Gold nanoparticles associated with temozolomide for glioblastoma Multiforme Treatment

Authors

DOI:

https://doi.org/10.33448/rsd-v10i11.19406

Keywords:

Glioblastoma; Nanopartículas de ouro; Temozolomida; Radioterapia.

Abstract

Malignant neoplasms represents a group of diseases that features, as a characteristic, the genetic differentiation of the original tissue, leading to the disordered growth of cells, invading normal tissues and organs. Among the most aggressive tumors, Glioblastoma Multiforme has a mortality rate around 95% and survival’s average of 15 months, even though all treatment available. Temozolomide (TMZ) is the chemotherapeutic drug so far tested and approved with the highest response in this tumor sub-type and must be associated to other treatments to achieve better results. Thus, the purpose of this work was to evaluate the performance of this therapeutic modality with gold nanoparticles (AuNPs) and also combined with radiotherapy. TMZ hydrolysis was characterized at different pH and the chemical changes on molecular structure was determined via Fourier Transform Infrared Spectroscopy (FT-IR). The treatment performance was verified in vitro test using TMZ, TMZ plus AuNPs and associated with radiotherapy. The TMZ concentrations were varied from 0 (control group) to 1000µM, combined with AuNPs from 0 (control group) to10¹⁰ nanoparticles per well. The results showed the drug is stable at pH values between 2 to 4, but for pH values close to the physiological or basic medium, degradation is accentuated reaching a rate of 16 %/hour. The changes on molecular structure of TMZ can be observed through the FT-IR spectra, where the release of oxygen in the structure has influence on C=O group. The results of in vitro experiments showed that the highest poor results in the absence of ionizing irradiation. However, for experiments with TMZ and nanoparticles associated to radiotherapy, the performance of the treatment increased. In summary, the AuNPs showed important results under irradiation, revealing the same level of cytotoxicity for the highest TMZ concentration without irradiation. Also, the synergic effect between AuNPs and TMZ was observed under irradiation condition.

References

Belter, A.; Barciszewski, J.; Barciszewska, A.-M. Revealing the epigenetic effect of temozolomide on glioblastoma cell lines in therapeutic conditions. PLoS ONE 2020, 15, e0229534

Bhat S.A, Ahmad S . Quantum chemical calculations and analysis of FTIR, FT–Raman and UV–Vis spectra of temozolomide molecule J. Mol. Struct., 1099 (2015), pp. 453-462.

Chelliah, S.S.; Paul, E.A.L.; Kamarudin, M.N.A.; Parhar, I. Challenges and Perspectives of Standard Therapy and Drug Development in High-Grade Gliomas. Molecules 2021, 26, 1169. https://doi.org/10.3390/molecules26041169

Dib, r. V.; gomes, a. M. T. .; ramos, r. De s. .; frança, l. C. M. .; marques, s. C. . Cancer and its social representations for cancer patients. Research, Society and Development, [S. l.], v. 9, n. 9, p. e187997134, 2020. DOI: 10.33448/rsd-v9i9.7134.

Friedman HS, Kerby T, Calvert H. Temozolomide and treatment of malignant glioma. Clin Cancer Res. 2000;6(7):2585–2597.

Hainfeld JF, Dilmanian FA, Slatkin DN, Smilowitz HM. Radiotherapy enhancement with gold nanoparticles. J Pharm Pharmacol. 2008 Aug;60(8):977-85. doi: 10.1211/jpp.60.8.0005. PMID: 18644191.

Melo, r. L. F.; souza, i. C. Da c.; carvalho, a. J. R.; bezerra, e. M.; costa, r. F. Da. Nanoparticles as biological tools: an exploratory review. Research, Society and Development, [S. l.], v. 9, n. 7, p. e363974155, 2020. DOI: 10.33448/rsd-v9i7.4155.

