Traditional, Phytochemical and Biological Studies of Ayapana triplinervis (Vahl) R.M. King & H. Robinson: State of art



Ayapana triplinervis; Biological studies; Phytochemistry; Japana; Traditional use.


Ayapana triplinervis (Vahl) R.M. King & H. Robinson is a plant species native to the Amazon region, belonging to the Asteraceae family, popularly known as Japana, which can be found throughout Brazil and in other Latin American, Caribbean and Asian countries. In folk medicine, there are several claims of medicinal use, this therapeutic potential of A. triplinervis has aroused the interest of many research groups around the world, which seek to elucidate its chemical constitution and evaluate its activities against biological systems, in vitro and in alive. Therefore, the objective of this work is to carry out a survey of all the studies already published of A. triplinervis in relation to its traditional use, chemical constituents and biological activity, in order to contribute to the scientific community that is interested in deepening its knowledge about this species. medicinal plant. A bibliographic survey was carried out, where searches were made in databases from the medicinal plant Ayapana triplinervis (Vahl) R.M. King & H. Robinson and their scientific synonyms published on the website, the databases used in the research were SCOPUS, WEB OF SCIENCE, SCIELO and PUBMED. From this review we can observe chemical compounds identified in the species, as well as potential biological activities related to the use of Japana, being evidenced in the published works the activities: antimicrobial, antiviral, antinociceptive and anti-inflammatory with promising results for the research and development of new drugs.


A Balbach. (1993). As plantas curam. Vida Plena.

Adcock, R. S., Chu, Y.-K., Golden, J. E., & Chung, D.-H. (2017). Evaluation of anti-Zika virus activities of broad-spectrum antivirals and NIH clinical collection compounds using a cell-based, high-throughput screen assay. Antiviral Research, 138, 47–56.

Ahmad, S. S., Erum, S., Khan, S. M., Nawaz, M., & Wahid, A. (2014). Exploring the Medicinal Plants Wealth: A Traditional Medico-Botanical Knowledge of Local Communities in Changa. Middle-East Journal of Scientific Research, 20(12).

Arung, E. T., Kuspradini, H., Kusuma, I. W., Shimizu, K., & Kondo, R. (2012). Validation of Eupatorium triplinerve Vahl Leaves, a Skin Care Herb from East Kalimantan, Using a Melanin Biosynthesis Assay. Journal of Acupuncture and Meridian Studies, 5(2), 87–92.

Balick, M. J., Denevan, W. M., Padoch, C., Prance, G. T., Boom, B. M., Darrell Addison Posey, BaléeW. L., Nepstad, D. C., Schwartzman, S., Alexiades, M. N., Carlson, T. J. S., BrondízioE. S., & Maffi, L. (1984). Advances in economic botany. The New York Botanical Garden.

Bose, P., & Roy, A. (1936). The constitution of ayapanin. Journal of the Indian Chemical Society, 13, 586–587.

Brack, A. (1999). Diccionario enciclopedico de plantas utiles del Perú. Cbc; Centro De Estudios Regionales Andinos "Bartolomé De Las Casas.

Castro, L.P. et al. Úlcera Péptica Gastroduodenal :Tratado de clínica médica. 2. ed. São Paulo: ed. Roca, 2009.

Chatuverdi, R., & Mulchandani, N. (1989). Coumarins from Eupatorium ayapana. Journal Indian Chemical Society, 66, 286–287.

Cheriyan, B., Kadhirvelu, P., Nadipelly, J., Shanmugasundaram, J., Sayeli, V., & Subramanian, V. (2017). Anti-nociceptive Effect of 7-methoxy Coumarin from Eupatorium Triplinerve vahl (Asteraceae). Pharmacogn Mag, 13(49), 81–84.

Cheriyan, B., Viswanathan, S., Jagan, N., Parimala, K., Vijaykumar, S., & Venugopal, V. (2013). Anti-inflammatory activity of alcoholic extract of E. triplinerve (Vahl) and its fractions: possible mechanisms. Indo American Journal of Pharmaceutical Research, 3(9), 7095–7102.

Cruz, L. O., Almeida, M. K. C., & Dolabela, M. F. (2020). Contributions of complementary therapies and other strategies to the treatment of schizophrenia: an integrative review. Research, Society and Development, 9(11), e76791110376.

DAS, A., & BONDYA, S. (2015). INDIGENOUS HERBAL HEALERS OF DUMKA DISTRICT: REPOSITORIES OF ETHNO-MEDICINES. International Journal Indegenous for Exchange of Knowledge, 2(1), 25–33.

