Identification of altered microRNA target genes and pathways in cervical intraepithelial neoplasia
DOI:
https://doi.org/10.33448/rsd-v11i3.26864Keywords:
NIC; MicroRNAs; In silico; Pathways.Abstract
MicroRNAs are single-stranded, non-protein-coding RNA molecules of about 18-25 nucleotides, which are capable of regulating expression at the post-transcriptional level. Its dysregulation has been widely studied in cancer, but little has been documented in pre-malignant lesions, such as Cervical Intraepithelial Neoplasms (CIN). In this context, this study aims to search the literature for microRNAs associated with NICs, as well as perform in silico prediction, in order to know the main genes and pathways that are regulated by them. For this, a search was carried out in the literature regarding the association of microRNAs in pre-neoplastic lesions in cervical cancer. The research was carried out in databases such as Pubmed and Science Direct, and scientific articles with publication date between the years 2010 to 2021 were selected and analyzed. , through bioinformatics tools. According to the inclusion and non-inclusion selection criteria, 8 articles were included in the literature review, which totaled 22 deregulated microRNAs in cervical intraepithelial lesions, with 18 microRNAs in high-grade lesions and 4 microRNAs in low-grade lesions. In silico analysis revealed that these microRNAs regulate genes such as: FZD7, ESR1 and BRAF, which are involved in major carcinogenesis pathways, such as the proteoglycan and stem cell pathway. The data presented demonstrate the deregulation of these biomolecules in pre-malignant cervical cancer samples.
References
Acunzo, M., Romano, G., Wernicke, D., & Croce, C. M. (2015). MicroRNA and cancer--a brief overview. Advances in Biological Regulation - Journals - Elsevier, 57, 1-9. 10.1016/j.jbior.2014.09.013.
Alarm, S., Anugraham, M., Huang, Y., Kohler, R.S., Hettich, T., Winkelbach, K., Grether, Y., López, M. N., Khasbiulina, N., Bovin, N. Schlotterbeck, G., & Jacob, F. (2017). Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells. Scientific Reports, 30 (7), 45367.doi.10.1038/srep45367.
Albernaz, D. D. P. Modulação da Expressão e da Síntese de Actina por Citrato de Ródio II em Células de Câncer Mamário. Brasília. Dissertação- (Mestrado em Nanociência e Nanobiotecnologia) - Universidade de Brasília, Brasilia, 2019.
Aokes, S. O & Papa, F.R. (2015). The role of endoplasmic reticulum stress in human pathology. Annual Review of Pathology: Mechanisms of Disease, 10, 173-94. 10.1146/annurev-pathol-012513-104649.
Bai, X., Han, G., Liu, Y., Jiang, H., & He, Q. (2018). MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3. Biomedicine & Pharmacotherapy,103, 1482-1489. 10.1016/j.biopha.2018.04.165.
Bennerjee, S. & Zhang, W. (2018). Endoplasmic Reticulum: Target for Next-Generation Cancer Therapy. Chembiochem, 16(19), 2341-2343.10.1002/cbic.201800461.
Castle, F.E., Murokora, D., Perez, C., Alvarez, M., Quek, S. C., & ChampbelL, C. (2017). Treatment of cervical intraepithelial lesions. International Journal of Gynecology & Obstetrics, 138 (1), 20-25. 10.1002/ijgo.12191.
Cohen, P. A., Jhinggran, A., Oaknin, A., & Denny, L. (2019). Cervical cancer. Lanceta, 393 (10167), 169-182. 10.1016/S0140-6736(18)32470-X.
Colak, S. & Dijke, P.T. (2017). Targeting TGF-β Signaling in Cancer. Trends in Cancer: Cell Press, 3(1), 56-71.10.1016/j.trecan.2016.11.008
Fang, S., Guo, J., Zhang, J., Liu, J., Hong, S., Yu, B., Gao, Y., Hu, S., Liu, H., Sun, L., & Zhao, Y. (2020). A P53-related microRNA model for predicting the prognosis of hepatocellular carcinoma patients. Journal of Cellular Physiology, 235 (4), 3569-3578. 10.1002/jcp.29245.
Huang, J., Jiang, W., Tong, X., Zhang, LI., Zhang, Y., & Fan, H. (2019). Identification of gene and microRNA changes in response to smoking in human airway epithelium by bioinformatics analyses. Medicine (Baltimore), 98 (38), 17267. 10.1097/MD.0000000000017267.
Instituto Nacional de Câncer José Alencar Gomes da Silva. Estimativa 2020: incidência de câncer no Brasil / Instituto Nacional de Câncer José Alencar Gomes da Silva. – Rio de Janeiro: INCA, 2020.
Jiang, Y., Hu, Z., Zuo, Z., Li, Y., Pu, F., Wang, B., Tang, Y., Guo, Y., & Tao, H. (2020). Identification of Circulating MicroRNAs as a Promising Diagnostic Biomarker for Cervical Intraepithelial Neoplasia and Early Cancer: A Meta-Analysis. BioMed Research International, 2020, 1-14. 10.1155/2020/4947381.
Johnson, C. A., James, D., Marzan, A., & Armaos, M. (2019). Cervical Cancer: An Overview of Pathophysiology and Management. Elsevier, 35, 166-174. 10.1016/j.soncn.2019.02.003.
Li, Z., Wu, L., Tan, W., Zhang, K., Lin, Q., Zhu, J., Tu, C., Lv, X., & JIang, C. (2021). MiR-20b-5p promotes hepatocellular carcinoma cell proliferation, migration and invasion by down-regulating CPEB3. Annals of Hepatology. v.23(100345). 10.1016/j.aohep.2021.100345.
