Evaluation of the mutagenic and antimuutagenic activity of Chlorella vulgaris in a test of Allium cepa

Authors

DOI:

https://doi.org/10.33448/rsd-v11i5.28346

Keywords:

Microalgae; Copper sulfate; Mutagenicity test; DNA damage.

Abstract

The microalgae Chlorella vulgaris is usually commercialized as nutraceutical although it has potential application in the pharmaceutical and cosmetic industries. Therefore, our objective in this research is to evaluate the mutagenic and antimutagenic action of the aqueous extract of C. vulgaris through the Allium cepa assay. Three concentrations of the aqueous extract of C. vulgaris were tested: 0.075, 0.15 and 0.30mg/mL. In the mutagenicity analysis, A. cepa meristematic cells were cultured in the presence of the aqueous extract of C. vulgaris with distilled water as negative control and copper sulfate as positive control. For antimutagenicity, pre-treatment, simple simultaneous and post-treatment protocols were used. 400 cells/treatment were analyzed under optical microscopy (40x). Data were analyzed by ANOVA (one-way) and Tukey tests, considering p<0.05. The aqueous extract of C. vulgaris did not show mutagenicity in any of the three concentrations evaluated. About the antimutagenicity protocols, the harm reduction percentages were 94.7%, 94.1% and 96.2% (pre-treatment); 88.9%, 93.2% and 91.08% (simultaneous simple); and 85.2%, 84.5% and 94.7% (post-treatment) referring to concentrations of 0.075, 0.15 and 0.30 mg/mL, respectively. According to these results, the microalgae C. vulgaris did not show mutagenic action at the tested doses and it reduced genetic damage caused by copper sulfate.

Author Biographies

Gerusa Tomaz de Aquino Beltrão, University of Pernambuco

Assistant Professor of Genetics at the University of Pernambuco

Júlio Brando Messias , University of Pernambuco

Associate Professor at the University of Pernambuco

Adriano Gomes Ferreira , Federal University of Pernambuco

Assistant Professor at the Federal Rural University of Pernambuco

Giselly Thais da Silva Oliveira, University of Pernambuco

Student of Biological Sciences at the University of Pernambuco

Lúcio Arantes de Andrade, University of Pernambuco

Student of Biological Sciences at the University of Pernambuco

Silvana de Fátima Ferreira da Silva Caires, University of Pernambuco

Associate Professor at the University of Pernambuco

Raquel Pedrosa Bezerra, Federal Rural University of Pernambuco

Associate Professor at the Federal Rural University of Pernambuco

Daniela Araújo Viana Marques, University of Pernambuco

Associate Professor at the University of Pernambuco

Ana Lúcia Figueiredo Porto, Federal Rural University of Pernambuco

Full Professor of the Federal Rural University of Pernambuco

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14/04/2022

How to Cite

MESSIAS, I. M. de O.; BELTRÃO, G. T. . de A. .; MESSIAS , J. B.; FERREIRA , A. G. .; OLIVEIRA, G. T. da S. . .; ANDRADE, L. A. de .; CAIRES, S. de F. F. da S.; BEZERRA, R. P.; MARQUES, D. A. V. .; PORTO, A. L. F. Evaluation of the mutagenic and antimuutagenic activity of Chlorella vulgaris in a test of Allium cepa. Research, Society and Development, [S. l.], v. 11, n. 5, p. e49911528346, 2022. DOI: 10.33448/rsd-v11i5.28346. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/28346. Acesso em: 14 nov. 2024.

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Agrarian and Biological Sciences