Chemical evaluation and biological activity of bioactive compounds from Campomanesia xanthocarpa Berg
DOI:
https://doi.org/10.33448/rsd-v11i9.31561Keywords:
Guabiroba; Functional Foods; Anti-inflammation; Anticancer.Abstract
Campomanesia xanthocarpa Berg. (Myrtaceae), also known as “guabiroba”, is widely distributed in the South and Southern regions of Brazil and has been used by local families as folk medicine to treat several diseases. This study aimed to evaluate the bioactive compounds of guabiroba fruits and leaves, such as flavonoids, carotenoids, chlorophyll, total phenolics, tannins, and coumarins. The biological effect of different parts of C. xanthocarpa (leaf and pulp) was also investigated for the immunomodulatory activity on RAW 264.7 macrophages, and anti-cancer effects on H295R adrenocortical cells under different concentrations and extraction protocols. Extracts from guabiroba leaves and pulp using 70% ethanol and n-hexane as a solvent by magnetic stirring, as well as ethanolic extracts from leaves and pulp obtained by heat reflux extraction were produced. Most of the bioactive compounds were found in the leaves rather than the fruit pulp. The in vitro assays showed that the leaf extraction with hexane affected the viability assay in the adrenocortical cell line H295R, while the pulp extracted with hexane activated the macrophage cell line RAW 264.7. Therefore, C. xanthocarpa leaves and pulp have proven to affect macrophage and adrenocortical cell lines in a dose-dependent manner.
References
Alves, A. M., Alves, M. S. O., Fernandes, T. de O., Naves, R. V., & Naves, M. M. V. (2013). Physical and chemical characterization, total phenolics and antioxidant activity of the gabiroba pulp and residue. Revista Brasileira de Fruticultura. 35(3), 837-844. https://doi.org/10.1590/S0100-29452013000300021
Amaral, S. da C., Barbieri, S. F., Ruthes, A. C., Bark, J. M., Brochado Winnischofer, S. M., & Silveira, J. L. M. (2019). Cytotoxic effect of crude and purified pectins from Campomanesia xanthocarpa Berg on human glioblastoma cells. Carbohydrate Polymers, 224(August), 115140. https://doi.org/10.1016/j.carbpol.2019.115140
Annadurai, G., Masilla, B., Jothiramshekar, S., Palanisami, E., Puthiyapurayil, S., & Parida, A. (2012). Antimicrobial, antioxidant, anticancer activities of Syzygium caryophyllatum (L.) Alston. International Journal of Green Pharmacy. 6, 285-288. https://doi.org/10.4103/0973-8258.108210
Barnes, P. J. (1994). Cytokines as mediators of chronic asthma. American Journal of Respiratory and Critical Care Medicine. 150, S42-49. https://doi.org/10.1164/ajrccm/150.5_pt_2.s42
Batista, Â. G., da Silva, J. K., Betim Cazarin, C. B., Biasoto, A. C. T., Sawaya, A. C. H. F., Prado, M. A., & Maróstica Júnior, M. R. (2017). Red-jambo (Syzygium malaccense): Bioactive compounds in fruits and leaves. LWT - Food Science and Technology. 76(B), 284-291. https://doi.org/10.1016/j.lwt.2016.05.013
Bowdish, D. M. E., Loffredo, M. S., Mukhopadhyay, S., Mantovani, A., & Gordon, S. (2007). Macrophage receptors implicated in the “adaptive” form of innate immunity. Microbes and Infection. 9(14-15), 1680-1687. https://doi.org/10.1016/j.micinf.2007.09.002
