Comparative analysis of bacteriuria results on routine urinalysis and urine culture: a retrospective study
DOI:
https://doi.org/10.33448/rsd-v10i12.19711Keywords:
Bacteriuria; Canine; canine; cystitis; Cystitis; urinary tract infeccion; Urinary tract infection.Abstract
Bacterial urinary tract infections (UTI) are among the most frequent infectious diseases of small animals. Although antimicrobial therapy is recommended for treating bacterial UTIs, the current consensus is that treatment may not be necessary for asymptomatic animals. The aim of this study was to evaluate the diagnostic ability of urinalysis to detect bacteriuria and to compare it with urine culture (gold standard method) to assess the risk of false-positive results. A retrospective study was conducted from January 2016 to July 2019 and urine samples of 119 dogs were analyzed. Diagnostic validation was performed for urinalysis, based on the morphological classification and intensity of bacteriuria. Agreement between the results was assessed using the kappa (k) index. When the presence of cocci was used as a diagnostic criterion to suggest bacteriuria, it was observed that the agreement (k = -0.006) was lower than that expected by chance. However, a poor agreement (k = 0.22) was also found for bacilli during urinalysis (k = 0.23). A significant degree of agreement was observed in cases with high intensity of bacteriuria. Therefore, urine culture must be performed for conclusive evidence of bacteriuria to avoid false-positive results during urinalysis.
References
Adamama-Moraitou, K. K., Pardali, D., Prassinos, N. N., Menexes, G., Patsikas, M. N., & Rallis, T. S. (2017). Evaluation of dogs with macroscopic haematuria: a retrospective study of 162 cases (2003-2010). New Zealand Veterinary Journal, 65(4), 204–208.
Andrade, S. S., Sader, H. S., Jones, R. N., Pereira, A. S., Pignatari, A. C. C., & Gales, A. C. (2006). Increased resistance to first-line agents among bacterial pathogens isolated from urinary tract infections in Latin America: time for local guidelines? Memorias Do Instituto Oswaldo Cruz, 101(7), 741–748.
Barsanti, J. A. (n.d.). Greene: Infectious Diseases of the Dog and Cat · Chapter 90. Retrieved August 12, 2021, from https://www.greeneinfectiousdiseases.com/0090.php
Bartges, J. W. (2004). Diagnosis of urinary tract infections. The Veterinary Clinics of North America. Small Animal Practice, 34(4), 923–933, vi.
Brložnik, M., Šterk, K., & Zdovc, I. (2016). Prevalence and resistance patterns of canine uropathogens in regard to concurrent diseases. Berliner Und Munchener Tierarztliche Wochenschrift, 129(7-8), 340–350.
Carvalho, V. M., Spinola, T., Tavolari, F., Irino, K., Oliveira, R. M., & Ramos, M. C. C. (2014). Infecções do trato urinário (ITU) de cães e gatos: etiologia e resistência aos antimicrobianos. Pesquisa veterinaria brasileira: revista do Colegio Brasileiro de Patologia Animal = Brazilian journal of veterinary research, 34(1), 62–70.
Faria, P. F. de, Araújo, D. F. de, & Soto-Blanco, B. (2018). Glicemia em cães obesos e senis. Acta Scientiae Veterinariae, 33(1), 47.
Hall, J. L., Holmes, M. A., & Baines, S. J. (2013). Prevalence and antimicrobial resistance of canine urinary tract pathogens. The Veterinary Record, 173(22), 549.
Ishii, J. B., Freitas, J. C., & Arias, M. V. B. (2011). Resistência de bactérias isoladas de cães e gatos no Hospital Veterinário da Universidade Estadual de Londrina (2008-2009). Pesquisa veterinaria brasileira: revista do Colegio Brasileiro de Patologia Animal = Brazilian journal of veterinary research, 31(6), 533–537.
Johnson, J. R., Kaster, N., Kuskowski, M. A., & Ling, G. V. (2003). Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection. Journal of Clinical Microbiology, 41(1), 337–345.
Kim, Y., Jin, D. C., Lee, E. J., Lee, D. H., Chung, H. H., Kim, M., Lim, J., Lee, A., Lee, K. Y., Kang, C. S., Han, K., & Pai, S. H. (2002). Quantitative analysis of urine sediment using newly designed centrifuge tubes. Annals of Clinical Laboratory Science, 32(1), 55–60.
Knottnerus, J. A., van Weel, C., & Muris, J. W. M. (2002). Evaluation of diagnostic procedures. BMJ , 324(7335), 477–480.
Landis, J. R., & Koch, G. G. (1977). An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers. Biometrics, 33(2), 363–374.
