Bioactives Derived from Endophytic Fungi Isolated from Azadirachta Indica (Neem) and their Applications

Authors

DOI:

https://doi.org/10.33448/rsd-v11i9.32454

Keywords:

Bioprospecting; Secondary metabolites; Micro-organisms; Medicinal plants.

Abstract

Azadirachta indica A. Juss (Neen) has great ethnomedicinal importance, being known for its insecticidal power and for various therapeutic purposes as a folk medicine. Studies show that endophytes isolated from medicinal plants have potential as an alternative source of secondary metabolites. More than 400 compounds have been isolated from different parts of Neen, including important bioactive secondary metabolites such as azadirachtin, nimbidin, nimbin, nimbolide, gedunin, however, the metabolites of fungi already isolated and characterized showed that more than half of the molecules have antibacterial activity, antifungal and antitumor. Penicillin, cephalosporin, ergotrate and statins are fungal secondary metabolites. The present work aimed to carry out a review of the literature on bioactive products from endophytic fungi of Azadirachta indica and their applications. Activities from extracts of plant parts (leaves, stems, roots) or from cultivation and selection of endophytes other than the kingdom Fungi were excluded. 11 articles were found in the databases that present 12 different endophytic fungi, isolated from Azadirachta indica, with the production of different secondary metabolites and varied applications. Antimicrobial, antifungal, insecticidal and nematicidal activities and improvement in plant adaptation mechanisms. Thirty-four metabolites were identified and tested in different techniques and applications.

Author Biographies

José Celso Rocha Martins Júnior, Universidade Católica Dom Bosco

Postgraduate Program in Environmental Sciences and Agricultural Sustainability

Vanessa Pinto Oleques Pradebon, Universidade Católica Dom Bosco

Postgraduate Program in Environmental Sciences and Agricultural Sustainability

Laís Rezende Maia, Universidade Católica Dom Bosco

Postgraduate Program in Environmental Sciences and Agricultural Sustainability

Antonia Railda Roel, Universidade Católica Dom Bosco

Programa de Pós Graduação em Ciencias Ambientais e Sustentabilidade Agropecuária

Tiago Tognolli de Almeida, Universidade Católica Dom Bosco

Postgraduate Program in Environmental Sciences and Agricultural Sustainability

References

Almeida, T. T.,Ribeiro, M. A. dos S., Polonio, J. C. Garcia, F. PNakamura, C. V. Meurer, E. C., Sarragiotto, M. H., Baldoqui, D. C., Azevedo, J. L., Pamphile, J. A. (2017). Curvulinandspirostaphylotrichins R and U from extracts produced sp. associatedtoaquaticmacrophyteswithantileishmanialactivity. Natural Product Research, 32(23), 2783–2790. https://doi.org/10.1080/14786419.2017.1380011

Alves, D. R. Silva, W. M. B. da, Santos, D. L. dos, Freire, F. das C. de O., Vasconcelos, F. R., & Morais, S. M. de. (2020). Atividades Antioxidante, Anticolinesterasica e Citotóxica de Metabolitos de Fungos Endofiticos / Antioxidant, AnticolinesterasicAndCytoxicActivitiesofEndophyticFungusMetabolites. Brazilian Journal Development, 6(9), 73684–73691. https://doi.org/10.34117/bjdv6n9-721

Benelli, G., Canale, A., Toniolo, C., Higuchi, A., Murugan, K Pavela, R., Nicoletti, M. (2016). Neem (Azadirachta indica): towards the ideal insecticide? Natural Product Research, 31(4), 369–386. https://doi.org/10.1080/14786419.2016.1214834

Berendsen, RL, Pieterse, CMJ, & Bakker, PAHM (2012). O microbioma da rizosfera e a saúde das plantas. Trends in Plant Science , 17 (8), 478–486. https://doi.org/10.1016/j.tplants.2012.04.001

