Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin

Authors

DOI:

https://doi.org/10.33448/rsd-v10i13.20667

Keywords:

Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.; Arrabidaea chica; Biofilms; Candida albicans; Cytotoxicity; Fibroblasts; Keratinocytes; Polymethylmethacrylate.

Abstract

This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins.

References

Al-Dwairi ZN, Al-Quran FA, Al-Omari OY (2012) The effect of antifungal agents on surface properties of poly(methylmethacrylate) and its relation to adherence of Candida albicans. J Prosthodont Res 56:272-280. https://doi.org/10.1016/j.jpor.2012.02.006

Aligiannis N, Kalpotzakis E, Mitaku S, Chinou IB (2001) Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of Agricultural and Food Chemistry 40:4168–4170.

Aro AA, Simões GF, Esquisatto MAM, Foglio MA, Carvalho JE, Oliveira ALR, Gomes L, Pimentel ER (2012) Arrabidaea chica extract improves gait recovery and changes collagen content during healing of the Achilles tendon. Injury 44:884-892. https://doi.org/10.1016/j.injury.2012.08.055

Aro AA, Freitas KM, Foglio MA et al (2013) Effect of the Arrabidaea chica extract on collagen fiber organization during healing of partially transected tendo. Life Sci. 93(13):799-807. Doi:101016/j.lfs.2013.02.011

Correa RMS, Mota TC, Guimarães AC, Bonfim LT, Burbano RR, Bahia MO (2018) Cytotoxic and genotoxic effects of fluconazole on african green monkey kidney (vero) cell line. Biomed Res Int 2018:1-7. https://doi.org/10.1155/2018/6271547

Cowen LE, Sanglard D, Calabrese D, Sirjusingh C, Anderson JB, Kohn LM (2000) Evolution of drug resistance in experimental populations of Candida albicans. J Bacteriol 182:1515–1522. https://doi.org/10.1128/JB.182.6.1515-1522.2000.

Devia B, Llabres G, Wouters J, Dupont L, Escribano-Bailon MT, Pascual-Teresa S, Angenot L, Tits M (2002) New 3-deoxyanthocyanidins from leaves of Arrabidaea chica. Phytochemical Analyss 13:114-120. https://doi.org/10.1002/pca.632

El Bahra S, Ludwig K, Samran A, Freitag-Wolf S, Kern M (2013) Linear and volumetric dimensional changes of injection-molded PMMA denture base resins. Dent Mater 29:1091–1097. https://doi.org/10.1016/j.dental.2013.07.020

Felipucci DN, Davi LR, Paranhos HF, Bezzon OL, Silva RF, Barbosa Júnior F, Pagnano VO (2011) Effect of different cleansers on the weight and ion release of removable partial denture: an in vitro study. J Appl Oral Sci 19:483–487. http://dx.doi.org/10.1590/S1678-77572011000500008

Fernandes FSF, Pereira-Cenci T, da Silva WJ, Ricomini Filho AP, Straioto FG, Cury AADB (2011) Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins. J Prosthet Dent 105:51-58. https://doi.org/10.1016/S0022-3913(10)60192-8

Freires IA, Bueno-Silva B, Galvão LCC, Duarte MCT, Sartoratto A, Figueira GM, Alencar SM, Rosalen PL (2015) The effect of essential oils and bioactive fractions on Streptococcus mutans and Candida albicans biofilms: Confocal analysis. J Evid Based Complementary Altern Med. Article ID 871316,. http://dx.doi.org/10.1155/2015/871316

Galvão LCC, Furletti VF, Bersan SMF, Cunha MG, Ruiz ALTG, Carvalho JE, Sartoratto A, Rehder VLG, Figueira GM, Duarte MCT, Ikegaki M, Alencar SM, Rosalen PL (2012) Antimicrobial activity of essential oils against streptococcus mutans and their antiproliferative effects. J Evid Based Complementary Altern Med. 40:1–12. http://dx.doi.org/10.1155/2012/751435.

Giordani C, Santin R, Cleff MB (2015) Levantamento de extratos vegetais com ação anti-Candida no período de 2005-2013. Rev Bras Plantas Med 17:175-185. http://dx.doi.org/10.1590/1983-084X/12_072

Glass RT, Conrad RS, Bullard JW, Goodson LB, Mehta N, Lech SJ, Loewy ZG. (2010). Evaluation of microbial flora found in previously worn prostheses from the Northeast and Southwest regions of the United States. J Prosthet Dent 103:384–9. https://doi.org/10.1016/S0022-3913(10)60083-2

Gonçalves LM, Madeira PLM, Diniz RS, Nonato RF, Siqueira FSF, Sousa EM, Farias DCS, Rocha FMG, Rocha CHL, Lago ADN, Monteiro CA (2019) Effect of Terminalia catappa Linn. on biofilms of Candida albicans and Candida glabrata and on changes in color and roughness of acrylic resin. Evid Based Complement Alternat Med 2019:1-8. https://doi.org/10.1155/2019/7481341

Heidrich D, Fortes CBB, Mallmann AT, Vargas CM, Arndt PB, Scroferneker ML (2018) Rosemary, castor oils, and propolis extract: activity against Candida albicans and alterations on properties of dental acrylic resins. J Prosthodont 00:1–6. https://doi.org/10.1111/jopr.12746

Hofling JF, Anibal PC, Obando-Pereda GA, Peixoto IAT, Furletti VF, Foglio MA, Gonçalves RB (2010) Antimicrobial potential of some plant extract again Candida species. Braz J Biol 70:1065-1068. http://dx.doi.org/10.1590/1807-3107BOR-2014.vol28.0017

ISO – 10993-5, International Standard Organization (2009) Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. 3rd ed. Genebra: International Standard Organization.

