Comparative study of the chemical composition, larvicidal, antimicrobial and cytotoxic activities of volatile oils from E. punicifolia leaves from Minas Gerais and Goiás

Authors

DOI:

https://doi.org/10.33448/rsd-v10i11.19354

Keywords:

Essential oil; Chemodiversity; Medicinal plant; Biological activities; Myrtaceae.

Abstract

Eugenia punicifolia (Kunth) D.C., Myrtaceae, known as “pedra-ume-caá”, is popularly used in the treatment of inflammation, infections, fever, flu, diabetes, and diarrhea. This study aimed to carry out a comparative study of the chemical composition of volatile oil from E. punicifolia leaves collected in Goiás and Minas Gerais, as well as to evaluate the larvicidal activity against Aedes aegypti L3 larvae, the antimicrobial activity against bacteria, pathogenic fungi, and environmental, and cytotoxic activity to Balb 3T3 cells (murine fibroblasts). Volatile oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by Gas Chromatography Coupled to Mass Spectrometry (CG/MS). A total of 60 compounds were identified, the main components found in the leaves of Goiás being Germacrene D, bicyclogermacrene and β-longipenene and in the leaves collected in Minas Gerais they were (Z)-caryophyllene, γ-cadinene, spathulenol, caryophyllene oxide, and α-cadinol. The larvicidal effect was moderate against Ae. aegypti, with LC50 of 85.53 µg / mL for samples from Goiás and LC50 of 91.52 µg / mL for samples from Minas Gerais. Both oils showed moderate bactericidal activity against K. rhiziphyla (ATCC 9341), M. luteus (ATCC 10240), and S. aureus (ATCC 29737). The oils from Goiás (IC50 706.7 µg / mL) and Minas Gerais (IC50 160.7 µg / mL) had a lower cytotoxic concentration than the toxic action for larvae and bacteria, evidencing a safety profile and an interesting therapeutic potential, mainly concerning to volatile oil from Goiás. Therefore, the volatile oils from E. punicifolia leaves collected in Goiás and Minas Gerais that presented moderate larvicidal activity for Ae. aegypti also presented a bactericide activity and less cytotoxicity against murine fibroblasts. This is the first study of the larvicidal, antimicrobial and cytotoxic activity of volatile oils from E. punicifolia leaves.

Author Biographies

Liliane de Sousa Silva, Universidade Federal de Goiás

Possui graduação em Ciências Biológicas pela Universidade Estadual de Goiás (2012). Especialização em Neuropedagogia Aplicada à Educação (FABEC Brasil) 2013, Mestre em Ciências Farmacêuticas pela Universidade Federal de Goiás (UFG) 2013-2015. Doutoranda em Ciências Farmacêuticas (UFG) 2016 - 2019. Funcionário público (2010-2018) da Prefeitura Municipal de Itapuranga-GO, atuando na Secretaria de Saúde - Agência de Transfusão. Foi professora titular da Universidade Estadual de Goiás (UEG) (2014-2018) no curso de Licenciatura Plena em Ciências Biológicas nas disciplinas de: fisiologia vegetal, biologia molecular, bioestatística, biofísica, anatomia e fisiologia humana e comparativa e didática. Fui professor efetivo da Escola Estadual Paulo Freire em Primavera do Leste - MT (julho de 2018 a abril de 2019) nas aulas e 8º e 9º anos.

Christiane França Martins, Universidade Federal de Goiás

Farmacêutica, Mestre em Biotecnologia (UFPA), doutoranda em Ciências Farmacêuticas na Universidade Federal de Goiás, Faculdade de Farmácia, Brazil.

Fernando Yano Abrão, Universidade Federal de Goiás

Farmacêutico, Mestre em Relação Parasito-hospedeiro, doutorando em Ciências Farmacêuticas e Professor na Universidade de Campinas (Unicaps).

Camila Aline Romano, Universidade Federal de Goiás

Biologa, Mestre em Relação Parasito-hospedeiro, doutoranda em Inovação Farmacêutica.

Soraia Ferreira Bezerra, Universidade Federal de Goiás

Bióloga, Mestre em Ciências Farmacêutica e Doutoranda em Inovação Farmacêutica

Leonardo Luiz Borges, Universidade Estadual de Goiás

Professor doutor na Universidade Estadual de Goiás e Universidade Católica de Goiás. 

Adelair Helena dos Santos , Universidade Federal de Goiás

Professora doutora na Universidade Federal de Goiás, Laboratório de biologia e fisiologia de insetos no Instituto de Patologia Tropical e Saúde Publica.

Luiz Carlos da Cunha, Universidade Federal de Goiás

Professor doutor na Universidade Federal de Goiás, Núcleo de Estudos e Pesquisas Toxico-Farmacológicas (NEPET)

Jerônimo R. Oliveira Neto , Universidade Federal de Goiás

Químico, Técnico da Universidade Federal de Goiás, Núcleo de Estudos e Pesquisas Toxico-Farmacológicas (NEPET)

Tatiana de Sousa Fiuza , Universidade Federal de Goiás

Professora, Doutora da Universidade Federal de Goiás, Instituto de Ciências Biológicas.

José Realino de Paula, Universidade Federal de Goiás

Professor, Doutor da Universidade Federal de Goiás, Faculdade de farmácia, Laboratório de Pesquisa de Produtos Naturais

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22/08/2021

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SILVA, L. de S. .; MARTINS, C. F. .; ABRÃO, F. Y. .; ROMANO, C. A. .; BEZERRA, S. F. .; BORGES, L. L.; SANTOS , A. H. dos .; CUNHA, L. C. da .; OLIVEIRA NETO , J. R. .; FIUZA , T. de S. .; PAULA, J. R. de . Comparative study of the chemical composition, larvicidal, antimicrobial and cytotoxic activities of volatile oils from E. punicifolia leaves from Minas Gerais and Goiás. Research, Society and Development, [S. l.], v. 10, n. 11, p. e34101119354, 2021. DOI: 10.33448/rsd-v10i11.19354. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/19354. Acesso em: 22 nov. 2024.

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Health Sciences