Comparative study of different methods of extracting bioactive compounds from wild edible plants (WEP)
DOI:
https://doi.org/10.33448/rsd-v10i17.24210Keywords:
Antioxidants;; Phenolic compounds; Phytochemicals; WEP.Abstract
Wild Edible Plants (WEP) are rustic species, cosmopolitan and normally easily adaptable to edaphoclimatic. They have one or more edible parts and are not normally included in our everyday diet. Most of these species can have different biological activities, mainly as antioxidants. The objective of this work was to evaluate the antioxidant capacity and quantify the main phenolic compounds of Leandra regnellii Cogn. (pixirica), Solanum americanum Mill (maria-pretinha) and Phytolaca thyrsiflora Hill. (fitolaca), in three different extraction methods, namely: 1) aqueous by reflux, 2) aqueous by maceration and 3) hydroethanolic. The highest antioxidant activity obtained was for L. regnellii, under hydroethanolic extract and under reflux. As for the content of phenolic compounds, the best results were obtained for the species L. regnellii in maceration extract, followed by P. thyrsiflora in extract using a reflux system. As for the quantification of major compounds by HPLC, the species L. regnellii also presented the highest content of phenolic compounds in the hydroethanolic extract, especially ferulic acid, gallic acid, naringin, epicatechin and hesperidin. In the species P. thyrsiflora, gallic acid and quercetin were identified in hydroethanolic extract and apigenin in maceration extract. S. americanum in the hydroethanolic extract, presented rutin, catechin, epicatechin and vitexin as main components. The present study shows expressive antioxidant activity as well as the presence of phenolic compounds that have biological activity, highlighting the importance of choosing the extraction method for future uses of these species by the food and pharmaceutical industry.
References
Ahmed, S., Khan, H., Aschner, M., Hasan, M.M. & Hassan, S.T.S. (2019) Therapeutic potential of naringin in neurological disorders. Food Chemical Toxicoly. 132:110646.
Araújo, J. S., Chambó, E. D., Costa, M., Cavalcante da Silva, S., Lopes de Carvalho, C. A., & Estevinho, L. (2017). Chemical Composition and Biological Activities of Mono- and Heterofloral Bee Pollen of Different Geographical Origins. International Journal Of Molecular Sciences, 18(5), 921.
Baldermann, S., Blagojević, L., Frede, K., Klopsch, R., Neugart, S., Neumann, A., Ngwene, B., Norkeweit, J., Schröter, D., Schröter, A., Schweigert, F.J., Wiesner, M. & Schreiner, M. (2016) Are Neglected Plants the Food for the Future? Critical Reviews in Plant Sciences, 35:2, 106-119.
Barbosa, T. P., Lins, J. A. S., Silva, G. M. da, Valente, E. C. N., & Lima, A. S. T. (2021). Non-conventional food plants: are there potential consumers and places to buy them?. Research, Society and Development, 10(4), e27710414146.
BenSaad L. A., Kim K. H., Quah C. C., Kim W. R. & Shahimi M. (2017) Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum. BMC Complementary Alternative Medicin, 14;17(1):47.
Boari, F., Cefola, M., Di Gioia, F., Pace, B., Serio, F. & Cantore, V. (2013). Effect of cooking methods on antioxidant activity and nitrate content of selected wild Mediterranean plants. International Journal Of Food Sciences And Nutrition, 64(7), 870–876.
Corrêa, J., Bianchin, M., Lopes, A. P., Silva, E., Ames, F. Q., Pomini, A. M., Carpes, S. T., de Carvalho Rinaldi, J., Cabral Melo, R., Kioshima, E. S., Bersani-Amado, C. A., Pilau, E. J., de Carvalho, J. E., Ruiz, A., Visentainer, J. V., & Santin, S. (2021). Chemical profile, antioxidant and anti-inflammatory properties of Miconia albicans (Sw.) Triana (Melastomataceae) fruits extract. Journal Of Ethnopharmacology, 273, 113979.
Cunha, M. A. da, Pinto, L. C., Santos, I. R. P. dos, Neves, B. M., & Cardoso, R. de C. V. (2021). Neglected and Underutilized Species in the perspective of Food and Nutritional Security promotion in Brazil. Research, Society and Development, 10(3), e20610313306.
