In vitro evaluation of Álcool desidrogenase kinetics in Ziziphus joazeiro fruits to alleviate the harmful effects of alcohol
DOI:
https://doi.org/10.33448/rsd-v9i4.2900Keywords:
Acetaldehyde; Alcohol dehydrogenase; Aldehyde dehydrogenase.Abstract
Ziziphus joazeiro is endemic to the brazilian Caatinga and its fruits can be used as a food supplement by accelerating the ethanol metabolism in the body and reducing the alcohol harmful effects due to high alcohol dehydrogenase (ADH) activity. The objective was to determine the kinetics of ADH activity, in different incubation times, of Z. joazeiro mature fruits as a food supplement. Ziziphus joazeiro fruits, at the fourth maturation stage, were incubated for 0 (no incubation), 3, 6, 12, 24 and 48 hours at room temperature. The ADH activity was determined. ADH activity was higher in fruits incubated for 0 and 3 h. The ADH activity was higher in the early incubation times, probably due to the greater availability of NAD+, which after being reduced to NADH delayed regeneration. Without the cofactor in the oxidized form, the enzymatic activity decreases. Ziziphus joazeiro fruit has the potential to be used as a food supplement accelerating the alcohol metabolism and reducing the harmful effects.
References
Bornscheuer, U.T., Huisman, G.W., Kazlauskas, R.J., Lutz S, Moore JC, Robins K. (2012). Engineering the third wave of biocatalysis. Nature, 485: 185 – 194.
Burton, R., Sheron, N. (2018). No level of alcohol consumption improves health. The Lancet, 392: 987-988.
Cederbaum, A.I. (2012). Alcohol metabolism. Clinical Liver Disease, 16: 667 – 685.
Espino-Diaz, M., Sepulveda, D.R., González-Aguilar, G., Olivas, G.I. (2016). Biochemistry of apple aroma: A review. Food Technology and Biotechnology, 54: 375 – 394.
Garcia, S.M.N. (2016). Risk of head and neck cancer and the alcohol dehydrogenase 3 genotype. Alcohol and Alcoholism, 45: 6 – 12.
Höfler, G.T., Fernández-Fueyo, E., Pesic, M., Younes, S.H., Choi, E.G., Kim, Y.H., Urlacher, V.B., Arends, I., Hollmann, F. (2018). A photoenzymatic NADH regeneration system. ChemBioChem, 19: 2344 – 2347.
Kiela, P.R., Ghishan, F.K. (2016). Physiology of intestinal absorption and secretion. Best Practice & Research: Clinical Rheumatology, 30: 145 – 159.
Knaus, T., Mutti, F.G. (2017). Biocatalytic hydrogen-borrowing cascades. Chimica Oggi, 35: e11.
Lee, H.S., Isse, T., Kawamoto, T., Baik, H.W., Park, J.Y., Yang, M. (2013). Effect of Korean pear (Pyrus pyrifolia cv. Shingo) juice on hangover severity following alcohol consumption. Food and Chemical Toxicology, 58: 101 – 106.
León-Sánchez, F.D., Pelayo-Zaldívar, C., Rivera-Cabrera, F., Ponce-Valadez, M., Ávila-Alejandre, X., Fernández, F.J., Escalona-Buendía, H.B., Pérez-Flores, L.J. (2009). Effect of refrigerated storage on aroma and alcohol dehydrogenase activity in tomato fruit. Postharvest Biology and Technology, 54: 93 – 100.
Pesis, E. (2005). The role of the anaerobic metabolites, acetaldehyde and ethanol, in fruit ripening, enhancement of fruit quality and fruit deterioration. Postharvest Biology and Technology, 37: 1 – 19.
Ramchandani V.A., Kwo, P.Y., Li, T.K. (2001). Effect of food and food composition on alcohol elimination rates in healthy men and women. Journal of Pharmacological Sciences, 41: 1345 – 1350.
Ramchandani, V.A., Bosron, W.F., Li, T.K. (2001). Research advances in ethanol metabolism. Pathologie Biologie, 49: 676 – 682.
Scoccianti, C., Lauby-Secretan, B., Bello, P.Y., Chajes, V., Romieu, I. (2014). Female breast cancer and alcohol consumption: a review of the literature. American Journal of Preventive Medicine, 46: S16 – S25.
Shabtai, Y., Bendelac, L., Jubran, H., Hirschberg, J., Fainsod, A. (2018). Acetaldehyde inhibits retinoic acid biosynthesis to mediate alcohol teratogenicity. Scientific Reports, 8: e347.
Silva, J.L., Costa, F.B., Nascimento, A.M., Gadelha, T.M., Silva, M.S., Sousa Sátiro, L., Silva, K.G. (2018). Chemical characteristics and bioactive compounds in Juazeiro fruits collected in different maturation stages. Journal of Experimental Agriculture International, 26: 1 – 10.
Singal, A.K., Bailey, S.M. (2018). Cellular abnormalities and emerging biomarkers in alcohol-associated liver disease. Gene Expression, 19: 49 – 60.
UK Department of Health UK Chief Medical Officers' Low Risk Drinking Guidelines. August 2016. Retrieved on March 6, 2019 from: https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/545937/UK_CMOs__report.pdf
Wang, F., Zhang, Y.J., Zhou, Y., Li, Y., Zhou, T., Zheng, J., Zhang, J.J., Li, S., Xu, D.P., Li, H.B. (2016). Effects of beverages on alcohol metabolism: potential health benefits and harmful impacts. International Journal of Molecular Sciences, 17: e354.
Zhang, Y.J., Wang, F., Zhou, Y., Li, Y., Zhou, T., Zheng, J., Zhang, J.J., Li, S., Xu, D.P., Li, H.B. (2016). Effects of 20 Selected Fruits on Ethanol Metabolism: Potential Health Benefits and Harmful Impacts. International Journal of Environmental Research and Public Health, 13: e399.
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