Evaluation of the mutagenic and antimuutagenic activity of Chlorella vulgaris in a test of Allium cepa
DOI:
https://doi.org/10.33448/rsd-v11i5.28346Keywords:
Microalgae; Copper sulfate; Mutagenicity test; DNA damage.Abstract
The microalgae Chlorella vulgaris is usually commercialized as nutraceutical although it has potential application in the pharmaceutical and cosmetic industries. Therefore, our objective in this research is to evaluate the mutagenic and antimutagenic action of the aqueous extract of C. vulgaris through the Allium cepa assay. Three concentrations of the aqueous extract of C. vulgaris were tested: 0.075, 0.15 and 0.30mg/mL. In the mutagenicity analysis, A. cepa meristematic cells were cultured in the presence of the aqueous extract of C. vulgaris with distilled water as negative control and copper sulfate as positive control. For antimutagenicity, pre-treatment, simple simultaneous and post-treatment protocols were used. 400 cells/treatment were analyzed under optical microscopy (40x). Data were analyzed by ANOVA (one-way) and Tukey tests, considering p<0.05. The aqueous extract of C. vulgaris did not show mutagenicity in any of the three concentrations evaluated. About the antimutagenicity protocols, the harm reduction percentages were 94.7%, 94.1% and 96.2% (pre-treatment); 88.9%, 93.2% and 91.08% (simultaneous simple); and 85.2%, 84.5% and 94.7% (post-treatment) referring to concentrations of 0.075, 0.15 and 0.30 mg/mL, respectively. According to these results, the microalgae C. vulgaris did not show mutagenic action at the tested doses and it reduced genetic damage caused by copper sulfate.
References
Agertt, F. M. dos S., Martins, C. T. A., Gutknecht, M. M., & Fachinetto, J. M. (2021). Análise da citotoxicidade dos arroios espinho e moinho do município de Ijuí, RS. Salão do Conhecimento, XXIX Seminário de Iniciação Científica, Ijuí, Santa Rosa, Panambi, Três Passos, RS, Brasil. https://publicacoeseventos.unijui.edu.br/index.php/salaoconhecimento/article/view/20941/19652
Aggarwal, A. N. D. (2020). Fígado: detox, gordura no fígado & doenças crônicas: dieta & remédios naturais para o fígado, síndrome do intestino permeável, perda de peso, saúde mental, hormônios, câncer & cuidados com a pele. Victoria, Canadá: AbeBooks.
Anciã, J. P., & Romão, N. F. (2016). Analysis da atividade citotóxica e mutagênica do extrato aquoso da parte aérea de Uncaria tomentosa em um teste de Allium cepa SAJEBTT, 3 (2), 16-26. https://periodicos.ufac.br/index.php/SAJEBTT/article/view/195
Bezerra, R. P., de Arruda M. C. S., de Andrade Calaça, P. R., Cavalcanti, V. L. R., Brandão, R. M. P. C., & Porto, A. L. F. (2021). Revisão integrativa sobre atividade antitumoral de microalgas e cianobactérias. Revista Valore, 6, e-6001. https://doi.org/10.22408/reva602021554e-6001
Bischoff, H. W., & Bold, H. C. (1963). Phycological studies. IV. Some soil algae from enchanted rock and related algal species. Univ Texas Publ, 6318, 95.