Chaudhary A., Jaswal V. S., Choudhary S., Sonika S. A., Beniwal V., Tuli, H. S. & Sharma S. (2019) Ferulic Acid: A Promising Therapeutic Phytochemical and Recent Patents Advances. Recent Patents on Inflammation & Allergy Drug Discovery, 13(2):115-123.
Cheptou, P.-O., Carrue, O., Rouifed, S., & Cantarel, A. (2008). Rapid evolution of seed dispersal in an urban environment in the weed Crepis sancta. Proceedings of the National Academy of Sciences, 105: 3796-3799.
Cho, K.W., Kim, Y.O., Andrade, J.E., Burgess, J.R. & Kim, Y.-C. (2011). Dietary naringenin increases hepatic peroxisome proliferators–activated receptor α protein expression and decreases plasma triglyceride and adiposity in rats. European Journal of Nutrition, 50, 81–88.
Dai J. & Mumper R. J. (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10):7313-7352.
D'Andrea G. (2015) Quercetin: A flavonol with multifaceted therapeutic applications. Fitoterapia. 106, 256–271.
D'Archivio M., Filesi C., Di Benedetto R., Gargiulo R., Giovannini C. & Masella R. (2007) Polifenóis, fontes dietéticas e biodisponibilidade. Annali dell'Istituto Superiore di Sanità, 43:348–361.
Del Tredici, P. (2010). Wild urban plants of the Northeast. Cornell University Press, Ithaca, New York, New York, USA. Book.
Di Lorenzo, C., Colombo, F., Biella, S., Stockley, C., & Restani, P. (2021). Polyphenols and Human Health: The Role of Bioavailability. Nutrients, 13(1), 273.
Du L, Wang J. X, Chen C. D., Wang F., Zhou H. Y. & Du W. (2018) Triterpenoid saponins from roots of Phytolacca acinosa. Zhongguo Zhong Yao Za Zhi. 43(12):2552-2556.
Ferreira-Gomes, L., Nascimento-Martins, D. H., Mazutti, S. M., de Barros, Y. Y., de Souza, P. M., Freitas, M. M., Fagg, C. W., Simeoni, L. A., Magalhães, P. O., Silveira, D., & Fonseca-Bazzo, Y. M. (2021). Propriedades biológicas y caracterización fitoquímica del extracto acuoso de Miconia chamissois Naudin. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, v. 20, n. 4, p. 427-442, 2021.
Fereidoon Shahidi, P. K. Janitha & P. D. Wanasundara (1992) Phenolic antioxidants, Critical Reviews in Food Science and Nutrition, 32:1, 67-103.
Filippin, L. I., Vercelino R., Marroni N. P. & Xavier R. M. (2008) Influência de processos redox na resposta inflamatória da artrite reumatóide. Revista Brasileira de Reumatologia, 48(1)17-24.
Ghorbani A. (2017) Mechanisms of antidiabetic effects of flavonoid rutin. Biomedicine & Pharmacotherapy, 96:305-312.
Habtemariam S. & Lentini G. (2015) The therapeutic potential of rutin for diabetes: an update. Mini-Reviews in Medicinal Chemistry, 15(7):524-8.
Habtemariam S. (2016) Rutin as a Natural Therapy for Alzheimer's Disease: Insights into its Mechanisms of Action. Current Medicinal Chemistry, 23(9):860-73.
Hameed, A., & Akhtar, N. (2018). Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries. Acta Pharmaceutica (Zagreb, Croatia), 68(1), 47–60.
Jiang J, Dai J, & Cui H. (2018) Vitexin reverses the autophagy dysfunction to attenuate MCAO-induced cerebral ischemic stroke via mTOR/Ulk1 pathway. Biomedicine & Pharmacotherapy, 99:583-590.
Khan, H. J., Ahmad, M. K., Khan, A. R., Rastogi, N., Mahdi, A. A., Ansari, J. A., Fatima, N., & Satyanarayan, G. (2016). Identification of Anticancer and Antioxidant phytoconstituents from chloroform fraction of Solanum nigrum L. berries using GC-MS/MS analysis. Indian Journal Of Experimental Biology, 54(11), 774–782.
Kaunda, J. S., & Zhang, Y. J. (2019). The Genus Solanum: An Ethnopharmacological, Phytochemical and Biological Properties Review. Natural Products And Bioprospecting, 9(2), 77–137.
