In vitro culture establishment of Hibiscus sabdariffa L.: obtaining seedlings and induction of callus under different culture conditions aiming at biomass accumulation
DOI:
https://doi.org/10.33448/rsd-v10i15.23114Keywords:
Callogenesis; Culture media; Biotecnology; Growth regulators.Abstract
Hibiscus sabdariffa L., is a plant that belong to the family Malvaceae Juss., popularly known as vinagreira, the species has pharmacological importance. The in vitro culture of callus represents an important resource for the continuous production of high quality medicinal compounds, being possible the production of biomass with compounds of medicinal interest. The present work aimed to verify the necessary cultivation conditions for the highest production of wet callogenic mass of explants (hypocotyls and roots). The explants were inoculated in different basic culture media (MS and B5) supplemented with different concentrations of growth regulators and kept in a B.O.D incubator at 30ºC, photoperiod of 16 h of light. Callus production was evaluated by measuring the wet weight of callus after 120 days of cultivation. Based on the results obtained in the study, it can be observed that the proliferation of H. sabdariffa, callus occurred regardless of the type of explants, basal media or concentrations of growth regulators, 2,4-D and BAP, but the best results for the production of callogenic masses of hypocotyls and roots were in MS medium supplemented with 0,1 mg.L-1 of 2,4-D in combination with 0,1 mg.L-1 of BAP (T2), presenting averages of callogenic masses from hypocotyl explants (7,43 ± 0.19 g) and roots (6,75 ± 0.07 g). The results show that in vitro techniques have advantages of biomass production in sterile and controlled environmental conditions, allowing a better control of the development processes and production of tissues rich in bioactive compounds.
References
Alvarenga, I. C. A., Pacheco, F. V., Silva, S. T., Bertolucci, S. K. V. & Pinto, J. E. B. P. (2015). In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 299-308.
Andrade, S. R. M. (2002). Princípios da cultura de tecidos vegetais. (58a ed.), Embrapa cerrados, 16p.
Ariati, S. N., Waeniati, M. & Suwastika, I. N. (2012). Induksi kalus tanaman kakao (Theobroma cacao L.) pada media MS dengan penambahan 2,4-D, BAP dan air kelapa. Natural Science: Journal of Science and Technology, 1(1), 74-84.
Ashrafadeh, S., Gaw, S., Glover, C. N. & Leung, D. W. M. (2015). Differential cadmium resistance of two morphologically distinct types of potato (Solanum tuberosum) callus. Biologia (Bratislava), 70(5), 581-587.
Avilés, F., Rios, D., González, R. & Sánchez-Olate, M. (2009). Effect of culture medium in callogenesis from adult walnut leaves (Juglans regia L.). Chilean Journal of Agricultural Research, 69(3), 460-467.
Barreiro, E. J. & Bolzani, V. S. (2009). Biodiversidade: fonte potencial para a descoberta de fármacos. Química Nova, 32(3), 679-688.
Bekheet, S. A., El-Bahr, M. K., Sanaa, A., Ali, S. A. & Hamed, M. A. (2014). Callus production of globe artichoke and milk thistle: in vitro hypolipidemic and antioxidant activities. World Journal of Pharmaceutical Research, 3, 1-17.
Birari, R. B. & Bhutani, K. K. (2007). Pancreatic lipase inhibitorsfrom natural sources: unexplored potential. DrugDiscovery Today, 12(19/20), 879-89.
Borrás-Linares, I., Fernández-Arroyo, S., Arráez-Roman, D., Palmeros-Suárez, P. A., Val-Díaz, R. D., Andrade-Gonzáles, I., Fernández-Gutiérrez, A., Gómez-Leyva, J. F. & Segura-Carretero, A. (2015). Characterization of phenolic compounds, anthocyanidin, antioxidante and antimicrobial activity of 25 varieties of Mexican Roselle (Hibiscus sabdariffa). Industrial Crops and Products 69, 385-394.
Brasil (2009). Regras para análises de sementes do Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. MAPA/ ACS.
Cruz, M., Acosta, M., Capote, A., Leiva, M. & Alvarado, Y. (2003). Efectos del carbendazim para el control de Colletotrichum sp., contaminante del establecimiento in vitro de callos de café. Biotecnologia vegetal, 3(2), 111-113.
