Toxicological evaluation and safety of the ethanol extract from leaves of Piptadenia stipulaceae
DOI:
https://doi.org/10.33448/rsd-v11i3.26815Keywords:
Jurema Branca; Osmotic fragility; In vitro toxicity.Abstract
Piptadenia stipulacea is popularly known as “Jurema Branca” and is apply in empiric therapy as anti-inflammatory, analgesic, cells regenerator, antipyretic and astringent pectoral. The present work had as objective to carry out the toxicological analysis of the ethanol extract of the leaves of P. stipulacea. In vitro toxicity was performed according to the methodologies of osmotic fragility against sheep erythrocytes and genotoxicity by Allium cepa and in vivo toxicity according to the protocol of Organization for Economic Cooperation and Development (OECD 423). Erythrocyte osmotic fragility presented low levels of hemolysis both by the qualitative evaluation of the supernatant and by the hemolytic percentage result. In cytotoxic evaluation with Allium cepa, no chromosomal abnormalities or differences in the cell division process (interphase, prophase, anaphase metaphase and telophase) were identified between the control group and the groups submitted to the different concentrations of the ethanolic extract of the leaves of P. stipulaceae (50μg/mL, 500μg/mL and 1000μg/mL). The ethanolic extract of the leaves of P. stipulaceae can be considered of low acute toxicity, since it did not cause the death of treated animals at the dose of 2000 mg/kg, as recommended by OECD 423. The results obtained allow to proceed with additional tests of sub-acute and chronic toxicity, biological activities on specific organisms in order to define the low risk of this plant.
References
Al-Asmari, A. K., Al-Elaiwi, A. M., Athar, M. T., Tariq, M., Al Eid, A., & Al-Asmary, S. M. (2014). A review of hepatoprotective plants used in Saudi traditional medicine. Evidence-Based Complementary and Alternative Medicine, 2014.
Bezerra, D. A. C., Rodrigues, F. F. G., da Costa, J. G. M., Pereira, A. V., de Sousa, E. O., & Rodrigues, O. G. (2011). Abordagem fitoquímica, composição bromatológica e atividade antibacteriana de Mimosa tenuiflora (Wild) Poiret e Piptadenia stipulacea (Benth) Ducke. Acta Scientiarum. Biological Sciences, 33(1), 99-106.
Da Silva, T. M. S., Sobreira, R. C. B., Silva, J. D. A. G., da Costa, M. A. S., de Assis Lima, M. I., da Silva, A. A., & Leite, S. P. (2019). Fragilidade osmótica eritrocitária e atividade citotóxica do extrato aquoso de Indigofera suffruticosa Mill (Fabaceae). Revista Virtual de Química, 11(6).
De Melo Cavalcanti-Dantas, V., de Medeiros, K. L., de Azevêdo, T. K. B., Santana, G. M., Pereira, A. V., Góis, M. B., & Pereira, J. V. (2016). Taninos: principal componente do extrato Piptadenia stipulacea (Benth) Ducke inibe o crescimento de cepas clínicas de Staphylococcus aureus de origem bovina. Biotemas , 29 (1), 109-114.
De Queiroz, A. C., de Lira, D. P., Dias, T. D. L. M. F., de Souza, É. T., da Matta, C. B. B., de Aquino, A. B., & Alexandre-Moreira, M. S. (2010). The antinociceptive and anti-inflammatory activities of Piptadenia stipulacea Benth.(Fabaceae). Journal of ethnopharmacology, 128(2), 377-383.
De Sousa, M. A. N., da Silva Filho, E. F., de Assis Nóbrega, N. J., & de Lima Costa, E. (2014). Ipomoea asarifolia e seus efeitos tóxicos e genotóxicos em animais de produção. Revista Saúde & Ciência Online, 3(3), 240-252.
Didelon, J., Mazeron, P., Muller, S., & Stoltz, J. F. (2000). Osmotic fragility of the erythrocyte membrane: characterization by modeling of the transmittance curve as a function of the NaCl concentration. Biorheology, 37(5, 6), 409-416.
Emanuelli, M. P., dos Anjos Lopes, S. T., Maciel, R. M., Garmatz, B. C., & de Oliveira Tavares, M. (2008). Concentração Sérica de Fosfatase Alcalina, Gama-glutamil transferase, uréia e creatinina em coelhos (Oryctolagus cuniculus). Ciência Animal Brasileira, 9(1), 251-255.
