Determination of the chemical profile extracts obtained from Kalanchoe pinnata (Lam.) Pers native of municipality Tabatinga-AM
DOI:
https://doi.org/10.33448/rsd-v11i4.27103Keywords:
Chemical profile; Kalanchoe pinnata; Tabatinga; Chromatography.Abstract
Kalanchoe pinnata formerly known as Bryophyllum pinnatum , is a herbaceous species native to tropical regions such as Africa. In Brazil, it was successfully introduced and propagated, being commonly used in communities far from large capitals. The juice of the leaves is usually used in the treatment of inflammatory diseases, gastric ulcers, burns, diarrhea , vomiting, insect bites and body aches. In the literature, compounds such as anthocyanins and flavonoids are reported that have different biological activities such as antimicrobial, antioxidant, cytotoxic, antitumor, antiparasitic, antiallergic and hepatoprotective . Based on this information and considering that there are few studies on the chemical compounds of K. pinnata in Amazonas , especially in regions farther from the capital, such as in the municipality of Tabatinga. The present work aimed to determine the chemical profile of the fractions of the crude extract of K. pinnata native to the municipality of Tabatinga - AM. For this, plant material was collected from a local producer in the municipality of Tabatinga. Then the leaves were subjected to an extraction by infusion. Soon after, the crude extract obtained was fractionated with hexane, chloroform and ethyl acetate. The resulting fractions were analyzed by High Performance Liquid Chromatography (HPLC) at wavelength λ=254 nm and, using the information available in the literature, it was possible to establish a relationship between the compound quercetin 3- O -α-L- arabinopyranosyl - (1→2)-α-L- rhamnopyranoside , as responsible for the chromatographic peaks observed in the hexane, chloroform, and ethyl acetate fractions. Thus, the present study will serve as a basis for future work on the characterization of chemical compounds present in K. pinnata from the municipality of Tabatinga.
References
Ahn, K. (2017). The worldwide trend of using botanical drugs and strategies for developing global drugs. BMB Reports.
Almeida, A. P., da Silva, S. A., Souza, M. L., Lima, L. M., Rossi-Bergmann, B., de Moraes, V. L., & Costa, S. S. (2000). Isolation and chemical analysis of a fatty acid fraction of Kalanchoe pinnata with a potent lymphocyte suppressive activity. Planta Medica. 66(2), 134-137.
Almeida, Arimar Chagas de. (2019). Atividade antimalárica da Kalanchoe pinnata sobre o Plasmodium berghei em modelo experimental in vivo. Dissertação de Mestrado em Biociências. Universidade Federal do Oeste do Pará – UFOPA.
Araújo, E. R. D. (2017). Kalanchoe brasilienses Cambess e Kalanchoe pinnata (Lamarck) Persoon: caracterização química, avaliação gastroprotetora e anti-inflamatória tópica. Dissertação de mestrado em Ciências Farmacêuticas. Universidade Federal do Rio Grande do Norte.
Araújo, E. R. D., Guerra, G. C. Bernardo, Araújo, D. F. de S., De Araújo, A. A., Fernandes, J. M., Júnior, R. F. De A., Da Silva, V. C., De Carvalho, T. G., Ferreira, L. De S., & Zucolotto, S. M. (2018). Gastroprotective and Antioxidant Activity of Kalanchoe brasiliensis and Kalanchoe pinnata Leaf Juices against Indomethacin and Ethanol- Induced Gastric Lesions in Rats. International. Journal of Molecular Sciences, 19(5), 1265.
Batista, L. A., Brandão, E. G., Rosas, L. V., Pinto, M. N., Pantoja, T. d., De Araújo, T. M., & Lima , R. A. (2019). Levantamento de plantas medicinais utilizadas contra parasitoses e verminoses intestinais no município de Atalaia do Norte-AM. Biota amazônia.
Benchimol, S. (2010). Amazônia: um pouco antes e além-depois. Universidade Federal do Amazonas. (2 ed.).
Biswas, S. K., Chowdhury, A., Das, J., Karmakar, U. K., & Shill, M. C. (2011). Assessment of cytotoxicity and antibacterial activitites of ethanolic extracts of Kalanchoe Pinnata Linn. (Family: Crassulaceae) Leaves and Steams. International Journal of Pharmaceutical Sciences and Research.