Bispo, R. B., Cardoso, E. dos S., Sander, N. L., de Arruda, J. C., Rodrigues, A. S., & et al. (2021). Citogenotoxicidade de extratos aquosos de Erythrina fusca Lour. sobre o ciclo celular de Allium cepa L Citotoxity and genotoxity of aqueous extracts of Erythrina fusca Lour. about the cell cycle of Allium cepa L. Brazilian Journal of Development, 7, 10, 99270-99285. https://doi.org/10.34117/bjdv7n10-308
Boukid, F., & Castellari, M. (2021). Foods and beverages containing algae and derived ingredients launched on the market from 2015 to 2019: a front-of-pack labeling perspective with a special focus on Spain. Foods, 10 (1), 173. https://doi.org/10.3390/foods1001017333467009
Cavalcanti, V. L. Ribeiro, R. M. P. B. -C., E., Viana, P. A., Frazão, de A., Câmara, A. L., Porto, A. L. F., & Bezerra, R. P. (2021). Chlorella vulgaris lectin kills Aedes aegypti larvae. Algal Research, 56, 102290. https://10.1016/j.algal.2021.102290
Chen, X., Song, L., Wang, H., Song, L., Huahua, Y., Wang, X., Rongfeng, L., Tianzhong, L., & Pengcheng, L. (2019). Partial characterization, the immune modulation and anticancer activities of sulfated polysaccharides from filamentous microalgae Tribonema sp. Molecules, 24 (2). https://doi:10.3390/molecule24020322
Chu C.-Y., Huang, R., & Ling, L. -P. (2006). Purification and characterization of a novel haemagglutinin from Chlorella pyrenoidosa. J Ind Microbiol Biotechnol, 33 (11), 967. https://doi.org/10.1007/s10295-006-0145-9
Costa, M., Garcia, G., Costa-Rodrigues, J., Costa, M. S., Ribeiro, M. J., Fernandes, M. H., & et al. (2014). Exploring bioactive properties of marine Cyanobacteria isolated from the Portuguese coast: High potential as a source of anticancer compounds. Mar Drugs, 12 (1), 98-114. https://doi.org/10.3390/md12010098
Da Silva, A. E. P., Moura, J. W. M., & Lucio Neto, M. P. (2015). Avaliação tóxica, citotóxica, genotóxica e mutagênica do composto 3- (2-cloro-6-fluorobenzil) - imidazolidina-2,4-diona em células eucarióticas. Rev. Saúde em Foco, 2 (1), 25-48. www4.unifsa.com.br/revista/index.php/saudeemfoco/article/view/694
Dantas, D. M. M., Cahú, T. B., Oliveira, C. Y. B., Abadie-Guedes, R., Roberto, et al. (2021) Chlorella vulgaris functional alcoholic beverage: Effect on propagation of cortical spreading depression and functional properties. PLoS ONE 16, (8), e0255996. https://doi.org/10.1371/journal.pone.0255996
Dextro, R. B. (2019). Efeitos do cobre na microalga Kirchneriella obesa em cultura unialgal e em co-cultivo com Chlorella sorokiniana. Dissertação de mestrado, Universidade Federal de São Carlos, São Carlos, SP, Brasil. repositorio.ufsc.br/handle/123456789/226455
Dos Santos, J. F. L., Cardoso, E. S., dos Santos, I. R. B., Pena, G. F., & et al. (2021). Potencial citogenotóxico de Byrsonima crassifolia (murici), Malpighiaceae. Brazilian Journal of Development, 7 (3), 32905-32917. https://doi.org/10.34117/bjdv7n3-828
Echeveste, P., Silva, J. C., & Lombardi, A. T. (2017). Cu and Cd affect distinctly the physiology of a cosmopolitan tropical freshwater phytoplankton. Ecotoxicol Environ Saf, 143, 228-235. https://doi.org/10.1016/j.ecoenv.2017.05.030
Fachinetto, J. M., Bagatini, M. D., Durigon, J., da Silva, A. C. F., & Tedesco, S. B. (2007). Anti-proliferative effect of Achyrocline satureioides DC (Asteraceae) infusions on the cell cycle of Allium cepa. Rev Bras Farmacogn; 17 (1), 49-54. https://doi.org/10.1590/S0102-695X2007000100011
Ferreira, W. A., Aguiar, G. S., Pessoa, H. R., da Costa, D. C. F., & Zago, L. (2021). Potencial antitumoral dos compostos fenólicos de produtos da oliveira (Olea europaea L.): uma revisão integrativa da literatura. Research, Society and Development, 10, 13, e22101320733-e22101320733. https://doi.org/10.33448/rsd-v10i13.20733
Figueira, A. C. G. (2017). Avaliação das atividades angiogênica / antiangiogênica e mutagênica / antimutagênica do óleo essencial de Lantanacamara (Cambará). Dissertação de mestrado, Universidade Católica de Goiás, Goiânia, Brasil, 2017. Availablefrom:tede2.pucgoias.edu.br:8080/handle/tede/3713
Islam, M. T., Streck, L., de Alencar, M. V., Cardoso Silva, S. W., da Conceição M. Kátia, da Conceição M. Keylla, & et al. (2017). Evaluation of toxic, cytotoxic and genotoxic effects of phytol and its nanoemulsion. Chemospherel, 77, 93-101. https://doi.org/10.1016/j.chemosphere.2017.02.145
Kolumbayeva, S., Dzhokebayeva, S., Begimbetova, D., & Lovinskaya, A. (2014). Antimutagenic properties of biologically active substances of microalgae associates. Cent Asian J Glob Health, 12 (Suppl 3), 162. https://doi.org/10.5195/cajgh.2014.162
Langston, W. J. (1990). Toxic effects of metals and the incidence of metal pollution in marine ecosystems. in: furness, r. w. &, rainbow, p. s. (eds.). heavy metals in the marine environment, 1rdedn, (1-3). boca raton: crc press, usa.