Deepthy, R., & Remashree, A. (2014). Ethano Botanical Studies on Medicinal Plants Used For Skin Diseases in Malabar Region of Kerala Deepthy R and Remashree AB. International Journal of Herbal Medicine, 2(1), 92–99.

Defilipps, R. A., Maina, S. L., Crepin, J., & National Museum Of Natural History (U.S.). Department Of Botany. (2004). Medicinal plants of the Guianas (Guyana, Surinam, French Guiana). Dept. Of Botany, National Museum Of Natural History, Smithsonian Institution.

Di Stasi, C. (1989). Plantas medicinais na Amazônia. Editora Unesp.

Elisabetsky, E., & Castilhos, Z. C. (1990). Plants Used as Analgesics by Amazonian Caboclos as a Basis for Selecting Plants for Investigation. International Journal of Crude Drug Research, 28(4), 309–320.

Facknath, S., & Lalljee, B. (2008). Study of various extracts of Ayapana triplinervis for their potential in controlling three insect pests of horticultural crops. Tropicultura, 26(2), 119–124.

Frederico Moreira. (1985). As plantas que curam : cuide da sua saude atraves da natureza. Hemus.

Garg, S., & Nakhare, S. (1993). Studies on the essential oil from the flowers of Eupatorium triplinerve. Índian Perfumer, 37, 318–323.

Garg, S., & Nigam, S. (1970). Chemical examination of the essential oil from the leaves of Eupatorium triplinerve (Vahl). Flavour Industry, 1, 469–472.

Gauvin-Bialecki, A., & Marodon, C. (2009). Essential oil of Ayapana triplinervis from Reunion Island: A good natural source of thymohydroquinone dimethyl ether. Biochemical Systematics and Ecology, 36(11), 853–858.

Ghosh, A. (2003). Herbal folk remedies of Bankura and Medinipur districts, W.B. Indian Journal of Traditional Knowledge, 2(4).

Groover, R., & Moore, J. (1962). Toximetric Studies of Fungicides against the Brown Rot Organisms, Sclerotinia fructicola and S. laxa. Phytopathology, 52, 876–879.

Guillermo Arévalo V. (1994). Las plantas medicinales y su beneficio en la salud Shipibo-Conibo. Edición Aidesep.

Gupta, D., Charles, R., & Garg, S. N. (2004). Chemical Composition of the Essential Oil from the Leaves ofEupatorium triplinerveVahl. Journal of Essential Oil Research, 16(5), 473–475.

Haddad, J. G., Picard, M., Bénard, S., Desvignes, C., Desprès, P., Diotel, N., & El Kalamouni, C. (2019). Ayapana triplinervis Essential Oil and Its Main Component Thymohydroquinone Dimethyl Ether Inhibit Zika Virus at Doses Devoid of Toxicity in Zebrafish. Molecules, 24(19), 3447.

Home. (2022).

Jonville, M. C., Kodja, H., Humeau, L., Fournel, J., De Mol, P., Cao, M., Angenot, L., & Frédérich, M. (2008). Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity. Journal of Ethnopharmacology, 120(3), 382–386.

Kathirvelu, P., Cheriyan, B. V., Jagan, N., Viswanathan, S., Kumar, S. V., & Kamalakannan, P. (2019). Gastroprotective and Antioxidant effect of Petroleum ether Extract of Eupatorium triplinerve Vahl. Research Journal of Pharmacy and Technology, 12(11), 5163.

Lans, C. (2007a). Comparison of plants used for skin and stomach problems in Trinidad and Tobago with Asian ethnomedicine. Journal of Ethnobiology and Ethnomedicine, 3(1).

Lans, C. (2007b). Ethnomedicines used in Trinidad and Tobago for reproductive problems. Journal of Ethnobiology and Ethnomedicine, 3(1).

Loguercio, A. P., Battistin, A., Vargas, A. C. de, Henzel, A., & Witt, N. M. (2005). Atividade antibacteriana de extrato hidro-alcoólico de folhas de jambolão (Syzygium cumini (L.) Skells). Ciência Rural, 35(2), 371–376.

MAG (Org ) Jardim. (2009). Diversidade biológica das áreas deproteção ambiental Ilhas do Combu e Algodoal-Maiandeua, Pará, Brasil. Belém, Pa: Mpeg; Mct; Cnpq.

Mahomoodally, M. F., & Muthoorah, L. D. (2014). An ethnopharmacological survey of natural remedies used by the Chinese community in Mauritius. Asian Pacific Journal of Tropical Biomedicine, 4, S387–S399.

Mahomoodally, M. F., & Sreekeesoon, D. P. (2014). A Quantitative Ethnopharmacological Documentation of Natural Pharmacological Agents Used by Pediatric Patients in Mauritius. BioMed Research International, 2014, 1–14.

