

Bhunia, A. K. (2008). Foodborne microbial pathogens: mechanisms and pathogenesis. Purdue University, pp. 217-225. DOI: 10.1007 / 978-0-387-74537-4.

Boufleur, R. (2009). Campylobacter Jejuni em frango de corte, carne e vísceras de frango no Rio Grande Do Sul e efeito do congelamento sobre a contaminação nos cortes. Dissertação (Mestrado Em Medicina Veterinária) – Universidade Federal de Santa Maria - UFSM. Santa Maria.

Butzler, J.P. (2004). Campylobacter, From obscurity to celebrity. Clinical Microbiology And Infection, v.10(10), pp.868-876. DOI: 10.1111/j.1469-0691.2004.00983.x.

CDC - Centers For Disease Control And Prevention. (2012). Campylobacter Investigation Guideline. Kansas Disease Investigation Guidelines. Atlanta: Georgia.

CDC - Centers for Disease Control and Prevention. (2018). Campylobacter Investigation Guideline. Kansas Disease Investigation Guidelines. Version 05/2018. Atlanta: Georgia.

Cortez, A. L. L. (2003). Indicadores de qualidade higiênico-sanitária em linguiça frescal comercializada no município de Jaboticabal-SP. Dissertação De Mestrado, Universidade Estadual Paulista, Faculdade De Ciências Agrárias E Veterinárias, Jaboticabal.

Cortez A. L. L., Carvalho A. C. B. F., Scarcelli, L., Miashiro, S., Vidalmartins A. M. C. & Burger K. P. (2006). Survey of a chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli. Rev Inst Med Trop S Paulo. 48(6), pp. 307-310. DOI: 10.1590 / s0036-46652006000600001.

Costa, C. A. R. (2010). Avaliação da exposição do consumidor à Listeria monocytogenes, Salmonella spp., Campylobacter spp. e Escherichia Coli Produtora de toxina de shiga em produtos cárneos refrigerados comercializados no município de São Paulo. Tese (Doutorado) - Faculdade De Ciências Farmacêuticas, Universidade De São Paulo, São Paulo.

Cróinín, T. Ó. & Steffen, B. (2012). Host epithelial cell invasion by Campylobacter jejuni: trigger or zipper mechanism?. Front. Cell. Infect. Microbiol. v. 2(25) pp. 1-13. DOI: 10.3389/fcimb.2012.00025.

EFSA and ECDC (European Food Safety Authority And European Centre For Disease Prevention And Control). (2018). The European Union Summary Report On Trends And Sources Of Zoonoses, Zoonotic Agents And FoodBorne Outbreaks In 2017 .URL <https://efsa.onlinelibrary.wiley.com/DOI/epdf/10.2903/j.efsa.2018.5500 >.

EFSA (European Food Safety Authority) (2019). Scientific report on the European Union one health 2018 zoonoses report. EFSA Journal. URL: <https://DOI.org/10. 2903/j.efsa.2019.5926>.

Eucker, T. P. & Konkel, M. E. (2012). The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating membrane ruffling. Cellular Microbiology v.14(2), pp. 226-238. DOI: 10.1111 / j.1462-5822.2011.01714.x.

FAO/WHO- Food and Agriculture Organization of The United Nations/World Health Organization. (2009). Salmonella and Campylobacter In Chicken Meat: Meeting. Microbiological Risk Assessment Series n. 19. Rome, Italy.

Feistel, J. C., Sola, M. C., Medeiros, N. X., Trassi, A. M. C., Mesquita, S. Q. P., Silva Junior, M. C. & Rezende, C. S. M. D. E. (2012). Identificação e biotipificação de cepas de Campylobacter spp. isoladas de produtos avícolas. Anais Do Congresso De Pesquisa, Ensino e Extensão - Conpeex (2012). Anais.

Fitzgerald, C., Helsel, L. O. & Nicholson, M. A. (2001). Evaluation of methods for subtyping Campylobacter jejuni during an outbreak involving a food handler. J. Clin. Microbiol., v.39(7), pp. 2386-2390. DOI: 10.1128%2FJCM.39.7.2386-2390.2001.

Forsythe, S. J. (2000). Na Microbiologia Do Alimento Seguro. Microrganismos De Intoxicação Alimentar. 1ª Ed. pp. 87-148. Porto Alegre: Abingdon: Editores Da Blackwell Science.

Franchin, P. R., Aidoo, K. E. & Batista, C. R. V. (2005). Sources of poultry meat contamination with thermophilic Campylobacter before slaughter. Brazilian Journal Microbiology, v.36(2), pp.157-162. DOI: 10.1590/S1517-83822005000200011.

Franco, B. D. G. M. & Landgraf, M. (2005). Microbiologia Dos Alimentos. Atheneu.: São Paulo.

Frazier, W. C.; Westhoff, D. C. (1993). Microbiologia De Los Alimentos, 4° Edição, Editora Acribia, Zaragoza.

Freitas, J. A. & Noronha, G.N. (2007). Ocorrência de Campylobacter Spp. em carne e miúdos de frango expostos ao consumo em Belém, Pará. Arquivo Brasileiro De Medicina Veterinária E Zootecnia, v.59(3), pp. 813-815. DOI: 10.1590/S0102-09352007000300038.

