α-amylase production obtained from Aspergillus niger ATCC 1004 by solid state fermentation using Croton linearifolius residues as substrate
DOI:
https://doi.org/10.33448/rsd-v10i11.19891Keywords:
Bioprocesses; Enzyme kinetics; Optimization.Abstract
The objective of this work was to optimize the production and characterize α-amylase produced by Aspergillus niger through solid state fermentation, using leaf residues of C. linearifolius as substrate. For optimization, the incubation temperature, initial humidity and fermentation time were combined based on Doehlert experimental design. The highest productivity of the enzyme was 122.88 Ug-1, at 33oC, 70% humidity and 14 days of time. In the enzymatic characterization, the enzyme extract presented pH 5.0 and temperature 50oC, and α-amylase was thermostable up to 60oC, maintaining more than 90% of the activity. In evaluation of the effect of salt addition, sodium carbonate, calcium chloride, iron chloride, and cobalt chloride increased the enzymatic activity of α-amylase, while potassium and sodium from their chlorides served as enzyme inhibitors. The Km and Vmax values found were 0.04 mg/mL and 46.95 µmol/min/mL, respectively, indicating that the substrate has affinity for α-amylase. Therefore, the results demonstrate that the residues of C. linearifolius can be used as a substrate for A. niger in the production of enzymatic extracts, such as α-amylase.
References
Adejuwon, A. O., Tsygankova, V. A., & Alonge, O. (2018). Effect of cultivation conditions on activity of a-amylase from a tropical strain Aspergillus flavus Link. J. Microbiol. Biotech. Food Sci. 7 (6), 571-575.
Aisien, E. T., & Igbinosa, I. H. (2019). Production, purification, and characterization of a-amylase from Aspergillus niger, Aspergillus flavus and Penicillium expansum using cassava peels as substrate. Nig. J. Biotech. 36 (2), 114 -126.
Ali, I., Akbar, A., Anwar, M., Prasongsuk, S., Lotrakul, P., & Punnapayak, H. (2015). Purification and Characterization of a Polyextremophilic a-Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergent. BioMed Res. Int., 01-08.
Asrat, B., & Girma, A. (2018). Isolation, production and characterization of amylase enzyme using the isolate Aspergillus niger FAB-211. Int. J. Biotechnol. Mol. Biol. Res. 9(2), 7-14.
Cordeiro, I., Secco, R., Carneiro-Torres, D. S., Lima, L. R de, Caruzo, M. B. R., Berry, P., Riina, R., Silva, O. L. M., Silva, M. J da; & Sodre, R. C. (2015). Croton in Lista de Especies da Flora do Brasil. Jardim Botanico do Rio de Janeiro.
Dey, T. B., & Banerjee, R. (2015). Purification, biochemical characterization and application of a-amylase produced by Aspergillus oryzae IFO-30103. Biocatal. Agric. Biotechnol. 4 (1), 83-90.
El-Feky, R. M., Fattah, A. F. A. K. A., Gibriel, A. Y., & Farag, A. A. (2019). Optimization the parameter process of solid-state fermentation to produce the fungal a-amylase on agro-industrial by-products. AUJAS. Ain Shams Univ. Special Issue, 27(1), 441-456.
Freitas, S. L., Silva, T. M., Franco, M., Bonomo, P., & Freitas, J. S de. (2017). Fermentacao de farelo de mandioca, para obtencao de xilanase, a partir de um fungo endofitico. Enciclopedia Biosfera. Centro Cientifico Conhecer. 14(26), 995-1008.
Ghose, T. K. (1987). Measurement of cellulase activities. Pure & app. Chem. 59 (2), 257-268.
Gonzalez, G. V., Torres, E. F., Aguilar, C. N., Gomes, S. J. R., Godinez, G. D., & Augur, C. (2002). Advantages of fungal enzyme production in solid state over liquid fermentation systems. Biochem. Eng. J. 3643, 1-11.
Kanti, A., & Sudiana, I. M. (2018). Production of Phytase, Amylase and Cellulase by Aspergillus, Rhizophus and Neurospora on Mixed Rice Straw Powder and Soybean Curd Residue. IOP Conf. Ser. Earth Environ. Sci. 166.
Lineweaver, H., & Burk, D. (1934). The determination of enzyme dissociation constants. J. Am. Chem. Soc. 56, 658-666.
Mahmood, S., Shahid, M. G., Irfan, M., Nadeem, M., & Syed, Q. (2018). Partial Characterization of a-amylase Produced from Aspergillus niger using Potato Peel as Substrate. Punjab Univ. J. Zoo. 33(1), 22-27.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31(3), 426-428.
Mukherjee, R., Tanmay, P., Soren, JP., Halder, S. K., Mondal, K. C., Pati, B. R., & Das Mohapatra, P. K. (2019). Acidophilic a-Amylase Production from Aspergillus niger RBP7 Using Potato Peel as Substrate: A Waste to Value Added Approach. Waste Biomass. Valor. 10, 851-863.
