Bronchopneumonia in sheep associated with Providencia stuartii

Authors

DOI:

https://doi.org/10.33448/rsd-v10i16.23295

Keywords:

Microbiology; Pathology; Pneumonia; Sequencing; Sheep.

Abstract

Bacteria of the genus Providencia are opportunistic pathogens in humans, widely distributed in the environment and associated with greater resistance to antibiotics, and this being uncommon the association with clinical diseases. This study reports the isolation of P. stuartii in two sheep that presented clinical signs of pneumonia. At necropsy there was severe and acute fibrinopurulent bronchopneumonia. Histologically, there were infiltrated neutrophils and fibrin in the alveolar lumen, and the alveolar septa presented multifocal thickening with moderate proliferation of pneumocytes and mononuclear interstitial infiltrate. Providencia sp. was isolated in the microbiological tests of the lung and tracheal secretions. The isolate was subjected to DNA extraction, polymerase chain reaction (PCR) for the 16SrRNA gene and sequencing of genomic DNA, which demonstrated 100% homology with P. stuartii. This is the first report of the presence of this microorganism as a cause of interstitial and fibrinopurulent bronchopneumonia in sheep. Therefore, it is suggested that epidemiological surveillance strategies should be carried out in animals to better understand their role in the dissemination of this pathogen.

References

Ackermann, M. R., & Brogden, K. A. (2000). Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica. Microbes and Infection, 2(9), 1079-1088. doi:10.1016/S1286-4579(00)01262-4.

Almeida, F. S., Rigobelo, E. C., Marin, J. M., Maluta, R. P., & Ávila, F. A. (2007). Diarréia suína: estudo da etiologia, virulência e resistência a antimicrobianos de agentes isolados em leitões na região de Ribeirão Preto-SP, Brasil. ARS Veterinaria, 23(3), 151-157. doi:10.15361/2175-0106.2007v23n3p151-157

Azizi, S., Korani, F. S., & Oryan, A. (2013). Pneumonia in slaughtered sheep in south-western Iran: pathological characteristics and aerobic bacterial aetiology. Veterinaria Italiana, 49(1), 109-118. PMID: 23564592

Barbour, E. K., Hajj, Z. G., Hamadeh, S., Shaib, H. A., Farran, M. T., Araj, G., Faroon, O., Barbour, K. E., Jirjis, F., Azhar, E., Kumosani, T., & Harakeh, S. (2012). Comparison of phenotypic and virulence genes characteristics in human and chicken isolates of Proteus mirabilis. Pathogens and global health, 106(6), 352–357. doi:10.1179/2047773212Y.0000000042

Barl, P., Bedenic, B., Sardelic, S., Uzunovic, S., Vranes, J., & Plecko, V. (2012). Spread of CTX-M-15 positive Providencia spp. causing urinary tract infections at the University Hospital Split in Croatia. Medicinski Glasnik (Zenica), 9(2), 317-324. PMID: 22926370

Bell, S. (2008). Respiratory disease in sheep: 1. Differential diagnosis and epidemiology. In Practice, 30(4), 200–207. doi:10.1136/inpract.30.4.200

Brogden, K. A., Lehmkuhl, H. D., & Cutlip, R. C. (1998). Pasteurella haemolytica complicated respiratory infections in sheep and goats. Veterinary research, 29, 233-254. PMID: 9689740

Carmo, P., Uzal, F. A., Pedroso, P., & Riet-Correa, F. (2020). Conidiobolomycosis, cryptococcosis, and aspergillosis in sheep and goats: a review. Journal of veterinary diagnostic investigation official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 32(6), 826–834. doi:10.1177/1040638720958338

Chakraborty, S., Kumar, A., Tiwari, R., Rahal, A., Malik, Y., Dhama, K., Pal, A., & Prasad, M. (2014). Advances in diagnosis of respiratory diseases of small ruminants. Veterinary Medicine International, 2014. doi:10.1155/2014/508304.

Chamberland, R. R., McElvania, T. E., Burnham, C. A., & Kennedy, D. J. (2013). Renal abscess caused by a Providencia stuartii isolate biochemically misidentified as Pasteurella. Journal of clinical microbiology, 51 (8), 2775–2777. doi:10.1128/JCM.00937-13.