Brum, A. A. S., De Arruda, L. F., & Regitano-D´Arce, M. A. B. (2009). Extraction methods and quality of the lipid fraction of vegetable and animal samples. Química Nova, 32(4), 849-854.
Chibli, L. A. (2013). Caracterização química e atividades biológicas de Bryophyllum pinnatum (Lam.) Oken.
Costa, S. S., Jossang, A., Bodo, B., Souza, M. L., & Moraes, V. L. (1994). Patuletin acetylrhamnosides from Kalanchoe brasiliensis as inhibitors of human lymphocyte proliferative activity. Journal of Natural Products, 1503-1510.
Costa, S. S., Muzitano, M. F., Camargo, L. M., & Coutinho, M. A. (2008). Therapeutic Potential of Kalanchoe Species: Flavonoides and other Secondary Metabolites. Natural Product Communications, 2151-2164.
Coutinho, M. A. S., Casanova, L. M., Dos Santos L. B., Leal, D., Palmero, C., Toma, H. K., Dos Santos, E. P., Nasciutti, L. E., & Costa, S. S. (2021). Wound healing cream formulated with Kalanchoe pinnata major flavonoid is as effective as the aqueous leaf extract cream in a rat model of excisional wound. Natural Product Research, 35(24), 6034-6039.
Cruz, E. A., Da Silva, S. A. G., Muzitano, M. F., Silva, P. M. R., Costa, S. S., & Rossi-Bergmann, B. (2008). Immunomodulatory pretreatment with Kalanchoe pinnata extract and its quercitrin flavonoid effectively protects mice against fatal anaphylactic shock. International immunopharmacology, 8(12), 1616-1621.
Do Nascimento, A. L., & Yara, R.. (2015). Isolamento e caracterização de produtos bioativos extraídos de ulomoides dermestoides. Conic XXIII. pp. 4.
El Abdellaoui, S., Destandau, E., Toribio, A., Elfakir, C., Lafosse, M., Renimel, I., Landemarre, L. (2010). Bioactive molecules in Kalanchoe pinnata leaves: extraction, purification, and identification. Analytical and Bioanalytical Chemistry, pp. 1329-1338.
Embrapa. (2014). embrapa.br. Acesso em 23 de 01 de 2022, disponível em Contando Ciência: https://www.embrapa.br/contando-ciencia/bioma-amazonia
Ferreira, R. T., Coutinho, M. A. S., Malvar, D. do C., Costa, E. A., Florentino, I. F., Costa, S. S., & Vanderlinde, F. A. (2014). Mechanisms underlying the antinociceptive, antiedematogenic, and anti-inflammatory activity of the main flavonoid from Kalanchoe pinnata. Evidence-Based Complementary and Alternative Medicine (v.2).
Gonçalves, F. S. M. (2017). Mecanismos de ação relacionados à atividade antiúlcera de Kalanchoe pinnata (Lam.) Pers. (Crassulaceae). Universidade de São Paulo.
Higuchi, M. I. (2004). A floresta amazônica e suas múltiplas dimensões: uma proposta de educação ambiental. INPA / CNPq.
Ichikawa, M., Ogura, M., & Lima, T. (1986). Antiallergic flavone glycoside from Kalanchoe pinnatum.
Kamboj, A., & Saluja, A. K. (2009). Bryophyllum pinnatum (Lam.) kurz: phytochemical and pharmacological profile: A review. Pharmacognosy Review, pp. 364-375.
Klitgaard, A., Iversen, A., Andersen, M. R., Larsen, T. O., & Frisvad, J. C. (2014). Aggressive dereplication using UHPLC–DAD–QTOF: screening extracts for up to 3000 funga secondary metabolitesl. Anal Bioanal Chem, pp. 1933-1943.
Lanna, E. G., moreira, A. M. S., bittencourt, V. C. S., Sousa, O. V., & Denadai, A. M. L. (2019). Avaliação da atividade antioxidante de frações de Bryophyllum pinnatum (Lam.) Oken incorporadas em β-ciclodextrina. Revista Virtual de Química, 11(4).
Lisboa, M. S., Ferreira, S. M., & Silva, M. S. (2006). Uso de plantas medicinais para tratar úlceras e gastrites pela comunidade do povoado vila do capim, Município de Arapiraca-Al, Nordeste do Brasil. Setienbulus Série Ciências Biológicas (Etnobiologia).
Lorenzi, H., & Matos, F. J. (2008). Plantas medicinaisno Brasil: nativas exóticas cultivadas. (2a ed.), Instituto Plantarum de Estudos da Flora.
