Phenolic Compunds and antioxidant capacity of the Clitoria ternatea L. flower extracts
DOI:
https://doi.org/10.33448/rsd-v10i11.19450Keywords:
Bioactive compound; DPPH; FRAP; Buterfly; Butterfly beans.Abstract
Clitoria ternatea L. (CT) is a plant native from Tropical Asia, popularly identified in Brazil as butterfly bean. Its extracts have a wide range of pharmacological activities, in addition, CT flowers have a significant amount of phenolic compounds and anthocyanins. The general goal of this study was to evaluate the effect of ethanol concentration on the extraction of phenolic compounds from CT flower and also evaluate the antioxidant capacity by the method based on the elimination of the free radical 1,1-diphenyl-2-picrylhydrazy (DPPH) and iron reduction (FRAP). The results presented by the research demonstrated that water at room temperature proved to be the best solvent for extracting phenolic compounds from CT flowers. Among the evaluated methods of antioxidant activity, only FRAP showed a strong correlation with the concentration of total phenolic compounds. The results of this study are extremely relevant due to the capacity of bioactive extraction that could be employed in the food industry.
References
Ali, S. S., Kasoju, N., Luthra, A., Singh, A., Sharanabasava, H., & Sahu, A. (2008). Indian medicinal herbs as sources of antioxidants. Food Research International, 41, 1-15.
Avan, A. N., Demirci Çekiç, S., Uzunboy, S., & Apak, R. (2016). Spectrophotometric determination of phenolic antioxidants in the presence of thiols and proteins. International Journal of Molecular Sciences, 17(8), 1325.
Banerjee, S. K., & Bonde, C. G. (2011) Total phenolic content and antioxidant activity of extracts of Bridelia Retusa Spreng Bark: Impact of dielectric constant and geographical location. Journal of Medicinal Plants Research, 5(5), 817-822.
Baskaran, A., Mudalib, S. K. A., & Izirwan, I. (2019). Optimization of aqueous extraction of blue dye from butterfly pea flower. Journal of Physics: Conference Series, 1358, 12001.
Berton-Carabin, C. C., Ropers, M. H., & Genot, C. (2014). Lipid Oxidation in oil-in-water emulsions: Involvement of the interfacial layer. Comprehensive Reviews in Food Science and food Safety, 13(5), 945-977.
Boroski, M., Visentainer, J. V., Cottica, S. M., & Morais, D.R. (2015). Antioxidantes: princípios e métodos analíticos, 1, 141.
Castañeda-Ovando, A., Pacheco-Hernández, M., Páez-Hernández, M., Rodríguez, J. A., & Galán-Vidal, C. A. (2009). Chemical studies of anthocyanins: A review. Food Chemistry, 113, 859-871.
Chayaratanasin, P., Barbieri, M. A., Suanpairintr, N., & Adisakwattana, S. (2015). Inhibitory effect of Clitoria ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro. BMC Complementary and Alternative Medicine, 15(27), 1-9.
Craft, B. D., Kerrihard, A. L., Amarowicz, R., & Pegg, R. B. (2012). Phenol-based antioxidants and the in vitro methods used for their assessment. Comprehensive Reviews in Food Science and Food Safety, 11, 148-173.
Ilayaraja, N., Likhith, K. R., Babu, G.R.S., & Khanum, F. (2015). Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM). Food Chemistry, 173, 348-354.
Kormin, F., Ahmed, I., Yunus, R. M., & Yusof, Z. A. M. (2010). Thepotential of modified microwave extraction system (MMES) to extract bioactive components from ferns. International Journal of Engineering and Technology, 10, 7–21.
Khoo, H. E., Azlan, A., Tang, S. T., & Lim, S. M. (2017). Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food & Nutrition Research, 61(1), 1361779.
Makasana, J., Dholakiya, B. Z., Gajbhiye, N. A., & Raju, S. (2017). Extractive determination of bioactive flavonoids from butterfly pea (Clitoria ternatea Linn.). Research on Chemical Intermediates, 43, 783-799.
Mehmood, A., Ishaq, M., Zhao, L., Yaqoob, S., Safdar, B., Nadeem, M., Munir, M., & Wang, C. (2019). Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). Ultrasonics sonochemistry, 51, 12-19.
Morris, J. (2009). Characterization of butterfly pea (Clitoria ternatea L.) accessions for morphology, phenology, reproduction and potential nutraceutical, pharmaceutical trait utilization. Genetic Resources and Crop Evolution, 56, 421-427.
Mustafa, A., & Turner, C. (2011). Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. Analytica Chimica Acta, 703, 8-18.
Nguyen, V. T., Bowyer, M. C., Vuong, Q. V., Altena, I. A. V., & Scarlett, C. J. (2016). Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) root as affected by various solvents extraction methods. Industrial Crops and Products, 67, 192-200.
Oliveira, H. A. B. (2019). Habits and food crops on the consumption of nonconventional vegetables by family farmers. Revista Agrária Acadêmica, 2(3), 17-32.
Oliveira, H.A.B., Anunciação, P. C., Silva, B. P., Souza, A. M. N., Pinheiro, S. S., Lucia, C. M. D., Cardoso, L. M., Castrol, L. C. V., & Podsędek, A. (2007). Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Science and Technology, 40 (1), 111.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da Pesquisa Científica. Santa Maria: UAB/NTE/UFSM.
Rufino, M. S. M. (2006). Metodologia científica: Determinação da atividade antioxidante total em frutas pelo método de redução do ferro (FRAP). Embrapa, Comunicado técnico 125.
Rufino, M. S. M. (2007). Metodologia científica: Determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Embrapa, Comunicado técnico 127.
Shen, Y., Du, L., & Zeng, H. (2016). Butterfly pea (Clitoria ternatea) seed and petal extracts decreased Hep-2 carcinoma cell viability. International Journal of Food Science and Technology, 51, 1860–1868.
Sheng, Z. L., Wan, P. F., Dong, C. L., & Li, Y. H. (2013). Optimization of yotal flavonoids content extracted from Flos populi using Response Surface Methodology. Industrial Crops and Products, 43, 778-786.
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics whif phophomolybdic-phospotungstic acid reagentes. American Journal of Enology and Viticulture, 16, 144-158.
Smeriglio, A., Barreca, D., Bellocco, E., & Trombetta, D. (2016). Chemistry, pharmacology and health benefits of anthocyanins. Phytotherapy Research: PTR, 30(8), 1265-1286.
Taylor, R. (1990). Interpretation of the correlation coefficient: a basic review. Journal of. Diagnostic Medical Sonography, 6, 35–39.
Zingare, M. L., Zingare, P.L., Dubey, A. K., & Ansari, M. A. (2013). Clitoria ternatea (APARAJITA): A review of the antioxidant, antidiabetic and hepatoprotective potential. International Journal of Pharmacy and Biological Science, 3(1), 201–210.
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