Jorge MP, Madjarof C, Ruiz ALTG, Fernandes AT, Rodrigues RAF, Souza IMO, Foglio MA, Carvalho JE (2008) Evaluation of wound healing properties of Arrabidaea chica verlot extract. J Ethnopharmacol 118:361-366. https://doi.org/10.1016/j.jep.2008.04.024

Kuhn DM, Chandra J, Mukherjee PK, Ghannoum MA (2002) Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces Infection and Immunity. 70(2):878–888.

Lee JH, El-Fiqi A, Jo JK, Kim DA, Kim SC, Jun SK, Kim HW, Lee HH (2016) Development of long-term antimicrobial poly(methyl methacrylate) by incorporating mesoporous silica nanocarriers. Dent Mater 32:1564-1574. https://doi.org/10.1016/j.dental.2016.09.001

Little JW, Falace DA (1987) Dental management of medically compromised patients. St Louis: CV Mosby CO.

Lorenzi H, Matos FJA (2008) Plantas Medicinais no Brasil: nativas e exóticas. 2ª ed. Nova Odessa, Instituto Plantarum.

Lucena-Ferreira SC, Ricomini-Filho AP, da Silva WJ, Cury JA, Cury AADB (2014) Influence of daily immersion in denture cleanser on multispecies biofilm. Clin Oral Investig 18:2179–2185. https://doi.org/10.1007/s00784-014-1210-9

Madeira PLB, Carvalho LT, Paschoal MAB, de Sousa EM, Moffa EB, da Silva MAS, Tavarez RJR, Gonçalves LM (2016) In vitro effects of lemongrass extract on Candida albicans biofilms, human cells viability, and denture surface. Front Cell Infect Microbiol 6:1-9. https://doi.org/10.3389/fcimb.2016.00071

Mafioleti L, Silva Junior IF, Colodel EM, Flach A, Martins DTO (2013) Evaluation of the toxicity and antimicrobial activity of hydroethanolic extract of Arrabidaea chica (Humb. & Bonpl.) B. Verl. J Ethnopharmacol 150:576-582. https://doi.org/10.1016/j.jep.2013.09.008

Medeiros BJL, Costa KS, Ribeiro JFA, Silva JOC, Barbosa Júnior WLR, Carvalho JCT (2011) Liver protective activity of a hidroethanolic extract of Arrabidaea chica (Humb. and Bonpl.) B. Verl. (pariri). Pharmacognosy Res 3:79-84. https://doi.org/10.4103/0974-8490.81954

Ministério da Saúde (2006) Portaria nº 971, de 3 de maio de 2006. Política Nacional de Práticas Integrativas e Complementares (PNPIC) no Sistema Único de Saúde. http://bvsms.saude.gov.br/bvs/saudelegis/gm/2006/prt0971_03_05_2006.html. Acessado em 10 de fevereiro de 2019.

Moon A, Powers JM, Kiat-Amnuay S (2014). Color stability of denture teeth and acrylic base resin subjected daily to various consumer cleansers. J Esth and Rest Dentistry 26:247-255. https://doi.org/10.1111/jerd.12109.

Moura JS, da Silva WJ, Pereira T, Cury AADB, Garcia RCMR (2006) Influence of acrylic resin polymerization methods and saliva on the adherence of four Candida species. J Prosthet Dent 96: 205-11. https://doi.org/10.1016/j.prosdent.2006.07.004

Nett JE (2014) Future directions for anti-biofilm therapeutics targeting Candida. Expert Rev Anti Infect Ther 12:375–382. https://doi.org/10.1586/14787210.2014.885838

Newman DJ, Cragg GM (2012) Natural Products as Sources of New Drugs over the 30 Years from 1981 to 2010. J Nat Prod 75:311–335. https://doi.org/10.1021/np200906s

Oliveira DPC, Borrás MRL, Ferreira CLF, López-Lozano JL (2009) Atividade anti-inflamatória do extrato aquoso de Arrabidaea chica (Humb & Bonpl.) B. Verl. sobre o edema induzido por venenos de serpentes amazônicas. Rev Bras Farmacogn 19:643-649. http://dx.doi.org/10.1590/S0102-695X2009000400024

Orsi IA, Junior AG, Villabona CA, Fernandes FH, Ito IY (2011) Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerized acrylic resin. Gerodontology 28:253–257. https://doi.org/10.1111/j.1741-2358.2010.00400.x