Macedo, W., Mello, V., Santos, B. N., Fernandes, L., & Karsburg, I. (2018). Efeito citotóxico e genotóxico de Crescentia cujete L. (BIGNOCIACEAE) através do bioteste Allium cepa. Agrarian Academy, 5 (10). https://conhecer.org.br/ojs/index.php/agrarian/article/view/5009
Magalhães, D. P. (2014). Avaliação química e ecotoxicológica para a seleção de bioensaios aquáticos sensíveis a efluentes contendo metais. Tese de doutorado, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brasil. www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8960
Marques, D. M., Silva, A. B., Mantovani, J. R., Pereira, D. S., & Souza, T. C. (2018). Growth and physiological responses of tree species (Hymenaea courbaril L., Peltophorum dubium (Spreng.) Taub. and Myroxylon peruiferum L. F.) exposed to different copper concentrations in the soil. Revista Árvore, 42 (2), 62-67. https://doi.org/10.1590/1806-90882018000200002
Meneguetti, D. U. O., Lima, R. A., da Silva, J. B., Silva, R. P., Pagotto, R. C., & Facundo, V. A. (2014). Cytotoxic and mutagenic analysis of the aqueous extract of maytenus guyanensis klotzsch ex Reissek (Celastraceae) chichuá (xixuá) Amazon. Ci and Nat, 36 (3). https://301-309. doi.org/10.5902/2179460X13343
Mesadri, J., Wagner, R., & Fagundes, M. B. (2021). Potencial das microalgas na indústria farmacêutica. In: Severo, I. A., do Nascimento, T. C., & Fagundes, M. B. (Orgs.). Microalgas: potenciais aplicações e desafios (pp. 45-62). Canoas: Mérida. https://doi.org/10.4322/mp.978-65-994457-8-1.c2
Messias, J. B., de Brito, R. L. Beltrão, G. T. de A., Messias, I. M. de O., Florêncio, M. S., & et al. Citogenotoxicidade e mutagenicidade do sulfato de cobre em diferentes variedades de allium cepa linn. Brazilian Journal of Development 7, (9), 88231-88244. https:// 10.34117/bjdv7n9-131
Morcelli, A. V. (2021). Aplicação de tecnologias de extração de clorofilas e carotenoides de microalgas e uso da biomassa microalgal na adsorção de metais pesados. Tese de doutorado, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil. http://hdl.handle.net/10183/231595
Pinto, E., Sigaud-kutner, T. C. S., Leitão, M. A. S., Okamoto, O. K., Morse, D., & Colepicolo, P. (2003), Heavy metal–induced oxidative stress in algae. Journal of Phycology, 39, 1008-1018. https://doi.org/10.1111/j.0022-3646.2003.02-193.x
Soares, L. S. (2021). Revisão teórica: cultivo de microalgas para a produção de carotenoides. Monografia, Universidade Estadual Paulista – Unesp, São Paulo, Brasil. http://hdl.handle.net/11449/216523.