Organização das Nações Unidas. Mês de Conscientização do Câncer Cervical: OMS diz que doença pode ser eliminada/ Nações Unidas. - Brasília: ONU, 2020.
Pardini, B., De Maria, D., Francavilla, A., Di Caetano, C., Ronco, G., & Naccarati, A. (2018). MicroRNAs as markers of progression in cervical cancer: a systematic review. BMC Cancer, 18(1), 696. 10.1186/s12885-018-4590-4.
Peng, P., Chen, T., Wang, Q., Zhang, Y., Zhang, F., Huang, S., Tang, Y., Yang, C., Ding, W., Ren, D., Huang, Z., & Guo, Y. (2019). Decreased miR-218–5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer. Oncology Research and Treatment, 42, 165–180.10.1159/000495473.
Piperigkou, Z., Mohr, B., Karamanos, N., & Gotte, M. (2016). Shed proteoglycans in tumor stroma. Cell and Tissue Research, 365(3), 643-55. 10.1007/s00441-016-2452-4.
Rodini, C. L., Da Silva, P. B. C., Assoni, A, F., Carvalho, V.M., & Okamoto, O.K. (2018). Mesenchymal stem cells enhance tumorigenic properties of human glioblastoma through independent cell-cell communication mechanisms. Oncotarget, 15(37) 24766-24777. 10.18632/oncotarget.25346.
Santesso, N., Mustafa, R. A., Wiercioch, W., Keah, R., Gandhi, S., Chen, Y., Cheung, A., Hopkins, J., Khatibs, R., Ma, B., Mustafa, A. A., Lloyd, N., Wu, D., Broutet, N., & Schunemann, H. J. (2015). Systematic reviews and meta-analyses of benefits and harms of cryotherapy, LEEP, and cold knife conization to treat cervical intraepithelial neoplasia. International Journal of Gynecology & Obstetrics, 32 (3), 266-71. 10.1016/j.ijgo.2015.07.026.
Schwarz, D. S., & Blower, M. D. (2016). The endoplasmic reticulum: structure, function and response to cellular signaling. Cellular and Molecular Life Sciences, 73 (1), 79-94. 10.1007/s00018-015-2052-6.
Shen, S., Zang, S., Liu, P., Wang, J., & Du, H. (2020). Potential role of microRNAs in the treatment and diagnosis of cervical cancer. Elsevier, 248, 25-30. doi.org/10.1016/j.cancergen.2020.09.003Stumbar, Sara E., Stevens, Maria, Feld, Z. (2018). Cervical Cancer and Its Precursors. Elsevier, 46(1), 117-134. 10.1002/1097-0142(19951115)76:10+<1902::aid-cncr2820761306>3.0.co,2-0.
Thang, T., Huan, L., Zhang, S., Zhou, H., Gu, L., Chen, X., & Zhang, L. (2017). MicroRNA-212 functions as a tumor-suppressor in human non-small cell lung cancer by targeting SOX4. Oncology Reports, 38(4), 2243-2250. 10.3892/or.2017.5885.
Theocharis, A.D., Shandealis, S. S., Neil, T., Multhaupt, H.A., Hubo, M, frey, H., Goplal, S., Gomes, A., Afratis, N., Lim, C.H., Counchman, J, R., Filmus, J., Sanderson, R. D., Shchaefer, L., Lozzo. R.V., & Karamanos, N.K. (2015). Insights into the key roles of proteoglycans in breast cancer biology and translational medicine. Biochimica et Biophysica Acta,1855 (2), 276-300.10.1016/j.bbcan.2015.03.006.
Tzavlaki, K., & Moustakas, A. (2020). TGF-β Signaling. Biomolecules, 10 (3), 487. 10.3390/biom10030487.
Uliv, P., Canale, M., Passardi, A., Marisi, G., Valgiust, M., Frassineti, G.L., Calistri, D., Amadori, D., Scarpi, E. (2018). Circulating Plasma Levels of miR-20b, miR-29b and miR-155 as Predictors of Bevacizumab Efficacy in Patients with Metastatic Colorectal Cancer. International Journal of Molecular Sciences, 1, 307. 10.3390/ijms19010307.
Wang, K., Chen, M., & Wu, W. (2017). Analysis of microRNA (miRNA) expression profiles reveals 11 key biomarkers associated with non-small cell lung cancer. Journal Of Surgical Oncology, 15, 175. 10.1186/s12957-017-1244-y.
Yang, L., Shi, P., Zhao, G., Xu, J., Peng, W., Zhang, J., Zhang, G., Wang, X., Dong, Z., Chen, F., & Cui, H. (2020). Targeting cancer stem cell pathways for cancer therapy. Signal Transduction and Targeted Therapy - Nature, 7,5 (1), 8.10.1038/s41392-020-0110-5.
Zhang, X., Zhou, J., Xue, D., Li, Z., Liu, Y., & Dong, L. (2019). MiR-515-5p acts as a tumor suppressor via targeting TRIP13 in prostate cancer. International Journal of Biological Macromolecules. 15(129), 227-232. 10.1016/j.ijbiomac.2019.01.127.
Zhou, C., Tan, D., Chen, L., Xu, X., Sun, C., Zong, L., Han, S., & Zhang, Y. (2017). Effect of miR-212 targeting TCF7L2 on the proliferation and metastasis of cervical cancer. European Review for medical and Pharmacological Sciences, 21(2), 219-226.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Larissa Rodrigues de Sousa ; Eleilde Almeida Araujo ; Eldevan da Silva Barbosa ; Thaís da Conceição Silva; Wemerson Matheus Matos Silva; Alania Frank Mendonça; Ana Gabrielly de Mello Matos; Francisca de Brito Araujo Souza; Ana Carla Silva Jansen; Wesliany Everton Duarte; Jaqueline Diniz Pinho
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