McGhie, J. A., Stayt, J., & Hosgood, G. L. (2014). Prevalence of bacteriuria in dogs without clinical signs of urinary tract infection presenting for elective surgical procedures. Australian Veterinary Journal, 92(1-2), 33–37.
Oliver, J. D. (2005). The viable but nonculturable state in bacteria. Journal of Microbiology , 43 Spec No, 93–100.
O’Neil, E., Horney, B., Burton, S., Lewis, P. J., MacKenzie, A., & Stryhn, H. (2013). Comparison of wet-mount, Wright-Giemsa and Gram-stained urine sediment for predicting bacteriuria in dogs and cats. The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 54(11), 1061–1066.
Penna, B., Varges, R., Martins, R., Martins, G., & Lilenbaum, W. (2010). In vitro antimicrobial resistance of staphylococci isolated from canine urinary tract infection. The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 51(7), 738–742.
Peterson, A. L., Torres, S. M. F., Rendahl, A., & Koch, S. N. (2012). Frequency of urinary tract infection in dogs with inflammatory skin disorders treated with ciclosporin alone or in combination with glucocorticoid therapy: a retrospective study. Veterinary Dermatology, 23(3), 201–e43.
Pitout, J. D. D. (2012). Extraintestinal Pathogenic Escherichia coli: A Combination of Virulence with Antibiotic Resistance. Frontiers in Microbiology, 3, 9.
Procop, G. W., Church, D. L., Hall, G. S., & Janda, W. M. (2020). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Jones & Bartlett Learning.
Silva, J. M., & Lima, J. A. S. (2007). Quatro abordagens para o movimento browniano. Revista Brasileira de Ensino de Física, 29(1), 25–35.
Silva RS Paes A. (2012). Por dentro da estatística. Educ Contin Saúde Einstein., 10(4), 165–166.
Sørensen, T. M., Holmslykke, M., Nordlund, M., Siersma, V., & Jessen, L. R. (2019). Pre-test probability of urinary tract infection in dogs with clinical signs of lower urinary tract disease. Veterinary Journal , 247, 65–70.
Sørensen, T. M., Jensen, A. B., Damborg, P., Bjørnvad, C. R., Guardabassi, L., & Jessen, L. R. (2016). Evaluation of different sampling methods and criteria for diagnosing canine urinary tract infection by quantitative bacterial culture. Veterinary Journal , 216, 168–173.
Swenson, C. L., Boisvert, A. M., Kruger, J. M., & Gibbons-Burgener, S. N. (2004). Evaluation of modified Wright-staining of urine sediment as a method for accurate detection of bacteriuria in dogs. Journal of the American Veterinary Medical Association, 224(8), 1282–1289.
Thompson, M. F., Litster, A. L., Platell, J. L., & Trott, D. J. (2011). Canine bacterial urinary tract infections: new developments in old pathogens. Veterinary Journal , 190(1), 22–27.
Tivapasi, M. T., Hodges, J., Byrne, B. A., & Christopher, M. M. (2009). Diagnostic utility and cost-effectiveness of reflex bacterial culture for the detection of urinary tract infection in dogs with low urine specific gravity. Veterinary Clinical Pathology / American Society for Veterinary Clinical Pathology, 38(3), 337–342.
Van Nostrand, J. D., Junkins, A. D., & Bartholdi, R. K. (2000). Poor predictive ability of urinalysis and microscopic examination to detect urinary tract infection. American Journal of Clinical Pathology, 113(5), 709–713.
Way, L. I., Sullivan, L. A., Johnson, V., & Morley, P. S. (2013). Comparison of routine urinalysis and urine Gram stain for detection of bacteriuria in dogs. Journal of Veterinary Emergency and Critical Care , 23(1), 23–28.
Webb, J. (2016). Renal Cytology. In A. M. Barger & A. MacNeill (Eds.), Small Animal Cytologic Diagnosis (Illustrated edição, pp. 357–360). CRC Press.
Weese, J. S., Blondeau, J., Boothe, D., Guardabassi, L. G., Gumley, N., Papich, M., Jessen, L. R., Lappin, M., Rankin, S., Westropp, J. L., & Sykes, J. (2019). International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats. Veterinary Journal , 247, 8–25.
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Copyright (c) 2021 Ana Clara Moreira Pessôa Monteiro; Julio Cèsar Cambraia Veado; Matheus Matioli Mantovani; Raphael Rocha Wenceslau; Nathália das Graças Coelho Dorneles; Felipe dos Santos Muniz; Juliana Aires Favato; Adriane Pimenta da Costa Val
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