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. In LWT - Food Science and Technology. 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Bucić-Kojić, A., Planinić, M., Tomas, S., Bilić, M., & Velić, D. (2007). Study of solid-liquid extraction kinetics of total polyphenols from grape seeds. Journal of Food Engineering. 81(1), 236-242. https://doi.org/10.1016/j.jfoodeng.2006.10.027
Campos, J. F., de Toledo Espindola, P. P., Torquato, H. F. V., Vital, W. D., Justo, G. Z., Silva, D. B., Carollo, C. A., Souza, K. de P., Paredes-Gamero, E. J., & dos Santos, E. L. (2017). Leaf and root extracts from Campomanesia adamantium (Myrtaceae) promote apoptotic death of leukemic cells via activation of intracellular calcium and caspase-3. Frontiers in Pharmacology. 8, 466. https://doi.org/10.3389/fphar.2017.00466
Carneiro, R. G. S., Castro, A. C., & Isaias, R. M. S. (2014). Unique histochemical gradients in a photosynthesis-deficient plant gall. South African Journal of Botany. 92, 97-104. https://doi.org/10.1016/j.sajb.2014.02.011
Chai, J., Jiang, P., Wang, P., Jiang, Y., Li, D., Bao, W., Liu, B., Liu, B., Zhao, L., Norde, W., Yuan, Q., Ren, F., & Li, Y. (2018). The intelligent delivery systems for bioactive compounds in foods: Physicochemical and physiological conditions, absorption mechanisms, obstacles and responsive strategies. Trends in Food Science and Technology, 78(June), 144–154. https://doi.org/10.1016/j.tifs.2018.06.003
Coussens, L. M., & Werb, Z. (2002). Inflammation and cancer. In Nature. 420(6917), 860-867. https://doi.org/10.1038/nature01322
Coutinho, I. D., Coelho, R. G., Kataoka, V. M. F., Honda, N. K., Silva, J. R. M., Vilegas, W., & Cardoso, C. A. L. (2008). Determination of phenolic compounds and evaluation of antioxidant capacity of Campomanesia adamantium leaves. Ecletica Quimica. 33, 53-60. https://doi.org/10.1590/S0100-46702008000400007
Cunha, E. B. B., DA SILVA, N. F., de Lima, J., Serrato, J. A., Aita, C. A. M., & Herai, R. H. (2020). Leaf extracts of campomanesia xanthocarpa positively regulates atherosclerotic-related protein expression. Anais Da Academia Brasileira de Ciencias, 92(4), 1–13. https://doi.org/10.1590/0001-3765202020191486
de Melo, L. V., & Sawaya, A. C. H. F. (2015). UHPLC–MS quantification of coumarin and chlorogenic acid in extracts of the medicinal plants known as guaco (Mikania glomerata and mikania laevigata). Brazilian Journal of Pharmacognosy. 25, 105-110. https://doi.org/10.1016/j.bjp.2015.02.005
de Oliveira Fernandes, T., de Ávila, R. I., de Moura, S. S., de Almeida Ribeiro, G., Naves, M. M. V., & Valadares, M. C. (2015). Campomanesia adamantium (Myrtaceae) fruits protect HEPG2 cells against carbon tetrachloride-induced toxicity. Toxicology Reports. 2, 184-193. https://doi.org/10.1016/j.toxrep.2014.11.018
de Oliveira Raphaelli, C., Pereira, E. dos S., Camargo, T. M., Ribeiro, J. A., Pereira, M. C., Vinholes, J., Dalmazo, G. O., Vizzotto, M., & Nora, L. (2021). Biological activity and chemical composition of fruits, seeds and leaves of guabirobeira (Campomanesia xanthocarpa O. Berg – Myrtaceae): A review. Food Bioscience, 40(July 2020). https://doi.org/10.1016/j.fbio.2021.100899
Do Amaral, M. D. P. H., Piresvieira, F., Leite, M. N., Do Amaral, L. H., Pinheiro, L. C., Fonseca, B. G., Pereira, M. C. S., & Varejão, E. V. (2009). Coumarin content of guaco syrup stored at different temperatures [Determinação do teor de cumarina no xarope de guaco armazenado em diferentes temperaturas]. Brazilian Journal of Pharmacognosy.19(2B), 607-611.