Marques, L. C. (2005). Preparação de extratos vegetais. Jornal Brasileiro de Fitomedicina, 3(2), 74-76.
Muzitano, Michelle F., Bergonzic, M. C., De Melo, G. O., Lage, C. L. S., Bilia, A. R., Vincieric, F. F., Rossi-Bergmann, B., & Costa, S. S. (2011). Influence of cultivation conditions, season of collection and extraction method on the content of antileishmanial flavonoids from Kalanchoe pinnata. Journal of Ethnopharmacology, 133(1), 132-137.
Muzitano, Michelle F., Tinoco, L. W., Guette, C., Kaiser, C. R, Rossi-Bergmann, B., & Costa, S. S. (2006) The antileishmanial activity assessment of unusual flavonoids from Kalanchoe pinnata. Phytochemistry, 67(18), 2071-2077.
Okwu, D. E., & Nnamdi, F. U. (2011). A novel antimicrobial phenanthrene alkaloid from Bryophllum pinnatum. Journal of Chemical and Pharmaceutical Research, 27-33.
Oliveira, V. B., Zuchetto, M., Oliveira , C. F., Paula, C. S., Duarte, A. S., Miguel, M. D., & Miguel, O. G. (2016). Efeito de diferentes técnicas extrativas no rendimento, atividade antioxidante, doseamentos totais e no perfil por clae-dad de dicksonia sellowiana (presl.). Hook dicksoniaceae. Revista Brasileira de Plantas Medicinais, 230-239.
Pedroza, M. S., Carvalho, C. M., Sanchez, C. T., & Silva, I. O. (2017). Conhecimento etnobiológico sobre o uso de plantas medicinais e ensino de biologia: aproximações iniciais. Latin American Journal of Science Education.
Rodrigues, F. A., Pimenta, V. C., Braga, K. D., & De Araújo, E. G. (2016). Obtenção de extratos de plantas do cerrado. Enciclopédia biosfera, 870.
Silva, R. Y. A. (2019). Estudo dos efeitos de espécies vegetais da relação nacional de plantas medicinais de interesse ao SUS sobre o receptor PXR.
Skoog, D. A., Holler, W. D., F. J., & Crouch, S. R. (2010). Fundamentos de Química Analítica (8a ed.). Cengage Learning.
Smith, G. (2004). Kalanchoe species poisoning inpets. Vet. Medic., 933-936.
Snafi, A. S. (2013). The Chemical Constituents and Pharmacological Effects of Bryophyllum calycinum. A review. International Journal of Pharma Sciences and Research.
Soares, A. M. da S. (2017). Propriedade anti-inflamatória de Kalanchoe pinnata pode estar associada à inibição nitrérgica e a ação antioxidante. Universidade Federal do Oeste do Pará.
Sobreira, F. C. (2013). Avaliação da atividade antiúlcera de Kalanchoe pinnata (Lam.) Pers (Crassulaceae). Universidade de São Paulo.
Sousa, P. C., Rocha, J. S., Pessoa, A. M., & Carvalho, J. C. (2005). Estudo preliminar da atividade anti-inflamatória de Bryophillum calycium Salisb. Revista Brasileira de Farmacognosia, 60-64.
Tatsimo, S. J. N., Tamokou, J. De D., Havyarimana, L., Csupor, D., Forgo, P., Hohmann, J., Kuiate, J., & Tane, P. (2012). Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. BMC Research notes, 5(1), 1-6.
Wehrens, R., Carvalho, E., Masuero, D., de Juan, A., & Martens, S. (2013). High-throughput carotenoid profiling using multivariate curve resolution. Anal Bioanal Chem, 5075-5086.
Wright, S. J. (2002). Plant diversity in tropical forests: a review of mechanisms of species coexistence. Oecologia, 130.
Zucchi, M. R., Júnior-Oliveira, V. F., Gussoni, M. A., Silva, M. A., & Marques, N. E. (2013). Levantamento etnobotânico de plantas medicinais na cidade de Ipameri - GO. Revista Brasileira de Plantas Medicinais.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Caroline Vieira Alves; Sarah Raquel Silveira da Silva Santiago; Elzalina Ribeiro Soares; Richardson Alves de Almeida; Bruna Ribeiro de Lima; Cristiane Suely Melo de Carvalho; Paulo Alexandre Lima Santiago
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