Kinupp V. F. & Barros I.B.I.D. (2007) Riqueza de plantas alimentícias não-convencionais na região metropolitana de Porto Alegre, Rio Grande do Sul. Revista Brasileira de Biociências 5: 63-65.
Leal M. L, Alves R.P. & Hanazaki N. (2018) Knowledge, use, and disuse of unconventional food plants. Journal of Ethnobiology and Ethnomedicine, 14: 6.
Li Y, Yao J, Han C. (2016) Quercetin, Inflammation and Immunity. Nutrients, 8(3):167.
Ma X. P., Zhang W.F., Yi P., Lan J.J., Xia B., Jiang S., Lou H.Y. & Pan W.D. (2017) Novel Flavones from the Root of Phytolacca acinosa Roxb. Chemistry & Biodiversity, 14(12).
Maphosa Y. & Jideani V.A. (2017) The role of legumes in human nutrition. In: Hueda MC (eds) Functional Food - Improve Healththrough Adequate Food, 10.5772.
Meng, X., Li, Y., Lu, C., Zhao, M., Li, M., Wang, S., Zhao, C., Lin, B., Shang, L., Chu, Z., & Ding, X. (2020). Purification and antioxidant capacity analysis of anthocyanin glucoside cinnamic ester isomers from Solanum nigrum fruits. Journal Of Separation Science, 43(12), 2311–2320.
Morais, M. G., Saldanha, A. A., Rodrigues, J. P. C., Mendes, I. C., Ferreira, L. M., Amado, P. A., Farias, K. S., Zanuncio, V. S. S., da Silva, D. B., Pinto, F. C. H., Soares, A. C., & Lima, L. A. R. S. (2020). Chemical composition, antioxidant, anti-inflammatory and antinociceptive activities of the ethanol extract of ripe fruits of Solanum lycocarpum St. Hil. (Solanaceae). Journal Of Ethnopharmacology, 262, 113125.
Morelli, L.L.L., (2010), Avaliação de compostos fenólicos majoritários em geleia de uva produzida com a variedade IAC-138-22 (máximo). Dissertação de Mestrado. Universidade Estadual de Campinas. Campinas, SP, 133p.
Mu M., Zuo S., Wu R. M., Deng K. S., Lu S., Zhu J. J., Zou G. L., Yang J., Cheng M. L. & Zhao X. K. (2018) Ferulic acid attenuates liver fibrosis and hepatic stellate cell activation via inhibition of TGF-β/Smad signaling pathway. Drug Design, Development and Therapy, 3;12:4107-4115.
Nabavi S. M., Ebrahimzadeh M. A., Nabavi S. F., & Bahramian F. (2009) In vitro antioxidant activity of Phytolacca americana berries. Pharmacology, 1:81–8.
Nascimento V.T., Lucena R.F. Albuquerque U. P. & Maciel M.I. (2013) Knowledge and use of wild food plants in areas of dry seasonal forests in Brazil. Ecology of Food and Nutrition, 52: 317-43.
Neergheen V.S., Soobrattee M.A., Bahorun T. & Aruoma O.I. (2006) Caracterização dos constituintes fenólicos em plantas endêmicas da Ilha Maurícia como determinantes de suas atividades antioxidantes in vitro. Journal of Plant Physiology, 163:787–799.
Ola M.S., Ahmed M.M., Ahmad R., Abuohashish H.M., Al-Rejaie S.S. & Alhomida A.S. (2015) Neuroprotective Effects of Rutin in Streptozotocin-Induced Diabetic Rat Retina. Journal of Molecular Neuroscience, 56(2):440-8.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa cientifica. [e-book]. Santa Maria: UAB / NTE / UFSM. https://www.ufsm.br/app/uploads/sites/358/2019/02/Metodologia-da-Pesquisa-Cientifica_final.pdf.
Petropoulos, S.A., Karkanis, A., Martins, N., & Ferreira, I.C.F.R. (2018) Edible halophytes of the Mediterranean basin: Potential candidates for novel food products. Trends in Food Science and Technology, 74, 69–84.
Prakash, D., & Sharma, G. (2014) Role of antioxidant polyphenols in nutraceuticals and human health. In: Prakash D, Sharma G (ed) Phytochemicals Of Nutraceutical Importance. CABI, Wallingford, UK, pp 208–228.
