Monitoring the particle size distribution of whole and skimmed milk during acidic or enzymatic coagulation process
DOI:
https://doi.org/10.33448/rsd-v11i1.24438Keywords:
Particle size; Microstructure; Milk-clotting; Milk protein.Abstract
The milk coagulation process can occur mainly in two ways: acidic or enzymatic coagulation, the characteristic of the gel obtained being dependent on the type of coagulation, the type of processing applied and the composition of the initial milk. Therefore, the objective of this work was to evaluate the microstructural modifications of pasteurized whole and skimmed milk through particle distribution analysis (LS), through acidic coagulation, carried out via fermentation by adding a mixed culture of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus; and enzymatic, by the addition of microbial chymosin. During the entire coagulation process, milk aliquots were removed from both coagulations until gels were obtained. The gels obtained showed differences in coagulation time and particle size distribution, in addition, the particles sizes of the skimmed milks gels showed final values in all distributions greater than that of whole milk, possibly related to the fact of casein, protein majority, be more available in the reaction medium so that the destabilization of the system occurs in both types of coagulation. When comparing the two coagulation processes for the d90 distribution, the enzymatic coagulation gels showed aggregates 12 times larger than the acid coagulation gels, demonstrating that the interaction forces for the formation of aggregates are much greater in enzymatic coagulation.
References
Arango, O., Trujillo, A. J. & Castillo, M. (2012). Influence of fat replacement by inulin on rheological properties, kinetics of rennet milk coagulation, and syneresis of milk gels. Journal of Dairy Science, 96, 1-13.
Banon, S. & Hardy, J. (1992). A colloidal aprroach of milk acidification by glucono-delta-lactone. Journal of Dairy Science, 75, 935-941.
Bauland, J., Famelart, M. H., Bouhallab, S., Jeantet, R., Roustel, S., Faiveley, M. & Croguennec, T. (2020). Addition of calcium and magnesium chlorides as simple means of varying bound and precipitated minerals in casein micelle: Effect on enzymatic coagulation. Journal of Dairy Science, 103, 9923-9935.
Brar, S. K. & Verma, M. (2011). Measurement of nanoparticles by light-scattering techniques. TrAC Trends in Analytical Chemistry, 30 (1), 4-17.
Brulé, G. & Maubois, J.-L. (2018). Coagulation du lait. In: Gillis, J. C. & Ayerbe, A. Le fromage. (4a ed.), Lavoisier, 116-136.
Castillo, M. Z., Payne, F. A., Hicks, C. L., Laencina, J. S. & López, M. B. M. (2003). Modelling casein aggregation and curd firming in goats’ milk from backscatter of infrared light. Journal of Dairy Research, 70, 335-348.
de Kruif, C.G. (1999). Casein micelle interactions. International Dairu Journal, 9, 183-188.
Dwyer, C., Donnelly, L. & Buckin, V. (2005). Ultrasonic analysis of rennet-induced pre-gelation and gelation processes in milk. Journal of Dairy Research, 72, 303-310.
Freitas, C. D. T., Silva, M. Z. R., Oliveira, J. P. B., Silva, A. F. B., Ramos, M. V. & Sousa, J. S. (2019). Study of milk coagulation induced by chymosin using atomic force microscopy. Food Bioscience, 29, 81-85.
Leite Júnior, B. R. C., Tribst, A. A. & Cristianini, M. (2017). Comparative study among rheological, near-infrared light backscattering and confocal microscopy methodologies in enzymatic milk coagulation: Impact of different enzyme and protein concentrations. Food Hydrocolloids, 62, 73-82.
Lucey, J.A., Wilbanks, D.J. & Horne, D.S. (2022). Impact of heat treatment of milk on acid gelation. International Dairy Journal, 125, 105222.
Lucey, J. A. (2011). Rennet-Induced coagulation of milk. Encyclopedia of Dairy Sciences, 579-584.
Lucey, J. A. (2002). Formation and physical properties of milk protein gels. Journal of Dairy Science, 85 (2), 281-294.
Lyndgaard, C. B., Engelsen, S. B. & van den Berg, F. W. J. (2012). Real-time modeling of milk coagulation using in-line near infrared spectroscopy. Journal of Food Engineering, 108, 345-352.
McMahon, D. J. & Brown, R. J. (1984). Enzymic coagulation of casein micelles: A review. Journal of Dairy Science, 67, 919-929.
Mimouni, A., Deeth, H. C., Whittaker, A. K., Gidley, M. J., Bhandari, B. R. (2009). Rehydration process of milk protein concentrate powder monitored by static light scattering. Food Hydrocolloids, 23 (7), 1958-1965.
Nicolau, N., Buffa, M., O’Callaghan, D. J., Guamis, B. & Castillo, M. (2015). Estimation of clotting and cutting times in sheep cheese manufacture using NIR light backscatter. Dairy Science & Technology, 95, 495-507.
Panikuttira, B., Payne, F. A., O’Shea, N., Tobin, J. T., O’Callaghan, D. J. & O’Donnell, C. P. (2019). Investigation of an in-line protorype fluorescence and infrared backscatter sensor to monitor rennet-induced coagulation of skim milk at different protein concentrations. International Journal of Food Science and Technology, 1-8.
Roefs, S. P. F. M., Walstra, P., Dalgleish, D. G. & Horne, D. S. (1985). Preliminary note on the change in casein micelles caused by acidification. Netherlands Milk Dairy, 39, 119-122.
Silva, N. N., Casanova, F., Pinto, M. S., Carvalho, A. F. & Gaucheron, F. (2019). Casein micelles: from the monomers to the supramolecular structure. Brazilian Journal of Food Technology, 22, e2018185.
Sørensen, I., Le, T. T., Larsen, L. B. & Wiking, L. (2019). Rennet coagulation and calcium distribution of raw milk reverse osmosis retentate. International Dairy Journal, 95, 71-77.
Tarapata, J., Lobacz, A. & Zulewska, J. (2021). Physicochemical properties of skim milk gels obtained by combined bacterial fermentation and renneting: Effect of incubation temperature at constant inoculum level. International Dairy Journal, 123, 105167.
Torres, J. K. F., Stephani. R., Tavares, G. M., Carvalho, A. F., Costa, R. G. B., Almeida, C. E. R., Almeida, M. R., Oliveira, L. F. C., Schuck, P. & Perrone, I. T. (2017). Technological aspects of lactose-hydrolyzed milk powder. Food Research International, 101, 45-53.
Tuinier, R. & de Kruif, C. G. (2002). Stability of casein micelles in milk. The Journal of Chemical Physics, 117, 1290.
van Hooydonk, A. C. M., Hagedoorn, H. G. & Boerrigter, I. J. (1986). The effect of various cations on the reeneting of milk. Netherlands Milk and Dairy Journal, 40, 369-390.
Wasltra, P. (1990). On the stability of casein micelles. Journal of Dairy Science, 73, 1965-1979.
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Copyright (c) 2022 Fernanda Lopes da Silva; Mariana Braga de Oliveira; Érica Felipe Mauricio; Elisângela Ramieres Gomes; Ítalo Tuler Perrone; Antônio Fernandes de Carvalho; Rodrigo Stephani
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