Tolerance and phytoremediation capacity of the Lemna minor in an aqueous medium contaminated by the Amoxicillin

Authors

DOI:

https://doi.org/10.33448/rsd-v11i7.30251

Keywords:

Antibiotic; Duckweeds; Stress; Tolerance.

Abstract

This study aims to evaluate the behavior and the effectiveness of the aquatic plant Lemna minor as a phytoremediation potential about the antibiotic amoxicillin. Experiments were conducted through Central Composite Rotational Design (CCRD) and a kinetic study. The experiments taken by CCRD were performed in culture wells during a contact period of 5 days. The concentration of amoxicillin in the aqueous medium, the quantity of the plant mass, and the solution's pH were studied as independent variables. The dependent variables measured were the remaining amoxicillin in the solution, the plant's tolerance to mortality or fronds cloning and, for stress, chlorophyll-a/chlorophyll-b ratio. A kinetic study determined the rate of antibiotic removal in the aqueous medium. Because the only significant independent variable was the Amoxicillin concentration, the best experimental condition obtained through CCRD was considered the one with the higher level of Amoxicillin removal (92%) - the water medium with the drug concentration at 2.0 mg L-1 and pH 7.0. 5 g of wet mass from L. minor for each 10 mL of solution was chosen since this independent variable was not significant. There was no pH variation in the solution and the plant stress in the kinetics study. However, there was an amoxicillin removal of 80% after the seventh day. It is considered that, under conditions of low antibiotic load, the aquatic plant Lemna minor presents phytoremediation capacity to antibiotic amoxicillin.

References

Abd, I. N., & Mohammed-Ridha, M. J. (2020). Simultaneous adsorption of tetracycline and amoxicillin by cladophora and spirulina algae biomass. Iraqi Journal of Agricultural Sciences, 52(5), 1290–1303.

Alkimin, G. D., Daniel, D., Frankenbach, S., Serôdio, J., Soares, A. M. V. M., Barata, C., & Nunes, B. (2019). Evaluation of pharmaceutical toxic effects of non-standard endpoints on the macrophyte species Lemna minor and Lemna gibba. Science of the Total Environment, 657, 926–937. https://doi.org/10.1016/j.scitotenv.2018.12.002

Driscoll, S. P., Prins, A., Olmos, E., & Kunert, K. J. (2006). Specification of adaxial and abaxial stomata, epidermal structure and photosynthesis to CO2 enrichment in maize leaves. Journal of Experimental Botany, 57(2), 381–390. https://doi.org/10.1093/jxb/erj030

Feng, L., Cheng, Y., Zhang, Y., Li, Z., Yu, Y., Feng, L., Zhang, S., & Xu, L. (2020). Distribution and human health risk assessment of antibiotic residues in large-scale drinking water sources in Chongqing area of the Yangtze River. Environmental Research, 185, 109386. https://doi.org/10.1016/j.envres.2020.109386

Garcia-Rodríguez, A., Matamoros, V., Fontàs, C., & Salvadó, V. (2015). The influence of Lemna sp. and Spirogyra sp. on the removal of pharmaceuticals and endocrine disruptors in treated wastewaters. International Journal of Environmental Science and Technology, 12, 2327–2338. https://doi.org/10.1007/s13762-014-0632-x

Hu, H., Zhou, Q., Li, X., Lou, W., Du, C., Teng, Q., Zhang, D., Liu, H., Zhon, Y., & Yang, C. (2019). Phytoremediation of anaerobically digested swine wastewater contaminated by oxytetracycline via Lemna aequinoctialis: Nutrient removal, growth characteristics and degradation pathways. Bioresource Technology, 291, 121853. https://doi.org/10.1016/j.biortech.2019.121853

Jiao, L., Zhong, N., Zhao, X., Ma, S., Fu, X., & Dong, D. (2020). Recent advances in fiber-optic evanescent wave sensors for monitoring organic and inorganic pollutants in water. Trends in Analytical Chemistry, 127, 115892. https://doi.org/10.1016/j.trac.2020.115892

