Pathogenicity of Beauveria bassiana strains against Drosophila suzukii (Diptera: Drosophilidae)

Authors

DOI:

https://doi.org/10.33448/rsd-v11i2.25730

Keywords:

Spotted-wing drosophila; Microbial control; Entomopathogenic fungi.

Abstract

The biological control (BC) is an efficient tool in helping to reduce pests in crops of economic interest. In addition to the efficiency, there are advantages in using biological control agents, such as human safety and other non-target organisms and decreasing pesticide residues. The general aim of this work was to evaluate in vitro the pathogenicity power of fifteen Beauveria bassiana strains aiming the biological control of Drosophila suzukii (Matsumura, 1931). Successful infection of entomopathogenic fungi depends primarily on the adherence and penetration ability of a fungus to the host integuments. In addition, scanning electron microscopy was realized. To verify the spore adherence after the spraying tests, insects were collected throughout the bioassays in order to confirm spore evolution on the insect cuticle. In this work, D. suzukii adults were not susceptible to the aqueous suspensions of conidia at concentrations of 108 spores·mL−1 of these strains. There wasn’t death confirmed by fungus extrusion after maintenance in humid chamber.

Author Biographies

Jason Lee Furuie, Federal University of Paraná

Post-Graduate Program in Microbiology, Parasitology and Pathology

Andressa Katiski da Costa Stuart, Federal University of Paraná

Post-graduat Program in Microbiology, Parasitology and Pathology

Franciele Baja, Federal University of Paraná

Post-Graduate Program in Microbiology, Parasitology and Pathology

Morgana Ferreira Voidaleski, Federal University of Paraná

Post-Graduate Program in Microbiology, Parasitology and Pathology

Maria Aparecida Cassilha Zawadneak, Federal University of Paraná

Post-Graduate Program in Agronomy - Vegetal Production

Ida Chapaval Pimentel, Federal University of Paraná

Post-Graduate Program in Microbiology, Parasitology and Pathology

References

Akbar, W., Lord, J. C., Nechols, J. R., Howard, R. W. (2004). Diatomaceous earth Increases the efficacy of Beauveria bassiana against Tribolium castaneum larvae and increases conidia attachment. J Econ Entomol, 97(2), 273-80.

Al-aidoors, K., Roberts, D. R. (1978). Mutants of Metarhizium anisopliae with increased virulence toward mosquito larvae. Genet. Cytol., 11, 211-219.

Alvin, A., Miller, K. I., Neilan, B. A. (2014). Exploring the potential of endophytes from medical plants as sources of antimycobacterial compounds. Microbiol Research, 169(7), 486-95.

Amatuzzi, R. F., et al. (2018). Fungos endofíticos para o biocontrole de Duponchelia fovealis (Zeller) (Lepidoptera:Crambidae), Braz J Biol, 78(3), 429-435.

Alves, S. B., Pereira, R. M. (1998). Produção de fungos entomopatogênicos. In Controle microbiano de insetos, Alves, S.B. 2.ed. (Piracicaba: Fealq.), pp. 845-869.

Amil-ruiz, F., Blanco-portales, R., Muñoz-blanco, J., Caballero, J.L. (2011). The Strawberry plant defense mechanism: a molecular review. Plant and cell phisiology, 52(11), 1873-1903.

Bernardi, D., Botton, M., Nava, D. E., Zawadneak, M. A. C. (2015). Guia para identificação e monitoramento de pragas e seus inimigos naturais em morangueiro. (Brasília: Embrapa), pp. 23-29.

Buchón, N., Silverman, N., Cherry, S. (2014). Immunity in Drosophila melanogaster from microbial recognition to whole-organism physiology. Nature reviews: immunology, 14, 796-809.

Butt, T. M., Jackson, C., Magan, N. (2001). Introduction - fungal biological control agents: progress, problems, and potential. In fungi as biocontrol agents: progress, problems and potential. Cabi publishing (New York, USA, p.1-8).

Calabria, G., Maca, J., Bachli, G., Serra, L., Pascual, M. (2012). First records of the Potential pest species Drosophila suzukii (diptera: drosophilidae) in Europe. J appl entomol, 136, 139–147.

Coelho Junior, J. M. (2016). Strawberry cultivars: knowing to expand and reduce the environmental impacts. Geama, 2(2).

Corassa, J. N., Machiner, M., Valladao, D. M. S., Andrighetti, C. R., Weberling, J. B. (2002). Efeito de extrato etanólico de plantas sobre Spodopotera frugiperda (Lepdoptera: Noctuidae). Research, Society and Development, 11(2).

Deprá M., Poppe J. L., Schmitz H. J., De Toni D. C., Valente V. L. S. (2014). The first records of the invasive pest Drosophila suzukii in the South American continent. J. Pest sci., 87, 379–383.

Emiljanowicz, L. M., Ryan, G. D., Langille, A., Newman, J. (2014) Development, reproductive output, and population growth of the fruit by pest Drosophila suzukii (diptera: drosophilidae) on artificial diet. Journal of economic entomology, 107(4), 1392- 1398.