do Amarante, C. V. T., de Souza, A. G., Benincá, T. D. T., & Steffens, C. A. (2017). Phenolic content and antioxidant activity of fruit of Brazilian genotypes of feijoa. Pesquisa Agropecuaria Brasileira. 52, 1223-1230. https://doi.org/10.1590/S0100-204X2017001200011
Donado-Pestana, C. M., Belchior, T., Festuccia, W. T., & Genovese, M. I. (2015). Phenolic compounds from cambuci (Campomanesia phaea O. Berg) fruit attenuate glucose intolerance and adipose tissue inflammation induced by a high-fat, high-sucrose diet. Food Research International. 69, 170-178. https://doi.org/10.1016/j.foodres.2014.12.032
Ferreira, L. C., Grabe-Guimarães, A., De Paula, C. A., Michel, M. C. P., Guimarães, R. G., Rezende, S. A., De Souza Filho, J. D., & Saúde-Guimarães, D. A. (2013). Anti-inflammatory and antinociceptive activities of Campomanesia adamantium. Journal of Ethnopharmacology. 14 (1), 100-108. https://doi.org/10.1016/j.jep.2012.10.037
Figueirôa, E. D. O., Nascimento Da Silva, L. C., De Melo, C. M. L., Neves, J. K. D. A. L., Da Silva, N. H., Pereira, V. R. A., & Correia, M. T. D. S. (2013). Evaluation of antioxidant, immunomodulatory, and cytotoxic action of fractions from eugenia uniflora L. and eugenia malaccensis L.: Correlation with polyphenol and flavanoid content. The Scientific World Journal. 2013 (2013), 125027. https://doi.org/10.1155/2013/125027
Gobbo-Neto, L., & Lopes, N. P. (2007). Medicinal plants: Factors of influence on the content of secondary metabolites. Quimica Nova. 30, 374-381. https://doi.org/10.1590/S0100-40422007000200026
Hmoteh, J., Musthafa, K. S., & Voravuthikunchai, S. P. (2018). Effects of Rhodomyrtus tomentosa extract on virulence factors of Candida albicans and human neutrophil function. Archives of Oral Biology. 87, 35-42. https://doi.org/10.1016/j.archoralbio.2017.11.007
Klafke, J. Z., da Silva, M. A., Panigas, T. F., Belli, K. C., de Oliveira, M. F., Barichello, M. M., Rigo, F. K., Rossato, M. F., Soares dos Santos, A. R., Pizzolatti, M. G., Ferreira, J., & Viecili, P. R. N. (2010). Effects of Campomanesia xanthocarpa on biochemical, hematological and oxidative stress parameters in hypercholesterolemic patients. Journal of Ethnopharmacology, 12 (2), 299–305. https://doi.org/10.1016/j.jep.2009.11.004
Levy, A. S., & Carley, S. K. (2012). Cytotoxic activity of hexane extracts of psidium guajava L (Myrtaceae) and cassia alata L (Caesalpineaceae) in kasumi-1 and OV2008 cancer cell lines. Tropical Journal of Pharmaceutical Research. 11(2), 201-207. https://doi.org/10.4314/tjpr.v11i2.5
Lichtenthaler, H. K. (1987). Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology. 148, 350-382. https://doi.org/10.1016/0076-6879(87)48036-1
Malta, L. G., Tessaro, E. P., Eberlin, M., Pastore, G. M., & Liu, R. H. (2013). Assessment of antioxidant and antiproliferative activities and the identification of phenolic compounds of exotic Brazilian fruits. Food Research International. 53(1), 417-425. https://doi.org/10.1016/j.foodres.2013.04.024
Markman, B. E. O., Bacchi, E. M., & Kato, E. T. M. (2004). Antiulcerogenic effects of Campomanesia xanthocarpa. Journal of Ethnopharmacology, 94(1), 55–57. https://doi.org/10.1016/j.jep.2004.04.025