De Sá Junior, P. F., Muniz, E. B., Pereira, N. A. & Oliveira, M. A. S. (2016). Atividade antimicrobiana in vitro dos extratos aquosos, hidroalcoólicos e alcoólicos de espécies da família Anacardiaceae. Revista de Ciências Médicas e Biológicas, 15(1), 56-61.
De Souza Eller, S. C. W., Feitosa, V. A., Arruda, T. A., Antunes, R. M. P. & Catão, R. M. R. (2015). Avaliação antimicrobiana de extratos vegetais e possível interação farmacológica in vitro. Journal of Basic and Applied Pharmaceutical Sciencies, 36(1), 131-136.
Efferth, T. (2019). Biotechnology Applications of Plant Callus Cultures. Engineering, 5(1), 50-59.
Eichelberger, L. & Moraes, D. M. (2001). Preparo de sementes de quiabo (Abelmoschusesculentus (L.) Moench) para o teste tetrazólio. Revista Brasileira de Sementes, 23(1), 154-158.
Evans, D. E., Coleman, J. O. D. & Kearns, A. (2003). Plant Cell Culture, BIOS Scientific Publishers.
Firmo, W. C. A, Menezes, V. J. M, Passos, C. E. C, Dias, C. N, Alves, L. P. L, Dias, I. C. L, Santos Neto, M. & Olea, R. S. G. (2011). Contexto histórico, uso popular e concepção científica sobre plantas medicinais. Caderno de pesquisa, 18
França, S. C. (2017). Bioprocessos inovadores para produção de metabólitos ativos de plantas. In: Simões, C. M. O., Schenkel, E. P., Mello, J. C. P., Mentz, L. A., Petrovick P. R. (editores). Farmacognosia do produto natural ao medicamento. Artmed, 39-52.
Fumagali, E., Gonçalves, R. A. C., Machado, M. F. P. S., Vidoti, G. J. & Oliveira, A. J. B. (2008). Produção de metabólitos secundários em cultura de células e tecidos de plantas: o exemplo dos gêneros Tabernaemontana e Aspidosperma. Revista Brasileira de Farmacognosia, 18(4), 627-641.
Gamborg, O. L., Murashige, T., Thorpe, T.A. & Vasil, I. K. (1976). Plant tissue culture media. In Vitro Cellular & Developmental Biology – Plant, 12, 473-478.
Gamborg, O., Miller. R. & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50, 151-158.
Grzegorczyk-Karolak, I., Kuźma, L. & Wysokińska, H. (2015). The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina. Plant Cell, Tissue and Organ Culture (PCTOC), 122, 699-708.
Hartmann, H. T., Kester, D. E., Davies, Jr. & Geneve, R. L. (2004). Plant propagation: principles and practicas. New York: Prentice Hall, (8a ed.), 880p.
Hayati, S. K., Nurchayati, Y. & Setiari, N. (2010). Induksi kalus dari hipokotil Alfafa (Medicago sativa) secara in vitro dengan penambahan benzyl amino purine (BAP) dan a-naphtalene acetic acid (NAA). Bioma, 12(1), 6-12.
Hoesen, D. S. H. & Sukamto, W. La. (2008). Induksi Kalus dan Organogenesis Kultur In Vitro Dendrobium lineale Rolfe. Berita Biologi, 9(3), 333-341.
Hussain, M. S., Fareed, S., Ansari, S., Rahman, M. A., Ahmad, I. Z. & Saeed, M. (2012). Current approaches toward production of secondary plant metabolites. Journal of Pharmacy and Bioallied Sciences, 4(1), 10-20.
Ikeuchi, M., Sugimoto, K. & Iwase, A. (2013). Plant callus: mechanisms of induction and repression. The Plant Cell, 25, 3159-3173.
Ishii, Y., Takamura, T., Goi, M. & Tanaka, M. (2004). Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Reports, 17(6), 446-450.
Jahan, M. T., Islam, M. R., Khan, R., Mamun, A. N. K., Ahmed, G. & Hakim, L. (2009). In Vitro Clonal Propagation of Anthurium (Anthurium andraeanum L.) using Callus Culture. Plant Tissue Culture and Biotechnology, 19(1), 61-69.