Maity, P., Bepari, M., & Choudhury, S. M. (2015). Antimitotic, apoptotic and antineoplastic potential of leaf extract of Eupatorium ayapana. International Journal of Phytomedicine, 7(1), 69–77.

Mala, J. G. S., Zoghbi, M. G. B., da Silva, M. H. L., & Andrade, E. H. A. (1999). Essential Oils ofEupatorium triplinerveVahl andE. paniculatumPoepp. et Endl. Journal of Essential Oil Research, 11(5), 541–544.

Manfred, L. (1977). Siete mil recetas botanicas a base de mil trescientas plantas medicinales. Editorial Kier.

Manoel Pio Corrêa. (1984). Dicionário das plantas úteis do Brasil e das exóticas cultivadas. Imprensa Nacional.

Matos Lopes, T. R., de Oliveira, F. R., Malheiros, F. F., de Andrade, M. A., Monteiro, M. C., & Baetas Gonçalves, A. C. (2014). Antimicrobial bioassay-guided fractionation of a methanol extract ofEupatorium triplinerve. Pharmaceutical Biology, 53(6), 897–903.

Melo, A. S., Monteiro, M. C., da Silva, J. B., de Oliveira, F. R., Vieira, J. L. F., de Andrade, M. A., Baetas, A. C., Sakai, J. T., Ferreira, F. A., Cunha Sousa, P. J. da, & Maia, C. do S. F. (2013). Antinociceptive, neurobehavioral and antioxidant effects of Eupatorium triplinerve Vahl on rats. Journal of Ethnopharmacology, 147(2), 293–301.

Mondal, Prabir; Ratnabali Maity; Chhanda Mallick, Efeito espermicida in vitro de folhas de Thevetia Peruviana em espermatozóides humanos , Andrologia , 10.1111/and.14323, 54, 2, (2021) .

Mors, W. B., Carlos Toledo Rizzini, Nuno Alvares Pereira, & Defilipps, R. A. (2000). Medicinal plants of Brazil. Reference Publications.

Natarajan, R., & Natarajan, M. (1979). Phytochemical investigation of Eupatorium ayapana. Journal of Research Indian Medicine, Yoga and Homeopathy, 14, 155–156.

Nunkoo, D. H., & Mahomoodally, M. F. (2012). Ethnopharmacological survey of native remedies commonly used against infectious diseases in the tropical island of Mauritius. Journal of Ethnopharmacology, 143(2), 548–564.

Paixão, T. (2016). Estudo fitoquímico e avaliação do efeito antinociceptivo de uma fração rica em cumarina de Ayapana triplinervis (Asteraceae) [MSc Thesis].

Palheta, I., Tavares-Martins, A. C., & Lucas, F. C. (2017). Ethnobotanical study of medicinal plants in urban home gardens in the city of Abaetetuba, Pará state, Brazil. Boletín Latinoamericano Y Del Caribe de Plantas Medicinales Y Aromáticas, 16(3), 206–262.

Parimala, K., Cheriyan, B., & Viswanathan, S. (2012). Antinociceptive and antiinflammatory activity of petroleum-ether extract of Eupatorium triplinerve Vahl. International Journal of Life Science and Pharma Research, 2(3), 12–18.

Pattanayak, S., Mandal, T., & Andyopadhyay, S. (2016). Ethno-gynecological study on the medicinal plants traditionally used insouthern districts of West Bengal, India. Indian Journal of Traditional Knowledge, 15(3).

Paul Le Cointe. (1947). Árvores e plantas úteis : (indígenas e aclimadas) : nomes vernáculos e nomes vulgares, classificação botânica, habitat, principais aplicações e propriedades : Amazônia brasileira III. Companhia Editora Nacional.

Rahman, A., & Jamila, M. (2016). Village of Chapai Nawabganj District, Bangladesh with Emphasis on Medicinal Plants. Research in Plant Sciences, 4(1), 1–9.

Ricardo, L. M., Paula-Souza, J. de, Andrade, A., & Brandão, M. G. L. (2017). Plants from the Brazilian Traditional Medicine: species from the books of the Polish physician Piotr Czerniewicz (Pedro Luiz Napoleão Chernoviz, 1812–1881). Revista Brasileira de Farmacognosia, 27(3), 388–400.

Samoisy, A. K., & Mahomoodally, F. (2015). Ethnopharmacological analysis of medicinal plants used against non-communicable diseases in Rodrigues Island, Indian Ocean. Journal of Ethnopharmacology, 173, 20–38.

Samoisy, A. K., & Mahomoodally, F. (2016). Ethnopharmacological appraisal of culturally important medicinal plants and polyherbal formulas used against communicable diseases in Rodrigues Island. Journal of Ethnopharmacology, 194, 803–818.