Fabricante, J. R. & Andrade, L. A. Análise estrutural de um remanescente de caatinga no seridó paraibano. O ecologia Brasiliensis, 11(3), 341-349, 2007.
Fechine, I. M., Alves, K. D. S. B., da Silveira Muniz, R. F., Soares, C. L. R., da Silva Alves, H., da Silva, T. G., & de Souza, E. M. L. (2020). Evaluation of the cytotoxicity of products obtained from Calotropis procera (Apocynaceae). Research, Society and Development, 9(12), e4391210723-e4391210723.
Fish, M. S.; Johnson, N. M.; Horning, E. C. Piptadenia alkaloids. Índole bases of Piptadenia peregrina and related species. Journal of the American Chemical Society, 77(22), 5892-5895, 1955
Florentino, A. T. N.; Araújo, E. L.; Albuquerque, U. P. Contribuição de quintais agroflorestais na conservação de plantas da caatinga, município de Caruaru, PE, Brasil. Acta Botânica Brasílica, 21(1), 37-47, 2007.
González-de-Peredo, A. V., Vázquez-Espinosa, M., Espada-Bellido, E., Carrera, C., Ferreiro-González, M., Barbero, G. F., & Palma, M. (2021). Flavonol composition and antioxidant activity of onions (Allium cepa L.) based on the development of new analytical ultrasound-assisted extraction methods. Antioxidants, 10(2), 273.
Guerra, M., & Souza, M. D. (2002). Como observar cromossomos: um guia de técnicas em citogenética vegetal, animal e humana. Ribeirão Preto: FUNPEC, 201.
Henry, J. B. (2008). Diagnósticos clínicos e tratamento por métodos laboratoriais. In Diagnósticos clínicos e tratamento por métodos laboratoriais (pp. 1734-1734).
Junqueira, L. C., & Carneiro, J. (2008). Histologia Básica. Texto e Atlas. (11a ed.). Guanabara.
Klein, L., Massie, B. M., Leimberger, J. D., O’Connor, C. M., Piña, I. L., Adams Jr, K. F., & Gheorghiade, M. (2008). Admission or changes in renal function during hospitalization for worsening heart failure predict postdischarge survival: results from the Outcomes of a Prospective Trial of Intravenous Milrinone for Exacerbations of Chronic Heart Failure (OPTIME-CHF). Circulation: Heart Failure, 1(1), 25-33.
Mincis, M., & Mincis, R. (2006). Enzimas hepáticas: aspectos de interesse prático. Revista Brasileira de Medicina, 56-60.
Oecd 2001. Guideline 423: Acute Oral Toxicity - Acute Toxic Class Method. http://www.oecd.org/publications.
Pereira, K. T. O., Paiva, E. P. D., Souza Neta, M. L. D., Benedito, C. P., & Torres, S. B. (2020). Avaliação da qualidade fisiológica de sementes de Piptadenia stipulacea (Benth.) Ducke pelo teste de tetrazólio. Revista Ciência Agronômica, 51.
Sobreira, R. C. B., de Assis Lima, M. I., dos Santos, E. R. S. L., da Silva, T. M. S., da Costa, M. A. S., da Alves, W. S., & Leite, S. P. (2020). Phytochemical Approach and Evaluation of the Osmotic Fragility and Cytotoxic Activity of Piptadenia stipulacea (Benth) Ducke. Brazilian Journal of Health Review, 3(6), 16917-16932.
Sturbelle, R. T., Pinho, D. S. D., Restani, R. G., Oliveira, G. R. D., Garcias, G. D. L., & Martino-Roth, M. D. G. (2010). Avaliação da atividade mutagênica e antimutagênica da Aloe vera em teste de Allium cepa e teste de micronúcleo em linfócitos humanos binucleados. Revista Brasileira de Farmacognosia, 20(3), 409-415.
Vaghasiya, Y. K., Shukla, V. J., & Chanda, S. V. (2011). Acute oral toxicity study of Pluchea arguta boiss extract in mice. J Pharmacol Toxicol, 6(2), 113-23.
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Copyright (c) 2022 Renatha Claudia Barros Sobreira; Willams Alves da Silva; Tainá Maria Santos da Silva; Marcos Aurélio Santos da Costa; Mary Anne Medeiros Bandeira; Thaís Emmanuelly Melo dos Santos; Maria Isabel de Assis Lima; Anderson Arnaldo da Silva; Sandrine Maria de Arruda Lima; Sônia Pereira Leite
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