Faria, M. R., Wraight, S. (2007). Mycoinsecticides and mycoacaricide: a comprehensive list with worldwide coverage and international classification of formulation types. Biological control, 43(3), 237-256.

Furuie, J. L., Stuart, A. K. C., Voidaleski, M. F., Zawadneak, M. A. C., Pimentel, I. C. (2022). Isolation of Beauveria strains and their potential as control agents for Lema bilineata Germar (Coleoptera: Chrysomelidae). Insects, 13,93.

Geisler, F. C. S., Santos, J., Holdefer, D. R., Garcia, F. R. M. (2015). Primeiro registro de Drosophila suzukii (Matsumura,1931) Diptera:drosophilidae para o estado do Paraná, Brasil e de novos hospedeiros. Revista de ciências ambientais, 9(2), 125-129.

Gutierrez-Palomares, V. M., Paulino-Alonso, L., Gutierrez, J. Z., Alatorre-Rosas, R. (2021). Pathogenicity and virulence of Isaria javanica, Metarhizium anisopliae, and Beauveria bassiana strains for control of Drosophila suzukii (Matsumura). Southwestern Entomologist, 46(4), 853-860.

Grupta, S., Dikshit, A. K. (2010). Biopesticides: an ecofriendly approach for pest control. J biopestic., 3, 186-188.

Hajek, A.E., Delalibera JR., I. (2010). Fungal pathogens as classical biological control agents against arthropods. Biocontrol., 55(1), 147-158.

Lee, J. C., Dreves, A. J., Cave, A. M., Kawai, S., Isaacs, R., Miller, J. C., Van Timmeren, S., Bruck, D. J. (2015). Infestation of wild and ornamental non crop fruits by Drosophila suzukii (Diptera: drosophilidae). Annals of the entomological society of America, 3, 1–13.

Myers, J. H., Cory, J.S. (2017). Biological control agents: invasive species or valuable solutions? Impact of biological invasions on ecosystem services., 191-202.

Ortiz-Urquiza, A., Keyhani, N.O. (2013). Action on the surface: entomopathogenic fungi versus the insect cuticle. Insects, 4(3), 357-374

Pell, J. K., Hannam, J. J., Steinkraus, J.J. (2010). Conservation biological control using fungal entomopathogens. Biological Control., 55, 187–198.

Pham, T. A., Kim, J. J., Kim, S. G., Kim, K. (2009). Production of blastospore of entomopathogenic Beauveria bassiana in a submerged batch culture. Mycobiology, 37(3), 218-224.

Poitevin, C. G., Porsani, M. V., Poltronieri, A.S., Zawadneak, M.A.C., Pimentel, I.C. (2018). Fungi isolated from insects in strawberry crops act as potential biological control agents of Duponchelia fovealis (Lepidoptera:Crambidae). Applied entomology and zoology.

Roberts, D. W., Ieger, R. S. (2004). Metharizium spp., cosmopolitan insect-pathogenic fungi: mycological aspects. Advances in applied microbiology., 54, 1-70.

Tragust, S., Mitteregger, B., Barone, V., Konrad, M., Ugelvig, L.V., Cremers, S. (2013). Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. Curr biol, 23(1), 76-82.

Tuininga, A. R., Miller, J.L., Morath, S.H., et al. (2009). isolation of entomopathogenic fungi from soils and ixodessacpularis (acari: ixodidae) ticks: prevalence and methods. J. Med. Entomol, 46(3), 557-567.

Van Lenteren, J. C., Bolckmans, K., Kohl, J., Ravensberg, W.J., Urbaneja, A. (2018). Biological control using invertebrates and microorganisms: plenty of new opportunitties. Biocontrol, 63, 39-59.

Xiao, G., Ying, S. H., Zheng, P., Wang, Z. L., Zhang, S., Xie, X. Q., Shang, Y.; ST Leger, R. J., Zhao, G. P., Wang, C., Feng, M.G. (2012). Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana, sci rep, 2, 483.

Woltz, J. M., Donahue, K. M., Bruck, D. J. Lee, J. C. (2015). Efficacy of commercially available predators, nematodes and fungal entomopathogens for augmentative control for Drosophila suzukii. Journal of Applied Entomology, 139(10), 759-770.

Downloads

Published

29/01/2022

How to Cite

FURUIE, J. L.; STUART, A. K. da C.; BAJA, F.; VOIDALESKI, M. F.; ZAWADNEAK, M. A. C.; PIMENTEL, I. C. Pathogenicity of Beauveria bassiana strains against Drosophila suzukii (Diptera: Drosophilidae). Research, Society and Development, [S. l.], v. 11, n. 2, p. e41611225730, 2022. DOI: 10.33448/rsd-v11i2.25730. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/25730. Acesso em: 16 nov. 2024.

Issue

Section

Agrarian and Biological Sciences