Histochemistry study of the jejunum and the cecum in commercial laying hens submitted to the different forced molting programs





Poultry farming; Laying hens; Glycocalyx; Nutrition.


The objective of this study was to assess the alterations in the glycoproteins of the jejuno and the ceco of commercial poetry by histochemical techniques, and they will be subjected to different programs of forced molting. The study occurred in aviary of FCAV-Unesp, Jaboticabal campus. Thirty-two 58-week old Hisex Brown laying hens were used, housed in laying cages (two birds/cage) and randomly distributed in four forced molting programs with two production cycles, ending in 28 and 140 days, respectively. Four birds were allocated to each group, distributed into: Group 1 (G1), in which the California method was used as a control; group 2 (G2), in which a low-calcium diet was used; group 3 (G3), in which a diet with a high level of zinc was provided, and for group 4 (G4), a diet with a low level of sodium. The fragments of jejunum and cecum were submitted to the histochemistry techniques: PAS, PAS + amilase, AB 0,5 and AB 2,5 and were observed the glycoprotein, glycogen, sulfated and carboxyled polysacharides, respectively. The results suggest that the different treatments of forced molting in the experimental conditions did not interfere in the integrity of intestinal cells of these segments. It is concluded that the four treatments of forced molting did not interfere with the presence of intestinal cellular polysaccharides as well as with the integrity of the morphology of the intestinal cells and the glycocalyx.


ABPA (2020). Associação Brasileira de Proteína Animal. Relatório Anual da ABPA 2020. São Paulo: ABPA.

Alodan, M. A & Mashaly, M. M (1999). Effect of induced molting in laying hens on production and immune parameters. Poultry Science. 78 (2), 171-177.

Alroy, J., Goyal, V., Lukacs, N. W., Taylor, R. L., Strout, R. G., Ward, H. D & Pereira, M. E (1989). Glycoconjugates of the intestinal epithelium of the domestic fowl (Gallus domesticus): a lectin histochemistry study. The Histochemical Journal. 21 (4), 187-93.

Aygun, A (2013). Effects of force molting on eggshell colour, egg production and quality traits in laying hens. Revue de Médecine Vétérinaire. 164 (2), 46-51.

Bacha, W. J & Bacha, L. M (2003). Atlas colorido de histologia veterinária. 2nd ed. Ed. Roca: São Paulo. 456p. Portuguese.

Balaban, R. S., Bose, S., French, A. S., & Territo, P. R (2003). Role of calcium in metabolic signaling between cardiac sarcoplasmic reticulum and mitochondria in vitro. American Journal of Physiology-Cell Physiology. 284 (2), C285-93.

Bar, A., Razaphkovsky, V., Shinder, D & Vax, E (2003). Alternative procedures for molt induction: Practical aspects. Poultry Science. 82 (4), 543-550.

Bertechini, A. G & Geraldo, A (2005). Conceitos modernos em muda forçada de poedeiras comerciais. In: Simpósio Goiano de Avicultura, 7º, Simpósio Goiano de Suinocultura, 2º, Goiânia. Seminários Técnicos de Avicultura. Goiânia. p. 1-13.

Boonsoongnern, P., Saengprapaitip, K., Srisai, D., & Suprasert, D. A (2007). Glycoconjugates characterization in jejunal goblet cell of the chicken by means of lectin histochemistry. Kasetsart Veterinarians 17 (2), 73-79.

Carvalho, H. F., & Recco-Pimentel, S. M. A Célula. 4th ed. Editora Manole: São Paulo. 2019. 672 p. Portuguese.

Contreras, L., Drago, L., Zampese, E & Pozzan, T (2010). Mitochondria: The calcium connection. Biochimica et Biophysica Acta (BBA) – Bioenergetics. 1797 (6-7), 607-618.

Decuypere, E., & Verheyen, G (1986). Physiological basics of induced molting and tissue regeneration in fowls. World's Poultry Science Journal. 42 (1), 56-68.

El-Hack, M. E. A., Alagawany, M., Amer, S. A., Arif, M., Wahdan, K. M. M., & El-Kholy, M. S (2018). Effect of dietary supplementation of organic zinc on laying performance, egg quality and some biochemical parameters of laying hens. Journal of Animal Physiology and Animal Nutrition. 102 (2), e542–e549.

Feng, J., Ma, W. Q., Niu, H. H., Wu, X.M., Wang, Y & Feng, J. (2010). Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biological trace element research. 133 (2), 203–211.