Michel, M. C. P., Guimarães, A. G., Paula, C. A., Rezende, S. A., Sobral, M. E. G., & Guimarães, D. A. S. (2013). Extracts from the leaves of Campomanesia velutina inhibits production of LPS/INF-γ induced inflammatory mediators in J774A.1 cells and exerts anti-inflammatory and antinociceptive effects in vivo. Brazilian Journal of Pharmacognosy, 23(6), 927–936. https://doi.org/10.1590/S0102-695X2013000600010
Müller, L., Fröhlich, K., & Böhm, V. (2011). Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay. Food Chemistry. 129(1), 139-148. https://doi.org/10.1016/j.foodchem.2011.04.045
Nanditha, B., & Prabhasankar, P. (2009). Antioxidants in bakery products: A review. 49(1), 1-27. Critical Reviews in Food Science and Nutrition. https://doi.org/10.1080/10408390701764104
Neha, K., Haider, M. R., Pathak, A., & Yar, M. S. (2019). Medicinal prospects of antioxidants: A review. 178, 687-704. European Journal of Medicinal Chemistry, 178, 687–704. https://doi.org/10.1016/j.ejmech.2019.06.010
Neri-Numa, I. A., Carvalho-Silva, L. B., Morales, J. P., Malta, L. G., Muramoto, M. T., Ferreira, J. E. M., de Carvalho, J. E., Ruiz, A. L. T. G., Maróstica Junior, M. R., & Pastore, G. M. (2013). Evaluation of the antioxidant, antiproliferative and antimutagenic potential of araçá-boi fruit (Eugenia stipitata Mc Vaugh - Myrtaceae) of the Brazilian Amazon Forest. Food Research International. 50(1), 70-76https://doi.org/10.1016/j.foodres.2012.09.032
Neri-Numa, I. A., Soriano Sancho, R. A., Pereira, A. P. A., & Pastore, G. M. (2018). Small Brazilian wild fruits: Nutrients, bioactive compounds, health-promotion properties and commercial interest. Food Research International, 103(May 2017), 345–360. https://doi.org/10.1016/j.foodres.2017.10.053
Park, H. J., Yang, H. J., Kim, K. H., & Kim, S. H. (2015). Aqueous extract of Orostachys japonicus A. Berger exerts immunostimulatory activity in RAW 264.7 macrophages. Journal of Ethnopharmacology. 170,210-217. https://doi.org/10.1016/j.jep.2015.04.012
Pipe, R. K., Farley, S. R., & Coles, J. A. (1997). The separation and characterisation of haemocytes from the mussel Mytilus edulis. Cell and Tissue Research. 289(3), 537-545. https://doi.org/10.1007/s004410050899
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26(9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Rein, M. J., Renouf, M., Cruz-Hernandez, C., Actis-Goretta, L., Thakkar, S. K., & da Silva Pinto, M. (2013). Bioavailability of bioactive food compounds: A challenging journey to bioefficacy. British Journal of Clinical Pharmacology, 75(3), 588–602. https://doi.org/10.1111/j.1365-2125.2012.04425.x
Rocha, W. S., Lopes, R. M., da Silva, D. B., Vieira, R. F., da Silva, J. P., & Agostini-Costa, T. da S. (2011). Total phenolics and condensed tannins in native fruits from Brazilian savanna. Revista Brasileira de Fruticultura. 33, 1215-1221. https://doi.org/10.1590/S0100-29452011000400021
Rosen, H., & Gordon, S. (1987). Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. Journal of Experimental Medicine. 166(6), 1685-1701. https://doi.org/10.1084/jem.166.6.1685
Salmazzo, G. R., Verdan, M. H., Silva, F., Cicarelli, R. M., Mota, J. da S., Salvador, M. J., de Carvalho, J. E., & Cardoso, C. A. L. (2019). Chemical composition and antiproliferative, antioxidant and trypanocidal activities of the fruits from Campomanesia xanthocarpa (Mart.) O. Berg (Myrtaceae). Natural Product Research, 0(0), 1–5. https://doi.org/10.1080/14786419.2019.1607333
