Prevalence of Chlamydia trachomatis in women attended by an Extension Program at a public university in Belém, in the State of Pará: a pilot study

Authors

DOI:

https://doi.org/10.33448/rsd-v11i5.28332

Keywords:

C. trachomatis; Saúde da Mulher; Esino; Comunidade.

Abstract

Chlamydia trachomatis is a sexual bacterium that causes about 92 million new cases annually worldwide and can result in Pelvic Inflammatory Disease (PID) and infertility. The objective of this study was to determine the prevalence of sexual infection by C. trachomatis in women assisted by an extension project at a public university in Belém, state of Pará. This is a cross-sectional and prospective pilot study that included women attended from January to March 2019. Social, epidemiological and gynecological complaints variables were collected in a specific questionnaire and endocervical secretion was collected during the Pap smear. DNA extraction was performed using a standardized kit and its control was performed by the 268bp Polymerase Chain Reaction (PCR) of the human β-globin gene. To detect the infection, 394bp of the ompA gene of C. trachomatis was amplified by PCR. The odds ratio test was used to verify the risk of infection, according to the variables studied. The p-value ≤0.05 (95% confidence interval) was considered for the significance of the analyses. The prevalence of infection was 3.4% in the 86 women investigated, who had a mean age of 42.9 years ± 11.7 years. None of the investigated variables stood out significantly, probably due to the low sample size. The prevalence of sexual infection by C. trachomatis is low in older women.

References

Anyalechi, G. E., Hong, J., Danavall, D. C., Martin, D. L., Gwyn, S. E., Horner, P. J., Raphael, P. H., Kirkcaldy, R. D., Kersh, E. N. K. & Bernstein, T. (2021). High Plasmid Gene Protein 3 (Pgp3) Chlamydia trachomatis Seropositivity, Pelvic Inflammatory Disease, and Infertility Among Women, National Health and Nutrition Examination Survey, United States, 2013-2016. Clin Infect Dis. 73(8):1507-1516. doi: 10.1093/cid/ciab506.

Azevedo, M. J. N., Nunes, S. S., Oliveira, F. G. & Rocha, D. A. P. (2019). High prevalence of Chlamydia trachomatis in pregnant women attended at Primary Health Care services in Amazon, Brazil. Rev Inst Med Trop Sao Paulo; 61: e6. Published online 2019 Feb 14. doi: 10.1590/S1678-9946201961006.

Brasiliense, D. M., Borges, B. N. & Ferreira, W A. (2016). Genotyping and prevalence of Chlamydia trachomatis infection among women in Belém, Pará, northern Brazil. J Infect Dev Ctries. 10(2):134-7.

Brasil (2016). estratégia de rastreamento do câncer do colo uterino. Ministério da saúde.

Brasil. Informe técnico sobre a vacina papilomavírus na atenção básica. Secretaria de Vigilância em Saúde, Departamento de Vigilância de Doenças Trnasmissíveis, Coordenação Geral do Programa Nacional de Imunizações, Ministério da Saúde, 2014.

Center for Disease Control and Prevention. (2021) Sexually Transminted Diseases. Sexually Transmitted Infections Prevalence, Incidence, and Cost Estimates in the United States. https://www.cdc.gov/std/chlamydia/default.htm

Cazanave, C. & Barbeyrac, B. (2019). Pelvic inflammatory diseases: Microbiologic diagnosis - CNGOF and SPILF Pelvic Inflammatory Diseases Guidelines. Gynecol Obstet Fertil Senol.47(5):409-417. doi: 10.1016/j.gofs.2019.03.007.

European Centre for Disease Prevention and Control. (2020). Chlamydia infection. Disponible https://www.ecdc.europa.eu/en/chlamydia-infection

Costa-Lira, E., Jacinto, A. H. V. L., Silva, L. M., Napoleão, P. F. R., Barbosa-Filho, R. A. A., Cruz, G. J. S., Astolfi-Filho, S. & Borborema-Santos, C. M. (2017). Prevalence of human papillomavirus, Chlamydia trachomatis, and Trichomonas vaginalis infections in Amazonian women with normal and abnormal cytology. Genet Mol Res.16(2). doi: 10.4238/gmr16029626.

Curry, A., Williams, W. & Penny, M. L. (2019). Pelvic Inflammatory Disease: Diagnosis, Management, and Prevention. Am Fam Physician.100(6):357-364.

He. W., Jin, Y., Zhu, H., Zheng, Y. & Qian, J. (2020). Effect of Chlamydia trachomatis on adverse pregnancy outcomes: a meta-analysis. Arch Gynecol Obstet. Sep;302(3):553-567. doi: 10.1007/s00404-020-05664-6. Epub 2020 Jul 8.