Bertalan, M., Albano, R., de Pádua, V., Rouws, L., Rojas, C., Hemerly, A., Teixeira, K., Schwab, S., Araujo, J., Oliveira, A., França, L., Magalhães, V., Alquéres, S., Cardoso, A., Almeida, W., Loureiro, M., Nogueira, E., Cidade, D., Oliveira, D., & Simão, T. (2009). Complete genome sequence of the sugar cane nitrogen-fixing endophytic Gluconacetobacter diazotrophicus Pal5. BMC Genomics, 10(1), 450. https://doi.org/10.1186/1471-2164-10-450

Bibi, S. N., Gokhan, Z., Rajesh, J., & Mahomoodally, M. F. (2020). Fungal Endophytes Associated With mangroves – Chemistry And Biopharmaceutical Potential. South African Journal of Botany. https://doi.org/10.1016/j.sajb.2019.12.016

Bungihan, ME, Tan, MA, Kitajima, M., Kogure, N., Franzblau, SG, Dela Cruz, TEE, Takayama, H., & Nonato, MG (2011). Metabólitos bioativos de Diaporthe sp. P133, um fungo endofítico isolado de Pandanusamaryllifolius. Journal Of Natural Medicines , 65 (3-4), 606-609. https://doi.org/10.1007/s11418-011-0518-x

Calderani, F. A. Orlandelli, R. C., Pamphile, J. A. (2016). Compostos Bioativos com Propriedades Antitumorais Produzidos por Fungos Endofíticos. Uningá Review, 25(2). https://revista.uninga.br/uningareviews/article/view/1756

Chatterjee, A., & Pakrashi, S. C. (1991). TreatiseonIndian medicinal plants. Publications&InformationDirectorate.

Chutulo, E. C., & Chalannavar, R. K. (2018). EndophyticMycofloraandTheirBioActiveCompoundsfromAzadirachta Indica: A Comprehensive Review. Journal of Fungi, 4(2), 42. https://doi.org/10.3390/jof4020042

Dettrakul, S., Kittakoop, P., Isaka, M., Nopichai, S., Suyarnsestakorn, C., Tanticharoen, M., & Thebtaranonth, Y. (2003). AntimycobacterialpimaranediterpenesfromtheFungusDiaporthe sp. Bioorganic & Medicinal Chemistry Letters, 13(7), 1253–1255. https://doi.org/10.1016/s0960-894x(03)00111-2

Gupta, S.C., Prasad, S., Tyagi, A.K., Kunnumakkara, A.B., & Aggarwal, B.B. (2017). Neem (Azadirachta indica): Uma panaceia tradicional indiana com base molecular moderna. Fitomedicina , 34 , 14-20.

Hardoim, P. R., Van Overbeek, L. S., Berg, G., Pirttilä, A. M., Compant, S., Campisano, A., ... &Sessitsch, A. (2015). The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology And molecular biology reviews, 79(3), 293-320.

Jam Ashkenazi, S., & Fotouhi Far, K.-B. (2017). Diversity Of Endophytic Fungi Of common yew (Taxus Baccata L.) in Iran. Mycological Progress, 16(3), 247–256. https://doi.org/10.1007/s11557-017-1274-4

Keller, N. P., Turner, G., & Bennett, J. W. (2005). Fungal Secondary Metabolism — from biochemistry to genomics. Nature Reviews Microbiology, 3(12), 937–947. https://doi.org/10.1038/nrmicro1286

Kharwar, R. N., Verma, V. C., Kumar, A., Gond, S. K., Harper, J. K., Hess, W. M., ... & Strobel, G. A. (2009). Javanicin, anantibacterialnaphthaquinonefromanendophyticfungusofneem, Chloridium sp. Current Microbiology, 58(3), 233-238.

Kharwar, R. N., Sharma, V. K., Mishra, A., Kumar, J., Singh, D. K., Verma, S. K., Gond, S. K., Kumar, A., Kaushik, N., Revuru, B., &Kusari, S. (2020). HarnessingthePhytotherapeuticTreasure Troves of the Ancient Medicinal PlantAzadirachta indica (Neem)and Associated EndophyticMicroorganisms. Planta Medica, 86(13/14), 906–940. https://doi.org/10.1055/a-1107-9370

