Influence of Eucalyptus spp. essential oils and tannins on herbivory




Astringency of plants; Phenolic compounds; Protection against herbivory.


Eucalyptus (family Myrtaceae), a native plant from Australia, is a genus cultivated due to its oil, gum, pulp and timber used worldwide for different commercial applications. It is a plant with significant economic importance and possibly its cultivation, in Brazil and other countries, has reduced the exploitation of native trees. The secondary metabolism of Eucalyptus, as usually found in plants, produces essential oils and tannins, which may interfere in the consumption of plants by wild animals. The goal of the present work was to investigate the influence of essential oil and tannins concentration on the herbivory of seven Eucalyptus taxa. Essential oils composition for all species investigated were characterized by CG MS HS-Trap analysis. The lowest hebivory rate were associated to Urocam, Grancam and Eucalyptus benthamii. Those taxa were associated with the presence of mix oil compounds, α/β-Pinene, Pinocarveol, α-Terpineol and Tannins. Tannins concentrations and essential oil are an important factor that contributes to the plant protection against herbivory.


Amazonas, N. T., Forrester, D. I., Silva, C. C., Almeida, D. R. A., Rodrigues, R. R., & Brancalion, P. H. S. (2018). High diversity mixed plantations of Eucalyptus and native trees: An interface between production and restoration for the tropics. Forest Ecology and Management, 417(November 2017), 247–256.

Bailey, J. K., Wooley, S. C., Lindroth, R. L., & Whitham, T. G. (2006). Importance of species interactions to community heritability: A genetic basis to trophic-level interactions. Ecology Letters, 9(1), 78–85.

Baldi, A., Bruschi, P., Campeggi, S., Egea, T., Rivera, D., Obón, C., & Lenzi, A. (2022). The Renaissance of Wild Food Plants: Insights from Tuscany (Italy). Foods, 11(3).

Batish, D. R., Singh, H. P., Kohli, R. K., & Kaur, S. (2008). Eucalyptus essential oil as a natural pesticide. Forest Ecology and Management, 256(12), 2166–2174.

Batista, T. S., Estevão, C. D., De Lima, D. C., & Salvio, G. M. M. (2021). Mammals in atlantic forest remnants of barbacena, minas gerais mamíferos em remanescentes florestais de mata atlântica, barbacena, minas gerais. Ciencia Animal Brasileira, 22.

Canale, G. R., Guidorizzi, C. E., Kierulff, M. C. M., & Gatto, C. A. F. R. (2009). First record of tool use by wild populations of the yellow-breasted capuchin monkey (Cebus xanthosternos) and new records for the bearded capuchin (cebus libidinosus). American Journal of Primatology, 71(5), 366–372.

Carrara, L. A., Faria, L. D. P., Do Amaral, F. Q., & Rodrigues, M. (2007). Dormit??rios do papagaio-verdadeiro Amazona aestiva e do papagaio-galego Salvatoria xanthops em plantio comercial de eucalipto. Revista Brasileira de Ornitologia, 15(1), 135–138.

Cheng, Y., Savits, J. R., & Watrelot, A. A. (2022). Effect of the Application Time of Accentuated Cut Edges (ACE) on Marquette Wine Phenolic Compounds. Molecules, 27(2).

Chouvelon, T., Gilbert, L., Caurant, F., Méndez‐Fernandez, P., Bustamante, P., Brault‐Favrou, M., & Spitz, J. (2022). Nutritional grouping of marine forage species reveals contrasted exposure of high trophic levels to essential micro‐nutrients. Oikos, February, 1–22.

Domingues, R. M. A., Sousa, G. D. A., Silva, C. M., Freire, C. S. R., Silvestre, A. J. D., & Neto, C. P. (2011). High value triterpenic compounds from the outer barks of several Eucalyptus species cultivated in Brazil and in Portugal. Industrial Crops and Products, 33(1), 158–164.

Dutt, D., & Tyagi, C. H. (2011). Comparison of various eucalyptus species for their morphological, chemical, pulp and paper making characteristics. Indian Journal of Chemical Technology, 18(2), 145–151.

Felton, A. M., Felton, A., Lindenmayer, D. B., & Foley, W. J. (2009). Nutritional goals of wild primates. In Functional Ecology. 23(1), 70–78).

Freitas, C. H. de, Setz, E. Z. F., Araújo, A. R. B., & Gobbi, N. (2008). Agricultural crops in the diet of bearded capuchin monkeys, Cebus libidinosus Spix (Primates: Cebidae), in forest fragments in southeast Brazil. Revista Brasileira de Zoologia, 25(1), 32–39.

Galetti, M. (1993). Diet of the Scaly-Headed Parrot (Pionus maximiliani) in a Semideciuous Forest in Southeatern Brazil. Biotropica, 25(4), 419–425.

Harris, R. (2003). Eucalyptus. The genus Eucalyptus. International Journal of Aromatherapy, 13(2–3), 152–153.

Hohenlohe, P. A., Funk, W. C., & Rajora, O. P. (2021). Population genomics for wildlife conservation and management. Molecular Ecology, 30(1), 62–82.

Jacq, C., Da, R., Saad, G., Toghlobi, S. El, Arantes, R. A., Knudsen, B. G., Aurélio, M., Pereira, A., Carvalho, R. G. De, Ribeiro, R., Ferraz, N., Sandro, F., & Rodrigues, M. (2022). International Journal of Health Management Review, 8(1).

Kullan, A. R., van Dyk, M. M., Hefer, C. a, Jones, N., Kanzler, A., & Myburg, A. a. (2012). Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of Eucalyptus grandis and E. urophylla. BMC Genetics, 13(1), 60.

