Individuals in an endemic region for Leishmania braziliensis display lower levels of CD45RO in T cells

Authors

DOI:

https://doi.org/10.33448/rsd-v11i5.28255

Keywords:

Flow cytometry; Leishmaniasis; Cutaneous; Neglected disease; T-Lymphocytes.

Abstract

Cutaneous Leishmaniasis (CL) is an important neglected tropical disease, that reaches about 900 thousand to 1.5 million people annually, mainly in Brazil.  The present study aimed to characterize these lymphocyte populations and the expression of the CD45RO marker profile in patients with CL. We evaluated CD4+, CD8+, double-positive (DP) and double-negative (DN) T cells and their expression of CD45RO in Cutaneous Leishmaniasis patients, divided into three groups: before treatment (BT), posttreatment (PT) and subclinical (SC). CD4+ T cells had a higher percentage in PT and SC groups when compared to the control group (CT). CD4+ T cells presented a greater expression of CD45RO in BT and PT groups compared to the SC group, that had a lower expression compared with the CT. CD8+ T lymphocytes had a higher percentage in BT when compared with CT and a greater expression of CD45RO when compared to PT, SC and CT groups. We did not observe any statistical significance in the percentage of the DP and DN lymphocytes, however, we noticed that the DP lymphocytes presented a lower expression of CD45RO in the SC group when compared to the CT group, whereas the DN lymphocytes showed a greater expression of CD45RO in the BT group when compared with the other groups. CD4+ T cells appear to be protective in these patients and CD8+ T lymphocytes seem to be associated to pathogenesis, while DP CD45RO+ and DN CD45RO+ lymphocytes appear to play a dual role.

Author Biographies

Marton Kaique de Andrade Cavalcante, Instituto Aggeu Magalhães; Universidade Federal de Pernambuco

Marton Kaique de Andrade Cavalcante graduated in Nursing from the Federal University of Pernambuco (UFPE), has a master's degree (2020) in Therapeutic Innovation from UFPE. He is currently doing a PhD in Therapeutic Innovation from UFPE. Since his scientific initiation, he has been working on the immune response in patients with cutaneous leishmaniasis.

Rafael de Freitas e Silva, Universidade de Pernambuco

Rafael de Freitas e Silva graduated in Biomedicine from the Federal University of Pernambuco (UFPE), has a master's degree in Nuclear Energetic Technologies from UFPE and a doctorate in Therapeutic Innovation from UFPE. Since 2012, He is Adjunct Professor of Immunology at University of Pernambuco (UPE).

Valéria Rêgo Alves Pereira, Instituto Aggeu Magalhães

Valéria Rêgo Alves Pereira has a PhD in Cellular and Molecular Biology from the Oswaldo Cruz Foundation (2003). Currently she is a Senior Technologist in Public Health and responsible for the Flow Cytometry Core unit at Aggeu Magalhães Institute (IAM/FIOCRUZ/PE). She is part of the Ethics Committee in Human Research of IAM and a professor on the Post-Graduation programs of Therapeutic Innovation (UFPE) and Health Biosciences and Biotechnology (IAM). She leads the research group of Immunology of Infectious and Parasitic Diseases, has expertise in Immunology with emphasis in Immunoparasitology, acting mainly on the studies of the cellular and humoral responses of individuals who are susceptible to infectious and parasitic diseases in different stages of the pathological process, mainly with leishmaniasis.

Maria Carolina Accioly Brelaz-de-Castro, Universidade Federal de Pernambuco

Maria Carolina Accioly Brelaz-de-Castro graduated in Biomedicine from the Federal University of Pernambuco (UFPE), has a master's degree in Sciences from the Aggeu Magalhães Institute (IAM) and a doctorate (2013) and post-doctorate (2015) in Therapeutic Innovation from UFPE. She is currently a professor at UFPE and at the postgraduate program in Therapeutic Innovation, from the same University. She is a collaborator of IAM and a member of their flow cytometry core unit. She has experience in the fields of Immunology and Parasitology and her research group is focused on the development of immunological biomarkers and vaccines for infectious and parasitic diseases, mainly working with leishmaniasis.

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Published

04/04/2022

How to Cite

CAVALCANTE, M. K. de A. .; REHN, V. N. C. .; BRITO, M. E. F. de .; SILVA, R. de F. e .; PEREIRA, V. R. A. .; BRELAZ-DE-CASTRO, M. C. A. . Individuals in an endemic region for Leishmania braziliensis display lower levels of CD45RO in T cells. Research, Society and Development, [S. l.], v. 11, n. 5, p. e22811528255, 2022. DOI: 10.33448/rsd-v11i5.28255. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/28255. Acesso em: 26 apr. 2024.

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Vol. 11 No. 5

Section

Health Sciences

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Copyright (c) 2022 Marton Kaique de Andrade Cavalcante; Vitorina Nerivânia Covello Rehn; Maria Edileuza Felinto de Brito; Rafael de Freitas e Silva; Valéria Rêgo Alves Pereira; Maria Carolina Accioly Brelaz-de-Castro

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