Analysis of aflatoxins and ergot alkaloids in feeds for adult equine
DOI:
https://doi.org/10.33448/rsd-v10i11.19697Keywords:
Fungi; Mycotoxins; Ergometrine; Griseofulvin; Festuclavin.Abstract
Mycotoxins are products resulting from fungi metabolism normally present in the environment and which can develop in food. The aim of this study was the fungi quantification, aflatoxin detection and investigation of ergot alkaloids occurrence in pelleted feed for adult equines during storage. The feed samples were collected from three rural properties with equideoculture activity in the city of Teresina, Piauí, Brazil. The results showed that was no significant difference (p<0.05) for the counting of colony-forming units (CFU/g) of filamentous fungi and yeasts in the samples. Aflatoxins B1, B2, G1 and G2 were found in acceptable amounts and the other fungal metabolites: ergometrine, griseofulvin, festuclavin, ergine and lysergol. Concluding, the results of this study demonstrate that amount of filamentous fungi and the water activity present in the original package remain constant after six days of storage. Four types of aflatoxins were found: AFB1, AFB2, AFG1, AFG2 and the Ergot alkaloids: ergine, ergometrine, festuclavine, griseofulvin and lisergol in amounts within acceptable limits. These groups of toxic compounds produced by fungi can be present in equine feed and may lead to a risk to their health.
References
Caldas, E. D., Silva, S. C., & Oliveira, J. N (2002), Aflatoxins and ochratoxin A in food and human health risks. Rev Saude Publ, 319-323.
Cardoso Filho, F. C., Calvet, R. M., Rosa, C. A. R., Pereira, M. M. G., Costa, A. P. R., & Muratori, M. C. S. (2013), Monitoring of toxigenic fungi and aflatoxins in feeds used in fish farming, Ciência animal Brasileira 305-311. https://doi.org/10.5216/cab.v14i3.15414
Dalcero, A., Magnoli, C., Chiacchiera, S., Palacios, G., & Reynoso, M. (1997), Mycroflora and incidence of afaltoxin B1, zearalenone and deoxynivalenol in poultry feeds in Argentina. Mycopathologia, 179-184. https://doi.org/10.1023/A:1006890717758
Dalcero, A., Magnoli, C., Luna, M., Ancasi, G., Reynoso, M., Chiacchiera, S., Miazzo, R., & Palacio G. (1998), Mycoflora and naturally ocurring mycotoxins in poultry feds in Argentina. Mycopathologia, 37-43. https://doi.org/10.1023/A:1006868002985
Decagon Devices, (2016). Inc. 2365 NE Hopkins Court, Pullman, USA, WA 99163 10341.
Dias, T. F. V., Arcanjo, L. L., Costa, G. L; Souza, C. S., Lima, C. A. R. (2020), Controle de pragas e tratamento de grãos armazenados para uso em rações para animais. Research, Society and Development, [S. l.], v. 9, n. 9, p. e739996964. https://doi.org/10.33448/rsd-v9i9.6964
EU, European Union. (2002) Directive 2002/32/EC of the European Parliament and of the Council of 7 May 2002 on undesirable substances in animal feed, 10–21.
FAO/WHO. Food and Agricultural Organization and World Health Organization, (2011), Evaluation of certain food additives and contaminants: seventy-fourth report of the Joint FAO/WHO Expert Committee on Food Additives. WHO technical report series.n. 966.
Fayrer-Hosken, R. A., Hill, N. S., Heusner, G. L., Traylor-Wiggins, W., & Turner, K. (2013), The effects of ergot alkaloids on the breeding stallion reproductive system. Equine Veterinary Journal, 45, 44–47. https://doi.org/10.1111/evj.12164
FDA-Food and Drug Administration (2001), Federal Register. Guidance for Industry: Fumonisin levels in human foods and animal feeds. U.S. Center for Food Safety and Applied Nutrition, Center for Veterinary Medicine, November 9.
Ferreira, R. A. (2012), Swine: Practical Handbook of Creation.Viçosa, MG: Aprenda Fácil, 443 p.