Guia do Eucalipto: oportunidades para um desenvolvimento sustentável, Council for Biotechnology information (2008).

Lesschaeve, I., & Noble, A. C. (2005). Polyphenols: factors influencing their sensory properties and their effects on food and beverage preferences. The American Journal of Clinical Nutrition, 81(1 Suppl), 330S-335S.

Mehansho, H., Ann, D. K., Butler, L. G., Rogler, J., & Carlson, D. M. (1987). Induction of proline-rich proteins in hamster salivary glands by isoproterenol treatment and an unusual growth inhibition by tannins. Journal of Biological Chemistry, 262(25), 12344–12350.

Mikich, S. B., & Liebsch, D. (2014). Damage to forest plantations by tufted capuchins (Sapajus nigritus): Too many monkeys or not enough fruits? Forest Ecology and Management, 314, 9–16.

Mithöfer, A., & Boland, W. (2012). Plant Defense Against Herbivores: Chemical Aspects. Annual Review of Plant Biology, 63(1), 431–450.

Mokrzycki, Ł., Sulikowski, B., & Olejniczak, Z. (2009). Properties of desilicated ZSM-5, ZSM-12, MCM-22 and ZSM-12/MCM-41 derivatives in isomerization of α-pinene. Catalysis Letters, 127(3–4), 296–303.

Mossi, a J., Mazutti, M., Paroul, N., Corazza, M. L., Dariva, C., Cansian, R. L., & Oliveira, J. V. (2009). Chemical variation of tannins and triterpenes in Brazilian populations of Maytenus ilicifolia Mart. Ex Reiss. Brazilian Journal of Biology = Revista Brasleira de Biologia, 69(2), 339–345.

Mueller-Harvey, I. (2006). Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture, 86(13), 2010–2037.

Neiva, D., Fernandes, L., Araújo, S., Lourenço, A., Gominho, J., Simões, R., & Pereira, H. (2015). Chemical composition and kraft pulping potential of 12 eucalypt species. Industrial Crops and Products, 66, 30–30.

Oliveira, N. De, Carolina, A., Figueiredo, F., Doril, G., Cordeiro, F. R., Cristina, G., Silva, C., Marques, R. D. O., Silva, M., Dalarme, L., Gouvea, I., & Cardoso, M. (2022). Land Use , Land Cover Change and Sustainable Intensification of Agriculture and Livestock in the Amazon and the Atlantic Forest in Brazil. Sustainability, 1–23. su14052563

Padmanaban, P. B. S., Rosenkranz, M., Zhu, P., Kaling, M., Schmidt, A., Schmitt-Kopplin, P., Polle, A., & Schnitzler, J. P. (2022). Mycorrhiza-Tree-Herbivore Interactions: Alterations in Poplar Metabolome and Volatilome. Metabolites, 12(2).

Parys, W., Dołowy, M., & Pyka-Pająk, A. (2022). Significance of Chromatographic Techniques in Pharmaceutical Analysis. Processes, 10(1).

Prache, S., Adamiec, C., Astruc, T., Baéza-Campone, E., Bouillot, P. E., Clinquart, A., Feidt, C., Fourat, E., Gautron, J., Girard, A., Guillier, L., Kesse-Guyot, E., Lebret, B., Lefèvre, F., Le Perchec, S., Martin, B., Mirade, P. S., Pierre, F., Raulet, M., & Santé-Lhoutellier, V. (2022). Review: Quality of animal-source foods. Animal, 16, 100376.

Rafferty, C., & Lamont, B. B. (2021). Plant tannins and essential oils have an additive deterrent effect on diet choice by kangaroos. Forests, 12(12).

Rider, C. V. (2016). TOX- 81_ α-Pinene Administered by Inhalation to F344_N Rats and B6C3F1_N Mice.pdf (pp. 1–58). National Report Series.

Sartoratto, A., Machado, A. L. M., Delarmelina, C., Figueira, G. M., Duarte, M. C. T., & Rehder, V. L. G. (2004). Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology, 35(4), 275–280.

Silva, A. C. R. da, Lopes, P. M., Azevedo, M. M. B. de, Costa, D. C. M., Alviano, C. S., & Alviano, D. S. (2012). Biological Activities of a-Pinene and β-Pinene Enantiomers. Molecules, 17(12), 6305–6316.

Trugillho, P. F., Caixeta, R. P., Lima, J. T., & Mendes, L. M. (1997). Avaliação Do Conteúdo Em Taninos Condensados De Algumas Espécies Típicas Do Cerrado Mineiro. Cerne, Lavras, 3(1), 1–13.

Turek, C., & Stintzing, F. C. (2013). Stability of essential oils: A review. In Comprehensive Reviews in Food Science and Food Safety (Vol. 12, Issue 1, pp. 40–53).




How to Cite

MÜLLER, E. S.; HÖHN, C.; ALMEIDA, M. O. P. de .; PATUSSI, P. .; CALISTO, J. F. F. .; FLOSS, P.; OLIVEIRA, A. D. de .; ALBENY-SIMÕES, D.; SOUZA, R. R. de .; OLIVEIRA, J. V. .; MAGRO, J. D. . Influence of Eucalyptus spp. essential oils and tannins on herbivory. Research, Society and Development, [S. l.], v. 11, n. 6, p. e14611628903, 2022. DOI: 10.33448/rsd-v11i6.28903. Disponível em: Acesso em: 22 may. 2022.



Agrarian and Biological Sciences