Surface analysis of latex and silicone pacifier nipples after exposure to acidic artificial saliva: a pilot study
DOI:
https://doi.org/10.33448/rsd-v10i16.23074Keywords:
Pacifiers; Surface properties; Latex; Silicones.Abstract
Objective: To measure surface changes of pacifier nipples after immersion in acidic artificial saliva. Methods: Two types of pacifier nipples (latex and silicone) were selected. Five copies of each type were used. The protocol consisted of immersion in acidic artificial saliva (pH = 5.97) for 16 days. The nipples of the pacifier remained in direct contact with saliva in an individualized, opaque and closed container in a bacteriological incubator at 37ºC. The samples were examined before and after exposure to saliva through qualitative (based on images obtained in a 100 mm macro lens and three-dimensional images with 10% amplification obtained in the profilometry) and quantitative (roughness data obtained in non-contact 3D optical profilometry). Data were obtained in duplicate and analyzed using descriptive and inferential statistics. The findings were tested for normality (Shapiro-Wilk). Paired t-test or Wilcoxon was used to compare before and after roughness data. Results: In the image analysis, it was observed that only the surface of the latex pacifier's nipples showed an irregular appearance after exposure to saliva. In the quantitative evaluation, no differences were found between the roughness values before and after (p>0.05). Conclusion: This pilot study demonstrated qualitative changes in the surface of latex pacifiers. However, it was not able to demonstrate statistically significant differences for the roughness parameters before and after exposure to acidic artificial saliva in the nipples of latex and silicone pacifiers.
References
Aarts, C., Hörnell, A., Kylberg, E., Hofvander, Y., & Gebre-Medhin, M. (1999). Breastfeeding Patterns in Relation to Thumb Sucking and Pacifier Use. Pediatrics, 104(4), e50.
Anselme, K., Davidson, P., Popa, A. M., Giazzon, M., Liley, M., Ploux, L. (2010). The interaction of cells and bacteria with surfaces structured at the nanometre scale. Acta Biomaterialia, 6(10), 3824-3846.
Barbosa-Cesnik, C., Farjo, R. S., Patel, M., Gilsdorf, J., McCoy, S. I., Pettigrew, M. M., Marrs, C., Foxman, B. (2006). Predictors for Haemophilus influenzae Colonization, Antibiotic Resistance and for Sharing an Identical Isolate Among Children Attending 16 Licensed Day-Care Centers in Michigan. The Pediatric Infectious Disease Journal, 25(3):219-23.
Bezerra, V. M., Magalhães, E. I. S., Pereira, I. N., Gomes, A. T., Netto, M. P., Rocha, D. S. (2019). Prevalência e fatores determinantes do uso de chupetas e mamadeiras: um estudo no sudoeste baiano. Revista Brasileira de Saúde Materno Infantil, 19(2), 323-333.
Brook, I., & Gober, A. E. (1997). Bacterial colonization of pacifiers of infants with acute otitis media. The Journal of Laryngology and Otology, 111(7):614-5.
Castilho, S. D., & Rocha, M. A. M. (2009). Pacifier habit: history and multidisciplinary view. Jornal de Pediatria (Rio J), 85(6), 480-9.
Castilho, S. D., Casagrande, R. C., Rached, C. R., & Nucci, L. B. (2012). Prevalence of pacifier among breastfed and not breastfed infants attending a university hospital. Revista Paulista de Pediatria, 30(2), 166-72.
Comina, E., Marion, K., Renaud, F. N. R., Dore, J., Bergeron, E., Freney, J. (2006). Pacifiers: A microbial reservoir. Nursing & Health Sciences, 8(4), 216-23.
Gadelmawla, E. S., Koura, M. M., Maksoud, T. M. A., Elewa, I. M., & Soliman, H. H. (2002). Roughness parameters. Journal of Materials Processing Technology, 123, 133-145.