Jamshidi-Kia, F., Lorigooini, Z. & Amini-Khoei, H. (2018). Medicinal plants: Past history and future perspective. Journal of HerbMed Pharmacology, 7(1), 1-7.
Kanashiro, S. et al. (2007). Efeitos de diferentes concentrações de nitrogênio no crescimento de Aechmea blanchetiana (Baker) L.B. Sm. cultivada in vitro. Hoehnea, 34(1), 59-66.
Kapepula, P. M., Kabamba, N. N., Tshisekedi, T. P., Tsumbu C., Franck, T., Mouithys-Mickalad, A., Mumba, D., Tshala-Katumbay, D., Serteyn, D., Tits, M., Angenot, L., Kalenda, P. D. T. & Frédérich, M. (2017). Comparison of metabolic profiles and bioactivities of the leaves of three edible congolese Hibiscus species. Natural Product Research, 31(24), 2885-2892.
Karuppusamy S. A. (2009). review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plant Research, 3(13), 1222-39.
Kinupp, V. F., Lorenzi, H. Plantas Alimentícias Não Convencionais (PANC) no Brasil: guia de identificação, aspectos nutricionais e receitas ilustradas. Nova Odessa, SP: Instituto Plantarum de Estudos da Flora, 768p.
Kutchan, T. M. (2001). Ecological arsenal and developmental dispatcher: the paradigm of secondary metabolism. Plant Physiology, 25(1), 58-60.
Lakshmanan, P. & Taji, A. (2000). Somatic embryogenesis in leguminous plants. Plant Biology, 2, 136-148.
López-Laredo, A. R., Ramírez-Flores, F. D., Sepúlveda-Jiménez, G. & Trejo-Tapia, G. (2009). Comparison of metabolite levels in callus of Tecoma stans (L.) Juss. ex Kunth. cultured in photoperiod and darkness. In Vitro Cellular & Developmental Biology, 45, 4-58.
Lorenzi, H. & Matos, F. J. A. (2021). Plantas medicinais no Brasil. Jardim Botânico Plantarum. 576p.
Mansuroglu, S. & Gurel, E. (2001). Micropropagation. In: Babaoglu, M., Gurel, E., Ozcan, S. (editores), Plant Biotechnology, Volume I, Tissue Culture and Its Applications, Selcuk University Press, Konya, Turkey (in Turkish). 262-281.
Manuhara, Y. S. W. (2014). Kapita selekta kultur jaringan tumbuhan. Airlangga University Press.
Mercier, H. & Kerbauy, G. B. (1998). Endogenous IAA and Cytokinin levels in bromeliad shoots as influenced by glutamine and ammonium nitrate treatments. Revista Brasileira de Fisiologia Vegetal, Brasília, 10(3), 225-228.
Morales-Rubio, M. E.., Espinosa-Leal, C. & Garza-Padrón, R. A. (2016). Cultivo De Tejidos Vegetales Y Su Aplicación En Productos Naturales. In: Rivas-Morales C., Oranday-Cardenas, M. A., Verde-Star, M. J (Eds). Investigación en plantas de importancia médica. 1st edn. OmniaScience, Barcelona, p 351-410.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-479.
Murthy, H. N., Lee, E. J. & Paek, K. Y. (2014). Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture (PCTOC), 118, 1-16.
Nagella, P. & Murthy, H. N. (2010). Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A. Bioresource Technology, 101(17), 6735-6739.
Nakasha, J. J., Sinniah, U. R., Kemat, N. & Mallappa, K. S. (2016). Induction, Subculture Cycle, and Regeneration of Callus in Safed Musli (Chlorophytum borivilianum) using Different Types of Phytohormones. Pharmacognosy Magazine, 12(4), 460-464.
Nasution, N. H. & Nasution, I. W. (2019). The effect of plant growth regulators on callus induction of mangosteen (Garcinia mangostana L.). IOP Conference Series: Earth and Environmental Science, 305.
Naveed, M., Hejazi, V., Abbas, M., Kamboh, A. A., Khan, G. J., Shumzaid, M., Ahmad, F., Babazadeh, D., Fangfang, X., Modarresi-Ghazani, F., Wenhua, L. & Xiaohui, Z. (2018). Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomedicine & Pharmacotherapy, 97, 67-74.
