Combination of sugars, lipoproteins and centrifugation improve dairy goat sperm viability submitted to cryopreservation
Keywords:Freezing; freezing; Mitochondria; phospholipase A; Phospholipase A; mitochondria; Seminal plasma.; seminal plasma
The aim of the present study was to evaluate the effects of different cryoprotectants and semen centrifugation on the kinetic parameters and membrane integrity of cryopreserved goat semen. Four buck goats were used, and their semen was collected using an artificial vagina. After semen collection and approval, six pools were formed, and each pool was divided into eight aliquots. The plasma was removed from four aliquots by centrifugation (1200 g/10 min) and subsequently diluted; the remaining four aliquots were diluted in standard Tris-egg yolk (SYE), standard milk (SME), test Tris-egg yolk (TYE) and test milk (TME) extenders without removing the seminal plasma. After dilution, samples were loaded into straws (0.25 mL), frozen and stored at -196 °C. The samples were thawed (37 °C/30 s) and evaluated immediately and two hours after thawing to determine the kinetics and integrity of plasma and mitochondrial membranes. A difference (p< 0.05) in the maintenance of sperm kinetics, plasma membrane integrity and mitochondrial potential was observed between the centrifuged and non-centrifuged groups and between the extenders at different time points. We concluded that the use of centrifugation to remove seminal plasma positively affects the semen evaluation parameters and that the yolk extender is more efficient when applied with different cryopreservation techniques, preserving the desirable traits after the cryopreservation of goat semen.
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Copyright (c) 2021 Aline Francelina de Queiros; Camilla Flávia Avelino de Farias; Alex Souza Rique; André Luiz Pereira Tork; Carlos Augusto Alanis Clemente; Maria Madalena Pessoa Guerra; Sildivane Valcácia Silva
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