Nelson, D. L., & Cox, M. M. (2014). Principios de Bioquimica de Lehninger. (6a ed.), Editora Artmed.
Okolo, B. N., Ezeogu, L. I., & Mba, C. N. (1995). Production of raw starch digestive amylase by Aspergillus niger graown on native starch sources. J. Sci. Food Agric. 69, 109-115.
Onofre, S. B., Abatti, D., Refosco, D., Tessara, A. A., Onofre, J. A. B., & Tessaro, A. B. (2016). Characterization of a-amilase produced by the endophytic straim of penicillium digitatym in solid state fermentation (SSF) and submerged fermentation. Afr. J. Biotechnol. 5 (28), 1511-1519.
Pandey, A. (2003). Solid state fermentation. Biochem. Eng. J. 13(2/3), 81-84.
Panesar, R., Kaur, S., & Panesar, P. S. (2015). Production of microbial pigments utilizing agro-industrial waste: a review. Curr. Opin. Food Sci. 1, 70-76.
Ravindran, R., Hassan, S. S., Williams, G. A., & Jaiswal, A. K. (2018). A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes. Bioengineering. 5 (4), 1-20.
Reilly, C. M., Kim, J., Lynn, J., Simmons, B. A., Gladden, J. M., Magnuson, J. K., & Baker, S. (2018). Forward genetics Screen coupled with whole-genome resequencing identifics novel gene targets for improving hetendogous enzyme production in Aspergillus niger. Appl. Microbiol. Biotechnol. 102(4), 1797-1807.
Reguly, J. C. (2000). Biotecnologia dos processos fermentativos: producao de enzimas e engenharia das fermentacoes. Pelotas: Editora Universitaria.
Sadh, P. K., Duhan, S., & Duhan, J. S. (2018). Agro-industrial wastes and their utilization using solid state fermentation: a review. Bioresour. Bioprocess. 5(1), 1-15.
Shah, I. J., Gami, P. N., Shukla, R. M., & Acharya, D. K. (2014). Optimization for a-amylase production by Aspergillus oryzae using submerged fermentation technology. Basic Res. J. Microbiol. 1(4), 01-10.
Shafique, S., & Shafique, S. (2017). Partial purification and characterization of a-amylase from hyperactive mutants of Aspergillus niger for economic enzyme production and starch hydrolysis. Int.J.Biol. Biotech., 14 (2), 159-167.
Santana, L. S. M. de, Goncalves, Z. S., & Franco, M. (2012). A producao de amilase a partir da fermentacao em estado solido do farelo de cacau. Enciclopedia Biosfera, Centro Cientifico Conhecer, 8 (14), 1981-1982.
Santos, I. R., Mendes, T. P. S., Miranda, A. C. A., Costa, D. N., Figueroa, G. M., Soares, V. D. M., Valasques Jr, G. L., & Cedro, P. E. P. (2020). Production and characterization of amylase obtained from Rhizopus microsporus var. oligosporus. Research, Society and Development, 9(7), 1-13.
Santos, J. C., Leal, I. R., Cortez, J. S. A., Fernandes, G. W. & Tabarelli, M. (2011). Caatinga: The scientific negligence experienced by a dry tropical forest. Trop. Conserv. Sci. 4 (3), 276-286.
Santos, P. S. dos, Solidade, L. S., Souza, J. G. B., Lima, G. S., Braga Jr, A. C. R., Assis, F. G. V de, & Leal, P. L. (2018). Fermentacao em estado solido em residuos agroindustriais para producao de enzimas: uma revisao sistematica. JCEC. 4 (2).
Shruthi, B. R., Achur, R. N. H., & Boramuthi, T. N. (2020). Optimized Solid State Fermentation Medium Enhances the Multienzymes Production from Penicillium citrinum and Aspergillus clavatus. Curr. Microbiol. 77, 2192 - 2206.
Silva, S. L. C., Gualberto, S. A., Carvalho, K. S., & Fries, D. D. (2014). Avaliacao da atividade larvicida de extratos obtidos do caule de Croton linearifolius Mull. Arg. (Euphorbiaceae) sobre larvas de Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae). Biotemas. 27(2), 79-85.
Silva, T. S. S., Freitas, J. S., Santos, E. S. L. dos, Cardoso, T. S., & Cerqueira-Silva, C. B. M. (2018). Caracterizacao e selecao de marcadores moleculares em Croton linearifolius Mull. Arg. como subsidio para estudos geneticos. Multi-Science Journal. 1 (10), 4-8.
Tung, Q. T., Miyata, N., & Iwahori, K. Growth of Aspergillus oryzae during treatment of cassava starch processing wastewater with higt contente of suspended solids. J. Biosci. Bioeng. 97(5), 329-335.
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Copyright (c) 2021 Juliana Antunes da Luz; Dhiéssica dos Santos Ribeiro; Jabson Menezes Teixeira; Thamilly Moreira Silva; Danilo Junqueira Leão; Marcelo Franco; Simone Andrade Gualberto; Janaína Silva de Freitas
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