Kanakaraju, D., Kockler, J., Motti, C. A., Glass, B. D., & Oelgemöller, M. (2015). Applied Catalysis B : Environmental Titanium dioxide / zeolite integrated photocatalytic adsorbents for the degradation of amoxicillin. “Applied Catalysis B, Environmental,” 166–167, 45–55. https://doi.org/10.1016/j.apcatb.2014.11.001

Li, Y., Lian, J., Wu, B., Zou, H., & Tan, S. K. (2020). Phytoremediation of pharmaceutical-contaminated wastewater: Insights into rhizobacterial dynamics related to pollutant degradation mechanisms during plant life cycle. Chemosphere, 253, 126681. https://doi.org/10.1016/j.chemosphere.2020.126681

Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy. Current Protocols in Food Analytical Chemistry, 1(1), F4.3.1-F4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

Lima, M. L., Luís, S., Poggio, L., Aragonés, J. I., Courtier, A., Roig, B., & Calas-Blanchard, C. (2020). The importance of household pharmaceutical products disposal and its risk management: Example from Southwestern Europe. Waste Management, 104, 139–147. https://doi.org/10.1016/j.wasman.2020.01.008

Mateos-Cárdenas, A., Scott, D. T., Seitmaganbetova, G., van, van P., John, O. H., & Marcel A.K., J. (2019). Polyethylene microplastics adhere to Lemna minor (L.), yet have no effects on plant growth or feeding by Gammarus duebeni (Lillj.). Science of the Total Environment, 689, 413–421. https://doi.org/10.1016/j.scitotenv.2019.06.359

Miner, N. A., Mulberry, G. K., Starks, A. N., Powers-Prather, A., Entrup, M., Armstrong, M., & Maida, B. (1995). Identification of possible artifacts in the association of official analytical chemists sporicidal test. Applied and Environmental Microbiology, 61(4), 1658–1660. https://doi.org/10.1128/aem.61.4.1658-1660.1995

Moreira, N. F. F., Orge, C. A., Ribeiro, A. R., Faria, J. L., Nunes, O. C., Pereira, M. F. R., & Silva, A. M. T. (2015). Fast mineralization and detoxification of amoxicillin and diclofenac by photocatalytic ozonation and application to an urban wastewater. Water Research, 15(87), 87–96. https://doi.org/10.1016/j.watres.2015.08.059

Nunes, B., Veiga, V., Frankenbach, S., Serôdio, J., & Pinto, G. (2019). Evaluation of physiological changes induced by the fluoroquinolone antibiotic ciprofloxacin in the freshwater macrophyte species Lemna minor and Lemna gibba. Environmental Toxicology and Pharmacology, 72(January), 103242. https://doi.org/10.1016/j.etap.2019.103242

Reinhold, D., Vishwanathan, S., Park, J. J., Oh, D., & Saunders, F. M. (2010). Assessment of plant-driven removal of emerging organic pollutants by duckweed. Chemosphere, 80(7), 687–692. https://doi.org/10.1016/j.chemosphere.2010.05.045

Sasmaz, M., Öbek, E., & Sasmaz, A. (2019). Bioaccumulation of cadmium and thallium in Pb-Zn tailing waste water by Lemna minor and Lemna gibba. Applied Geochemistry, 100(August 2018), 287–292. https://doi.org/10.1016/j.apgeochem.2018.12.011

Shakak, M., Rezaee, R., Maleki, A., Jafari, A., Safari, M., Shahmoradi, B., Daraei, H., & Lee, S.-M. (2020). Synthesis and characterization of nanocomposite ultrafiltration membrane (PSF/PVP/SiO2) and performance evaluation for the removal of amoxicillin from aqueous solutions. Environmental Technology & Innovation, 17, 100529. https://doi.org/10.1016/j.eti.2019.100529

Shi, W., Wang, L., Roussea, D. P. L., & Lens, P. N. L. (2010). Removal of estrone, 17alpha-ethinylestradiol, and 17beta-estradiol in algae and duckweed-based wastewater treatment systems. Environmental Science and Pollution Research, 17(4), 824–833. https://doi.org/10.1007/s11356-010-0301-7

Silva, D. M., Lizieri, C., & Oliveira Júnior, E. S. (2021). Plantas aquáticas em ecotecnologias: perspectivas para fitorremediação de ferro e manganês. Research, Society and Development, 10(3), e29510313320. https://doi.org/10.33448/rsd-v10i3.13320

Silva, J. F. da. (2012). Prospecção de plantas fitorremediadoras em solos contaminados por metais pesados. Universidade Federal do Amazonas.