Cid, D., García-Alvarez, A., Domínguez, L., Fernández-Garayzábal, J.F., & Vela, A.I. (2019). Pasteurella multocida isolates associated with ovine pneumonia are toxigenic. Veterinary microbiology, 232, 70–73. doi:10.1016/j.vetmic.2019.04.006

Crane, E. S., Shum, M., & Chu, D. S. (2016). Case report: Providencia stuartii conjunctivitis. Journal of ophthalmic inflammation and infection, 6(1), 29. doi:10.1186/s12348-016-0097-9.

DI, H., Liang, S., Li, Q., Shi, L., Shima, A., Meng, H., Yan, H., & Yamasaki, S. (2018). Providencia in retail meats from Guangzhou, China and Osaka, Japan: prevalence, antimicrobial resistance and characterization of classes 1, 2 and 3 integrons. The Journal of veterinary medical science, 80(5), 829–835. doi:10.1292/jvms.18-0037

Farmer, J. J., 3rd, Davis, B. R., Hickman-Brenner, F. W., McWhorter, A., Huntley-Carter, G. P., Asbury, M. A., Riddle, C., Wathen-Grady, H. G., Elias, C., & Fanning, G. R. (1985). Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. Journal of clinical microbiology, 21(1), 46–76. doi:10.1128/jcm.21.1.46-76.1985

George, E. A., Kornik, R., & Robinson-Bostom, L. (2020). Providencia stuartii septic vasculitis. JAAD case reports, 6(5), 422–425. doi:10.1016/j.jdcr.2020.02.043

Glendinning, L., Collie, D., Wright, S., Rutherford, K.M.D., & McLachian, G.(2017). Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs. Microbiome, 145 (5), 2-9. doi:10.1186/s40168-017-0364-5.

Hussain, R., Mahmood, F., Ali, H. M., & Siddique, A. B. (2017). Bacterial, PCR and clinico-pathological diagnosis of naturally occurring pneumonic pasturellosis (mannheimiosis) during subtropical climate in sheep. Microbial pathogenesis, 112, 176–181. doi:10.1016/j.micpath.2017.09.061

Iwata, S., Tada, T., Hishinuma, T., Tohya, M., Oshiro, S., Kuwahara-Arai, K., Ogawa, M., Shimojima, M., & Kirikae, T. (2020). Emergence of Carbapenem-Resistant Providencia rettgeri and Providencia stuartii Producing IMP-Type Metallo-β-Lactamase in Japan. Antimicrobial agents and chemotherapy, 64(11), e00382-20. doi:10.1128/AAC.00382-20

Jaÿ, M., & Tardy, F. (2019). Contagious Agalactia In Sheep And Goats: Current Perspectives. Veterinary medicine (Auckland, N.Z.), 10, 229–247. doi:10.2147/VMRR.S201847

Juneja, P., & Lazzaro, B. P. (2009). Providencia sneebia sp. nov. and Providencia burhodogranariea sp. nov., isolated from wild Drosophila melanogaster. International journal of systematic and evolutionary microbiology, 59(Pt 5), 1108–1111. doi:10.1099/ijs.0.000117-0

Kalogianni, A.I., Bossis, I., Ekateriniadou, L.V., & Gelasakis, A.I. (2020). Etiology, Epizootiology and Control of Maedi-Visna in Dairy Sheep: A Review. Animals : an open access journal from MDPI, 10(4), 616. doi:10.3390/ani10040616

Krake, P.R., & Tandon, N. (2004). Infective endocarditis due to Providenca stuartii. Southern medical journal, 97(10), 1022–1023. doi:10.1097/01.smj.0000141308.19657.ba

Ksentini, I., Gharsallah, H., Sahnoun, M., Schuster, C., Hamli Amri, S., Gargouri, R., Triki, M.A. Ksantini, M., & Leclerque, A. (2019). Providencia entomophila sp. nov., a new bacterial species associated with major olive pests in Tunisia. PLoS ONE, 14 (10); e0223943. doi:10.1371/journal.pone.0223943.

Lane D.J. (1992). 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques in bacterial systematics. New York: John Wiley & Sons. 115-175.

Liakopoulos, A., Oikonomou, O., & Wareham, D.W. (2017). Draft genome sequence of Providencia stuartii PS71, a multidrug-resistant strain associated with nosocomial infections in Greece. Genome Announc, 5(12): e00056-17. doi:10.1128/genomeA.00056-17.

Lin, K., Lin, A.N., Linn, S., Reddy, M., & Bakshi, A. (2017). Recurrent primary suprahepatic abscess due to Providencia Stuartii: a rare phenomenon. Cureus, 9(9), e1691. doi:10.7759/cureus.1691.

