Evaluation of the antibacterial activity of a dentin biomodifier based on Copaifera multijulga Hayne oil
Keywords:Copaifera; Microbiology; Phytotherapy; Dental materials.
Objectives: To evaluate in vitro the antibacterial activity of a dentin biomodifier based on copaiba oil. Methodology: The antibacterial activity test was carried out over a period of 12 months, where the emulsion was stored in different environments and analysed at 0, 3 and 12 months. For that, the microorganisms Streptococcus mutans and Lactobacillus paracasei were used. The positive control was 2% chlorhexidine digluconate and the negative control was the emulsion vehicles: distilled water, 0.5% diazolidinyl urea and 1% polysorbate 80. 96-well microplates were used, so that each well had a final volume of 100 µL. On the microplate, the columns were distributed in the numbers from 1 to 12 and the lines in the letters “A” to “G”. In the wells, the emulsion was distributed at different concentrations, in addition to the negative control and positive control. Results: The emulsion showed bactericidal or bacteriostatic activity against all microorganisms tested at all concentrations for the microorganisms S. mutans and L. paracasei at time zero. After 12 months, the emulsion formulation based on copaiba oil continued to exert bactericidal and bacteriostatic activity at concentrations of 26.66 μL/mL and 15 μL/mL, respectively, against the microorganism S. mutans. The refrigerator environment showed bacteriostatic activity, while the air conditioning environment showed bactericidal activity against S. mutans in the period of 12 months. Conclusion: The biomodifier showed bactericidal and/or bacteriostatic activity in different environments for 12 months, and can be stored in a refrigerator.
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Copyright (c) 2022 Geisy Rebouças Lima Brasil ; Laryssa da Silva Cavalcante ; Cristiane Nagai de Bari; Marne Carvalho de Vasconcellos ; Nikeila Chacon de Oliveira Conde; Carina Toda; Simone Assayag Hanan; Maria Fulgência Costa Lima Bandeira
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