Quintans-Júnior, L. J., Gandhi, S. R., Passos, F., Heimfarth, L., Pereira, E., Monteiro, B. S., Dos Santos, K. S., Duarte, M. C., Abreu, L. S., Nascimento, Y. M., Tavares, J. F., Silva, M. S., Menezes, I., Coutinho, H., Lima, Á., Zengin, G., & Quintans, J. (2020). Dereplication and quantification of the ethanol extract of Miconia albicans (Melastomaceae) by HPLC-DAD-ESI-/MS/MS, and assessment of its anti-hyperalgesic and anti-inflammatory profiles in a mice arthritis-like model: Evidence for involvement of TNF-α, IL-1β and IL-6. Journal Of Ethnopharmacology, 258, 112938.
Ribani M., Bottoli C.B.G., Collins C.H., Jardim I.C.S.F. & Melo L.F.C. (2004) Validação em métodos cromatográficos e eletroforéticos. Química Nova, 27, 771-780.
Saija A, Tomaino A, Trombetta D, De Pasquale A, Uccella N, Barbuzzi T, Paolino D. & Bonina F. (2000) In vitro and in vivo evaluation of caffeic and ferulic acids as topical photoprotective agents. International Journal of Pharmaceutics, 10;199(1):39-47.
Santos-Buelga, C., González-Paramás, A. M., Oludemi, T., Ayuda-Durán, B., & González-Manzano, S. (2019). Plant phenolics as functional food ingredients. Advances In Food And Nutrition Research, 90, 183–257.
Tufarelli, V., Casalino, E., D'Alessandro, A. G., & Laudadio, V. (2017). Dietary Phenolic Compounds: Biochemistry, Metabolism and Significance in Animal and Human Health. Current Drug Metabolism, 18(10), 905–913.
Ullah, N., Haq, I. U., Safdar, N., & Mirza, B. (2015). Physiological and biochemical mechanisms of allelopathy mediated by the allelochemical extracts of Phytolacca latbenia (Moq.) H. Walter. Toxicology And Industrial Health, 31(10), 931–937.
Venturini C.L., Macho A., Arunachalam K., de Almeida D.A.T, Rosa S.I.G., Pavan E., Balogun S.O., Damazo A.S. & Martins D.T.O. (2018) Vitexin inhibits inflammation in murine ovalbumin-induced allergic asthma. Biomedicine & Pharmacotherapy, 97:143-151.
Xianchu L, Lan Z, Ming L, Yanzhi M. (2018) Protective effects of rutin on lipopolysaccharide-induced heart injury in mice. The Journal of Toxicological Sciences, 43(5):329-337.
Xiang, L., Wang, Y., Yi, X., & He, X. (2019). Steroidal alkaloid glycosides and phenolics from the immature fruits of Solanum nigrum. Fitoterapia, 137, 104268.
Xu B.J., Chang S.K. (2007) A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. Journal of Food Science, 72:S159–166.
Yang, L., Gao, S., Su, Z., Qin, X., & Li, Z. (2021). Identification of the constituents and the cancer-related targets of the fruit of Solanum nigrum based on molecular docking and network pharmacology. Journal Of Pharmaceutical And Biomedical Analysis, 200, 114067.
You, H. L., Huang, C. C., Chen, C. J., Chang, C. C., Liao, P. L. & Huang, S. T. (2018) Anti-pandemic influenza A (H1N1) virus potential of catechin and gallic acid. Journal of the Chinese Medical Association, 81(5):458-468.
Zduńska, K., Dana, A., Kolodziejczak, A., & Rotsztejn, H. (2018) Antioxidant Properties of Ferulic Acid and Its Possible Application. Skin Pharmacology and Physiology, 31(6):332-336.
Zheleva-Dimitrova D. (2013). Antioxidant and acetylcholinesterase inhibition properties of Amorpha fruticosa L. and Phytolacca americana L. Pharmacognosy Magazine, 9(34), 109–113.
Zhou, C., Lai, Y., Huang, P., Xie, L., Lin, H., Zhou, Z., Mo, C., Deng, G., Yan, W., Gao, Z., Huang, S., Chen, Y., Sun, X., Lv, Z., & Gao L. (2019) Naringin attenuates alcoholic liver injury by reducing lipid accumulation and oxidative stress. Life Sciences, 1;216:305-312.
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Copyright (c) 2021 Luana Minello; Valdirene Camatti Sartori; Luciana Bavaresco Andrade Touguinha; Fabiana Agostini; Sidnei Moura e Silva; Mirian Salvador
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