Fletcher, O. J & Abdul-Aziz, T. A (2016). Alimentary System. In: ABDUL-AZIZ TA, FLETCHER OJ, BARNES HJ. Avian Histopathology. 1 ed. Ed. American Association of Avian Pathologists. p. 271- 354. English.

Griffiths, E. J & Rutter, G. A (2009). Mitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells. Biochim Biophys Acta. 1787 (11), 1324-33.

Idowu, O. M. O., Ajuwon, R. O., Oso, A. O & Akinloye, O. A (2011). Effects of Zinc Supplementation on Laying Performance, Serum Chemistry and Zn Residue in Tibia Bone, Liver, Excreta and Egg Shell of Laying Hens. nternational Journal of Poultry Science. 10 (3), 225-230.

Junqueira, L. C., Carneiro, J & Abrahamsohn, P (2017). Histologia básica: texto e atlas. 13. ed. Rio de Janeiro: Guanabara Koogan. 568p. Portuguese.

Kiela, P. R & Ghishan, F. K (2016). Physiology of Intestinal Absorption and Secretion. Best practice & research. Clinical gastroenterology. 30 (2),145-159.

Koch, J. M., Moritz, J. S., Lay, J .R., & Wilson, M. E (2007). Effect of melengestrol acetate as an alternative to induce molting in hens on the expression of yolk proteins and turnover of oviductal epithelium. Animal Reproduction Science. 102 (1-2), 14–23.

Laudadio, V., Passantino, L., Perillo, A., Lopresti, G., Passantino, A., Khan, R., U & Tufarelli, V (2012). Productive performance and histological features of intestinal mucosa of broiler chickens fed different dietary protein levels. Poultry Science. 91 (1), 265-70.

Li, L., Li, H., Zhou, W., Feng, J., & Zou, X (2019). Effects of zinc methionine supplementationon laying performance, zinc status, intestinalmorphology, and expressions of zinctransporters’ mRNA in laying hens. Journal of the Science of Food and Agriculture. 99 (14), 6582–658.

Li, W., Angel, R., Kim, S. W., Juménez-Moreno, E., Proszkowiec-Weglarz, M., & Plumstead, P. W (2018). Impacts of age and calcium on Phytase efficacy in broiler chickens. Animal Feed Science and Technology. 238. 9-17. 2018.

Lueschow, S. R., & McElroy, S. J. The Paneth Cell: The Curator and Defender of the Immature Small Intestine. Frontiers in Immunology. 11. 587. 2020.

Zhou, C., Xu, P., Huang, C., Liu, G., Chen, S., Hu, G., Li, G., et al (2020). Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken. Ecotoxicology and Environmental Safety. 188 (30), 109920.

Mert, N., & Yildirim, B. A (2016). Biochemical Parameters and Histopathological Findings in the Forced Molt Laying Hens. Revista Brasileira de Ciência Avícola. 18 (4), 711-718.

Nonose, R., Spadarim, A. P. P., Priolli, D. G., Máximo, F. R., Pereira, J. A., & Martinez, C. A. R (2009). Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats. Acta Cirúrgica Brasileira. 24 (4), 267-275.

NRC (2009). National Research Council. Nutrients requeriments of poultry. 9. ed., D.C.: National Academic Press, Washington. 1994. 155 p. English.

Park, S., Zhen, G., Verhaeghe, C., Nakagami, Y., Nguyevu, L. T., Barczak, A., Kileen, N., et al (2009). The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Proceedings of the National Academy of Sciences. 106 (17), 6950-6955.

Park(a), S. Y., Birkhold, G., Kubena, L. F., Nisbet, D. J., & Ricke, S. C (2004). Review on the Role of Dietary Zinc in Poultry Nutrition, Immunity, and Reproduction. Biological Trace Element Research. 101 (2), 147-163.

Park(b), S. Y., Birkhold, S. G., Kubena, L. F., Nisbet, D. J., & Ricke, S.C (2004). Effects of High Zinc Diets Using Zinc Propionate on Molt Induction, Organs, and Postmolt Egg Production and Quality in Laying Hens. Poultry Science. 83 (1), 24–33.

Pavlova, V., Georgieva, L., Paunova, T., Stoitsova, S., & Nikolova, E (2013). Carbohydrate localization in intestinal glycocalyx. Science & Technologies. 3 (1), 17-21.