Selvamangai, G., & Bhaskar, A. (2012). GC–MS analysis of phytocomponents in the methanolic extract of Eupatorium triplinerve. Asian Pacific Journal of Tropical Biomedicine, 2(3), S1329–S1332.

Sharma, C., & Chetri, A. (2017). DOCUMENTATION OF TRADITIONAL MEDICINAL PLANTS USED BY GARO TRIBE IN DIMORIA TRIBAL BELT OF ASSAM. International Journal of Innovative in Science and Engineering, 3(3), 299–306.

Singh, A. (2012). Phytomedicinal investigation for antimicrobials based on chemical and biological properties of herbal medicines: An overview. Novel Science International Journal of Pharmaceutical Science, 1(7), 405–424.

Soplin, S. P., Millones, E. A., Campos, J. L. A., Deza, L. G., Ríos, E. J., Sánchez, I. B., & Benítez, M. R. (1996). Perú: Informe Nacional para la Conferencia Técnica Internacional de la FAO sobre los Recursos Fitogenéticos (Leipzig, 1996). Internal report.

Sreekeesoon, D. P., & Mahomoodally, M. F. (2014). Ethnopharmacological analysis of medicinal plants and animals used in the treatment and management of pain in Mauritius. Journal of Ethnopharmacology, 157, 181–200.

Sugumar, N., Karthikeyan, S., & Gowdhami, T. (2015). Chemical composition and antimicrobial activity of essential oil from Eupatorium triplinerve Vahl. aerial parts. International Letters of Natural Sciences, 4, 14–21.

Taïlé, J., Patché, J., Veeren, B., & Gonthier, M.-P. (2021). Condição hiperglicêmica causa alterações pró-inflamatórias e de permeabilidade associadas ao recrutamento de monócitos e vias desreguladas de NFκB/PPARγ em células endoteliais cerebrais: evidências de absorção de polifenóis e efeito protetor. Jornal Internacional de Ciências Moleculares, 22 (3), 1385. MDPI AG. Recuperado de

Tareau, M. A., Palisse, M., & Odonne, G. (2017). As vivid as a weed… Medicinal and cosmetic plant uses amongst the urban youth in French Guiana. Journal of Ethnopharmacology, 203, 200–213.

Taylor, L. (2006). Technical Data Report for Ayapana (Ayapana triplinervis).

Teodoro Agapito F, & Sung, I. (2003). Fitomedicina : 1100 plantas medicinales. Editorial Isabel.

Trang, N., Dung, N., Phuong, L., Wanner, M., & Koomen, G. (1992). The 13C-NMR spectroscopy of ayapin isolated from Eupatorium ayapana Vent. from Vietnam, Tap Chi Ho´ a Hoc. Journal of Chemistry, 30, 62–63.

Trang, N., Wanner, M., Phuong, L. V., Koomen, G., & Dung, N. (1993). Thymoquinone fromEupatorium ayapana. Planta Medica, 59(01), 99–99.

Uddin, M. Z., Hassan, M. A., & Sultana, M. (1970). Ethnobotanical survey of medicinal plants in Phulbari Upazila of Dinajpur District, Bangladesh. Bangladesh Journal of Plant Taxonomy, 13(1), 63–68.

Unnikrishnan, P. K., Varughese, T., Sreedhar, S., Balan, N., Balachandran, I., & Rema Shree, A. B. (2014). Study onEupatorium triplinerveVahl from South India, A Rich Source for Thymohydroquinone dimethylether and its Antimicrobial Activity. Journal of Essential Oil Bearing Plants, 17(4), 652–657.

Vásquez, S. P. F., Mendonça, M. S. de, & Noda, S. do N. (2014). Etnobotânica de plantas medicinais em comunidades ribeirinhas do Município de Manacapuru, Amazonas, Brasil. Acta Amazonica, 44(4), 457–472.

Vieira, L. S. (1992). Fitoterapia da Amazonia: manual das plantas medicinais: a farmacia de Deus. Editora Agronomica Ceres.



How to Cite

MORAES, C. S. P. de .; PAIXÃO, T. P. da .; PANTOJA, L. V. P. da S.; OKABE, D. H. .; TRINDADE, S. S. A. .; VIEIRA, J. G. de P. .; GONÇALVES, A. C. B. .; ANDRADE, M. A. de . Traditional, Phytochemical and Biological Studies of Ayapana triplinervis (Vahl) R.M. King & H. Robinson: State of art. Research, Society and Development, [S. l.], v. 11, n. 6, p. e6411628648, 2022. Disponível em: Acesso em: 29 may. 2022.



Health Sciences