Kumari, P., Singh, A., Singh, D. K., Sharma, V. K., Kumar, J., Gupta, V. K., Bhattacharya, S., &Kharwar, R. N. (2021). IsolationandpurificationofbioactivemetabolitesfromanendophyticfungusPenicilliumcitrinumofAzadirachta indica. South AfricanJournalofBotany, 139, 449–457. https://doi.org/10.1016/j.sajb.2021.02.020

Kusari, S., Verma, V. C., Lamshoeft, M., & Spiteller, M. (2011). An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin. World Journal of Microbiology and Biotechnology, 28(3), 1287–1294. https://doi.org/10.1007/s11274-011-0876-2

Li, G. H., Yu, Z. F., Li, X., Wang, X. B., Zheng, L. J., & Zhang, K. Q. (2007). NematicidalmetabolitesproducedbytheendophyticfungusGeotrichum sp. AL4. Chemistry & Biodiversity, 4(7), 1520–1524. https://doi.org/10.1002/cbdv.200790131

Ludwig-Müller, J. (2015). Plants And Endophytes: equal partners in secondary metabolite production? BiotechnologyLetters, 37(7), 1325–1334. https://doi.org/10.1007/s10529-015-1814-4

Montel, J. V. F., De Moraes, G. K. A., Dantas, S. B. de S., &Chapla, V. M. (2021). Caracterização química de extratos brutos obtidos de dois fungos endofíticos isolados de Cochlospermum regium / Chemical characterization of crude extracts obtained from two endophytic fungi isolated from Cochlospermum regium. Brazilian Journal of Development, 7(4), 42728–42737. https://doi.org/10.34117/bjdv7n4-639

Nisa, H., Kamili, A. N., Nawchoo, I. A., Shafi, S., Shameem, N., & Bandh, S. A. (2015). Fungal Endophytes as prolific source phytochemicals and other bioactive natural products: A review. Microbial Pathogenesis, 82, 50–59. https://doi.org/10.1016/j.micpath.2015.04.001

Oliveira, J. A. S., & Gonçalves Nogueira, D. (2022). FUNGOS ENDOFÍTICOS DIAPORTHE: UMA FONTE PROMISSORA DE METABÓLITOS PARA A ÁREA DA SAÚDE. Arquivos Do Mudi, 26(1), 39–50. https://doi.org/10.4025/arqmudi.v26i1.61089

Pasrija, P., Girdhar, M., Kumar, M., Arora, S., & Katyal, A. (2022). “Endophytes: and unexplored treasure to combat Multidrug resistance." Phytomedicine Plus, 2(2), 100249. https://doi.org/10.1016/j.phyplu.2022.100249

Pelaéz, F. (2005) Biologicalactivitiesoffungalmetabolites. In: An Z (ed), Handbook of Industrial Mycology. Marcel Dekker, New York, p. 49-92.

Pinheiro, J. B. Polonio, J. C. Orlandelli, R. C., Pamphile, J. A., & Golias, H. C. (2020). Atividade larvicida de fungos endofíticos: uma revisão. Brazilian Journal of Development, 6(6), 35761–35774. https://doi.org/10.34117/bjdv6n6-205

Rajagopal, K., &Rajagopal, K. (2011). ExtractionandcharacterizationofmelaninfromPhomopsis: A phellophyticfung Isolated from Azadirachta indica A. Juss. AfricanJournalofMicrobiologyResearch, 5(7), 762–766. https://doi.org/10.5897/ajmr10.514

Rashid, S., Charles, TC, & Glick, BR (2012). Isolamento e caracterização de novos endófitos bacterianos promotores de crescimento de plantas. Ecologia Aplicada do Solo , 61 , 217–224. https://doi.org/10.1016/j.apsoil.2011.09.011

Reddy, I. V. S., & Neelima, P. (2022). Neem (Azadirachta indica): A Review on Medicinal Kalpavriksha. International Journal of Economic Plants, 9(1), 059–063. https://doi.org/10.23910/2/2021.0437d

Ruma, K., Shailasree, S., Sampath, K. K., Niranjana, S. R., & Prakash, H. S. (2011). Diversionoffungalendophytesfromtwoendemictreespeciesartocarpushirsutuslam. and bateria indica linn. of Western Ghats, India. World Journal of Agricultural Sciences, 7(5), 577-582.

Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, Ma., & Glick, B. R. (2016). Plant Growth-promoting bacterial endophytes. Microbiological Research, 183, 92–99. https://doi.org/10.1016/j.micres.2015.11.008

Shao-hua, W. U., You-wei, C. H. E. N., Shi-cheng, S. H. A. O., Zhi-ying, L. I., Li-yuan, Y. A. N. G., & Shao-lan, L. I. (2009). Studiesonthe Metabolites of Aspergillus aculeatus YM 311498, an Endophytic Fungus from Azadirachta indica A. Juss. Natural Product Research Development, 21(5).

Strobel, G., & Daisy, B. (2003). Bioprospecting for Microbial EndophytesandTheir Natural Products. Microbiologyand Molecular Biology Reviews, 67(4), 491–502. https://doi.org/10.1128/mmbr.67.4.491-502.2003

Tejesvi, M. V., Mahesh, B., Nalini, M. S., Prakash, H. S., Kini, K. R., Subbiah, V., & Shetty, H. S. (2006). Fungalendophyteassemblagesfromethnopharmaceuticallyimportant medicinal trees. CanadianJournalofMicrobiology, 52(5), 427–435. https://doi.org/10.1139/w05-143

Trivedi, P., Leach, J. E., Tringe, S. G., Sa, T., & Singh, B. K. (2020). Plant-microbiome interactions: fromcommunityassemblytoplanthealth. Nature Reviews. Microbiology, 18(11), 607–621. https://doi.org/10.1038/s41579-020-0412-1

Verma, V. C., Gond, S. K., Kumar, A., Kharwar, R. N., & Strobel, G. (2007). The Endophytic Mycoflora Bark, Leaf, and Stem Tissues of Azadirachta indica A. Juss (Neem) from Varanasi (India). Microbial Ecology, 54(1), 119–125. https://doi.org/10.1007/s00248-006-9179-9

Verma, V. C., Singh, S. K., &Kharwar, R. N. (2012). Histological Investigation FungalEndophytes in HealthyTissuesofAzadirachta indica A. Juss. Agriculture And Natural Resources, 46(2), 229-237.

Wani, Z. A., Ashraf, N., Mohiuddin, T., & Riyaz-Ul-Hassan, S. (2015). Plant-endophyte symbiosis, an ecological perspective. Applied Microbiology Biotechnology, 99(7), 2955–2965. https://doi.org/10.1007/s00253-015-6487-3

Wu, S.-H., Chen, Y.-W., & Miao, C.-P. (2011). Secondary metabolites of endophytic fungus Xylaria sp. YC-10 of Azadirachta indica. Chemistry Of Natural Compounds, 47(5), 858–861. https://doi.org/10.1007/s10600-011-0086-z

Xuan, Q.-C., Huang, R., Miao, C.-P., Chen, Y.-W., Zhai, Y.-Z., Song, F., Wang, T., & Wu, S.-H. (2014). Secondary Metabolites of Endophytic Fungus Trichoderma sp. YM 311505 of Azadirachta indica. Chemistry Of Natural Compounds, 50(1), 139–141. https://doi.org/10.1007/s10600-014-0891-2

Yan, X., Sikora, RA, & Zheng, J. (2011). Potencial uso de fungos endofíticos de pepino (Cucumis Sativus L.) como agentes de tratamento de sementes contra o nematoide das galhas Meloidogyne Incognita. Jornal da Universidade de Zhejiang CIÊNCIA B , 12 (3), 219–225. https://doi.org/10.1631/jzus.b1000165

Published

20/07/2022

How to Cite

RICCI, A. P.; MARTINS JÚNIOR, J. C. R.; PRADEBON, V. P. O.; MAIA, L. R.; LOOSLI , A. W. M. .; ROEL, A. R.; ALMEIDA, T. T. de. Bioactives Derived from Endophytic Fungi Isolated from Azadirachta Indica (Neem) and their Applications. Research, Society and Development, [S. l.], v. 11, n. 9, p. e13911932454, 2022. DOI: 10.33448/rsd-v11i9.32454. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/32454. Acesso em: 26 nov. 2024.

Issue

Section

Health Sciences