Mesbahi A. A review on gold nanoparticles radiosensitization effect in radiation therapy of cancer. Rep Pract Oncol Radiother. 2010;15(6):176-180. Published 2010 Oct 8. doi:10.1016/j.rpor.2010.09.001

Newlands ES, Stevens MF, Wedge SR, et al. Temozolomide: a review of its discovery, chemical properties, pre-clinical development and clinical trials. Cancer Treat Rev 1997;23:35-61. 10.1016/S0305-7372(97)90019-0

Orza A, Soriţău O, Tomuleasa C, et al. Reversing chemoresistance of malignant glioma stem cells using gold nanoparticles. Int J Nanomedicine. 2013;8:689‐702. doi:10.2147/IJN.S37481

Ostrom QT, Gittleman H, Fulop J, et al. CBTRUS Statistical Report: Primary Brain and Central Nervous System Tumors Diagnosed in the United States in 2008-2012. Neuro Oncol 2015;17 Suppl 4:iv1-iv62. 10.1093/neuonc/nov189

Ostrom QT, Gittleman H, Stetson L, Virk S, Barnholtz-Sloan JS. Epidemiology of Intracranial Gliomas. Prog Neurol Surg. 2018;30:1–11. doi:10.1159/000464374.

Pérez-Herrero E, Fernández-Medarde A. Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy. Eur J Pharm Biopharm. 2015;93:52–79. doi:10.1016/j.ejpb.2015.03.018.

Pouchieu C, Gruber A, Berteaud E, et al. Increasing incidence of central nervous system (CNS) tumors (2000-2012): findings from a population based registry in Gironde (France). BMC Cancer. 2018;18(1):653. Published 2018 Jun 14. doi:10.1186/s12885-018-4545-9.

Sandes , v. Dos a. .; dantas, r. L. .; porto, r. L. S. .; reis , f. F. P. .; sousa , d. S. De .; lima, s. O. .; marcena, s. L. S. .; reis, f. P. The occurrence of tumors of the central nervous system in the state of Sergipe from 2008 to 2017. Research, Society and Development, [S. l.], v. 9, n. 11, p. e3439119673, 2020. DOI: 10.33448/rsd-v9i11.9673.

Stupp R, Hegi ME, Mason WP, et al. Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study: 5-year analysis of the EORTC-NCIC trial. Lancet Oncol. 2009;10(5):459–466. doi:10.1016/S1470-2045(09)70025-7.

Tapan Behl, Aditi Sharma, Lalit Sharma, Aayush Sehgal, Sukhbir Singh, Neelam Sharma, Gokhan Zengin, Simona Bungau, Mirela Marioara Toma, Daniela Gitea, Elena Emilia Babes, Claudia Teodora Judea Pusta, Adrian Gheorghe Bumbu. Current Perspective on the Natural Compounds and Drug Delivery Techniques in Glioblastoma Multiforme. Cancers 2021, 13 (11) , 2765. https://doi.org/10.3390/cancers13112765

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65(2):87–108. doi:10.3322/caac.21262.

Vieira L, Castilho ML, Ferreira I, Ferreira-Strixino J, Hewitt K.C., Raniero L. Synthesis and characterization of gold nanostructured Chorin e6 forPhotodynamic Therapy, Photodiagnosis and Photodynamic Therapy 18 (2017) 6–11.

Wen PY, Reardon DA, et al. Neuro-oncology in 2015: Progress in glioma diagnosis, classification and treatment. Nat Rev Neurol 2016;12:69-70. 10.1038/nrneurol.2015.242

Downloads

Published

25/08/2021

How to Cite

GIALLUCA, V. D. .; LIMA, V. G. P. de .; CAIXETA, A.; CASTILHO, M. L. .; RANIERO, L. J. . Gold nanoparticles associated with temozolomide for glioblastoma Multiforme Treatment. Research, Society and Development, [S. l.], v. 10, n. 11, p. e146101119406, 2021. DOI: 10.33448/rsd-v10i11.19406. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/19406. Acesso em: 8 nov. 2024.

Issue

Section

Health Sciences