Hunter, L.A., Nelson, L., Chow, J. M., Holt, B. Y. & Bauer, H. M. (2018). Contraceptive Method Use and Chlamydia Positivity Among California Family Planning Clients: The Case for New Multipurpose Prevention Technologies. J Womens Health (Larchmt). 27(6):768-774. doi: 10.1089/jwh.2017.6558.

INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA (IBGE) (2021). https://www.ibge.gov.br/

Jalal, H.., Stephen, H., Alexander. S., Carne, C. & Sonnex C. (2007). Development of real-time PCR assays for genotyping of Chlamydia trachomatis. Journal of Clinical and Microbiology, 45:2649-53.

Last, A. R., Pickering, H., Roberts, C. H., Coll, F., Phelan, J., Burr, S. E., Cassama, E., Nabicassa, M., Hadfield, J., Cutcliffe, L. T., Clarke, I. N., Mabey, D. C. W., Bailey, R. L., Clark, T. G., Thomson, N. R. & Holland, M. J. (2018). Population-based analysis of ocular Chlamydia trachomatis in trachoma-endemic West African communities identifies genomic markers of disease severity. Genome Med. Feb 26;10(1):15. doi: 10.1186/s13073-018-0521-x.

Machado, L. F. A., Fonseca, R. R. S., Queiroz, M. A. F., Oliveira-Filho, A. B., Vallinoto, I. M. V. C., Vallinoto, A. C. R., Ishak, M. O. G. I. & Ishak, I. (2021). The Epidemiological Impact of STIs among General and Vulnerable Populations of the Amazon Region of Brazil: 30 years of Surveillance. Viruses. 2021 May; 13(5): 855. doi: 10.3390/v13050855.

Martins, B. G., Silva, W. R., Marôco, J. & Campos, J. A. D.B . (2021). Eating Behavior of Brazilian College Students: Influences of Lifestyle, Negative Affectivity, and Personal Characteristics. Percept Mot Skills. 128(2):781-799. doi: 10.1177/0031512520983082.

Peuchant, O., Touati, A., Nadalié, C. L., Hénin, N., Cazanave, C., Bébéar, C. & Barbeyrac, B. (2020). Prevalence of lymphogranuloma venereum among anorectal Chlamydia trachomatis-positive MSM using pre-exposure prophylaxis for HIV. Sex Transm Infect. 96(8):615-617. doi: 10.1136/sextrans-2019-054346.

Rawre, J., Dhawan, B., Khanna, N., Sreenivas, V., Broor, S. & Chaudhry, R. (2019). Distribution of Chlamydia trachomatis omp A genotypes in patients attending a sexually transmitted disease outpatient clinic in New Delhi. India. Indian J Med Res. 149(5):662-670. doi: 10.4103/ijmr.IJMR_1171_17.

Ribeiro, A. A., Saddi, V. A., Carneiro, M. A., Alves, R. R. F., Barros, N. K. S., Carvalho, K. P. A. C., Tavares, S. B. N., Teles, S. A., D'Alessandro, W. B. & Santos, S. H. R. (2020). Human Papillomavirus and Chlamydia Trachomatis Infections in Adolescents and Young Women: Prevalence and Risk Factors. Diagn Cytopathol. doi: 10.1002/dc.24460.

Rocha, D. A. P., Barbosa-Filho, R. A. A., Mariño, J. M. & Santos, C. M. B. (2014). Hidden” sexually transmitted infections among women in primary care health services, Amazonas, Brazil. International Journal of STD & AIDS. 25:878-86.

Rodrigues, L .L. S., Hardick, J., Nicol, A. F., Morgado, M. G., Martinelli, K. G., Paula, V. S., Pilotto, J. H. & Gaydos, C. A. (2019). Sexually transmitted infections among HIV-infected and HIV-uninfected women in the Tapajós region, Amazon, Brazil: Self-collected vs. clinician-collected samples. PLoS One. 14(4):e0215001. doi: 10.1371/journal.pone.0215001.

Silveira, M. F., Bruni, M. P., Stauffert, D., Golparian, D. & Unemo, M. (2020). Prevalence and Risk Factors Associated With Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium Among Women in Pelotas, Southern Brazil. Int J STD AIDS.