Steet, J. A., & Tong, C. H. (2006). Degradation kinetics of green color and chlorophylls in peas by colorimetry and HPLC. Journal of Food Science, 61(5), 924–928. https://doi.org/10.1111/j.1365-2621.1996.tb10903.x

Streit, N. M., Canterle, L. P., Canto, M. W. do, & Hecktheuer, L. H. H. (2005). As clorofilas. Ciência Rural, 35(3), 748–755. https://doi.org/10.1590/s0103-84782005000300043

Taiz, L., & Zeiger, E. (2004). Fisiologia Vegetal (Artmed (ed.); 3a ed.).

Ul-Ain, N., Anis, I., Ahmed, F., Shah, M. R., Parveen, S., Faizi, S., & Ahmed, S. (2018). Colorimetric detection of amoxicillin based on querecetagetin coated silver nanoparticles. Sensors and Actuators B: Chemical, 265, 617–624. https://doi.org/10.1016/j.snb.2018.03.079

Wang, Y., Chen, C., Zhou, D., Xiong, H., Zhou, Y., Dong, S., & Rittmann, B. E. (2019). Chemosphere Eliminating partial-transformation products and mitigating residual toxicity of amoxicillin through intimately coupled photocatalysis and biodegradation. Chemosphere, 237, 124491. https://doi.org/10.1016/j.chemosphere.2019.124491

Xiao, F., Feng, L.-J., Sun, X.-D., Wang, Y., Wang, Z.-W., Zhu, F.-P., & Yuan, X.-Z. (2022). Do Polystyrene Nanoplastics Have Similar Effects on Duckweed ( Lemna minor L.) at Environmentally Relevant and Observed-Effect Concentrations? . Environmental Science & Technology, 56(7), 4071–4079. https://doi.org/10.1021/acs.est.1c06595

Yahia, B., Faouzi, S., Ahmed, C., & Mohamed, T. (2022). A new hybrid process for Amoxicillin elimination by combination of adsorption and photocatalysis on ( CuO / AC ) under solar irradiation. Journal of Molecular Structure, 1261, 132769. https://doi.org/10.1016/j.molstruc.2022.132769

Zhang, Y., Wang, J., Lu, J., & Wu, J. (2020). Antibiotic resistance genes might serve as new indicators for wastewater contamination of coastal waters: Spatial distribution and source apportionment of antibiotic resistance genes in a coastal bay. Ecological Indicators, 114, 106299. https://doi.org/10.1016/j.ecolind.2020.106299

Zhao, F., Chen, L., Yen, H., Li, G., Sun, L., & Yang, L. (2020). An innovative modeling approach of linking land use patterns with soil antibiotic contamination in peri-urban areas. Environment International, 134, 105327. https://doi.org/10.1016/j.envint.2019.105327

Zhao, F., Yan, L., Che, L., Xiang, Q., Li, S., Sun, L., Yu, X., & Fang, L. (2019). Soil contamination with antibiotics in a typical peri-urban area in eastern China: Seasonal variation, risk assessment, and microbial responses. Journal of Environmental Sciences, 79, 200–212. https://doi.org/10.1016/j.jes.2018.11.024

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Published

31/05/2022

How to Cite

CERBARO, K. A. .; ROCHA, R. D. C. da . Tolerance and phytoremediation capacity of the Lemna minor in an aqueous medium contaminated by the Amoxicillin. Research, Society and Development, [S. l.], v. 11, n. 7, p. e45711730251, 2022. DOI: 10.33448/rsd-v11i7.30251. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30251. Acesso em: 22 dec. 2024.

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Section

Engineerings