Gorman, R. & Adley, C. C. (2004). An evaluation of five preservation techniques and conventional freezing temperatures of -20ºc and -85ºc for long-term preservation of Campylobacter jejuni. Letters Applied Microbiology v. 38(4), pp.306-10.

Invitrogen. Real-Time Pcr: From Theory To Practice - Handbook. (2008). 72f.

Jasson, V., Sampers, I., Botteldoorn, N., López-Gálvez, F., Baert, L., Denayer, S., Rajkovic, A., Habib, I., De Zutter, L., Debevere, J. & Uyttendaele, M. (2009). Characterization Of Escherichia coli from raw poultry in Belgium and impact on the detection of Campylobacter jejuni using Bolton broth. International Journal Of Food Microbiology, v. 135(3) pp. 248–253. DOI: 10.1016/j.ijfoodmicro.2009.09.007.

Maziero, M. T. & Oliveira, T. C. R. M. (2010). Effect of refrigeration and frozen storage on the Campylobacter Jejuni recovery from naturally contaminated broiler carcasses. Brazilian Journal Of Microbiology, v. 41(2), pp. 501-505. DOI: 10.1590/S1517-83822010000200034.

Medeiros, V. M. (2011). Isolamento e identificação fenotípica e molecular das espécies termofílicas de Campylobacter a partir de frango resfriado. (Dissertação De Mestrado Profissional). Fundação Oswaldo Cruz. Instituto Nacional De Controle De Qualidade Em Saúde, Programa De Pós-Graduação Em Vigilância Sanitária, Rio De Janeiro-RJ.

Medeiros V. M., Bricio S. M. L., Filgueiras A. L. L. & Clementino M. B. M. (2012). utilização de caldo Bolton no enriquecimento seletivo em comparação ao plaqueamento direto na pesquisa de Campylobacter spp. em carcaças resfriadas de frango. Revista Do Instituto Adolfo Lutz.v.71(3), pp.456-461. DOI: 10.98552012000300004&lng=es&tlng=es.

Moura Filho L. G. M., Bezerra S. S., Barros G. C., Melo H. M. G., & Mendes E. S. (2010). Perfil microbiológico da carne de frangos abatidos artesanalmente e na indústria, comercializados na grande Recife-PE. Medicina Veterinária, v.4(1) pp.12-17.

Mulinari, E. L., Salvatori R. U. & Majolo, C. (2014). Enumeração de Campylobacter em carcaças, cortes e miúdos de frango produzidos no rio grande do sul. Caderno Pedagógico. v.11(1), pp. 91-98. DOI: 10.22410/issn.1983-0882.

Nachamkin I, Szymanski C. M. & Blaser M. J. (2008). Campylobacter, 3 Ed. Emerging Infectious Diseases. Asm, Washington.

Notermans, S. & Hoogenboom-Vedergaal, A. (1992). Existing and emerging foodborne diseases. International Journal Of Food Microbiology, v. 15(3), pp. 197-205. DOI: 10.1016 / 0168-1605 (92) 90049-9.

Rozynek, E., Dzierzanowska-Frangat, K., Jozwiak, P., Popowski, J., Korsak, D. & Dzierzanowska, D. (2005). Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. Journal Med. Microbiol., v.54(7), pp. 615-619. DOI: 10.1099 / jmm.0.45988-0.

Santos, E. L. S. (2016). Detecção E Identificação De Campylobacter Spp. Em Carcaças De Frango De Corte Produzidas No Estado De Minas Gerais. (Dissertação De Mestrado). Universidade Federal De Minas Gerais, Belo Horizonte-MG.

Stern, N. J., Line, J. E. & Chen, H. C. (2001). Campylobacter In: Downes, F. P.; Ito, K. Chapter 31. Compendium Of Methods For The Microbiological Examination Of Foods, pp. (301-310). Washington, Dc: Apha.

Vandamme, P. (2000). Taxonomy Of The Family Campylobacteraceae. Campylobacter, 2 Ed. pp. (3-44). Washington.

Wieczorek, K., Denis, E., Lynch, O. & Osek, J. (2013). Molecular characterization and antibiotic resistance profiling of Campylobacter isolated from cattle in Polish slaughterhouses. Food Microbiology. v.34(1), pp. 130- 136. DOI: 10.1016 / j.fm.2012.12.003.

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Published

18/08/2021

How to Cite

MELO, F. P. de; SILVA, P. O. da; CLEMENTE, S. M. dos S. .; MELO, R. P. B. de .; SILVA, J. G. da; PINHEIRO JÚNIOR, J. W.; FONSECA, B. B.; MENDONÇA, M.; BARROS, M. R. Detection of Campylobacter jejuni, Campylobacter coli, and virulence genes in poultry products marketed in Northeastern Brazil. Research, Society and Development, [S. l.], v. 10, n. 10, p. e542101019224, 2021. DOI: 10.33448/rsd-v10i10.19224. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/19224. Acesso em: 21 oct. 2021.

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Agrarian and Biological Sciences