Gabbi, A. M., Cypriano, L., & Piccinin, I. (2011). Aspectos microbiológicos e físico-químicos de três rações comerciais sob diferentes condições de armazenamento. Revista Brasileira de Saúde e Produção Animal, 12, 784-793.
Good Manufacturing Practices (GMP) (2016). GMP + BA1 Specific Feed, specific feed safety limits. 84 p. URL:( https://www.gmpplus.org/pagina/9/standards.aspx) access 19 May 2020.
Haschek, W. M., Voss, K. A., & Beasley, V. R. (2002). Selected Mycotoxins Affecting Animal and Human Health. Academic Press, 683-690.
https://doi.org/10.1016/B978-012330215-1/50026-0
Hillmann, B., Soriano, V. S., Petrolli, T. G. & Maccari, M. (2015). Microbiological analysis of diets for dogs marketed in bulk and in closed packaging, Enciclopédia biosfera, 134.
Liesener, K., Curtui, V., Dietrich, R., Märtlbauer, E., & Usleber, E. (2010). Mycotoxins in horse feed. Mycotox Res, 23–30. http://doi.org/10.1007/s12550-009-0037-8
Mallmann, C. A. (2014), Mycotoxins: how to manage risk and minimize the problem. Rev AviSite, 66.
Mallmann, C. A. & Dikin, P. (2011). Mycotoxins and Mycotoxicoses in Swine. Special nutrients: the Mycotoxins specialist.
Melo Filho, A. B. & Vasconcelos, M. A. S. (2011), Food Chemistry. UFRPE.78p.
Mori, C., Nascimento Junior, A., & Miranda, M. Z., (2013). Economic and conjunctural aspects of rye culture in the world and in Brazil. Deep step. Embrapa-CNPT.
Pinto, G. A. S., Brito, E. S., Silva, F. L. H., Santos, S. F. M., & Macedo, G. R. (2006). Fermentation in solid state an alternative for the use and valorization of agroindustrial residues. Rev. Quím. Ind., 17-20.
Pitt, J. I., & Hocking, A. D. (2009). Fungi and spoliage (2nd ed.), Blackie academic and professional.
Ribeiro, L. B., Arruda, A. M. V., Pereia, E. S., Tonello, C. L., Barreto, J.C. (2009) Evaluation of nutrient and water intake by horses fed diets containing different agroindustrial by - products. Uruguayan. 120- 133.
Santos, E. L., Cavalcanti, M. C. A., Livia, J. E., & Meneses, D. R. (2012). Nutritional and nutritional management of horses. Revisão. Revista eletrônica Nutritime, 1911-1943.
Santos, Y. F. M., Guimarães, C. M. M., Nunes, E. M. C. G., Calvet, R. M., Ferreira L. C. R. P., & Muratori, M. C. S. (2011), Toxigenic fungi in feeds for broiler chicken produced in Teresina, Piauí. Higiene Alimentar, 533-534.
Silva, F. C., Chalfoun, S. M., Batista, L. R., Santos, C., & Lima, N. (2015). Polyphase taxonomy for identification of Aspergillus flavi section: a review. Revista IFES Ciência, 18-40. https://doi.org/10.36524/ric.v1i1.235
Silva, N. (2010). Manual of methods of microbiological analysis of food and water. (4th ed.), Livraria Varela. 624 p.
Soares, L. M. V., & Rodriguez-Amaya, D. B. (1989). Survey of aflatoxins, ochratoxin A, zearalenone, and sterigmatocystin in some Brazilian foods by using multi-toxin thin-layer chromatographic method. Journal of the Association of Official Analytical Chemists, 72:22-6.
Teixeira, A. S., Freitas-Silva, O., Godóy, R. L. O., Vargas, E. A., & Martins, A. (2008). Analysis and quantification of aflatoxins by high performance liquid chromatography (HPLC) in Brazil nut samples. Revista Ciência da Vida, p.22-24.
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Copyright (c) 2021 Rafael Gomes Abreu Bacelar; Márcio dos Santos Rocha; João Farias Sousa Junior; Juliana Alexandre Ianiceli; Camila Maria Coutinho Moura; Aline Maria Dourado Rodrigues; Luciana Muratori Costa; Maria Christina Sanches Muratori
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