Isa, S. Z., Yahya, R., Hassan, A., & Tahir, M. (2007). The influence of temperature and reaction time in the degradation of natural rubber latex. The Malaysian Journal of Analytical Sciences, 11(1), 42-7.
Moon, R. Y., & AAP TASK FORCE ON SUDDEN INFANT DEATH SYNDROME. (2016). SIDS and Other Sleep-Related Infant Deaths: Evidence Base for 2016 Updated recommendations for a Safe Infant Sleeping Environment. Pediatrics, 138(5), e20162940.
Nelson-Filho, P., Louvain, M. C., Macari, S., Lucisano, M. P., Silva, R. A. B., Queiroz, A. M., Gaton-Hernández, P., Silva, L. A. B. (2015). Microbial contamination and disinfection methods of pacifiers. Journal of Applied Oral Science, 23(5):523-8.
Park, J. W., Song, C. W., Jung, J. H., Ahn, S. J., & Ferracane, J. L. (2012). The effects of surface roughness of composite resin on biofilm formation of streptococcus mutans in the presence of saliva. Operative Dentistry, 37(5), 532-539.
Pedroso, R. S., & Siqueira, R. V. (1997). A study on protozoan cysts, helminth eggs and larvae in pacifiers. Jornal de Pediatria (Rio J), 73(1), 21-5.
Phinyocheep, P., Phetphaisit, C. W., Derouet, D., Campistron, I., & Brosse, J. C. (2005). Chemical degradation of epoxidized natural rubber using periodic acid: preparation of epoxidized liquid natural rubber. Journal of Applied Polymer Science, 95, 6–15.
Pyati, S. A., Kumar, R. N., Kumar, V., Kumar, N. H. P., & Reddy, K. M. P. (2018). Salivary flow rate, pH, buffering capacity, total protein, oxidative stress and antioxidant capacity in children with and without dental caries. The Journal of Clinical Pediatric Dentistry, 42(6), 445-449.
Rovers, M. M., Numans, M. E., Langenbach, E., Grobbee, D. E., Verheij, T. J. M., Schilder, A. G. M. (2008). Is pacifier use a risk factor for acute otitis media? A dynamic cohort study. Family Practice, 25(4), 233-6.
Schlueter, N., Amaechi, B. T., Bartlett, D., Buzalaf, M. A. R., Carvalho, T. S., Ganss, C. … Lippert, F. (2020) Terminology of erosive tooth wear: consensus report of a workshop organized by the orca and the cariology research group of the IADR. Caries Research, 54(1), 2-6.
Silveira, L. C., Charone, S., Maia, L. C., Soares, R. M. A., & Portela, M. B. (2009). Biofilm formation by Candida species on silicone surfaces and latex pacifier nipples: an in vitro study. The Journal of Clinical Pediatric Dentistry, 33(3), 235–240.
Sio, J. O., Minwalla, F. K., George, R. H., & Booth, I.W. (1987). Oral candida: is dummy carriage the culprit? Archives of Disease in Childhood, 62(4), 406-8.
Sterzenbach, T., Helbig, R., Hannig, C., & Hannig, M. (2020). Bioadhesion in the oral cavity and approaches for biofilm management by surface modifications. Clinical Oral Investigations, 24(12), 4237-4260.
Tang, H., Cao, T., Liang, X., Wang, A., Salley, S. O., McAllister, J 2nd., Ng, K. Y. (2009). Influence of silicone surface roughness and hydrophobicity on adhesion and colonization of Staphylococcus epidermidis. Journal of Biomedical Materials Research. Part A, 88(2), 454-63.
Yuan, C., Wang, X., Gao, X., Chen, F., Liang, X., Li, D. (2016). Effects of surface properties of polymer-based restorative materials on early adhesion of Streptococcus mutans in vitro. Journal of Dentistry, 54, 33-40.
Zhou, Z., Liu, F., Shen, S., Shang, L., Shang, L., Wang, X. (2016). Prevalence of and factors affecting malocclusion in primary dentition among children in Xi’an, China. BMC Oral Health, 16(1), 91.
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