Santos, L. M., Ulian, W. L., Trindade, J. Q, Sousa, F. D. M., Oliveira, J. F. G., Pereira, C .C. C., Brasiliense, D. M., Prazeres, B. A., Tsutsumi, M. Y., Ishikawa, E. A. Y. & Sousa, M. S. (2017). Prevalência da infecção endocervical de Chlamydia trachomatis em universitárias do Estado do Pará, região Amazônica, Brasil. Revista Pan-Amazônica de Saúde (Online), V. 8, P. 27-33. Doi:dx.doi.org/10.5123/s2176-62232017000300004.

Santos, L. M., Souza, I. R. A., Holanda, L. H. C., Vaz, J. O., Tsutsumi, M. Y., Ishikawa, E. A. Y. & Sousa, M .S. (2016). Alta incidência da infecção urogenital por Chlamydia trachomatis em mulheres parturientes de Belém, Estado do Pará, Brasil. Revista Pan-Amazônica de Saúde (Online), v. 7, p. 101-106. Doi:dx.doi.org/10.5123/s2176-62232016000400012

Santos, L .M., Vieira, M. R. M. D .S., Oliveira, J. F. G., Trindade, J. Q., Brasiliense, D. M., Ferrari, S. F., Tsutsumi, M. Y., Fuzii, H. T., Sousa Junior, E. C., Ishikawa, E. A. Y., Ishak, R. & Sousa, M. S. (2018). High prevalence of sexual Chlamydia trachomatis infection in young women from Marajó Island, in the Brazilian Amazon PLoS One, 13: e0207853.

Suehiro, T. T., Gimenes, F., Souza, R. P., Taura, S. K. I., Cestari, R. C. C., Irie, M. M. T., Boer, C. G., Consolaro, M. E. L. & Silva, V. R .S. D. (2021). High molecular prevalence of HPV and other sexually transmitted infections in a population of asymptomatic women who work or study at a Brazilian university. Rev Inst Med Trop Sao Paulo. 63:e1. doi: 10.1590/S1678-9946202163001.

Sukatendel, K., Mayniar, T. E., Aboet, A., Adela, C. A., Lumbanraja, S., Ichsan, T. M. & Edianto, D. (2019). Relationship between Chlamydia Trachomatis Infection with Patency Tubal and Non-Patency Tubal Occurrence in Infertile Women. Maced J Med Sci .7(20):3437-3442. doi: 10.3889/oamjms.2019.440.

Tang, W., Mao, J., Li, K. T., Walker, J. S., Chou, R., Fu, R., Chen, W., Darville, T., Klausner, J. & Tucker, J. D. (2020). Pregnancy and fertility-related adverse outcomes associated with Chlamydia trachomatis infection: a global systematic review and meta-analysis. Sex Transm Infect. 96(5):322-329. doi: 10.1136/sextrans-2019-053999.

Travassos, A. G., Xavier-Souza, E., Netto, E., Dantas, E. V., Timbó, M., Nóbrega, I., Haguihara, T., Neumeyer, J., Lisboa, N., Soidan, M. A., Ferreira, F. & Brites, C. (2016). Anogenital Infection by Chlamydia Trachomatis and Neisseria Gonorrhoeae in HIV-infected Men and Women in Salvador, Brazil. Braz J Infect Dis. 20(6):569-575. doi: 10.1016/j.bjid.2016.09.004.

Torrado, M., Nicolau, L. B., Skryabin, V., Teixeira, M., Eusébio, S., Ouakinin. S. (2020). Emotional dysregulation features and problem gambling in university students: a pilot study. J Addict Dis. 38(4):550-566. doi: 10.1080/10550887.2020.1800889.

Wu, L., Wang, J., Gao, Y. & Zhu, L. (2020). Different patterns of perceived barriers to psychological treatment among Chinese depressed college students: Preliminary findings. J Clin Psychol. 2020 Jul;76(7):1339-1352. doi: 10.1002/jclp.22938. PMID: 32020638 DOI: 10.1002/jclp.22938.

Published

06/04/2022

How to Cite

SANTOS, L. M. dos; SILVA, M. C. da .; TRINDADE , J. de J. Q. .; VIEIRA, R. C. .; ORTIZ, R. M. S. .; SILVA, D. R. da .; VIRGOLINO, R. R. .; BRASILIENSE , D. M. .; ISHIKAWA, E. A. Y.; SOUSA, M. S. de . Prevalence of Chlamydia trachomatis in women attended by an Extension Program at a public university in Belém, in the State of Pará: a pilot study. Research, Society and Development, [S. l.], v. 11, n. 5, p. e27411528332, 2022. DOI: 10.33448/rsd-v11i5.28332. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/28332. Acesso em: 22 dec. 2024.

Issue

Section

Health Sciences