Santos, M. D. S., Lima, J. J. de, Petkowicz, C. L. D. O., & Cândido, L. M. B. (2013). Chemical characterization and evaluation of the antioxidant potential of gabiroba jam (Campomanesia xanthocarpa Berg). Acta Scientiarum. Agronomy. 35, 73-82. https://doi.org/10.4025/actasciagron.v35i1.14389
Santos, M. da S., Miguel, O. G., Petkowicz, C. L. O., & Cândido, L. M. B. (2012). Antioxidant and fatty acid profile of gabiroba seed (Campomanesisa Xanthocarpa Berg). Food Science and Technology. 32, 234-238. https://doi.org/10.1590/s0101-20612012005000045
Seigler, D. S., Seilheimer, S., Keesy, J., & Huang, H. F. (1986). Tannins from four commonAcacia species of Texas and Northeastern Mexico. Economic Botany. 40, 220-232. https://doi.org/10.1007/bf02859146
Skalicka-Woźniak, K., & Głowniak, K. (2012). Pressurized liquid extraction of coumarins from fruits of Heracleum leskowii with application of solvents with different polarity under increasing temperature. Molecules. 17 (4), 4133-41. https://doi.org/10.3390/molecules17044133
Souza, A., Fassina, A. C., & Saraiva, F. (2018). Compostos bioativos e atividade antioxidante em frutas nativas do Brasil. Agrotrópica (Itabuna). 30 (1), 73-78. https://doi.org/10.21757/0103-3816.2018v30n1p73-78.pdf
Stewart, P., Boonsiri, P., Puthong, S., & Rojpibulstit, P. (2013). Antioxidant activity and ultrastructural changes in gastric cancer cell lines induced by Northeastern Thai edible folk plant extracts. BMC Complementary and Alternative Medicine. 13(1), 1-11. https://doi.org/10.1186/1472-6882-13-60
Stockert, J. C., Horobin, R. W., Colombo, L. L., & Blázquez-Castro, A. (2018). Tetrazolium salts and formazan products in Cell Biology: Viability assessment, fluorescence imaging, and labeling perspectives. Acta Histochemica, 120(3), 159–167. https://doi.org/10.1016/j.acthis.2018.02.005
Teixeira, N., Melo, J. C. S., Batista, L. F., Paula-Souza, J., Fronza, P., & Brandão, M. G. L. (2019). Edible fruits from Brazilian biodiversity: A review on their sensorial characteristics versus bioactivity as tool to select research. Food Research International, 119(October 2018), 325–348. https://doi.org/10.1016/j.foodres.2019.01.058
Viecili, P. R. N., Borges, D. O., Kirsten, K., Malheiros, J., Viecili, E., Melo, R. D., Trevisan, G., da Silva, M. A., Bochi, G. V., Moresco, R. N., & Klafke, J. Z. (2014). Effects of Campomanesia xanthocarpa on inflammatory processes, oxidative stress, endothelial dysfunction and lipid biomarkers in hypercholesterolemic individuals. Atherosclerosis, 234(1), 85–92. https://doi.org/10.1016/j.atherosclerosis.2014.02.010
Vinagre, A. S., Rönnau, Â. D. S. R. O., Pereira, S. F., Da Silveira, L. U., Wiilland, E. D. F., & Suyenaga, E. S. (2010). Anti-diabetic effects of Campomanesia xanthocarpa (Berg) leaf decoction. Brazilian Journal of Pharmaceutical Sciences. 46, 169-177. https://doi.org/10.1590/S1984-82502010000200002
Wang, W. H., Tyan, Y. C., Chen, Z. S., Lin, C. G., Yang, M. H., Yuan, S. S., & Tsai, W. C. (2014). Evaluation of the antioxidant activity and antiproliferative effect of the jaboticaba (Myrciaria cauliflora) seed extracts in oral carcinoma cells. BioMed Research International. 2014 (2014), 185946. https://doi.org/10.1155/2014/185946
Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 64(4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Viviane Dalastra; Caroline Kael; Sandro José Ribeiro Bonatto; Ricardo Key Yamazaki
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