Okuyama, N. C. M., Biazi, B. I., Fujita, T. C., Gualtieri, K. A., & Oliveira, R. J. (2018). Avaliação dos efeitos mutagênicos e antimutagênicos do fator de crescimento Chlorella growth factor (cgf) pelo ensaio de Allium cepa. Terra & Cultura, 29 (57), 23-30. periodicos.unifil.br/index.php/Revistateste/article/view/171
Oliveira, C. Y. B., Oliveira, C. D. L., Prasad, R., Ongh, C., Araujo, E. S., Shabnam, N., & Gálveza, O. (2021). A multidisciplinary review of Tetradesmus obliquus: a microalga suitable for large-scale biomass production and emerging environmental applications. Reviews in Aquaculture, 13 (3), 1594-1618. https://doi.org/10.1111/RAQ.12536
Osuna-Ruiz, I., López-Saiz, C. M., Burgos-Armando, A., Velázquez, C., Nieves-Soto, M., & Hurtado-Oliva, M. A. (2016). Antioxidant, antimutagenic and antiproliferative activities in selected seaweed species from Sinaloa, Mexico, Pharm Biol, 54 (10), 2196-2210. https://doi.org/10.3109/13880209.2016.1150305
Qin, R., Wang, D., Chen, L. O., Björn, L. O., & Li, S. (2015). Copper‐induced root growth inhibition of Allium cepa var. agrogarum L. involves disturbances in cell division and DNA damage. Environ Toxicol Chem, 34 (5), 1045-1055. https://doi.org:10.1002/etc.2884
Rossato, A., Oliveira, P. S., & Sagrillo, M. R. (2021). Microalgas como aditivos de descontaminação de ambientes aquáticos: uma revisão. Disciplinarum Scientia| Naturais e Tecnológicas, 22 (2), 25-37. https://doi.org/10.37779/nt.v22i2.3624
Saccomori, N. L. (2021). Bioestimulantes à base de extrato de algas marinhas na agricultura: estado da arte e potencial de uso. Monografia, Universidade Federal da Integração Latino-Americana, Foz do Iguaçu, Paraná, Brasil. http://dspace.unila.edu.br/123456789/6162
Safaei, M., Maleki, H., Soleimanpour, H., Norouzy, A., Zahiri, H. S., Vali, H., & Noghabi, K. A. (2019). Development of a novel method for the purification of C-phycocyanin pigment from a local cyanobacterial strain Limnothrix sp. NS01 and evaluation of its anticancer properties. Scientific Reports, 9 (1), 1-16. https://doi.org/10.1038/s41598-019-45905-6
Sayadi, M. H., Rashki, O., & Shahri, E. (2019). Application of modified Spirulina platensis and Chlorella vulgaris powder on the adsorption of heavy metals from aqueous solutions. Journal of Environmental Chemical Engineering, 7, 103169. https://doi.org/10.1016/j.jece.2019.103169
Słoczyńska, K., Powroźnik, B., Pękala, E., & Waszkielewicz, A. M. (2014). Antimutagenic compounds and their possible mechanisms of action. Journal of Appl Genet, 55 (2), 273-285. https://doi.org/10.1007/s13353-014-0198-9
Soares, L. M., Coldebella, P. F., & Frigo, J. P. (2021). Avaliação da qualidade da água de rios brasileiros utilizando células meristemáticas de Allium cepa como bioindicador: uma revisão integrativa. Brazilian Journal of Development, 7, (1), 6983-6999. https://doi.org/10.34117/bjdv7n1-473
Utex. Culture collection of algae at the University of Texas at Austin. https://utex.org/
Zhao, P., Yu, X., Li, J., & Tang, Z. (2014). Enhancing lipid productivity by co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10. Journal of Bioscience and Bioengineering, 118 (1), 72-77. https://doi.org/10.1016/j.jbiosc.2013.12.014.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Inalda Maria de Oliveira Messias; Gerusa Tomaz de Aquino Beltrão; Júlio Brando Messias ; Adriano Gomes Ferreira ; Giselly Thais da Silva Oliveira; Lúcio Arantes de Andrade; Silvana de Fátima Ferreira da Silva Caires; Raquel Pedrosa Bezerra; Daniela Araújo Viana Marques; Ana Lúcia Figueiredo Porto
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.