Ngomuo, M. S., Mneney, E. & Ndakidemi, P. (2014). The in vitro propagation techniques for producing banana using shoot tip cultures. American Journal of Plant Sciences, 5, 1614-1622.
Oliveira, N. C. C., Lopes, P. S. N., Ribeiro, L. M., Mercandante-Simões, M. O., Oliveira, L. A. A. & Silvério, F. O. (2013). Seed structure, germination, and reserve mobilization in Butia capitata (Arecaceae). Trees, 27(6), 1633-1645.
Pawar B., Prashant, K., Bahurupe, J., Jadhav, A., Anil, K. & Pawar, S. (2015). Proline and Glutamine Improve in vitro Callus Induction and Subsequent Shooting in Rice. Rice Science, 22, 283-289.
Rahman, Z. A., Noor, E. S., Ali, M. S. M., Mirad, R. & Othman, A. N. (2015). In vitro micropropagation of a valuable medicinal plant Plectranthus amboinicus. American Journal of Plant Sciences, 6, 1091-1097.
Shekhawat, M. & Manokari, M. (2016). Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L. Physiology and Molecular Biology of Plants, 22.
Shirin, F., Hossain, M., Kabir, M. F., Roy, M. & Sarker, S. R. (2007). Callus Induction and Plant Regeneration from Internodal and Leaf Explant of Four Potato (Solanum tuberosum L.) Cultivars. World Journal of Agricultural Sciences, 3(1), 1-6.
Shukla, R., Dube, A. & Koshy, E. (2014). Production of high quality embryogenic callus of rice. International Journal of Life Science, 9, 1077-1080.
Sitorus, E. N., Hastuti, E. D. & Setiari, N. (2011). Induksi kalus binahong (Basella rubra L.) secara in vitro pada media Murashige&Skoog dengan konsentrasi sukrosa yang berbeda. Bioma, 13(1), 1-7.
Sobrinho, A. C. G. & Santos, A. S. (2020). Estado da arte: Hibiscus sabdariffa Linn, aspectos químicos, farmacológicos e novas perspectivas de abordagens. Revista Científica Multidisciplinar Núcleo do Conhecimento, 5(5), 21-40.
Sobrinho, A. C. G., Santos, A. S. & Corpes, R. S. (2020a). Aspectos botânicos, morfológicos, germinação e desenvolvimento de plântulas de Hibiscus sabdariffa L. In: Matos, R. R. S. S., Oliveira, A. R. F., Machado, F. G. A. (org.). Floricultura, Plantas Ornamentais e Cultura de Tecidos de Plantas. Ponta Grossa: Atena, 44-55.
Sobrinho, A. C. G., Santos, A. S. & Corpes, R. S. (2020b). Contaminantes na cultura assimbiótica de Hibiscus sabdariffa L. em diferentes concentrações de meios nutritivos e condições de luminosidade. In: JASPER, M. (org.). Aspectos Fitossanitários da Agricultura. Ponta Grossa: Atena, 2020b. 15-22.
Staba, E. J. (1980). Plant tissue culture as a source of biochemical, CRC Press, Florida.
Tambor, J., Cincotto, M. G. J. A., Leone, B. A. & Isaac, V. F. M. (2016). Avaliação da atividade antioxidante e fotoprotetora do extrato de cladódios de pitaya (Hylocereus undatus). Journal of Basic and Applied Pharmaceutical Sciencies, 37(1).
Verma, S. K., Das, A. K., Cingoz, G. S., Uslu, E. & Gurel, E. (2016). Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species. Biotechnol Rep (Amst), 10, 66-74.
Wani, M. & Snehal, P. N. M. (2010). Callus induction studies in Tridax procumbens L., International Journal of Biotechnology Applications, 2(1), 11-14.
Zouzou, M., Kouadio, Y. J., Koné, M., Kouakou, T. H. & Dénézon, D. O. (2000). Callogenèse chez Gossypium hirsutum L.: effets cultivar, conditions de culture et type de matériel. Biot Rev Int Sci Vie Terre, 1(1), 48-5.
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