Liu, D. X., Didier, P. J., Plauche, G., & Pahar, B. (2016). Septicemia in an Indian Rhesus Macaque (Macaca mulatta) associated with Providencia stuartii. Journal of medical primatology, 45(6), 330–332. doi:10.1111/jmp.12230

Liu, J., Wang, R., & Fang, M. (2020). Características clínicas e de resistência aos medicamentos das infecções por Providencia stuartii em 76 pacientes. The Journal of international medical research, 48 (10), 300060520962296. doi:10.1177/0300060520962296

Muller, H.E., O’Hara, C.M., Fanning, G.R., Hickman-Brenner, F.W., Swenson, J.M., & Brenner, D.J. (1986). Providencia heimbachae, a new species of Enterobacteriaceae isolated from animals. International Journal of Systematic and Evolutionary Bacteriology, 36, 252-256. doi:10.1099/00207713-36-2-252.

O'Hara, C.M., Brenner, F.W., & Miller, J.M. (2000). Classification, identification, and clinical significance of Proteus, Providencia and Morganella. Clinical Microbiology Reviews, 13(4), 534-546. doi:10.1128/cmr.13.4.534546.2000.

Ovchinnikova, O. G., Rozalski, A., Liu, B., & Knirel, Y. A. (2013). O-antigens of bacteria of the genus Providencia: structure, serology, genetics, and biosynthesis. Biochemistry. Biokhimiia, 78(7), 798–817. doi:10.1134/S0006297913070110

Papadogiannakis, E., Perimeni, D., Velonakis, E., Kontos, V., & Vatopoulos, A. (2007). Providencia stuartii infection in a dog with severe skin ulceration and cellulitis. The Journal of small animal practice, 48(6), 343–345. doi:10.1111/j.1748-5827.2006.00266.x

Quinn, P. J., Markey, B. K., Leonard, F.C., Hartigan, P., Fanning, S., & Fitzpatrick E. S. (2011). Veterinary Microbiology and Microbial Disease. 2nd ed. Blackwell Science Ltd, Oxford. 1231p.

Sambrook, J., & Russell D. W. (2004). Molecular Cloning: A Laboratory Manual 3rd ed. Cold Spring Harbor Laboratory Press, New York, p.565 – 567.

Scott, P.R. (2011). Treatment and control of respiratory disease in sheep. The Veterinary clinics of North America. Food animal practice, 27(1), 175–186. doi:10.1016/j.cvfa.2010.10.016

Sipahi, O. R., Bardak-Ozcem, S., Ozgiray, E., Aydemir, S., Yurtseven, T., Yamazhan, T., Tasbakan, M., & Ulusoy, S. (2010). Meningitis due to Providencia stuartii. Journal of clinical microbiology, 48(12), 4667–4668. doi:10.1128/JCM.01349-10

Somvanshi, V. S., Lang, E., Sträubler, B., Spröer, C., Schumann, P., Ganguly, S., Saxena, A. K., & Stackebrandt, E. (2006). Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum. International journal of systematic and evolutionary microbiology, 56(Pt 3), 629–633. doi:10.1099/ijs.0.63973-0

Tavares, C. P., Pereira, P. S., Marques, E., Faria, C., Jr, de Souza, M., de Almeida, R., Alves, C., Asensi, M. D., & Carvalho-Assef, A. P. (2015). Molecular epidemiology of KPC-2-producing Enterobacteriaceae (non-Klebsiella pneumoniae) isolated from Brazil. Diagnostic microbiology and infectious disease, 82(4), 326–330. doi:10.1016/j.diagmicrobio.2015.04.002

Turner, S., Pryer, K. M., Miao, V. P., & Palmer, J. D. (1999). Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. The Journal of eukaryotic microbiology, 46(4), 327–338. doi:10.1111/j.1550-7408.1999.tb04612.x

Wie S. H. (2015). Clinical significance of Providencia bacteremia or bacteriuria. The Korean journal of internal medicine, 30(2), 167–169. doi:10.3904/kjim.2015.30.2.167

Downloads

Published

08/12/2021

How to Cite

KAGUEYAMA, F. C.; MARUYAMA, F. H.; COLODEL, E. M. .; PAVELEGINI, L. A. D.; NAKAZATO, L.; DUTRA, V. Bronchopneumonia in sheep associated with Providencia stuartii . Research, Society and Development, [S. l.], v. 10, n. 16, p. e117101623295, 2021. DOI: 10.33448/rsd-v10i16.23295. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/23295. Acesso em: 13 nov. 2024.

Issue

Section

Agrarian and Biological Sciences