Padilha DM, Hugo FN, Hilgert JB, Dal Moro RG (2007) Hand function and oral hygiene in older institutionalized Brazilians. J Am Geriatr Soc 55:1333–8. https://doi.org/10.1111/j.1532-5415.2007.01278.x

Paranhos HF, Davi LR, Peracini A, Soares RB, Lovato CH, Souza RF (2009) Comparison of physical and mechanical properties of microwavepolymerized acrylic resin after disinfection in sodium hypochlorite solutions. Braz Dent J 20:331-335. http://dx.doi.org/10.1590/S0103-64402009000400012

Paranhos HF, Peracini A, Pisani MX, Oliveira VC, Souza RF, Silva-Lovato CH (2013) Color stability, surface roughness and flexural strength of an acrylic resin submitted to simulated overnight immersion in denture cleansers. Braz Dent J 24:152–156. http://dx.doi.org/10.1590/0103-6440201302151

Paranhos HF, Bezzon OL, Davi LR, Felipucci DN, Silva CH, Pagnano VO (2014) Effect of cleanser solutions on the color of acrylic resins associated with titanium and nickel-chromium alloys. Braz Oral Res 28:1–7.

Porta SRS, de Lucena-Ferreira SC, da Silva WJ, Cury AADB (2015) Evaluation of sodium hypochlorite as a denture cleanser: a clinical study. Gerodontology 32:260-266. https://doi.org/10.1111/ger.12104

Santos VC, Garcia AL, Richter MF, Guecheva TN, Henriques JA, Ferraz AB, Picada JN (2013) Evaluation of the mutagenicity and genotoxicity of Arrabidaea chica Verlot (Bignoneaceae), an Amazon plant with medicinal properties. J Toxicol Environ Health A 76:381-390.https://doi.org/10.1080/15287394.2012.761947.

Solana C, Ferrer-Luque CM, Wang X, Baca P, Ruiz-Linares M, Cabrera T. Cytotoxic effects of alkaline tetrasodium EDTA irrigating solutions. J Oral Sci. 2020 Jun 23;62(3):285-287. doi: 10.2334/josnusd.19-0234. Epub 2020 May 31. PMID: 32475864.

Tan CM, Tsoi JK, Seneviratne CJ, Matinlinna JP (2014) Evaluation of the Candida albicans removal and mechanical properties of denture acrylics cleaned by a low-cost powered toothbrush. J Prosthodont Res 58:243–51. https://doi.org/10.1016/j.jpor.2014.06.002

Van Den Berg ME (1993) Plantas medicinais na Amazônia, 2ª Ed. Belém: CNPq/Programa Trópico Úmido.

Vieira APC, Senna PM, Da Silva WJ, Del Bel Cury AA (2010) Long-term efficacy os denture cleansers in preventing Candida spp. biofilm recolonization on liner surface. Braz Oral Res 24: 342–348.

Webb BC, Thomas CJ, Whittle T (2005) A 2-year of Candida-associated denture stomatitis treatment in aged care subjects. Gerodontology 22:168–176. https://doi.org/10.1111/j.1741-2358.2005.00065.x

Wen D, Li C, Di H, Liao Y, Liu H, Universal A (2005). HPLC method for the determination of phenolic acids in compounds herbal medicines. J Agric Food Chem. 53(17): 6624-9. Doi:10.1021/jf0511291

Wieckiewicz M, Opitz V, Richter G, Boening KW (2014) Physical properties of polyamide-12 versus PMMA denture base material. Biomed Res Int 2014:1-8. http://dx.doi.org/10.1155/2014/150298

Wiziack Zago PM, Oliveira Sousa IM, Servat-Medina L, Jorge MP, Lima Neto LG, Hass V, Li X, Tasca Gois Ruiz AL, Saxena D, Foglio MA. Standardized Arrabidaea chica Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts. Clin Cosmet Investig Dent. 2020 Aug 11;12:327-333. doi: 10.2147/CCIDE.S259158. PMID: 32848479; PMCID: PMC7429228.

Zomorodian K, Haghighi NN, Rajaee N, Pakshir K, Tarazooie B, Vojdani M, Sedaghat F, Vosoghi M (2011) Assessment of Candida species colonization and denture-related stomatitis in complete denture wearers. Med Mycol 49:208–211.https://doi.org/10.3109/13693786.2010.507605

Published

12/10/2021

How to Cite

BRANDÃO, C. M. M. .; HASS, V. .; ZAGO, P. W. .; GALVÃO, L. C. de C. .; DIBAI, D. B. .; SILVA, F. B. .; MALHEIROS, A. S. .; SIMAMOTO JÚNIOR, P. C. .; TAVAREZ, R. R. de J. . Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin. Research, Society and Development, [S. l.], v. 10, n. 13, p. e292101320667, 2021. DOI: 10.33448/rsd-v10i13.20667. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20667. Acesso em: 4 dec. 2021.

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Section

Health Sciences