Petek, M., Gezen, S. S., Alpay, F., & Cibik, R (2008). Effects of non-feed removal molting methods on egg quality traits in commercial brown egg laying hens in Turkey. Tropical Animal Health and Production. 40 (6), 413-417.

Poloni, V., Magnoli, A., Fochesato, A., Cristofolini, A., Caverzan, M., Merkis, C., Montenegro, M., et al (2020). A Saccharomyces cerevisiae RC016-based feed additive reduces liver toxicity, residual aflatoxin B1 levels and positively influences intestinal morphology in broiler chickens fed chronic aflatoxin B1-contaminated diets. Animal Nutrition. 6 (1), 31-38.

Pongket, P., Romrattanapun, S., Liumsiricharoen. M., Srisai, D., & Suprasert, A (2001). Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat. Kasetsart Journal - Natural Science. 35 (2), 139-143.

Reece, W. O (2017). Dukes fisiologia dos animais domésticos. 13th ed. Rio de Janeiro: Guanabara Koogan. 740p. Portuguese.

Sahin, N., Onderci, M., & Sahin. K (2002). Effects of dietary chromium and zinc on egg production, egg quality, and some blood metabolites of laying hens reared under low ambient temperature. Biological Trace Element Research. 85 (1), 47–58.

Santos, M., Arantes, F. P., Pessali, T. C., & Santos, J. E (2015). Morphological, histological and histochemical analysis of the digestive tract of Trachelyopterusstriatulus (Siluriformes: Auchenipteridae). Zoologia (Curitiba). 32 (4), 296–305.

Shimizu, M (2010). Interaction between Food Substances and the Intestinal Epithelium. Bioscience, biotechnology, and biochemistry. 74 (2), 232-241.

Statistical Analysis System (SAS) Institute (2002) SAS/STAT User’s Guide. Version 8, 6th Edition, SAS Institute, Cary, 112.

Strobel, S., Encarnação, J. A., Becker, N. I & Trenczek, T. E (2015). Histological and Histochemical Analysis of the Gastrointestinal Tract of the Common Pipistrelle Bat (Pipistrellus Pipistrellus). European Journal of Histochemistry.59 (2), 2477.

Sun, H., Tang, J., Yao, X., Yao, X., Wu, Y., Wang, X., & Feng, J (2012). Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers. Tropical Animal Health and Production. 45 (4), 987–993.

Svihus, B (2014). Function of the digestive system. The Journal of Applied Poultry Research. 23 (2), 306-314.

Tarasov. A. I., Semplici, F. L. I. D., Rizzuto, R., Raiver, M. A.; Gilon, P., & Rutter, G.A (2013). Frequency-dependent mitochondrial Ca(2+) accumulation regulates ATP synthesis in pancreatic β cells. Pflügers Archiv European Journal of Physiology. 465 (4), 543-54.

Teixeira, R. S. C., & Cardoso, W.M (2011). Muda forçada na avicultura moderna. Revista Brasileira de Reprodução Animal. 35 (4), 444-455.

Wu, Q. J., Liu, N., Wu, X.H., Wang, G.Y., & Lin, L (2018). Glutamine alleviates heat stress-induced impairment of intestinal morphology, intestinal inflammatory response, and barrier integrity in broilers. Poult Sci. 97 (8), 2675–2683.

Xing, R., Yang, H., Wang, X., Yu, H., Liu, S., & Li, P (2020). Effects of Calcium Source and Calcium Level on Growth Performance, Immune Organ Indexes, Serum Components, Intestinal Microbiota, and Intestinal Morphology of Broiler Chickens. Journal of Applied Poultry Research. 29 (1), 106-120.

Yamauchi, K. (2007) Review of a histological intestinal approach to assessing the intestinal function in chickens and pigs. Animal Science Journal. 8 (4), 356-370.



How to Cite

ARTONI, S. M. B. .; ARAÚJO, D. P. de .; OLIVEIRA, L. P. de .; SANTOS, G. P. .; MASCARENHAS, L. J. S. .; FRANZO, V. S. .; VULCANI, V. A. S. . Histochemistry study of the jejunum and the cecum in commercial laying hens submitted to the different forced molting programs . Research, Society and Development, [S. l.], v. 10, n. 12, p. e191101220211, 2021. DOI: 10.33448/rsd-v10i12.20211. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20211. Acesso em: 26 feb. 2024.



Agrarian and Biological Sciences